• Title/Summary/Keyword: Nitrogen-15

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A Study on Estimation of Minimum Protein Requirement (단백질(蛋白質) 최저요구량(最低要求量) 측정(測定)에 관한 연구(測定))

  • Wang, Soo-Kyung;Kim, Mi-Kyung
    • Journal of Nutrition and Health
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    • v.9 no.3
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    • pp.8-17
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    • 1976
  • This study was carried out to estimate the minimum protein requirement for the Korean college girls, and to see the effect of carbohydrate intake on the minimum protein requirement in the rat. In the first experiment, three students attending Ewha Womans University were given protein free diet for 7 days while caloric intake were adjusted to maintain constant body weight. Endogenous nitrogen excretion was measured to estimate their minimum protein requirement. Second experiment was carried out in 3 parts to find out the effect of carbohydrate intake on the minimum protein requirement using 15 female, 50 days old rats weighing 120g. Inexperiment 2-1, the rats were fed protein free diet for 9 days and endogenous nitrogen excretion was measured to determine the minimum protein requirement of the rats. In experiment 2-2, the minimum casein requirement of the rats needed to maintain nitrogen equilibrium was estimated by the least squares regression method feeding 3 different levels of casein determined from the result of experiment 2-1. Finally, in experiment 2-3, the rats were given the minimum casein requirement for 7 days with 3 different levels of carbohydrate to find out the effect of carbohydrate intake on the nitrogen balance. The results of this study were as follows. Experiment I. Daily endogenous nitrogen losses of 3 college girls were 1.89g in urine, 1.10g in feces and 0.0108g from skin since the total daily endogenous nitrogen loss was 3.0g and the total daily nitrogen intake was 0.52g, the minimum nitrogen requirement of these subjects was about 2.48g/day (15.5g protein/day). Experiment II. Experiment 2-1: daily endogenous nitrogen losses of the rats fed protein free diet were $22.63{\pm}0.48\;mg$ through urine and $13.12{\pm}0.47\;mg$ in feces. The total daily endogenous nitrogen ouput was $35.71{\pm}1.27\;mg$ and the minimum protein requirement of the rats was 225mg/day in this study. Experiment 2-2: the minimum casein requirement needed to maintain the rats in nitrogen equilibrium was determined to be 340mg/day. Experiment 2-3: the rats were given 350mg/day casein with 3 different levels of carbohydrate (12,26 or 46 Cal by sugar-cornstarch mixture). The nitrogen balances were measured to be +0.0021, +0.0073 and +0.0143 respectively and there were significant differences among 3 groups.

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Analytical studies on the Rice Yield Component and Yield in South Region of Korea. II. Variation in the rice yield component and yield under the different nitrogen fertilizing levels (남부지방에 있어서 수도수량구성요소 및 수량해석II. 질소시비량에 따른 주요형질 및 수량의 변이)

  • 김용재;김규진
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.29 no.3
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    • pp.218-226
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    • 1984
  • This study was conducted to establish fundamental of cultivation system in the southern warm region of Korea by investigation of variation of yield component and yield under the different nitrogen fertilizing levels. And that levels in this experiment were 10, 15, 20 and 25 Kg/10a. Optimum fertilizing level of nitrogen was 20 kg/10a in Seokwang variety and 25 kg/ 10a in Dongjin variety. On the optimum fertilizing level (Seokwang; 20 kg/10a, Dongjin; 15 kg/10a), the correlation coefficient between No. of panicles per unit area and the rate of ripened grains were showed negative correlation (Seokwang; r =-0.6023$\^$*/, Dongjin; r=-0.858l$\^$**/). In the primary, secondary branches and spikelets, the degeneration ratio was increased significantly under the level of N=25 kg/10a in Seokwang, N$\geq$20 kg/10a in Dongjin. In Dongjin, the rate of ripened grains was decreased with increase in nitrogen fertilizing levels and that caused to decrease the yield.

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Evaluation of the Removal Performance of Nitrogen Oxides of Foam Composites Using Activated Carbon and Titanium Dioxide (활성탄소와 이산화 타이타늄을 활용한 폼 복합체의 질소산화물의 제거 성능 평가)

  • Choi, Hyun-Chul;Choi, Young-Cheol
    • Journal of the Korea institute for structural maintenance and inspection
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    • v.26 no.6
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    • pp.120-127
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    • 2022
  • Nitrogen oxide(NOx) is a major cause of air pollution, exists in the form of nitrogen monoxide and nitrogen dioxide, and is harmful to the human body. Recently, a number of studies to reduce NOx in the atmosphere have been conducted, and these efforts have been the same in the field of construction materials. It is known that NOx can be efficiently removed by using a photocatalytic reaction. In this study, the NOx removal performance of the foam composite using activated carbon(AC) and titanium dioxide(TiO2) was investigated. AC was used to enhance the photocatalytic reaction of TiO2 by increasing the internal specific surface area of the foam composite. In this study, foam composites were prepared using the substitution rate of AC as the main variable. The NOx removal performance of specimen was evaluated according to the test method presented in ISO-22197-1. The specific surface area of the foam composite showed a tendency to increase according to the AC content, but decreased at 15% or more. Also, when the AC substitution rate was 15%, the NOx removal efficiency was the highest.

FATE AND METABOLISM OF NITROGEN IN GOATS FED 15N-LABELLED RICE STRAW

  • Xing, T.X.;He, D.Y.;He, L.H.;Zhou, W.J.;Liao, X.L.;Chen, H.P.
    • Asian-Australasian Journal of Animal Sciences
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    • v.5 no.4
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    • pp.699-707
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    • 1992
  • Three goats were fed with $^{15}N$-labelled rice straw to study the characteristics of digestion, assimilation, metabolism and excretion of C and N compounds from rice straw. It was shown that the amount of $^{15}N$ assimilated into the bodies of the two slaughtered goats accounted for 38.5 and 23.6% of the total amount of $^{15}N$ ingestion in the experimental diet by each goat. The $^{15}N$ excreted via the feces and urine for the three goats accounted for 34.8, 33.2 and 33.9% of the total amount of $^{15}N$ ingested in the feed of the 3 goats. The recoveries of $^{15}N$ from the two slaughtered goats were 73.3 and 57.5%, with the corresponding rates of $^{15}N$ loss 26.7 and 42.5% respectively. The amino acids digestibilities were 68.7 and 54.0%, while the digestibilities of carbohydrates were 74.8 and 67.7% respectively for goats 1 and 3.

Correlation of Growth Performance with Total Nitrogen, Carbon and Nitrogen Isotope Compositions in the Xylem of Pinus koraiensis (잣나무 생장과 목질부내의 전질소, 탄소 및 질소 동위원소 조성과의 상관관계)

  • Lee, Wi-Young;Park, Eung-Jun;Han, Sang-Urk
    • Journal of Korean Society of Forest Science
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    • v.99 no.3
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    • pp.353-358
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    • 2010
  • In this study, we investigated the relationship of tree-ring growth with total nitrogen content, and stable carbon and nitrogen isotopes from the core samples of Pinus koraiensis (35-year-old). Annual ring width showed significant positive correlations with the ${\delta}^{13}C$ (P=0.003). The total nitrogen content (P=0.024), and the ${\delta}^{13}C$ content was also correlated with total nitrogen content (P=0.038), indicating that the growth of P. koraiensis was stimulated as the contents of both ${\delta}^{13}C$ and total nitrogen were increased. On the other hand, the less the ${\delta}^{15}N$ content and the C/N ratio were, the larger the annual ring width was. Moreover the families with relatively better growth performance contained the higher levels of ${\delta}^{13}C$ in the xylem compared to other families. These results suggest that the ${\delta}^{13}C$ and total nitrogen contents are the important determinants in the growth performance of P. koraiensis.

Studies on the Nitrogen Economy and Primary Production of a Helianthus annuus Population (해바라기 군락의 일차생산과 질소경제)

  • 송승달
    • Journal of Plant Biology
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    • v.18 no.3
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    • pp.101-108
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    • 1975
  • The nitrogen economy and primary production of a Helianthus annuus "Manchurian" population were studied with special reference to the pattern of seasonal changes of vertical distributions of dry matter and nitrogen quantities, and its quantitative significance was discussed in relation to the pattern of the plant population growth, distribution ratios among organs, and turnover rates of dry matter and nitrogen. The population was established in plant density of 11.1plant/$m^2$ at the experimdntal field of Kyungpook National University, Daegu. During the period of population developemnt (April-September, 1973), the annual inflow rates and outflow rates of dry matter and nitrogen were 5560 gDM/$m^2$/year and 89 gN/$m^2$/year, respectively. The distribution ratios of dry matter and nitrogen to leaves were 28% and 45%, to stems 48% and 18%, to roots 13% and 5%, and to flowers and seeds 11% and 32%, respectively. The maximum turnover rates of inflow of dry matter and nitrogen were attained in May-June, and were 216%/month and 210%/month, respectively. The amount of nitrogen demand was 52gN/$m^2$/year (58%) for the foliage growth, 13 gN/$m^2$/year(15%) for the stem growth, 20 gN/$m^2$/year(23%) for the reproductive organs, and 4 gN/$m^2$/year(4%) for the growth of the underground parts. The amount of nitrogen supply by the nitrogen withdrawn from senescing leaves and stems was 25gN/$m^2$/year(28%) and the amount of nitrogen absorption by the root from the environmental soil was 64 gN/$m^2$/year(72%). The ratiio of the a mount of produced dry matter to that of assimilated nitrogen during a year was calculated for this annual plant population as 60, which can be used as the nitrogen utility index.ity index.

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Nitrogen Isotope Labeled Tetraheme Cytochrome c3 on a Defined Medium

  • Kim, Andre;Park, Jang-Su
    • Bulletin of the Korean Chemical Society
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    • v.26 no.2
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    • pp.278-280
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    • 2005
  • To obtain cytochrome $c_3$ labeled with a stable isotope, the conditions of cultivation and the composition of medium for DvMF were examined. The growth of DvMF was steady and reproducible under purging with $N_2$ and under pH control. DvMF was able to go on a defined medium without natural products. The composition of medium containing a small amount of $NH_4C$l as sole nitrogen source was established. Then, uniformly $^{15}N$labeled cytochrome $c_3$ was obtained during the culture of DvMF in a defined medium with $^{15}NH_4$Cl; it was confirmed by $^1H-^{15}N$ HMQC.

Stable Isotope Labeled Cytochrome $c_3$ from Desulfovibrio vulgaris on a Defined Medium as Sole Nitrogen Source

  • Kim, Andre;Shim, Yoon-Bo;Kang, Shin-Won;Park, Jang-Su
    • BMB Reports
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    • v.33 no.6
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    • pp.506-509
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    • 2000
  • To obtain Cytochrome $c_3$ labeled with a stable isotope, the conditions of cultivation and the composition of medium for DvMF were examined. The growth of DvMF was steady and reproducible under purging with $N_2$ and under pH control. DvMF was able to go on a defined medium without natural products. The composition of the medium containing a small amount of $NH_4Cl$ as sole nitrogen source was established. Then, uniformly $^{15}N-labeled$ Cytochrome $c_3$ was obtained during the culture of DvMF in a defined medium with $^{15}NH_4Cl$; it was confirmed by $^{1}H-^{15}N$ HMQC.

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Removal of #NH_3-N$ by using Immobilized Nitrifier Consortium in Polyvinyl Alcohol (PVA에 고정화된 질화세균에 의한 암모니아성 질소제거)

  • 서근학;김용하;조진구;김병진;서재관;박은주;김성구
    • Journal of Environmental Science International
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    • v.8 no.4
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    • pp.479-483
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    • 1999
  • Nitrifier consortium immobilized in polyvinyl alcohol was used for the removal of ammonia nitrogen from synthetic aquaculture water in the airlift bioreactor. At the aeration rate fo 0.15 vvm and bead packing volume fraction of 20%, airlift bioreactor was operated effectively for a removal of ammonia nitrogen and for a stability of operation. Ammonia nitrogen removal rate by airlift bioreactor was continuously increased with decreasing hydraulic residence time. At the HRT(hydraulic residence time) of 0.3 hour, ammonia nitrogen removal rate was 84.3 g/$m^3$.d and the highest ammonia nitrogen removal rate was 130.8 g/$m^3$.d when HRT was 0.1 hour.

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Effects of Nitrogen Levels on Green Yield and Nitrate Content of Italian Ryegrass (Lolium multiflorum Lam.) (질소시비수준(窒素施肥水準)이 이탈리안라이그라스의 생초생산량 및 질산태질소(窒酸態窒素) 함량(含量)에 미치는 영향)

  • Lee, Chun-Hee;Cheon, Seong-Gun;Choi, Seung-Lhak;Lee, Han-Saeng;Shin, Weon-Kyo
    • Korean Journal of Soil Science and Fertilizer
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    • v.23 no.1
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    • pp.40-43
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    • 1990
  • Study was conducted to investigate the optimum amount of nitrogen fertilizer for the green forage production of Italian ryegrass at the paddy field after rice harvesting in southern part of Korea in 1987 and 1988. Effects of three levels of nitrogen fertilizer application (15, 30 and 45 kg N/10a) on the green yield and nitrate content of green forage at three cutting times (April 30, May 20 and June 10) were examined in 1988. The results were as follows. 1. Yield of green forage at all cutting times increased in order of 45 > 30 > 15 kg N/10a, and it increased about 14 percent in the 45 kg N/10a and decreased about 35 percent in the 15 kg N/10a compared with the 30 kg N/10a. 2. Nitrate content in the green forage at all cutting times was high with increasing amount of nitrogen fertilizer and with decreasing ratio of K/(Ca+Mg) in green forage. The nitrate content at the second and the third cutting times in the 45 kg N/10a might be outrunned the tentative limitation for cattle. 3. Maximum amount of nitrogen fertilizer was 48 kg/10a for total green forage production, but it was 38 kg/10a near at cattle's harmful limit of nitrate content in green forage.

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