• Title/Summary/Keyword: National Resource

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Chemical constituents of Dicentra spectabilis and their anti-inflammation effect

  • Kim, A Hyeon;Jang, Ji Hun;Woo, Kyeong Wan;Park, Jong Eel;Lee, Ki Ho;Jung, Ho Kyung;An, Byeongkwan;Jung, Won Seok;Ham, Seong Ho;Cho, Hyun Woo
    • Journal of Applied Biological Chemistry
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    • 제61권1호
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    • pp.39-46
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    • 2018
  • Column chromatographic separation of the MeOH extract from the roots of Dicentra spectabilis yielded fourteen compounds, menisdaurin (1), menisdaurilide (2), trans-N-p-coumaroyltyramine (3), trans-N-p-feruloyltyramine (4), 4-O-feruloylquinicacid (5), chlorogenic acid (6), 3-O-feruloylquinicacid (7), ferulic acid (8), protopine (9), Kaempferol 3,7-di-O-${\beta}-{\text\tiny{D}}$-glucopyranoside (10), kaempferol 3-O-${\beta}-{\text\tiny{D}}$-glucopyranosyl-7-O-${\alpha}-{\text\tiny{L}}$-rhamnopyranoside (11), ${\alpha}-rhamnoisorobin$ (12), astragalin (13), and nicotiflorin (14). Their structures were determined on the basis of NMR spectroscopic data. Among them, compound 1, 3-8, and 10-14 isolated from this plant were reported for the first time. The isolated compounds (1-14) were tested for nitric oxide (NO) inhibitory activity on lipopolysaccharide-stimulated RAW 264.7 cells. Compound 3, 4 and 12 significantly inhibited NO production. Moreover, Compound 3 suppressed pro-inflammatory cytokines ($TNF-{\alpha}$, $IL-1{\beta}$ and IL-6) in a dose- dependent manner. These data suggest that compound 3 possess anti-inflammatory activity and might be useful natural materials for development of anti-inflammatory agent.

Two New Records of Ascomycetes from Crop Field Soils in Korea

  • Adhikari, Mahesh;Kim, Hyun Seung;Gurung, Sun Kumar;Bazie, Setu;Lee, Hyun Gu;Lee, Hyang Burm;Lee, Youn Su
    • 한국균학회지
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    • 제45권3호
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    • pp.167-174
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    • 2017
  • In the ongoing survey of fungal diversity in Korea, two Ascomycetes species, namely Rhinocladiella similis and Toxicocladosporium irritans, were isolated in 2016. These species were identified based on the internal transcribed spacer region and morphological characteristics. This is the first report of these species in Korea, and the morphological characteristics and images of the fungi are presented.

First Report of Metacordyceps chlamydosporia (Cordyceps chlamydosporia) Isolated from Soil in Korea

  • Kim, Hyun Seung;Adhikari, Mahesh;Yadav, Dil Raj;Kim, Sang Woo;Um, Yong Hyun;Lee, Hyang Burm;Lee, Youn Su
    • 한국균학회지
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    • 제44권1호
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    • pp.48-50
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    • 2016
  • A previously unrecorded species, Metacordyceps chlamydosporia KNU14-22, was isolated from soil in Korea. Identification of the fungal species was based on morphological and molecular characteristics. This species has not been previously reported in Korea and herein we present data with detailed descriptions and figures.

A New Record of Pseudeurotium bakeri from Crop Field Soil in Korea

  • Adhikari, Mahesh;Kim, Sangwoo;Yadav, Dil Raj;Um, Yong Hyun;Kim, Hyun Seung;Lee, Hyang Burm;Lee, Youn Su
    • 한국균학회지
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    • 제44권3호
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    • pp.145-149
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    • 2016
  • Pseudeurotium bakeri KNU14-21 isolated from crop field soil is a previously unreported fungal species in Korea. This fungal species was identified through morphological and molecular characteristics. Based on the shape and size of conidia and conidiophores as well as the internal transcribed spacer region of rDNA, the isolate was confirmed to be Pseudeurotium bakeri. This species has not been previously reported from Korea, and this is the first report of its incidence in Korea.

Phialocephala lagerbergii: A New Record from Crop Field Soil in Korea

  • Adhikari, Mahesh;Kim, Sangwoo;Yadav, Dil Raj;Um, Yong Hyun;Kim, Hyung Seung;Lee, Hyang Burm;Lee, Youn Su
    • 한국균학회지
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    • 제44권3호
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    • pp.132-137
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    • 2016
  • A unrecorded hyphomycete species of Phialocephala was isolated for the first time during the investigation of fungal community in the soil samples collected from different regions of Korea. The fungal isolate was identified as Phialocephala lagerbergii, based on the morphological characteristics and phylogenetic analysis of the ribosomal DNA sequence. In addition, cultural and micro-morphological features were described in detail.

자원 순환형 학교 폐기물 관리 방안 연구 (A Study of the Resource-Recycling Waste Management at Schools)

  • 남영숙;지승현;우정애;조이상;안영희
    • 한국환경교육학회지:환경교육
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    • 제19권2호
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    • pp.69-80
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    • 2006
  • A 'Resource recycling society' is the common goal to reduce environmental impact from human activities and to conserve natural resources. The Resource-Recycling Waste Management is necessary to explicitly show objectives, and to evaluate the achievement level gained by school administration. In this paper, focusing on resource-recycling school waste management, three indicators are designed to evaluate the present conditions of school waste management, which include evaluation of school policy, waste management and education for waste management by school administration. Each indicator is made up of three or four questions that are supposed to be answered by school managers, teachers and students. Questionnaire surveys, which is based on the resource-recycling school waste management indicators, were carried out in elementary school, middle school and high school in the National Capital region. The resulting of indicators well describe the situation of school waste management among these schools. Influential strategies towards resource-recycling school waste management have been found in analysis.

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Effect of Activation Time on the Nuclear Remodeling and In Vitro Development of Nuclear Transfer Embryos Derived from Bovine Somatic Cells

  • Choi, J. Y.;J. W. Cho;D. J. Kwon;Park, C. K.;B. K. Yang;Kim, C. I.;H. T. Cheong
    • 한국동물번식학회:학술대회논문집
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    • 한국동물번식학회 2002년도 춘계학술발표대회 발표논문초록집
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    • pp.6-6
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    • 2002
  • This study was conducted to investigate the effect of recipient activation time on the nuclear remodeling, chromatin structure, pronuclear formation and in vitro development of bovine nuclear transfer embryos derived from adult ear skin cells. Somatic cells were transferred to enucleated oocytes after quiescent treatments by serum starvation or culture to confluency. Nuclear transfer embryos were activated with a combination of Ca/sup 2+/-ionophore and cycloheximide at 1, 1.5, 2, 2.5, 3, and 5 h after electrofusion. (omitted)

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여자시스템 정지시 문제점에 대한 분석 (The Analysis on Stopping Trouble for Excitation System)

  • 옥연호;이은웅;김기원;조남빈;변일환;최홍열
    • 대한전기학회:학술대회논문집
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    • 대한전기학회 2007년도 제38회 하계학술대회
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    • pp.1073-1074
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    • 2007
  • The Analysis on stopping trouble for Excitation System The excitation system must be stopped stably on generator's stopping. Therefore through studying on Excitation Stopping scheme, and problems which might happen when stopping, we would like to enhance the stability of generator and control system.

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방울비짜루 지표성분의 함량분석과 항산화·항염증 활성 (Quantitative Analysis of Chemical Composition and In Vitro Anti-oxidant and Anti-inflammatory Properties of Asparagus oligoclonos)

  • 이현주;정다은;강주은;심미옥;성태경;우경완;안병관;정호경;조현우
    • 생약학회지
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    • 제49권2호
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    • pp.138-144
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    • 2018
  • Asparagus oligoclonos is one of the endemic halophytes used folk medicine in Korea. We isolated the main compound rutin from methanol extracts of A. oligoclonos based on nuclear magnetic resonance and TOF ESI-MS data. We have investigated the quantitative analysis method of main compound using HPLC and the results exhibit that rutin content of A.oligoclonos were 1.816%. To explore anti-oxidant from A. oligoclonos ethanol extracts (AOE), we investigated the antioxidant effects of AOE on $H_2O_2$-induced oxidative stress in RAW 264.7 cell. AOE were reduced $H_2O_2$-induced oxidative stress via enhancement of cell viability, and AOE significantly decreased ROS production depending on concentration. Next, to screen for anti-inflammatory activity of AOE, we investigated the inhibitory effects of AOE in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. AOE had no effect on cell viability at a concentration of $500{\mu}g/mL$. Nitric oxide (NO) production inhibited in a dose-dependent manner. These results suggest that AOE may be a useful antioxidant and anti-inflammatory agent.