• Environmental Analysis Health and Toxicology
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• 제15권4호
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• pp.147-155
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• 2000

In order to evaluate anti -oxidant activities and protective effect against oxidatve damage, DPPH radical scavenging activity and lipid peroxidation inhibitory activity were measured among methanol extracts prepared from natural medicinal plants. Fourteen natural medicinal plants which were reported to have anti -oxidative or anti-inflammatory effects were selected based on our previous report. In addition to the total methanol extracts, n-hexane, dichloromethane, ethylacetate, n-butanol and water fractions were prepared from each total extract. DPPH radical scavenging assay was performed against 14 total extracts and all samples showed dose-dependent activities in various extent. Among those, 6 samples, methanol extracts of Euryale ferox, paeonia suffruticosa, Areca catechu var. dulcissima, Cinnamomun cassia, Alpinia katsumadai and Betula platyphlla var. japonica showed IC$\sub$50/ value lower than 6.0 $\mu\textrm{g}$/ml. The highest DPPH radical scavenging activity was found in ethylacetate fraction of paeonia suffruticosa with IC$\sub$50/ value of 1.1 $\mu\textrm{g}$/ml. Analysis of lipid peroxidation inhibitory activity on hydrogen peroxide-induced oxidative damage in Chinese hamster lung fibroblast (V79-4) cells revealed that the highest inhibitory effect was observed in methanol extract of Betula platyhpylla var. japonica. Lipid peroxidation inhibitory activity was observed as a dose-dependent manner in all samples used in this study. Among fraction samples, ethylacetate fraction of Alpinia katsumadai had the strongest inhibitory activity with IC$\sub$50/ value of 0.9 $\mu\textrm{g}$/ml.

• 마이크로전자및패키징학회지
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• 제7권4호
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• pp.7-15
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• 2000

질소분위기 중에서 솔더 접합부의 특성 및 solderability을 검토하기 위하여 대기분위기와 질소분위기에서의 젖음성을 평가하였다. 또한 솔더접합부의 브리지결함(bridge defect)을 대기와 질소분위기 중에서 비교 검토하였다. 이 결과, 질소분위기 중에서 Cu표면을 사포로 연마한 시편, Cu표면에 Sn-Pb 및 Sn으로 도금한 시편에서 젖음성이 향상되었다. 대기분위기에 비해 젖음시간은 약 0.2~0.45초 정도 시간이 감소하였고, 최대젖음력 ($F_{max}$)도 대기중에 비해 약 1.8~2.8 N 정도가 커졌다. 질소유량에 따른 젖음시간($t_2$)과 젖음력을 측정한 견과 질소유량이 10 1/min에서 30 1/min으로 증가함에 따라 대기중 분위기에 비해 젖음시간($t_2$)는 약 0.25초 정토 감소하였고, 젖음력은 2.3 N 정도 상승하였다. 따라서, 질소분위 기에서 무세정용 플럭스를 사용해도 젖음성이 떨어지는 것을 보완 할 수 있다. 질소분위기에서는 젖음성을 향상시키고 산화물(dross)를 억제시켜주어 대기 중 보다 브리지 (Bridge)발생률이 25~76%정도 떨어졌다. 브리지 발생률은 피치간격이 미세할수록 질소분위기가 대기 중 보다 감소하였다.

• 대한정형도수물리치료학회지
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• 제27권2호
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• pp.9-15
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• 2021

Background: This study investigated the effects of sustained natural apophyseal glides (SNAGS) technique on lower back pain and pain disability among university students with lower back pain. Methods: A total thirty 20~30 year old university students with lower back pain were divided into two groups. A lumbar flexibility exercise was applied to the control group (n=15). The SNAGS technique group (n=15) received L1~L4 segment mobilization using SNAGS technique. Intervention was implemented for 20 minutes, 3 times a week for 3 weeks. The SNAGS were performed using belt with flexion and extension, whereas lumbar flexibility exercise included lumbar flexion and extension. Lower back disability was measured using the Korean Oswestry disability index (KODI), and pain sensitivity was measured by pain pressure threshold (PPT). The groups were assessed for lower back pain disability and pain, before and after the intervention. Results: There were significant improvements in both the KODI and PPT of the SNAGS technique group (p<.05), while the lumbar flexibility exercise group showed no significant change (p>.05). In addition, the SNAGS technique group had a statistically significant difference in KODI and PPT compared to the lumbar flexibility exercise group (p<.05). Conclusion: SNAGS technique is more effective than lumbar spine joint flexibility exercise in the improvement of lower back pain disability and pain among university students with lower back pain.

• 한국토양비료학회지
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• 제36권3호
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• pp.119-126
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• 2003

질소 안정동위체 자연존재비(${\delta}^{15}N$)측정을 통해 노지 채소 재배토양에서 침출되어 지하로 이동하는 $NO_3-N$의 동태를 조사한 결과는 다음과 같다. 1) 3 m 관정수와 6 m 관정수의 $NO_3-N$ 농도 평균값은 각각 25.7 및 $2.8mg\;L^{-1}$이었으며, ${\delta}^{15}N$는 +3.6 및 +4.7‰이었다. 2) 암거배수의 $NO_3-N$ 농도와 ${\delta}^{15}N$의 평균은 각각 $35.5mg\;L^{-1}$와 +6.6‰로 토양 침투수는 즐인 선상지 전체로부터 모아지며 비교적 민감하게 화학비료, 가축분 퇴비 유래 질소농도의 변화를 반영하였다. 3) 단지 말단부의 용출수의 $NO_3-N$ 농도와 ${\delta}^{15}N$의 평균은 각각 $19.4mg\;L^{-1}$ 및 +7.9‰로서 채소 재배지대의 복류수 중에는 축산폐기물 유래가 침투되어 있는 것을 나타냈다. 4) 본 지구의 채소재배 토양의 ${\delta}^{15}N$은 2 N KCl 추출 가용태 질소로서 +6.1‰, 질산태 질소로서 +5.1‰이며, 주로 사용되는 화학비료의 전질소의 ${\delta}^{15}N$은 질산 억제비료가 -6.1‰, 그리고 완효성비료가 2.2‰이었다.

• Bulletin of the Korean Chemical Society
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• 제33권10호
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• pp.3248-3252
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• 2012

Vibrio extracellular metalloprotease (vEP), secreted from Vibrio vulnificus, shows various proteolytic function such as prothrombin activation and fibrinolytic activities. Premature form of vEP has an N-terminal (nPP) and a C-terminal (C-ter100) region. The nPP and C-ter100 regions are autocleaved for the matured metalloprotease activity. It has been proposed that two regions play a key role in regulating enzymatic activity of vEP. Especially, C-ter100 has a regulatory function on proteolytic activity of vEP. C-ter100 domain has been cloned into the E. coli expression vectors, pET32a and pGEX 4T-1 with TEV protease cleavage site and purified using gel-filtration chromatography followed by affinity chromatography. To understand how C-ter100 modulates proteolytic activity of vEP, structural studies were performed by heteronuclar multi-dimensional NMR spectroscopy. Backbone $^1H$, $^{15}N$ and $^{13}C$ resonances were assigned by data from standard triple resonance and HCCH-TOCSY experiments. The secondary structures of vEP C-ter100 were determined by TALOS+ and CSI software based on hydrogen/deuterium exchange. NMR data show that C-ter100 of vEP forms a ${\beta}$-barrel structure consisting of eight ${\beta}$-strands.

• Animal cells and systems
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• 제8권3호
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• pp.187-196
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• 2004

Two new species of siphonostomatoid copepods, Asterocheres tubiporae n. sp. and Entomopsyllus stocki n. sp., associated with the stoloniferan coral Tubipora musica (Linnaeus) are described from Madagascar. Asterocheres tubiporae is characterized by the possession of a large posteroventral process on the caudal ramus and the elongated free segment of leg 5. Entomopsyllus stocki is readily distinguished from its congeners by the possession in the female of 15-segmented antennule and three spines plus two setae on the third exopodal segment of leg 4.

• 대한수학회논문집
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• 제15권2호
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• pp.257-261
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• 2000

Northcott and Mckerrow proved that if R is a left noe-therian ring and E is an injective left R-module, then E[x-1] is an injective left R[x]-module. In this paper we generalize Northcott and McKerrow's result so that if R is a left noetherian ring and E is an in-jective left R-module, then E[x-S] is an injective left R[xS]-module, where S is a submonoid of N (N is the set of all natural numbers).

• 대한화학회지
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• 제31권3호
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• pp.258-270
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• 1987

여러 용매(methanol, ethanol, butanol, ethylether, methylacetate, acetone, THF 및 acetylacetone)중에서 아직 보고된 바 없는 공동을 달리한 crown ether들을 리간드로 한 토륨(IV) 고체 착물을 합성하였다. 이 고체 착물에 대한 배위 화학적인 정보를 얻기 위하여 원소분석(C.H.N) 및 ICPAS법에 의한 토륨분상, 열분석과 Karl-Fischer적정법에 의한 착물중의 결정수 분석에 의해서 고체 착물들의 조성을 결정하였다. 그리고 적외선, 자외선 분광분석, $^1H$-핵자기 공명 분석 및 X-선 회절분석법에 의하여 각 합성 착물들의 결합구조에 대한 정보들을 얻었고 각 합성 착물들에 대한 용매화 현상을 논의하였다. 그 결과 아세틸아세톤 용매하에서 합성된 착물들은 아세틸아세톤을 제외한 모든 실험 용매중에서 합성된 crown ether를 가지는 토륨(IV) 착물들은 용매화 반응이 일어나지 않았다. $Th^{4+}$금속이온에 대한 리간드 결합비는 고리의 크기에 주로 영향을 받으며 12-crown-4의 경우는 1:1([$Th^{4+}$]:[lig.]) 착물, 15-crown-5의 경우는 2:3 착물 및 18-crown-6와 dicyclohexano-18-crown-6의 경우는 1:1 착물이었다. 용매화가 일어나는 15-crown-5 와 18-crown-6 착물의 경우는 1:1:1([$Th^{4+}$]:[lig.]:$[CH_3COCH_2COCH_3]$)이었다. 용매화하지 않는 모든 착물들은 crown-ether의 n-${\sigma}^{\ast}$전자전이를, 그리고 용매화된 착물은 crown-ether n-${\sigma}^{\ast}$와 acac의 $n-{\pi}^{\ast}$전자전이를 동시에 나타냈다. 반양성자성 용매에서 모든 착물들은 1:1전해질로, 물에서는 1:4전해질로 각각 행동하는 9배위자 착물임을 알 수 있다.

• Archives of Pharmacal Research
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• 제24권4호
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• pp.270-275
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• 2001

Bioisostere approach has been shown to be useful to augment potency or to modify certain physiological properties of a lead compound. Based upon well documented bioisosterism, an isosteric replacement of benzene ring of 4-hydroxy-2-quinolone compound (L-695902) with a thiophene moiety was carried out to prepare the title compounds, 4-hydroxy-6-oxo-6,7-dihydro-thieno[2,3-b] pyrimidines 15. The resulting bioisosteric compounds 15 were evaluated for their antagonistic activity (birding assay) for NMDA receptor glycine site.

• Toxicological Research
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• 제13권1_2호
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• pp.23-27
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• 1997

This study was performed to examine the anti-cancer effect of Red Ginseng in the DENGalN-PH-induced hepatic tumor model system in rats. One hundred of male SPF Sprague-Dawley rats(6weeks old) were randomly divided into five groups. Rats in groups 1, 2, 3, and 4 were administered to diethylnitrosamine intraperitoneally 200 mg/kg body weight for the caner initiation. Rats in group 5 were given to saline as a control. On two weeks after cancer initiation, rats in groups 1 and 3 were fed on diet containing 0.01% of acethylaminofiuorene(AAF) which is strong cancer-promotor for 6 weeks, while rats in groups 2 and 4 were fed on water containing 0.05% of phenobarbital which is weak cancer.promotor for 6 weeks. Rats in groups 1 and 2 were treated with diet containing 3% of Red Ginseng for six weeks(from 9th week till 15th week after cancer initiation). Rats in all groups were necropsied time-sequencially at 8, 15, and 36 weeks. The hepatic lesions of rat treated with carcinogens expressed glutathione S-transferase placental form(GST-P) at 8 week. The GST-P positive foci of rats treated with AAF were larger than that of any other rats, while the GST-P positive foci of rats treated with AAF and red ginseng were significantly decreased. This anti-cancer effect of Red ginseng might be involved in the enhacement of natural killer cell activity. To know whether there is direct relationship between Red Ginseng and natural killer cell activity, the activity of natural killer cell was examined after treatment AAF, AAF+Red ginseng and Red ginseng only, respectively. Comparing with natural killer cell activity in AAF-treated group, natural killer cell activity was significantly activated in AAF+ Red ginseng-treated group. This indicated that Red ginseng might enhance natural killer activity after treatment carcinogen in rats. These results suggested that Red ginseng might have a cancer prevention ability by promoting natural killer cell activity during hepatocarclnogenesis.