Journal of Microbiology

The Microbiological Society of Korea (한국미생물학회)
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Transcriptional Analysis and Pap1-Dependence of the Unique Gene Encoding Thioredoxin Reductase from the Fission Yeast

  • Kang Hyun-Jung (Division of Life Sciences, College of Natural Sciences, Kangwon National University) ;
  • Hong Sung-Min (Division of Life Sciences, College of Natural Sciences, Kangwon National University) ;
  • Kim Byung-Chul (Division of Life Sciences, College of Natural Sciences, Kangwon National University) ;
  • Kim Kyunghoon (Division of Life Sciences, College of Natural Sciences, Kangwon National University) ;
  • Park Eun-Hee (College of Pharmacy, Sookmyung Women's University) ;
  • Lim Chang-Jin (Division of Life Sciences, College of Natural Sciences, Kangwon National University)
  • Published : 2006.02.01
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Abstract

The unique gene encoding thioredoxin reductase (TrxR) was previously cloned and characterized from the fission yeast Schizosaccharomyces pombe, and its expression was induced by oxidative stress. To elucidate tbe regulatory mechanism of the S. pombe TrxR gene, three fusion plasmids were generated using polymerase chain reaction: pYUTR20, pYUTR30, and pYUTR40. Plasmid pYUTR20 has an upstream region of 891 base pairs, pYUTR30 has 499 in this region, and pYUTR40 has an 186 bp upstream region. Negatively acting sequence is located between $-1,526\;\~\;-891bp$ upstream of the gene. The upstream sequence, responsible for the induction of TrxR by menadione (MD), is situated on the $-499\;\~\;-186bp$ region, which is also required for TrxR induction by mercuric chloride. The same region also appeared to be required for Pap1-mediated transcriptional regulation of the TrxR gene, which contains the two plausible Papl binding sites, TTACGAAT and TTACGCGA. Consistently, basal and inducible expression of the TrxR gene was markedly lower in the Pap1-negative TP108-3C cells than in wild-type yeast cells. In summary, up-regulation of the S. pombe TrxR gene is mediated by Pap1 via the transcriptional motif(s) located on the $-499\;\~\;-186bp$ region.

Keywords

References

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