• Molecules and Cells
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• 제40권1호
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• pp.17-23
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• 2017

Mitogen-activated protein kinase (MAPK) signaling cascades play critical roles in various cellular events in plants, including stress responses, innate immunity, hormone signaling, and cell specificity. MAPK-mediated stress signaling is also known to negatively regulate nitrogen-fixing symbiotic interactions, but the molecular mechanism of the MAPK signaling cascades underlying the symbiotic nodule development remains largely unknown. We show that the MtMKK5-MtMPK3/6 signaling module negatively regulates the early symbiotic nodule formation, probably upstream of ERN1 (ERF Required for Nodulation 1) and NSP1 (Nod factor Signaling Pathway 1) in Medicago truncatula. The overexpression of MtMKK5 stimulated stress and defense signaling pathways but also reduced nodule formation in M. truncatula roots. Conversely, a MAPK specific inhibitor, U0126, enhanced nodule formation and the expression of an early nodulation marker gene, MtNIN. We found that MtMKK5 directly activates MtMPK3/6 by phosphorylating the TEY motif within the activation loop and that the MtMPK3/6 proteins physically interact with the early nodulation-related transcription factors ERN1 and NSP1. These data suggest that the stress signaling-mediated MtMKK5/MtMPK3/6 module suppresses symbiotic nodule development via the action of early nodulation transcription factors.

• Animal Bioscience
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• 제37권3호
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• pp.522-535
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• 2024

Objective: Transition period is considered from 3 weeks prepartum to 3 weeks postpartum, characterized with dramatic events (endocrine, metabolic, and physiological) leading to occurrence of production diseases (negative energy balance/ketosis, milk fever etc). The objectives of our study were to analyze the periodic concentration of serum beta-hydroxy butyric acid (BHBA), glucose and oxidative markers along with identification, and validation of the putative markers of negative energy balance in buffaloes using in-silico and quantitative real time-polymerase chain reaction (qRT-PCR) assay. Methods: Out of 20 potential markers of ketosis identified by in-silico analysis, two were selected and analyzed by qRT-PCR technique (upregulated; acetyl serotonin o-methyl transferase like and down regulated; guanylate cyclase activator 1B). Additional two sets of genes (carnitine palmotyl transferase A; upregulated and Insulin growth factor; downregulated) that have a role of hepatic fatty acid oxidation to maintain energy demands via gluconeogenesis were also validated. Extracted cDNA (complementary deoxyribonucleic acid) from the blood of the buffaloes were used for validation of selected genes via qRTPCR. Concentrations of BHBA, glucose and oxidative stress markers were identified with their respective optimized protocols. Results: The analysis of qRT-PCR gave similar trends as shown by in-silico analysis throughout the transition period. Significant changes (p<0.05) in the levels of BHBA, glucose and oxidative stress markers throughout this period were observed. This study provides validation from in-silico and qRT-PCR assays for potential markers to be used for earliest diagnosis of negative energy balance in buffaloes. Conclusion: Apart from conventional diagnostic methods, this study improves the understanding of putative biomarkers at the molecular level which helps to unfold their role in normal immune function, fat synthesis/metabolism and oxidative stress pathways. Therefore, provides an opportunity to discover more accurate and sensitive diagnostic aids.

• Journal of the Korean Academy of Child and Adolescent Psychiatry
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• 제7권2호
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• pp.203-212
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• 1996

주의력결핍 과잉행동장애아(ADHD)와 품행장애나 반항성장애와 같은 외면성 증후를 공존질환으로 갖는 주의력결핍 과잉행동장애아(ADHD + CD/ODD)간에 사회경제적지위, 부모 학력수준, 생활 스트레스 사건, 친인척의 정신병력을 내용으로 하는 가족 배경과 부모의 결혼 만족도, 양육자 스트레스, 양육태도 등의 심리사회적 변인에 차이가 있는지 검증하였다. 연구결과, ADHD+CD/ODD집단이 위의 가족배경에서 가장 열악한 수준을 보이고 있었고, 부모의 결혼적응도에서도 ADHD+CD/ODD 집단이 가장 낮았으며, 양육자 스트레스에서는 ADHD+CD/ODD>ADHD>통제집단 순으로 높았으며 부모가 지각한 양육태도에서 임상집단의 어머니들이 증오적, 거부적 의존성을 포함하는 양육태도를 가지고 있었으며, 아동이 지각한 부모의 양육태도에서는 임상집단의 부모가 아동에게 더 거부적이고 지배적이고, 모순적인 양육태도를 지닌 경향성을 알 수 있었다.

• 생물정신의학
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• 제24권1호
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• pp.10-18
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• 2017

Occupational hazards of firefighting and rescue works include frequent exposure to emergencies and life-threatening situations. These stressful work conditions of being constantly under pressure and exposed to potentially traumatic events put them at higher risk of developing posttraumatic stress disorder (PTSD), compared to the general population. PTSD is a potentially debilitating mental disorder, due to persistent intrusive thoughts, negative alterations of mood and cognition, hypervigilance, avoidance of similar situations and reminders, and re-experiences of the traumatic event. Previous studies have shown a relatively high prevalence of PTSD among firefighters, indicating the need for a systematic approach of early detection and prevention. Therefore, a critical review of the current literature on PTSD in firefighters would provide valuable insights into developing effective prevention and intervention programs. Literature indicated that there are risk factors of PTSD in firefighters, such as pre-existing depression, anxiety, sleep disorders, occupational stress, physical symptoms, and binge drinking, whereas social support and adequate rewards are protective factors. Although there are differences in the prevalence of PTSD across studies, partly due to various assessment tools utilized, different sample sizes, and sample characteristics, over one tenth of the firefighters were estimated to have PTSD. The current review warrants further investigations to precisely assess PTSD and co-morbid mental disorders, functional outcomes, and associated factors, and to develop evidence-based preventive and interventional programs to help firefighters with PTSD.

• 성인간호학회지
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• 제24권2호
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• pp.171-182
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• 2012

Purpose: This study aimed to identify depression and its predictors among Korean community-dwelling elderly with mild cognitive impairment. Methods: Secondary data analyses of the data collected by the "Study on tailored integration program for reinforcing cognitive and physical function of the frail elderly. The study used data from one-to-one interviews using structured questionnaires. The subjects were 346 community-dwelling elderly who visited a healthcare center in Seoul, Korea. A descriptive correlational study design was utilized to explore depression and its predictors including physical factors and psychosocial factors among the elderly with mild cognitive impairment. Data were analyzed using t-test, Pearson's correlation, and hierarchical regression. Results: Mean score of depression was within normal limit $11.61{\pm}6.69$. Somatic symptoms, negative life events (severity), social support were shown as significant predictors of depression. The most influential predictor for depression was somatic symptoms (${\beta}$=.340, $p$ <.001). Conclusion: To prevent and relieve depression in the elderly with mild cognitive impairment, nursing intervention strategies which consider well-balanced physical and psychosocial aspects are needed. In particular, a specific nursing intervention strategy is required to improve physical health of the elderly with mild cognitive impairment.

• Journal of the Korean Academy of Child and Adolescent Psychiatry
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• 제26권3호
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• pp.149-158
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• 2015

Objectives : The objective of this study was to identify the risky behavior subtypes in adolescents with depression, and the relationship between those subtypes and suicide attempt. Methods : Ninety adolescents who met the Diagnostic and Statistical Manual of Mental Disorders-4th edition criteria for major depressive disorder, dysthymia, or depressive disorder not otherwise specified participated in the study. Participants were interviewed about suicidal attempts, non-suicidal self-injuries. Smoking, drinking behaviors, and searching for the word "suicide" on the internet, negative life events including bulling-bullied experiences, and history of abuse were also included with questionnaires. Results : The results of cluster analysis showed that four risky behaviors were divided into three clusters : non-risky type, characterized by few risky behaviors ; delinquency type, which was characterized by smoking and drinking ; non-suicidal self-injury type. In non-suicidal self-injury type, percentage of girls was higher and clinical symptoms were more severe than delinquency type. In addition, delinquency type members could be a bully, while non-suicidal self-injury type members could be abused by a family member. Logistic regression analysis showed that both risky behavior subtypes were significant predictors of suicide attempt. Conclusion : These results suggest that two types of risky behaviors of depressed adolescents have an effect on the suicide attempts independently. Then, the implications and limitations of the study were discussed and directions for future studies were suggested.

• 생물치료정신의학
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• 제24권3호
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• pp.129-141
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• 2018

Suicide is a behavior that is intended to cause death by itself and requires medical treatment, resulting in suicidal attempt or completion. Suicide causes loss of life, damages the body, costs a lot of medical expenses, and causes families to fall into sorrow and suffering therefore this suicide is a huge loss to family and society. There have been attempts to reduce and prevent suicide by understanding the mechanism of suicide. The mechanism of suicide can be thought of as psychological mechanism and biological mechanism. In the past, if we considered the psychological and biological mechanisms separately, the development of neuroscience now connects and integrates these two. Psychological factors affect biological factors and biological temperaments also affect perception or thinking about the situation and increase psychological vulnerability. Distant factors in suicidal behavior-such as childhood adversity and family and genetic predisposition-increase the lifetime risk of suicide. They alter the response to stress and other processes through changes in gene expression and regulation of emotional and behavioral characteristics. Distant factors affect the biological system and consequently changes in these systems can increase the risk of suicide. In other words, the distal factor does not directly induce suicidal behavior but rather acts indirectly through developmental or mediating factors. These mediating factors are impulsive aggressive and anxious trait, and chronic use of substances. The mechanism of this disorder is the abnormality of the serotonin system and the abnormality of the lipid level. Proximal factors are associated with the onset of suicide events and include changes in the major neurotransmitter systems, inflammatory changes, and dysfunction of glial cells in the brain. A series of studies, including a variety of research methods and postmortem and in-vivo imaging studies, show the impairment of the serotonergic neurotransmitter system and hypothalamic-pituitary-adrenal axis stress response system for suicidal behavior. These disorders lead to suicidal behavior due to difficulty in cognitive control of mood, pessimism, reactive aggression, abnormality in problem solving abilities, excessive response to negative social signals, severe emotional distress, and cognitive dysregulation of suicidal ideation.

• 한국융합학회논문지
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• 제12권2호
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• pp.233-246
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• 2021

본 연구에서는 외상 유형(단순 외상과 복합 외상)에 따라 PTSD 증상의 심각도와 자기 및 타인개념에 차이가 있는지를 확인하고자 하였다. 총 166명의 대학생 및 대학원생들 중 단순 외상(n=31)과 복합 외상(n=30)으로 분류된 참여자들의 자료를 최종 분석에 포함하였다. 참여자들은 생활사건 체크리스트(LEC), 개정된 사건 충격 척도(IES-R)와 E-prime으로 제작된 정서 스트룹 과제를 수행하였다. 결과는 다음과 같다. 첫째, 단순 외상 집단보다 복합 외상 집단에서 더 높은 수준의 PTSD 증상이 나타났다. 둘째, 스트룹 과제에서 부정적인 자기개념을 반영하는 단어를 2초 제시한 조건에서 복합 외상 집단이 단순 외상 집단보다 반응시간이 더 길었다. 이러한 결과는 복합 외상 집단과 단순 외상 집단이 적어도 PTSD 증상 및 자기개념에 다른 특성을 가지고 있다는 것을 제안한다. 마지막으로, 본 연구의 의의와 한계점에 대해 논의하였다.

• 한국식생활문화학회지
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• 제22권6호
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• pp.783-793
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• 2007

The purpose of this research was to investigate consumers' awareness and perception of the noble family food to popularize it. A total of 1,100 subjects lived in Seoul area participated in Web-based survey. Thirty eight percents of the participants were not even aware that there exists the noble family food and overall 63% of the participants merely have the idea of what the noble family food is. The impressions of the noble family food varied widely. Some of them were positive as the noble family food seems good for health and some were negative as it is not easy to cook. The results showed that many people are not interested in noble family food, however, there was a high behavioral intention to have it in their future meal plan. Participants responded that noble family food had not fully developed yet (mean=4.08), but it had potential to be world-wide excellent (mean=3.95). There was significantly high scored response that the noble family food should contain sanitary cooking process to popularize it (mean=4.16). Also participants addressed that it was necessary to use public relations through mass media (mean=4.02), and it required appropriate educational approach to the noble family food (mean=4.02). In addition, people perceived that traditional custom and the noble family foods were jointly connected by cultural events. On the other hand, there were low scored responses on developing of noble family food as a processed food to be generally accessible in daily life. In conclusion, the importance of public relations should be emphasized to popularize the noble family food. Also, increasing the number of places that sell noble family food would help to popularize it.

• 산업진흥연구
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• 제6권2호
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• pp.37-46
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• 2021

본 연구는 아동·청소년기 근친 성폭력 피해자들이 사건 이후 겪게 되는 다양한 심리적 변화와 현실을 그들의 목소리로 전달하여 새로운 시각을 제공해야 할 필요성과 상담을 통해 삶에 적응하기 위해 노력하는 개인적인 삶의 경험을 현상학적인 접근을 통해서 이해하고 기록하였다. 현상학적 방법론을 통한 분석 결과는 다음과 같다. 첫째, 참여자들은 부모의 정서적인 방치와 가족구성원의 폭력적 행동과 위협, 침묵을 강요하는 과정을 통해 대인관계에 대한 불신감과 낮은 자존감이 형성 되었고 일상생활에서 지속적인 어려움을 겪게 되었다. 둘째, 성폭력 사건에 의한 고통과 후유증은 사건이 알려지는 것에 대한 두려움과 자신에 대한 부정적인 생각, 대인관계의 어려움으로 나타나 현실부적응과 사회활동 회피를 반복하면서 역할의 갈등을 경험하게 하였다. 셋째, 아동·청소년기 근친 성폭력의 후유증은 PTSD를 통해 극단적으로 드러났다. PTSD의 경험은 참여자가 정신과에서 처방받은 약물이나 알코올과 같은 물질에 의존하고 환청과 환각, 망상의 경험으로 외부활동을 더욱 기피하게 하는 요소가 되었다. 넷째, 적응 과정을 통한 변화는 사건에 대한 직면과 분리, 삶에 대한 새로운 시선이다. 적응 프로그램을 진행하면서 과거의 상처로 인해 표현하지 못한 말과 감정을 표현하고, 자신의 삶을 살아가기 위한 목표를 세우고 앞으로 나아가려고 했다. 현실에서의 극복 경험은 참여자에게 자기존중감과 삶에 대한 자신의 능력을 신뢰하는 자기효능감, 건강한 자기조절능력에 대한 믿음을 향상시켰다. 본 연구는 개인의 삶에서 근친 성폭력 트라우마가 새로운 일상의 위기를 통해 재경험되고 각 과정마다 새로운 적응 과정을 반복하는 모델을 제시하는데 의의를 둔다.