Sequence Analysis of Cochlodinium polykrikoides Isolated from Korean Coastal Waters Using Sequences of Internal Transcribed Spacers and 5.8S rDNA

  • Kim, Hak-Gyoon (Harmful Algal Research Division, National Fisheries Research and Development Institute) ;
  • Cho, Yong-Chul (Harmful Algal Research Division, National Fisheries Research and Development Institute) ;
  • Cho, Eun-Seob (Harmful Algal Research Division, National Fisheries Research and Development Institute)
  • Published : 2000.09.30

Abstract

The relativity of four isolates of C. polykrikoides was determined by comparative sequence analysis based on direct sequencing of PCR amplified ribosomal DNA (the internal transcribed spacer region and the 5.8S rDNA). Sequence comparisons indicated that four isolates had the same nucleotide sites in the ITS regions, as well as a total of 585 nucleotide length and 100% homology. The molecular data revealed that C. polykrikoides in Korean coastal waters show no genetical difference.

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