• Phonetics and Speech Sciences
• /
• v.12 no.4
• /
• pp.47-55
• /
• 2020

The current study was designed to investigate which type of phrase boundary tones high-intermediate Korean learners of English used in their spontaneous speech. These boundary tones were compared to those used in native speakers' spontaneous speech to examine whether the learners successfully acquired the use of boundary tones. To achieve this purpose, 10 Korean learners of English and four native speakers of English participated in the current study. The participants were asked to summarize the stories of short videos, and the tonal and the phrasing patterns of the obtained spontaneous speech were analyzed using Tone and Break Indices (ToBI) transcription conventions. The results indicated that both the native speakers and the Korean learners frequently marked their intonational phrase boundaries with high boundary tones. However, regarding the prosodic phrase positions within a sentence, Korean learners frequently used steep rising tones (i.e., H-H%) while native speakers used gradual rising tones (i.e., L-H%) for sentence-final intonational phrases. Overall, the findings suggested that high-intermediate Korean learners understood the forward-looking function of the high boundary tones and that they were able to make use of these tones to mark intonational phrases in their spontaneous speech.

• Proceedings of the Korean Society for Applied Microbiology Conference
• /
• 2001.06a
• /
• pp.149-153
• /
• 2001

To investigate the mode of bactericidal action for antimicrobial peptide, pediocin, synthetic and mutant pediocins were prepared by direct chemical synthesis. Native pediocin was purified from Pedio-coccus acidilactici M and its conformational structure and bactericidal functions were analyzed and compared to synthetic pediocin. Schematic mode of pediocin actions, how pediocin binds on the target cell membrane, penetrates and makes tunnel are proposed. For these purposes, primary and secondary structures of pediocin was analyzed and disulfide bond assignment was also done. The pediocin purified from P. acidilactici M had high effective bactericidal ability against gram positive bacteria, especially Listeria monocytogenes and was very stable at extreme pHs and even at high temperatures such as autoclaving temperature (121$^{\circ}C$). Pediocin was consisted of 44 amino acids with four cysteines. Novel synthetic peptides were achieved by solid phase peptide synthesis(SPPS) method. To explain the function of cysteine in C-terminal region, mutant pediocin, Ped[C24A＋C44A], was synthesized and their structural and biological functions were analyzed. Second mutant pediocin, Ped[KllE], was prepared to explain the function of lysine at 11 of N-terminal part of pediocin, especially loop of $\beta$-sheet, and to predict the initial binding site of pediocin. The native and synthetic pediocins was showed random coil conformation by spectropolarimetry in moderate conditions. This conformation was observed in extreme conditions such as high temperature and low and high pHs, also. Circular dichroism(CD) data also showed the existence of $\beta$-turn structure in N-terminal part both native and synthetic pediocins. A structural model for pediocin predicts that 18 amino acids in the N-terminal part of the peptide assume a three-strand $\beta$-sheet conformation. This random coil in C-terminal part of pediocin was converted to folding structure, helix structure, in nonpolar solvents such as alcohol and TFE. The disulfide bond between $^{9}$ Cys and $^{14}$ Cys was concrete and inevitable, however, evidences of disulfide bond between $^{24}$ Cys and $^{44}$ Cys was not. Data of Ped[C24A＋C44A], pediocin mutant showed that $^{44}$ Cys was required during killing the target cells but not inevitable, since Ped[C24A＋C44A] still have bactericidal activity but much less than native pediocin. Another pediocin mutant, Ped[KllE], had still bactericidal activity, was controversial to propose that positive charge like as $^{11}$ Lys in loop or hinge in bacteriocin bound or helped to binding to microorganism with electrostatic interaction between cell membrane especially teichoic acid and positive amino acid nonspecifically. The conformation of pediocin among native, synthetic and mutant pediocins did not show big difference. The conformations between oxidized and reduced pediocin were almost similar regardless of native or synthetic.

• Korean Journal of Veterinary Research
• /
• v.38 no.1
• /
• pp.17-28
• /
• 1998

Drug-metabolizing activities of Korean native cattle and swine were investigated from viewpoints of the cytochrome P-450's level, their dependent mixed function oxidase activities, the reactive oxygen species formation and cytosolic enzyme acitivities from each liver homogenates. Level of cytochrome P-450 in the liver microsome of Korean native cattle was $0.28{\pm}0.05nmole/mg$ and that in pigs $0.35{\pm}0.03nmole/mg$. Level of cytochrome $b_5$ of Korean native cattle was $0.24{\pm}0.06nmole/mg$, and that of pigs $0.2{\pm}0.05nmole/mg$, showing no difference between two species. NADPH P-450 reductase were higher in Korean native cattle ($58.3{\pm}5.3nmole/mg/min$) than in pigs ($29.9{\pm}3.8nmole/mg/min$)(p<0.01). The activities of cytochrome P-450 dependent monooxygenases such as ethoxyresorufin O-deethylase (cattle, $96.5{\pm}12.5nmole/mg/min$ ; pigs, $13.6{\pm}2.1nmole/mg/min$), N-benzphetamine N-demethylase (cattle, $5.23{\pm}0.82nmole/mg/min$ ; pigs, $0.76{\pm}0.3nmole/mg/min$) and aniline hydroxylase (cattle, $0.95{\pm}0.1nmole/mg/min$ ; pigs, $0.33{\pm}0.08nmole/mg/min$) were much higher in Korean native cattle than in swine(p<0.01). However, the activity of testosterone $7{\alpha}$-hydroxylase was higher in swine ($90.4{\pm}1.2nmole/mg/min$) than cattle (cattle, $32.8{\pm}1.2nmole/mg/min$). Interestingly, testosterone $16{\alpha}$-hydroxylase, a marker enzyme for P-450 IIA was not detected in both animal species. These results suggest that Korean native cattle and pigs have high contents of P-450 IA1 and P-450 IIIA. Total sulfhydryl compound (cattle, $10.3{\pm}1.1nmole/mg$ ; Pigs, $14.5{\pm}1.8nmole/mg$) and glutathione related enzymes except glutathione reductase (cattle, $38.1{\pm}7.9nmole/mg/min$; swine, $22{\pm}3.6nmole/mg/min$) showed higher levels in swine than in Korean native cattle. Superoxide dismutase (cattle, $7.64{\pm}0.84nmole/mg/min$ ; pigs, $4.47{\pm}0.94nmole/mg/min$) and catalase (cattle, $30.4{\pm}3.7nmole/mg/min$ ; pigs, $17.2{\pm}1.8nmole/mg/min$) were remarkably higher in Korean native cattle than in swine (p<0.05).

• Journal of applied mathematics & informatics
• /
• v.27 no.5_6
• /
• pp.1087-1095
• /
• 2009

In this paper, we study approximation method from scattered data to the derivatives of a function f by a radial basis function $\phi$. For a given function f, we define a nearly interpolating function and discuss its accuracy. In particular, we are interested in using smooth functions $\phi$ which are (conditionally) positive definite. We estimate accuracy of approximation for the Sobolev space while the classical radial basis function interpolation applies to the so-called native space. We observe that our approximant provides spectral convergence order, as the density of the given data is getting smaller.

• Journal of Sasang Constitutional Medicine
• /
• v.16 no.2
• /
• pp.17-31
• /
• 2004

1. Objectives This research is purposed to find Lee Je-Ma's thought on Inclination of Mind(心慾) and Nature & Emotion(性情), through summarizing sentences related to Inclination of Mind(心慾) and Nature & Emotion(性情) proposed in the chief writings on Sasang Constitutional Medicine. 2. Methods It was researched bibliologically with Lee Je-Ma's chief writings such as "Gyukchigo (Draft on Inquiring the Properties of Things, 格致藁)", "Dongyi Suse Bowon Sasang Chobongyun(東醫壽世保元四象草本卷)" "Dongyi Suse Bowon (Longevity and Life Preservation in Oriental Medicine, 東醫壽世保元)". 3. Results and Conclusions (1) Lee Je-Ma's Mind(心) is the dimension of the Great Absolute(Taegeuk,太極), and is related with Benevolence-Righteousness-Propriety-Wisdom(仁義禮智) (2) Human being is classified Taeyangin(太陽人), Taeumin(太陰人), Soyangin(少陽人), Soeumin(少陰人) by SungLi(性理), and The Mean(鄙人), TheWeek(薄人), The Avaricious(貪人), The Sycophantic(懦人) by JungYok(情慾) in Sasang Constitutional Medicine. (3) Nature & Emotion(性情) of Sorrow-Anger-Joy-Pleasure(喜怒哀樂) is divided into two function. One is native function related with SungLi(性理), the other is acquired function related with JungYok(情慾). Also all two native and acquired function is related with Benevolence-Righteousness-Propriety-Wisdom(仁義禮智). (4) The Mean(鄙人), The Week(薄人), The Avaricious(貪人), The Sycophantic(懦人) may be defined as the state of Inclination of Mind(心慾) of Taeyangin(太陽人), Taeumin(太陰人), Soyangin(少陽人), Soeumin(少陰人) by function of acquired Emotion(情).

• Korean Journal of Animal Reproduction
• /
• v.17 no.4
• /
• pp.325-329
• /
• 1994

Seasonal and diurnal variation in serum testosterone was measured by radioimmunossary in 3 mature Korean native male goats. Blood was collected at 3-h intervals for 24h in each season. Mean levels of 4 seasons ranged from 0.82 to 2.06ng/ml and no difference was among seasons, although level in fall and winter was slightly higher. Diurnal level tended to increase at daytime in all season and when diurnal variation in level was compared to daily mean of each season, level of daytime greatly(P<0.05) increased in summer. However, variation between light and dark period in other seasons did not show significant difference. Daytime increase of testosterone was found 3 to 4 after dawn and level increased 1 to 2h ahead in summer and fall than that in others. These results in serum testosterone suggest that there was no great seasonal variation in endocrine function of goat testis.

• BMB Reports
• /
• v.31 no.5
• /
• pp.492-497
• /
• 1998

Cu,Zn-superoxide dismutase (SOD) was purified from horseradish by using Mono Q and Superose 12 FPLC column chromatography. The native molecular mass of the purified enzyme was approximately 33 kDa, as determined by gel filtration. The subunit molecular weight, as estimated by SDS-PAGE, was 16 kDa. These results indicated that the native enzyme is a homodimer. We investigated the free radical-generating function of horseradish Cu,Zn-SOD by using a chromogen, 2,2'-azinobis-(3-ethylbenzthiazoline-6-sulfonate) (ABTS) which reacts with ${\cdot}OH$ radicals to form $ABTS^{+{\cdot}}$ The formation of $ABTS^{+{\cdot}}$ was required for both active Cu, Zn-SOD and $H_2O_2$. The optimal pH for the free radical-generating activity of this enzyme was 6.0-8.0, and it retained about $40^{\circ}C$ of its maximum activity when exposed at $40^{\circ}C$ for 15 min. A neutral scavenger, ethanol, inhibited the $ABTS^{+{\cdot}}$ formation by horseradish Cu, Zn-SOD more effectively than that by the mammalian enzyme. These results suggest that the active channel of horseradish enzyme is slightly larger than that of the mammalian enzyme.

• Korean Journal of Animal Reproduction
• /
• v.18 no.4
• /
• pp.245-250
• /
• 1995

The appearance and function of corpora lutea(CL) with a central cavity in the ovaries of Korean Native Cattle (KNC) were investigated endocrinologically and histochemically. The CL were enucleated from KNC ovaries within 2~3 hrs local slaughter house and classified with central cavity CL or not. Enzymatically dispersed luteal cell (1$\times$106 live cell/ml of Dulbecco's Modified Eagle Media) with or without cavity of CL cultured at 37$^{\circ}C$ in a humidified incubation (5% CO2 : 95% air) for 72hr. A central cavity in the CL of KNC was found in 58.8% of CL-1, 34.9% of CL 2, 39.1% of CL-3, and 11.1% of CL-4, respectively. There were no significant difference between protein content of CL with and without a central cavity. Mean progesterone secretion after 36h of in vitro luteal cell culture were significantly (p<0.05) higher in CL with central cavity than without cavity. However, the luteal cavitic wall was composed of the connective tissue band of the reticular and collagen fibers and then these connective tissue band extended into the CL with cavity. These results suggest that the central cavity of CL may be caused infertility in KNC.

• Journal of National Security and Military Science
• /
• s.2
• /
• pp.237-260
• /
• 2004

As the research work about XML based on the development of Internet and according to the information exchange standard is being carried out, the need of discovering new methods to store XML documents and manage them efficiently according to the frequency of large-capacity XML documents increases. Consequently, as a kind of back-end database system, XML storage systems such as RDBMS, OODBMS and Native XML DBMS etc. are coming forth in order to save XML documents. It is an urgent task to make comparisons among usage expense, function comparison storage, inquiry, and manage dimension for each DBMS. This paper makes an analysis and comparison of DTD-independent XML document access methods in RDBMS, OODBMS and Native XML DBMS for XML storage and management. After analyzing the advantages and disadvantages of each access method and comparing the function of typical commerce DBMS such as Oracle 8i, eXcelon and Tamino for finding the possibility of military applications, an another appropriate method to save XML documents is proposed as to find an implementation approach to save structural XML documents.

• Food Science of Animal Resources
• /
• v.33 no.3
• /
• pp.331-334
• /
• 2013

Osteopontin (OPN) is a secreted phosphorylated glycoprotein. It has an important role in multiple biological processes including cell survival, bone remodeling, inhibition of ectopic calcification, as well as, is thought to have potential immune modulation activities. In this work, we isolated and characterized a full-length open reading frame (ORF) of Korean native cow's OPN from Korean native cow's (Hanwoo) kidney, and successfully cloned firstly on Hanwoo's OPN. The sequencing results indicated that the isolated cDNA was 1190 bp in length containing a complete ORF of 837 bp. It encoded a precursor protein Hanwoo's OPN consisting of 278 amino acids with a signal peptide of 16 amino acids. Amino acid homology was found to be 99.3% as compared to the corresponding sequences of Holstein bone marrow OPN. Hanwoo's kidney OPN and Holstein bone marrow OPN are different only in two amino acid residues 42 and 56, amino acid residue 42 is Thr (T) ${\leftrightarrow}$ Ile (I), and amino acid residue 56 is Ala (A) ${\leftrightarrow}$ Thr (T) respectively. These results from the present work would be helpful to elucidate the biological function of Hanwoo's OPN and provided a foundation for further insight into role of Hanwoo's OPN.