• Korean Journal of Veterinary Research
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• v.27 no.2
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• pp.191-200
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• 1987

Attempts were to produce porcine leukocyte interferon(PorLeIF) and porcine immune interferon (PorIIF) in the culture of porcine leukocytes. The interferons produced were tested for antiviral activity against vesicular stomatitis virus on poreine-derived PK(15) cells, human-derived FL cells, and Korean native black goat-derived BGK cells. The results were summarized as follws: 1. In the isolation of porcine leukocytes, the mean isolation rate by the buffy coat separation method (28.7%) was higher than that by the hydroxyethyl starch-RBC sedimentation method (9.2%). 2. When NDV(BI)-induced PorLeIFs were assyed on PK(15) cells and FL cells, the mean titers were 129 IU/ml and 72 IU/ml respectively, being 55.8% of the activity in homologous species system expressed in heterologous system. 3. The activities of PHA P-induced PorIIFs were 197 IU/ml on PK (15) cells and no activity on human FL cells. The mean antiviral activity of PorIIF was 1.5 times that of PorLeIF in PK (15) cells. 4. The cytopathic effect of vesicular stomatitis virus was observed in BGK cells derived from Korean native black goat kidney permitting interferon assay on the cells. While the cross-species antiviral activity of reference human ${\alpha},\;{\beta}-interferon$ was observed on the cells, PorLeIF and PorIIF did not show any activity.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.2 no.2
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• pp.33-42
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• 1999

The objective of this study was to determine the optimal amount and the mixture ratio of seeds for revegetation species by investigating their growth characteristics. For the purpose of the experiments, 15 plants were chosen. In May of 1995 to September of 1998, the nursery seedbeds of the College of Agriculture and Life Sciences, Seoul National University were seeded with the mixtures of those species. Then, the nursery seedbeds were treated to investigate the effects of the amount of seeding, the amount of fertilizer, and the depth of soil on the growth characteristics. As the results of the experiments, it was found that the effects of all the treatments on growth of shoots and roots were significant in the early phase. For the expected number of seedlings more than 3,000 per square meter, many seedlings got withered to death while the survivors were suppressed to grow slow in the early stage due to the density problem.

• Preventive Nutrition and Food Science
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• v.11 no.2
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• pp.166-170
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• 2006

This study was carried out to investigate the fermentation characteristics and optimum conditions for alcohol fermentation of the Korean native mulberry. The yeast strains of S. kluyveri DJ97, La parisienne (Saccharomyces cerevisiae, Netherlands) and Enoferm (Saccharomyces cerevisiae, Denmark) produced higher alcohol concentrations than other strains, and further study was therefore performed with these three species. The optimum additional water content for maximizing alcohol concentration was 250% (v/w). The alcohol concentrations were rapidly increased in the first 4 days under the optimum conditions and reached 13.8% for S. kluyveri DJ97, 14.0% for La parisienne and 14.0% for Enoferm, respectively. Residual sugar concentration was decreased steadily from the beginning of fermentation until 5 days, after which it maintained a constant level. The pH was decreased steadily in the log phase during further maturation. However, the pH underwent a slight decrease after 4 days and then was stabilized during further maturation. Methanol concentrations for the three species used were analyzed after 60 days maturation and were lower than the levels regulated by the food standard. Fusel oils such as n-propanol, iso-butanol, and iso-amyl-alcohol were produced as by-products with the highest production from Enoferm and the lowest from S. kluyveri DJ97.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.6 no.5
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• pp.28-39
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• 2003

This study was carried out to elucidate effective restoration measures for natural forest with recycling native tree un site from November 2001 to October 2002 to obtain a basic information for revegetation measure, eight experimental treatment was done and the length of stump, root-ball size of stump, antisepsis treatment of trunk cut, Planting season and contents of organic matter in soil were effective on regrowth of root-stock. Thirteen tree species including Quercus acutissima among twenty tree species showed outstanding sprout and survival rate(over 90 percent), Planting in November and combinated planting with 5 trees and 9 shrubs of root-stock per 100$m^2$ plot showed a good growth. And 10 percent of organic matter plot showed a good crown coverage.

• Journal of the Korean Institute of Landscape Architecture
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• v.18 no.1
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• pp.41-51
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• 1990

This study was executed to investigate the vegetation structure and to propose the vegetation management proposals of the Sansung Nature Park at Cheonju City and the results were as fellows. 1. A damage of vegetation and soil surface around the Namgosa was taken place up to 100m by picnic 2. Pinus rigida forest covered 36.1% of the total area(152.6ha) and total afforested vegetation covered 43.5%, respectively. P. denstflora comm. covered 28.4% arid Carpinus laxiflora Comm. as a climax vegetation in temerate zone covered 0.3%, respectively. 3. 6, 7 and 8 of the degree of human disturbance of vegetation covered 45.3%, 26.6% and 21.4%, respectively and resulted in low quality of naturalness. 4. Physical and chemical properties of soil were pour at high elevation and were poor severely in and around the Namgosa due to human disturbance. 5. Plant community were under rapid succession and had unvalanced structure and heterogeneous composition of species. Rapid vegetational succession from Pinus densiflora and Pf. rigida to Carpinus laxifora, Quercus spp. and Robinina pseudo-acacia were taken place.6. The speeies of Raunkiaer's frequency class I as of high frequency class were Pinus dunsiflora, Carpinus alxiflora, Quercus mmongolica, Sorbus alnifolia, Prunrs sargintii, Rhododendron yedoense, Stephanandra incisa and Lespedeza maxinowiczii. 7. Vegetational management proposals were made for three vegetation zones ; Historic landscape restoration and preservation zone, Afforested vegetation zone, Native vegetation conservation zone and recommanded native species for landcape planting.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.17 no.1
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• pp.163-172
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• 2014

To investigate the possibility of developing the seeding measure for river bank slope revegetation, germination experiment and early growth observation were conducted using 3 native species growing naturally around river banks such as Pennisetum alopecuroides, Phragmites communis, and Miscanthus sinensis. The applied seeding methods were 3 such as scattering seeds, tillage after scattering seeds, and covering up seed with soil after scattering seeds. According to seeding methods, germination experiment and early growth observation were carried out on nursery bed soil in greenhouse. As results of this study, all the 3 native plant species' germination ratio and growth in length on nursery bed soil were highest on the seeding method of covering up seed with soil. Also it was verified by Duncan's multiple range test that the germination ratio and growth in length on the seeding method of covering up seed with soil is distinguished from those on other two seeding methods. According to this results, the best possible seeding measure to be developed should be mechanical seed spraying with soil.

• ALGAE
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• v.36 no.1
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• pp.13-24
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• 2021

The present-day genetic structure of macroalgal species reflects both geographical history and oceanic circulation patterns as well as anthropogenic introduction across native ranges. To precisely understand the genetic diversity and how the factors shape the current population structure of Gloiopeltis furcata sensu lato, we determined the mitochondrial 5' end of cytochrome c oxidase subunit I (COI-5P) sequences for 677 individuals sampled from 67 sites spanning almost the entire distribution range in Korea and Japan. Results from the phylogenetic analysis and haplotype distribution revealed eleven distinct lineages within G. furcata s.l. along the Korea-Japan coastal areas and displayed divergent phylogeographic patterns among lineages. Despite the closely related lineages distributed in same habitats as high rocky intertidal zone, they display different phylogeographic patterns among lineages. The populations from the south of Korea-Japan harbored the highest genetic diversity and unique endemism in comparison with other populations in the distribution range. This could be the evidence of southern refugia for G. furcata s.l. in the Northwest (NW) Pacific and the recent migration from native to introduced region. The reason is that an exceptional distribution pattern was found high genetic diversity in Hakodate of Japan where is the northern location in the NW Pacific. Our results imply the contemporary influence on the distribution due to current circulation pattern and anthropogenic effects. These phylogeographic findings provide the important insight into cryptic species diversity and the detailed distribution pattern of Gloiopeltis in the NW Pacific.

• Korean Journal of Environment and Ecology
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• v.24 no.3
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• pp.302-317
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• 2010

In this study, not only to present the management plan but also to verify the effectiveness for a area of improving the landscape and the area of creating the base of bio-inhabitation in Tancheon stream concrete embankment where were practised the vegetation-mat measures for greening via monitoring i.e. restoration progress. The results of monitoring, there were a total of 41 taxa, 18 families, 38 species, 3 varieties in 2006, moreover in the 2007, there were a total of 59 taxa, 19 families, 56 species, 3 varieties and in the period 2008, 64 taxa, 29 families, 59 species, 8 varieties. Therefore, these site has increased the plant spaces year by year. The distribution of vegetation characteristics shows that Miscanthus sacchariflorus and Pennisetum alopecuroides expands their influence in the area of applying the construction method. Those area appears a diversity of native species by the stream deposition at the flood. Thus, its condition is very soundly ecological health and eco-friend. At present, native species have been dominant, however, disturbed species and invasive species can be expected to increase dramatically in the future. Therefore, it is necessary to a long-range monitoring and management for maintaining an environmentally sound aquatic ecosystem. On this area refer to mix the river vegetation of primary succession and disturbed vegetation. For that reason, the method of constructing the vegetation-mat measures for greening in embankment does not need to remove the concrete and can install a coir-mat on the top. It leads to improve the landscape, moreover, it was analysed the such dramatic changes in the vegetation species richness by providing continuous the plant growth basis have a impact on in bio-diversity.

• Korean Journal of Plant Taxonomy
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• v.49 no.2
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• pp.118-126
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• 2019

The taxonomic rank of the tiny-leaved terrestrial orchid Cephalanthera subaphylla Miyabe & $Kud{\hat{o}}$ has been somewhat controversial, as it has been treated as a species or as an infraspecific taxon, under C. erecta (Thunb.) Blume [C. erecta var. subaphylla (Miyabe & $Kud{\hat{o}}$) Ohwi and C. erecta f. subaphylla (Miyabe & $Kud{\hat{o}}$) M. Hiro]. Allozyme markers, traditionally employed for delimiting species boundaries, are used here to gain information for determining the taxonomic status of C. subaphylla. To do this, we sampled three populations of five taxa (a total of 15 populations) of Cephalanthera native to the Korean Peninsula [C. erecta, C. falcata (Thunb.) Blume, C. longibracteata Blume, C. longifolia (L.) Fritsch, and C. subaphylla]. Among 20 putative loci resolved, three were monomorphic (Dia-2, Pgi-1, and Tpi-1) across the five species. Apart from C. longibracteata, there was no allozyme variation within the remaining four species. Of the 51 alleles harbored by these 17 polymorphic loci, each of the 27 alleles at 14 loci was unique to a single species. Accordingly, we found low average values of Nei's genetic identities (I) between ten species pairs (from I = 0.250 for C. erecta versus C. longifolia to I = 0.603 for C. falcata vs. C. longibracteata), with C. subaphylla being genetically clearly differentiated from the other species (from I = 0.349 for C. subaphylla vs. C. longifolia to 0.400 for C. subaphylla vs. C. falcata). These results clearly indicate that C. subaphylla is not genetically related to any of the other taxa of Cephalanthera that are native to the Korean Peninsula, including C. erecta. In a principal coordinate analysis (PCoA), C. subaphylla was positioned distant not only from C. falcata, C. longibracteata, and C. longifolia, but also from C. erecta. Finally, K = 5 was the best clustering scheme using a Bayesian approach, with five clusters precisely corresponding to the five taxa. Thus, our allozyme results strongly suggest that C. subaphylla merits the rank of species.

• Journal of Ecology and Environment
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• v.33 no.4
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• pp.363-376
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• 2010

Vegetative communities of created wetlands often display lower species richness, less cover, higher occurrence of non-native or invasive species, and fewer obligate wetlands species than those in natural wetlands, thus failing to meet basic success criteria for wetland mitigation. This study examined the effects of two design elements, disking-induced microtopography and hydrologic regime, on the first year vegetation development pattern of a mitigation wetland newly created in the Virginia piedmont. Elevation and species cover were measured along replicate multiscale circular transects in two adjacent wetland sites that are different in their hydrologic regime. Two microtopographic indices, tortuosity (T) and limiting elevation difference (LD), were calculated from the elevation measurements. Both indices were higher in disked plots than non-disked plots, showing the effect of disking on microtopography. Out of forty-one vegetation taxa observed in the wetland, 29 taxa were naturally colonized and 12 taxa were seeded. All plots except one non-disked plot were dominated by wetland vegetation. Species richness and diversity were higher in disked than in non-disked plots. Vegetation community development seemed also influenced significantly by hydrologic regime of the site. The effect of microtopography on species richness and diversity was more pronounced in a relatively dry site compared to a wet site. In addition, percent cover, species richness and diversity of vegetation were positively correlated with microtopographic indices such as T and LD. Two design elements, microtopography and hydrologic regime, should be considered and incorporated in wetland creation to enhance plant community development.