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Sequence Analysis of Cochlodinium polykrikoides Isolated from Korean Coastal Waters Using Sequences of Internal Transcribed Spacers and 5.8S rDNA

  • Kim, Hak-Gyoon;Cho, Yong-Chul;Cho, Eun-Seob
    • Journal of the korean society of oceanography
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    • v.35 no.3
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    • pp.158-160
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    • 2000
  • The relativity of four isolates of C. polykrikoides was determined by comparative sequence analysis based on direct sequencing of PCR amplified ribosomal DNA (the internal transcribed spacer region and the 5.8S rDNA). Sequence comparisons indicated that four isolates had the same nucleotide sites in the ITS regions, as well as a total of 585 nucleotide length and 100% homology. The molecular data revealed that C. polykrikoides in Korean coastal waters show no genetical difference.

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Isolation of the Inositol Phosphoceramide Synthase Gene (AUR1) from Stress-Tolerant Yeast Pichia kudriavzevii

  • Yoo, Boung-Hyuk;Kim, Myoung-Dong
    • Journal of Microbiology and Biotechnology
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    • v.25 no.11
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    • pp.1902-1907
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    • 2015
  • This study is the first report of the entire nucleotide sequence of an inositol phosphoceramide synthase gene from the stress-tolerant yeast Pichia kudriavzevii (PkAUR1). Sequence analysis revealed an open reading frame that spans 1,443 bp and encodes a 480-amino-acid-residue protein with the highest sequence similarity (41.7%) to Aur1 from Spathaspora passalidarum. A phenotypic assay with transformed S. cerevisiae and P. kudriavzevii indicated that two amino acid residues, Phe166 and Gly249, play crucial roles in the resistance to aureobasidin A, which is consistent with previous reports for other fungal Aur1s. The GenBank Accession No. for PkAUR1 is KP729614.

New Constructions of Binary LCZ Sequence Sets With Flexible LCZ and Set Size (유연한 LCZ와 집합 크기를 갖는 새로운 이진 LCZ 수열 집합의 생성)

  • Kim, Young-Sik;Jang, Ji-Woong;No, Jong-Seon;Chung, Ha-Bong
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.32 no.3C
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    • pp.226-233
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    • 2007
  • In this paper, we construct new LCZ sequence sets with parameters $(2^{n+1}-2,M,L,2)$. In this scheme, we can relatively freely choose the LCZ length L and the resulting LCZ sequence set has the size in which is nearly optimal with respect to Tang, Fan, and Matsufuji bound.

OPTINAL SCHEDULING OF IDEALIZED MULTI-PRODUCT BATCH OPERATION

  • Lee, In-Beum;Chang, Kun-Soo
    • 제어로봇시스템학회:학술대회논문집
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    • 1989.10a
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    • pp.825-827
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    • 1989
  • A heuristic model which determines the scheduling of serial flowshops with minimization of the makespan is proposed for an idealized batch chemical plant. It generates an initial sequence by heuristic reasoning and improves it recursively until no improvement is possible. The heuristic reasoning is based on Johnson's Rule which gives the sequence with the minimum makespan for a two-unit flowshop. The evolutionary step searches the neighborhood of the current sequence for sequences with lower makespan. The robustness of this model is also examined by comparing the minimum makespan of literature examples with the theoretical one.

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Kinematic Sequence Patterns according to Movement Time of Choku-tsuki in Karate Kumite (카라테 구미테 정면 지르기의 동작수행시간에 따른 운동학적 시퀀스 패턴)

  • Kim, Tae-Whan;Kim, Mi-Sun;Kim, Joo-Nyeon
    • Korean Journal of Applied Biomechanics
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    • v.30 no.3
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    • pp.225-234
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    • 2020
  • Objective: The purpose of this study was to investigate the kinematic sequence patterns according to movement times during Karate choku-tsuki. Method: Ten Korea national Karate athletes participated in this study. Participants asked to perform jodan and chudan choku-tsuki. 30 infrared cameras were used to measure angular kinematic of elbow, shoulder, trunk, pelvis, hip, knee, ankle. Results: The two-way repeated measures ANOVA revealed significant effects for the joints (p<.05). But no significant effect for the movement time and interaction of joints x movement time existed for the kinematic sequence variables. Conclusion: For karate kumite players to reduce the movement time of punch, it is necessary to train kinematic sequences that allow each joint to rotate at a relatively similar timing.

Screening of Potential Stress-Responsive and Immune-Related Genes by Expressed Sequence Tags in Mud Loach (Misgurnus mizolepis)

  • Nam, Yoon-Kwon;Kim, Dong-Soo
    • Journal of fish pathology
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    • v.15 no.2
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    • pp.83-92
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    • 2002
  • EST analysis was performed to identify stress-responsive and immune-related genes from mud loach (Misgurnus mizolepis), cDNA libraries were constructed with liver, intestine and kidney tissues and randomly chosen clones (216 for liver, 198 for intestine and 224 for kidney) were subjected to automated sequence analysis. Of 638 clones sequenced in totlal, approximalely 25% of ESTs was novel sequences (no match to GenBank) or sequences with high homology to hypothrtical/unknown genes. Several potential stress-responsive biomarker and/or immure-related genes were identified in all the tissues examined. It included lectin, MHC class I/II proteins, proteinase inhibitors, superoxide dismulase, catalase, glutathionc-S. transferase, heat-shock protein, warm temperature acclimation protein, complements, methylrransferasc, zinc finger proteins, macrophage maturation associated protein, and others. This information will offer new possibilities as fundamental baseline data for the molecular genetics and breeding of this species with an emphasis on the development of stress. (and disease)-resistsnt fish.

Determination of Phylogenetic Relationships of Turkish Native Cattle Breeds with Other Cattle Breeds Using Mitochondrial DNA D-loop Sequence Polymorphism

  • Ozdemir, Memis;Dogru, Unsal
    • Asian-Australasian Journal of Animal Sciences
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    • v.22 no.7
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    • pp.955-961
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    • 2009
  • The aim of this study was to determine the specific polymorphic sites in cattle breeds and inter- and interbreed genetic variation among breeds and to develop a databank of Turkish native cattle mtDNA using sequence analysis. The entire D-loop region was analyzed based on DNA sequences in Turkish Grey, East Anatolian Red, South Anatolian Red, and Anatolian Black native breeds. In total, 68 nucleotide differences were observed at 26 different sites. The variable positions consisted of 22 transitions, two transversions, and two insertions, but no deletions. Haplotype number, haplotype diversity, nucleotide diversity, and mean number of pairwise difference values were found to be 17, 0.993, 0.00478, and 4.275, respectively. In addition, a phylogeny was developed by comparison among cattle populations for which the entire D-loop sequence was available. A high level of genetic variation was observed within and among the native cattle breeds.

Extracellular Production of Alpha-Interferon by Recombinant Escherichia coli: Part III. Gene Expression for Product Formation (유전자 재조합 대장균을 사용한 Alpha-Interferon의 생산과 분비: 제3부: Interferon생산을 위한 유전자의 발현)

  • 노갑수;최차용
    • KSBB Journal
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    • v.5 no.3
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    • pp.293-298
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    • 1990
  • Alpha-interferon was produced by using recombinant Escherichia coli strains, which carry cloned alpha-interferon gene in plasmid vectors, pIF-III-B and pIF-III-C. With the aid of signal sequence of E. coli lipoprotein, which is placed right in front of the upstream of the cloned alpha-interferon gene of the plasmids, about 50% of alpha-interferon produced was excreted or secreted. Meanwhile, there was no extracellular production of alpha-interferon from the recombinant strain carrying the plasmid Hif-2h that lacks the signal sequence of lipoprotein.

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An Improved Reinforcement Learning Technique for Mission Completion (임무수행을 위한 개선된 강화학습 방법)

  • 권우영;이상훈;서일홍
    • The Transactions of the Korean Institute of Electrical Engineers D
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    • v.52 no.9
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    • pp.533-539
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    • 2003
  • Reinforcement learning (RL) has been widely used as a learning mechanism of an artificial life system. However, RL usually suffers from slow convergence to the optimum state-action sequence or a sequence of stimulus-response (SR) behaviors, and may not correctly work in non-Markov processes. In this paper, first, to cope with slow-convergence problem, if some state-action pairs are considered as disturbance for optimum sequence, then they no to be eliminated in long-term memory (LTM), where such disturbances are found by a shortest path-finding algorithm. This process is shown to let the system get an enhanced learning speed. Second, to partly solve a non-Markov problem, if a stimulus is frequently met in a searching-process, then the stimulus will be classified as a sequential percept for a non-Markov hidden state. And thus, a correct behavior for a non-Markov hidden state can be learned as in a Markov environment. To show the validity of our proposed learning technologies, several simulation result j will be illustrated.

Modified Sturm Sequence Property for Damped Systems (감쇠시스템을 위한 개선된 Sturm 수열 성질)

  • Jo, Ji-Seong;Lee, Chong-Won;Lee, In-Won
    • Proceedings of the Computational Structural Engineering Institute Conference
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    • 2001.04a
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    • pp.34-41
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    • 2001
  • Most of the eigenvalue analysis methods for the undamped or proportionally damped systems use the well-known Sturm sequence property to check the missed eigenvalues when only a set of the lowest modes is to be used for large structures. However, in the case of the non-proportionally damped systems such as the soil-structure interaction system, the structural control system and the composite structures, no counterpart of the Sturm sequence property for undamped systems has been developed yet. Hence, when some important modes are missed for those systems, it may leads to poor results in dynamic analysis. In this paper, a technique for calculating the number of eigenvalues inside the open disk of arbitrary radius for the eigenproblem with the damping matrix is proposed by applying Chen's algorithm and Gleyse's theorem. To verify the applicability of the proposed method, two numerical examples are considered.

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