• Applied Biological Chemistry
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• v.38 no.1
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• pp.49-54
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• 1995

To understand the molecular structure and pathogenesis mechanism of Korean garlic viruses, we have isolated cDNA clones for garlic viruses. The partial nucleotide sequences of 24 cDNA clones were determined and those of five clones containing poly(A) tail were compared with sequences of other plant viruses. One of these clones, V9, has a primary structure similar to the carlavirus group, suggesting that the clone V9 derived from a part of garlic latent virus (GLV). Northern blot analysis with the clone V9 as a probe demonstrated that GLV genome is 8.5 knt long and has a poly(A) tail. The clone V9 encodes coat protein (CP) of 33 kDa and nucleic acid binding protein of 10 kDa in different reading frame. The hexanucleotide motif, 5'-ACCUAA, which is conserved in the 3' noncoding region arid was proposed to be a cis-acting element involved in the production of negative strand genomic RNA was noticed. Complementary sequence to the hexanucleotide motif, 5'-TTAGGT, is also found in the positive strand of V9 RNA. The putative CP gene was cloned into the pRSET-A expression vector and expressed in E. coli BL21. The expressed recombinant V9CP protein was purified by $Ni^{2+}$ NTA affinity chromatography. The anti-V9CP antibody recognizes 34 kDa polypeptide which could be CP of GLV in infected garlic leaf extract. Immunoblot and Northern blot analysis of various cultivars shows wide occurrence of GLV in Korean garlic plants.

• Journal of the Korean Geosynthetics Society
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• v.19 no.4
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• pp.21-32
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• 2020

As the global large-scale infrastructure construction market expands, the construction of civil engineering structures in extreme environments such as cold or hot regions is being planned or constructed. Accordingly, the construction of the pile foundation is essential to secure the bearing capacity of the upper structure, but there is a concern about loss of stability and function of the pile foundation due to the possibility of ground deformation in extreme cold and hot regions. Therefore, in this study, a new type of pile foundation is developed to respond with the deformation of the ground, and the ground deformation that can occur in extreme cold and hot region is largely divided into heaving and settlement. The new type of pile foundation is a form in which a cylinder capable of shrinkage and expansion is inserted inside the steel pipe pile, and the effect of the cylinder during the heaving and settlement process was analyzed numerically. As a result of the numerical analysis, the ground heaving caused excessive tensile stress of the pile, and the expansion condition of the cylinder shared the tensile stress acting on the pile and reduced the axial stress acting on the pile. Ground settlement increased the compressive stress of the pile due to the occurrence of negative skin friction. The cylinder must be positioned below the neutral point and behave in shrinkage for optimum efficiency. However, the amount and location of shrinkage and expansion of cylinder must comply with the allowable displacement range of the upper structure. It is judged that the design needs to be considered.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.29 no.5B
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• pp.473-482
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• 2009

This study numerically discusses wave forces acting on a vertical wall such as breakwaters or revetments, subjected to incident undular or turbulent bores. Due to the complex hydrodynamics of bore, its wave forces have been predicted, mainly through laboratory experiments. Numerical simulations in this paper were carried out by CADMAS-SURF(CDIT, 2001), which is based on Navier-Stokes momentum equations and VOF method (Hirt and Nichols, 1981) for tracking free water surface. Its original source code was also partly revised to generate bore in the numerical water channel. Numerical raw data computed by CADMAS-SURF included great strong spike phenomena that show the abrupt jumps of wave loads. To resolve this undesired noise of raw data, the band-pass filter with the frequency of 5Hz was utilized. The filtered results showed reasonable agreements with the experimental results performed by Matsutomi (1991) and Ramsden (1996). It was confirmed that CADMASSURF can be applied to the design of coastal structures against tsunami bores. In addition, the transformation process and propagation speed of bores in the same 2-d water channel were discussed by the variations of water level for time and space. The numerical results indicated that the propagation speed of bore was changed due to the nonlinear interactions between negative and reflected waves.

• Journal of Biosystems Engineering
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• v.2 no.1
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• pp.33-48
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• 1977

Transporting agricultural products and the other material by the two-wheel-tractor (power-tiler)and trailer system may be one of its most widely used farming functions.The safety and hitching load for all the previaling performing conditions may be the general concern over the operation of the tiller-trailer system. In this study, a mathematical model to determine the static and dynamic forces excerting on the hitch point were developed . Based on the analysis of the model and the field measurements. the limiting hitching load and critical slope were analyzed. The results of the study are summarized as follows ; 1) The limit angle of slope land for the safety steering that two-wheel tractor-single axle trailer system was able to transport agricultural products was the direct angle (${\gamma}$) = 8 ; the cross angle$\beta$) 15 ; and it was decreased in accordance with the increase of carrying load ($W_4). 2) The critical velocity for safe operation in case of running on downward hill road was about 1.08m/sec. 3) The limiting carrying load for the safe steering was W$_4$=600kg. The degree of the safe steering for different braking methods was given in order as follows ; Simulataneous braking the tractor and trailer , braking the trailer only, and braking tractor only. 4) Among the three components of impact loads excerting on the hitch point, the component in the lateral direction ($P_{Vy}$) was near zero in spite of increase of hitching load ($W_4) , while the components in the other two mutually perpedicular directions ($P_{Vx}$ and ($P_{Vz}$) ) had larger values in horizontal plane than those in the slope lands. 5) Moment of forces on the lateral direction (M$y$) had the largest value among the three components of impact moment acting on the hitch point, however all the components were sharply increased in accordance with the increase of hitching loads ($W_4. Three components of the moment were the negative values.

• Journal of Microbiology
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• v.44 no.1
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• pp.35-41
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• 2006

The unique gene encoding thioredoxin reductase (TrxR) was previously cloned and characterized from the fission yeast Schizosaccharomyces pombe, and its expression was induced by oxidative stress. To elucidate tbe regulatory mechanism of the S. pombe TrxR gene, three fusion plasmids were generated using polymerase chain reaction: pYUTR20, pYUTR30, and pYUTR40. Plasmid pYUTR20 has an upstream region of 891 base pairs, pYUTR30 has 499 in this region, and pYUTR40 has an 186 bp upstream region. Negatively acting sequence is located between $-1,526\;\~\;-891bp$ upstream of the gene. The upstream sequence, responsible for the induction of TrxR by menadione (MD), is situated on the $-499\;\~\;-186bp$ region, which is also required for TrxR induction by mercuric chloride. The same region also appeared to be required for Pap1-mediated transcriptional regulation of the TrxR gene, which contains the two plausible Papl binding sites, TTACGAAT and TTACGCGA. Consistently, basal and inducible expression of the TrxR gene was markedly lower in the Pap1-negative TP108-3C cells than in wild-type yeast cells. In summary, up-regulation of the S. pombe TrxR gene is mediated by Pap1 via the transcriptional motif(s) located on the $-499\;\~\;-186bp$ region.

• Archives of Plastic Surgery
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• v.45 no.6
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• pp.504-511
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• 2018

Background Acellular dermal matrix (ADM) helps wound healing by stimulating angiogenesis, acting as a chemoattractant for endothelial cells, providing growth factors, and permitting a substrate for fibroblasts to attach. The current standard for using paste-type ADM (CG Paste) in wound healing is direct application over the wounds. The major concerns regarding this method are unpredictable separation from the wounds and absorption into negative-pressure wound therapy devices. This study aimed to investigate the effects of subcutaneous injection of paste-type ADM on wound healing in rats. Methods Full-thickness skin defects were created on the dorsal skin of rats. Eighteen rats were randomly divided into three groups and treated using different wound coverage methods: group A, with a saline dressing; group B, standard application of CG Paste; and group C, injection of CG Paste. On postoperative days 3, 5, 7, 10, and 14, the wound areas were analyzed morphologically. Histological and immunohistochemical tissue analyses were performed on postoperative days 3 and 7. Results Groups B and C had significantly less raw surface than group A on postoperative days 10 and 14. Collagen fiber deposition and microvessel density were significantly higher in group C than in groups A and B on postoperative days 3 and 7. Conclusions This study showed comparable effectiveness between subcutaneous injection and the conventional dressing method of paste-type ADM. Moreover, the injection of CG Paste led to improved wound healing quality through the accumulation of collagen fibers and an increase in microvessel density.

• Proceedings of the SCSK Conference
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• 2003.09a
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• pp.387-412
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• 2003

Influence of gelatinase on basement membrane (BM) structure was investigated by using a skin equivalent (SE) model. The results showed that (1) gelatinase produced by cells degraded the BM and (2) the addition of matrix metalloproteinase-specific inhibitor to the SE medium accelerated the formation of BM structure, indicating that gelatinase is involved in BM impairment. The activity of gelatinase was also studied in healthy human facial skin tissues. The result of in situ zymography revealed gelatinase activity around the basal layer of the epidermis, where BM integrity was severely compromised. Therefore, this enzyme was suggested to be associated with BM decomposition in human facial skin. To assess the behavior of gelatinase in stratum corneum (SC) non-invasively, an immunological study was performed. Since positive immunostaining of pro-gelatinase B was observed in SC stripped from sun-exposed skin, whereas no positive staining detected in SC of non-irradiated skin, gelatinase in the epidermis could be non-invasively detected by measuring gelatinase in SC. Gelatinase in SC of healthy female volunteers was monitored using a special film that sensitively and conveniently detects gelatinase. Ninetr percent of SC from facial skin (l00 women, 40's-50's) was gelatinase-positive. On the other hand, SC from non-irradiated skin was negative. These results strongly suggest that (1) gelatinase is constantly produced in the facial epidermis of most middle-aged woman during their daily life, and (2) the enzyme might be involved in the aging-related degeneration of both BM and the matrix fibers of the upper layer of the dermis, acting as a very important aging factor. Strong inhibitory activity against gelatinase was found in turmeric extract and identified curcumin as the major ingredient. Topical application of cream containing turmeric extract significantly decreased the number of gelatinase-positive SC clusters in human facial skins. These results indicated that turmeric is an effective ingredient to prevent skin from photo aging by suppressing chlonically upregulated gelatinase activity by UV and to improve skin condition.

• Proceedings of the KWS Conference
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• 2002.10a
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• pp.757-762
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• 2002

Many reports have been shown that the buoyancy, electromagnetic force, surface tension, and gas shear stress are the driving forces of weld pool circulation in arc welding. Among them, the surface tension of molten metal plays an important role in the flow in weld pool, which are clarified by the specially designed experiments with small particles as well as the numerical simulations. The surface tension is also related to the penetration in arc welding. Therefore, a quantitative evaluation of surface tension is demanded for the development of materials and arc process control. However, there are few available data published on the surface tension of molten metals, since it depends on the temperature and the composition of materials. In this study, a new method was proposed for the evaluation of surface tension and its temperature dependence, in which it is evaluated by the equilibrium condition of acting forces under a given surface geometry, especially back surface. When this method was applied to the water pool and to the back surface of molten pool in the stationary gas tungsten arc welding of thin plate, following results were obtained. In the evaluation of surface tension of water, it was shown that the back surface geometry was very sensitive to the evaluation of surface tension and the evaluated value coincided with the surface tension of water. In the measurement of molten pool in the stationary gas tungsten arc welding, it was also shown that the comparison between the surface tension and temperature distribution across the back surface gave the temperature dependent surface tension. Applying this method to the mild steel and stainless steel plates, the surface tension with negative gradient for temperature is obtained. The evaluated values are well matched with ones in the published papers.

• The Journal of the Korea Contents Association
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• v.16 no.7
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• pp.504-512
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• 2016

This study was performed to explore the institutional treatment system by analyzing the pattern of musical actor's projection and aftereffect subsequent to the completion of performance. In-depth interviews were conducted with 22 top-class musical actors and actresses of the Republic of Korea. The result of the survey indicated that most actors and actresses suffer from projection and it's aftereffect such as depression for more than one month after performance, and that they tend to rely on self-healing based on self-diagnosis rather than seeking for professional counselling. The symptoms of projection were shown more strongly when the actors or actresses were taking on the role of main character, or acting as a negative or realistic character, or when expressing strong and extreme emotions. In this era of Korean culture, the result of this study implies that our country have to establish the actor's mental caring-related new law and private care centers supported enough funds to encourage the actors to receive more effective actor-friendly treatment service before and after the performance.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.15 no.3
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• pp.1311-1317
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• 2014

This is a descriptive study that attempts to provide basic information for easing job strain and enhancing the efficiency of cosmetology industry workers by examining the effects that the emotional labor and stress-coping of cosmetology workers have on their job strain. As a result of a multiple regression analysis of the factors affecting the subjects job strain, their surface acting and problem-focused coping, unconcern coping, social support were seen as factors that accounted for 30% of their job strain. Of the emotional labor of the subjects, the external behaviors displayed positive correlations with job strain; and they showed negative correlations with problem-focused coping and social support. Concerning emotional labor, sincere behavior was found to have positive correlations with problem-focused coping, hoping, social support, positive coping and strain relieving. Based on the results of this study, it is deemed that, along with interventions for emotional labor, education and intervention programs for a healthy and positive stress coping are needed in regards to interventions for cosmetology professionals.