BMB Reports

Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Stable Isotope Labeled Cytochrome $c_3$ from Desulfovibrio vulgaris on a Defined Medium as Sole Nitrogen Source

  • Kim, Andre (Department of Chemistry and Chemistry Institute for Functional Materials, Pusan National University) ;
  • Shim, Yoon-Bo (Department of Chemistry and Chemistry Institute for Functional Materials, Pusan National University) ;
  • Kang, Shin-Won (Department of Chemistry and Chemistry Institute for Functional Materials, Pusan National University) ;
  • Park, Jang-Su (Department of Chemistry and Chemistry Institute for Functional Materials, Pusan National University)
  • Received : 2000.10.30
  • Accepted : 2000.11.10
  • Published : 2000.11.30
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Abstract

To obtain Cytochrome $c_3$ labeled with a stable isotope, the conditions of cultivation and the composition of medium for DvMF were examined. The growth of DvMF was steady and reproducible under purging with $N_2$ and under pH control. DvMF was able to go on a defined medium without natural products. The composition of the medium containing a small amount of $NH_4Cl$ as sole nitrogen source was established. Then, uniformly $^{15}N-labeled$ Cytochrome $c_3$ was obtained during the culture of DvMF in a defined medium with $^{15}NH_4Cl$; it was confirmed by $^{1}H-^{15}N$ HMQC.

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