Proceedings of the KSAR Conference (한국동물번식학회:학술대회논문집)

The Korean Society of Animal Reproduction (한국동물번식학회)
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Effect of Activation Time on the Nuclear Remodeling and In Vitro Development of Nuclear Transfer Embryos Derived from Bovine Somatic Cells

  • Choi, J. Y. (Institute of Human Infertility and Genetics, Saewha Women′s Clinic) ;
  • J. W. Cho (College of Animal Resource Science, Kangwon National Universit) ;
  • D. J. Kwon (College of Animal Resource Science, Kangwon National Universit) ;
  • Park, C. K. (College of Animal Resource Science, Kangwon National Universit) ;
  • B. K. Yang (College of Animal Resource Science, Kangwon National Universit) ;
  • Kim, C. I. (College of Animal Resource Science, Kangwon National Universit) ;
  • H. T. Cheong (College of Animal Resource Science, Kangwon National University)
  • Published : 2002.06.01
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Abstract

This study was conducted to investigate the effect of recipient activation time on the nuclear remodeling, chromatin structure, pronuclear formation and in vitro development of bovine nuclear transfer embryos derived from adult ear skin cells. Somatic cells were transferred to enucleated oocytes after quiescent treatments by serum starvation or culture to confluency. Nuclear transfer embryos were activated with a combination of Ca/sup 2+/-ionophore and cycloheximide at 1, 1.5, 2, 2.5, 3, and 5 h after electrofusion. (omitted)

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