• Title/Summary/Keyword: Natural Product

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Chinoketides A and B, Two New Antimicrobial Polyketides from the Endophytes of Distylium chinense with the "Black-Box" Co-culture Method

  • Lv, Meng-Meng;Tan, Ming-Hui;Lu, Li-Wen;Zhang, Rong-Hua;Guo, Zhi-Yong;Liu, Cheng-Xiong;Yang, Jin;Zou, Kun;Proksch, Peter
    • Natural Product Sciences
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    • v.24 no.3
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    • pp.159-163
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    • 2018
  • Two new polyketides, chinoketides A and B (1 - 2) with a known compound xylarphthalide A (3), were isolated from the solid medium of the endophytes from the leaves of the relic plant Distylium chinense with the "black-box" co-culture method, and the structures of two new compounds were elucidated by NMR, MS and CD spectra. And the absolute configurations of chinoketides A (1) and B (2) were determined as 2R,3R,8S and 5R,6S by calculating their ECD spectra to compare with the experimental CD spectra. Finally, the antimicrobial activities were evaluated to Erwinia carotovora sub sp. Carotovora (Jones) Bersey et al, and the results showed that compounds 1 - 3 displayed the antimicrobial activities with MIC value at 20.5, 30.4 and $10.2{\mu}g/mL$.

Chemical Constituents from the Stony Coral Alveopora japonica

  • Youn, Ui-Joung;Lee, Yoo-Jin;Jeon, Ha-Rim;Shin, Hyun-Ji;Son, Young-Min;Nam, Joo-Won;Han, Ah-Reum;Song, Jun-Im;Won, Yong-Jin;Seo, Eun-Kyoung
    • Natural Product Sciences
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    • v.17 no.1
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    • pp.1-4
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    • 2011
  • Chemical investigations of the stony coral Alveopora japonica Eguchi (Poritidae) resulted in the isolation of four known compounds (1 - 4). The structures of 1 - 4 were identified as a sterol, ergosta-5,24(28)-dien-$3{\beta}$-ol (1), a mixture of monoacyl glycrols (2), eicosanoic acid and tetracosanoic acid, and two nucleosides, thymine (3) and 2'-deoxythymidine (4), respectively, on the basis of spectroscopic and physicochemical analyses including 1D- and 2D- NMR techniques as well as by comparison of their data with the published values. Compounds 1 - 4 were isolated from this species for the first time. Moreover, these compounds (1 - 4) were found in the genus Alveopora and the family Poritidae for the first time.

Minor Phenolic Constituents of the Anemarrhenae Rhizoma

  • Youn, Ui-Joung;Lee, Ye-Seul;Jeong, Ha-Na;Nam, Joo-Won;Lee, Yoo-Jin;Son, Young-Min;Hwang, Eun-Sook;Seo, Eun-Kyoung
    • Natural Product Sciences
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    • v.15 no.4
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    • pp.203-207
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    • 2009
  • A homoisoflavanone, 7,4'-dihydroxyhomoisoflavanone (1) and a flavanone, (2S)-7,4'-dihydroxy-5-methoxyflavanone (2), were isolated from the rhizomes of Anemarrhena asphodeloides, together with 4,4'-dihydroxychalcon (3), 2'-O-methylphlorethin (4), 1,3-bis-di-p-hydroxyphenyl-4-penten-1-one (5), and 2,4'-dihydroxy-4-methoxybenzophenone (6) on the basis of spectroscopic and physicochemical analyses including 1Dand 2D- NMR techniques as well as by comparison of their data with the published values. Compounds 1 - 4 were isolated for the first time from this plant source. Among isolates, compound 2 exhibited moderate inhibitory effect on the differentiation of pre-adipocyte 3T3-L1 cells.

Rapid Identification of Radical Scavenging Compounds in Blueberry Extract by HPLC Coupled to an On-line ABTS Based Assay and HPLC-ESI/MS

  • Kim, Chul-Young;Lee, Hee-Ju;Lee, Eun-Ha;Jung, Sang-Hoon;Lee, Dong-Un;Kang, Suk-Woo;Hong, Sae-Jin;Um, Byung-Hun
    • Food Science and Biotechnology
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    • v.17 no.4
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    • pp.846-849
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    • 2008
  • This study employed high performance liquid chromatography (HPLC) coupled to an on-line $ABTS^+$ radical scavenging detection (RSD) system along with HPLC-electro spin impact/mass spectrometry (ESI/MS), to rapidly determine and identify antioxidant compounds occurring in blueberry extract. The extract was separated by HPLC, and then the radical scavenging activities of the separated compounds were evaluated by the on-line coupled $ABTS^+$-RSD system. The negative peaks of the $ABTS^+$-RSD system, which indicates the presence of antioxidant activity, were monitored by measuring the decrease in absorbance at 734 nm. The active components in the blueberry extract were identified by HPLC-ESI/MS using their MS spectra and retention times. According to the data acquired from the on-line HPLC-$ABTS^+$-based assay and HPLC-ESI/MS systems, the antioxidant compounds detected in the blueberry extract were identified as chlorogenic acid and 11 anthocyanins.

Validation and Content Analysis of Cornusiin B Isolated from Cornus alba (흰말채 나무에 함유된 Cornusiin B에 대한 Validation 및 함량 분석)

  • Park, Dong Hui;YIN, JUN;Wang, Hye Soo;Kim, Min;Kim, Min Ji;Lee, Eun kyeng;Lee, Min Won
    • Korean Journal of Pharmacognosy
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    • v.50 no.3
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    • pp.155-158
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    • 2019
  • Cornus alba (CA) geographically distributes in Korea, Japan, Northeast China and Russia. The cornus species have been used traditionally as anti-inflammatory, hemostatic and diuretic in Korea. Previously, it was reported CA and its separated components have excellent antioxidant, anti-inflammatory and anti-prostate cancer efficacy. Present work, the validation and content determination was tried on the ellagitannin, cornusiin B which were isolated from the CA by using high-performance liquid chromatography (HPLC). As a result, the content of cornusiin B were 3.07% in ethanolic extract of CA.

Neuroprotective mechanism of corydaline in glutamate-induced neurotoxicity in HT22 cells

  • Baskar Selvaraj;Dae Won Kim;Ki-Yeon Yoo;Keunwan Park;Thi Thu Thuy Tran;Jae Wook Lee;Heesu Lee
    • International Journal of Oral Biology
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    • v.49 no.1
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    • pp.10-17
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    • 2024
  • Glutamate-mediated oxidative stress causes neuronal cell death by increasing intracellular Ca2+ uptake, reactive oxidative species (ROS) generation, mitogen-activated protein kinase (MAPK) activation, and translocation of apoptosis-inducing factor (AIF) to the nucleus. In the current study, we demonstrated that corydaline exerts potent neuroprotective effects against glutamate-induced neurotoxicity. Treatment with 5 mmol/L glutamate increased cellular Ca2+ influx, ROS generation, MAPK activation, and AIF translocation. In contrast, corydaline treatment decreased cellular Ca2+ influx and ROS generation. Western blot analysis revealed that glutamate-mediated MAPK activation was attenuated by corydaline treatment. We further demonstrated that corydaline treatment inhibited the glutamate-mediated translocation of AIF to the nucleus. We propose that corydaline is a promising lead structure for the development of safe and effective neuroprotectants.

Analysis of Natural Pigment Indicator Components of Natural Dyed Cotton Fabrics Using HPLC-DAD-MS (HPLC-DAD-MS를 이용한 천연염색 면직물의 천연색소 지표성분에 대한 분석)

  • Lee, Won Kyoung;Kim, Jong Hoon;Kim, Moon Jung;Park, Yoon Cheol;Lee, Bum Hoon
    • Textile Coloration and Finishing
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    • v.32 no.4
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    • pp.255-264
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    • 2020
  • With the development of synthetic dyes, excellent dyeing properties and massive production became possible. On the other hand, natural dyeing technology has gradually disappeared. However, the international environmental and human safety requirements of the apparel and textile industry are increasing, and there is a growing interest in safer and more environmentally friendly products. Accordingly, the public's interest in natural dyeing is increasing. To meet these demands, it is first necessary to clarify the stages of production and distribution of natural dyes. It is necessary to evaluate whether the product is a natural dye product and study the suitability of the natural dye product through qualitative and quantitative assessment of the indicator components of the natural dye. Typical of three natural dyes did qualitative and quantitative analysis by HPLC-DAD and HPLC-MS. Products dyed using natural dye three products were analyzed, and the presence or absence of detection of each indicator components was confirmed to confirm whether the product was dyed using natural dyes.

Analysis of the Conditions and Products of Natural Dyeing Internet Shopping Malls (천연염색전문 인터넷 쇼핑몰 현황 및 상품 분석)

  • Lee, Mi-Suk;Chung, Kyung-Hee
    • Journal of the Korean Society of Clothing and Textiles
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    • v.34 no.7
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    • pp.1205-1219
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    • 2010
  • This study analyzes the conditions and products of internet shopping malls that sell natural dyeing products. In this study, 98 natural dyeing internet shopping malls were selected. The results of this study are as follows. The locations of the internet shopping malls were Gyeonggi-do, Seoul, Jeollanam-do, Gyeongsangbuk-do, Gwang-ju (city), Daegu (city), Busan (city), Gyeongsangnam-do, and Jeju-do. The most frequent dyes of the natural dyeing products were loess, followed by charcoal, indigo, and persimmon. Indigo was most frequently used in Seoul, with loess and charcoal most frequent in Gyeonggi-do. Persimmon, indigo, loess, and charcoal were mainly used in Jeollanam-do, with persimmon and loess in the Gyeongsangbuk-do, and persimmon in Jeju-do. The highest ordered product categories were accessories, followed by adult clothing, interior decoration products, and bedding. The most frequent products were bedclothes, followed by scarves, female shirts, blouses, pillows, female jackets, and vests. Regarding the price of products, 150,000-200,000 won was the highest for the Saenghwal Hanbok, with 10,000-30,000 won for underwear, 30,000-60,000 won for accessories, and 100,000-150,000 won for bedding. Concerning product information, 58.2% of internet shopping malls offer the product size and almost half of them did not show the properties or directions for handling the product. Based on the research results, the problems of the conditions and products of natural dyeing internet shopping mall were derived. The results show that the natural dyes of internet shopping malls lacked regional symbolism, the products were not specialized, and product information was not fully offered to consumers. To solve these problems, the strategies for marketing the promotion of the natural dyeing internet shopping mall were, ‘Using natural dyes from local resources’, ‘Market oriented and specialized item design’, and ‘Offer right product information’.