• 임상간호연구
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• 제26권3호
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• pp.337-343
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• 2020

Purpose: This study is a descriptive study to identify the degree of workplace bullying experience of newly graduated nurses and the difference in coping and adaptation processing, organizational socialization in relation to workplace bullying experience. Methods: The Study participants were newly graduated nurses with 3 months to 1-year clinical experience (N=186) and conveniently sampled from a small to medium-sized hospital located in G Metropolitan City. Data were collected in July 2018, using structured questionnaires. Results: Half of the participants (50.0%) reported workplace bullying experience. Most bulling experiences were person or work related. Newly graduated nurses who experienced bullying in the workplace demonstrated significantly lower coping and adaptation process (t=3.34, p=.001) and organizational socialization (t=7.46, p=<.001) than nurses who did not experience bullying in the workplace. Conclusion: Orientation programs for newly graduated nurses need to include contents that can improve the coping and adaptation process. Nursing managers at small and medium-sized general hospitals should actively support the mentor-mentee program to promote the organizational socialization of newly graduated nurses.

• 한국응용과학기술학회지
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• 제38권6호
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• pp.1596-1605
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• 2021

본 연구는 간호대학생의 의사소통능력과 긍정적 사고 및 대인관계와의 관련성을 파악하고 의사소통능력에 영향을 미치는 요인을 확인하기 위한 서술적 조사연구이다. 연구대상자는 G시와 P시에 소재하는 2개 대학 간호학과 학생 171명이었고 구조화된 설문지를 이용하여 조사하였다. 수집된 자료는 빈도와 백분율, t-test, ANOVA, Pearson's 상관관계 분석 및 다중회귀분석을 이용하여 분석하였다. 간호대학생의 의사소통능력에 미치는 영향요인을 확인하기 위해 다중회귀분석 결과, 대인관계(β=.404, p<.001)가 큰 영향을 보이는 변수로 나타났고, 긍정적 사고(β=.274, p=.014)가 그 다음 순서로 나타났다. 회귀모형의 적합도에 대한 F 통계량은 20.940(p<.001)로 유의하였고, 설명력은 47.9%인 것으로 나타났다. 그러므로 간호대학생의 의사소통능력 향상을 위해서는 대인관계와 긍정적 사고를 높일 수 있는 다양한 방안을 모색해야 할 것이다. 본 연구는 간호대학생의 의사소통능력을 향상시키기 위한 프로그램을 개발하는데 기초자료로 유용하게 활용될 것이다.

• 임상간호연구
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• 제28권2호
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• pp.177-184
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• 2022

Purpose: The purpose of this study was to identify the relationships between variables and factors affecting the intention on retention of nurses in small and medium-sized hospitals. Methods: The participants of this study were 203 nurses working in six small and medium-sized hospitals, and data was collected from August 15 to August 30, 2019 using a structured questionnaire. The collected data were analyzed by t-test, ANOVA, Pearson's correlation coefficients, and multiple regression using IBM SPSS/WIN 24.0. Results: As a result of the study, intention on retention was positively correlated with resilience (r=.40, p<.05) and followership (r=.38, p<.05). Affecting factors of the intention on retention were marital state (married) (β=.21, p=.001), resilience (β=.22, p=.016), and active engagement as a subcartegory of followership (β=.19, p=.034), and the variables explained 20.8% of the nurses' intention on retention. Conclusion: In order to increase the intention on retention of small and medium-sized hospitals nurses, it is necessary to provide an environment in which small and medium-sized hospital nurses are able to have active engagement and independent thinking and to develop a program to promote their resilience.

• Plant Biotechnology Reports
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• 제3권4호
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• pp.277-283
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• 2009

One of the limitations to conducting maize Agrobacterium-mediated transformation using explants of immature zygotic embryos routinely is the availability of the explants. To produce immature embryos routinely and continuously requires a well-equipped greenhouse and laborious artificial pollination. To overcome this limitation, an Agrobacterium-mediated transformation system using explants of type II embryogenic calli was developed. Once the type II embryogenic calli are produced, they can be subcultured and/or proliferated conveniently. The objectives of this study were to demonstrate a stable Agrobacterium-mediated transformation of maize using explants of type II embryonic calli and to evaluate the efficiency of the protocol in order to develop herbicide-resistant maize. The type II embryogenic calli were inoculated with Agrobacterium tumefaciens strain C58C1 carrying binary vector pTF102, and then were subsequently cultured on the following media: co-cultivation medium for 1 day, delay medium for 7 days, selection medium for $4{\times}14$ days, regeneration medium, and finally on germination medium. The T-DNA of the vector carried two cassettes (Ubi promoter-EPSPs ORF-nos and 35S promoter-bar ORF-nos). The EPSPs conferred resistance to glyphosate and bar conferred resistance to phosphinothricin. The confirmation of stable transformation and the efficiency of transformation was based on the resistance to phosphinothricin indicated by the growth of putative transgenic calli on selection medium amended with $4mg\;1^{-1}$ phosphinothricin, northern blot analysis of bar gene, and leaf painting assay for detection of bar gene-based herbicide resistance. Northern blot analysis and leaf painting assay confirmed the expression of bar transgenes in the $R_1$ generation. The average transformation efficiency was 0.60%. Based on northern blot analysis and leaf painting assay, line 31 was selected as an elite line of maize resistant to herbicide.

• 대한통합의학회지
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• 제12권1호
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• pp.161-169
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• 2024

Purpose : This study investigated the correlation between social support and older people's quality of life. Methods : We conducted a questionnaire survey with 350 older participants and analyzed 320 completed surveys. The collected data were analyzed using SPSS 25.0 for Windows. The general characteristics of the participants and quality of life subdomains were analyzed using descriptive statistics and frequency analyses. Furthermore, the differences between the subdomains of social support and quality of life were analyzed using independent t-tests and a one-way ANOVA. Post-hoc tests were performed using the Scheffé test. A significance level of α=.05 was used to verify statistical significance. Results : Upon examining the sub-domain level of social support and considering general characteristics, it was found that older age correlates with increased dependency among patients with associated diseases (p<.05). Similarly, an investigation into participants' quality of life at the subdomain level, considering general characteristics, revealed higher correspondence to lower quality of life (p<.05). Differences emerged between social support and quality of life among older individuals, with a negative correlation observed between the two (p<.05). Conclusion : This study suggests the importance of actively supporting older people with chronic diseases who experience emotional and psychological instability. It advocates for promoting the use of various services, including visiting medical services, convalescent care, and assistance, to meet their needs effectively.

• Journal of Plant Biotechnology
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• 제34권4호
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• pp.375-380
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• 2007

국내 옥수수 순계주에서 Agrobacterium 공동배양으로 CP4 5-Enol-pyruvylshikimate-3-phosphate synthase (CP4 EPSPS) 유전자가 도입된 제초제저항성식물체를 개발하였다. 5개의 순계주 (HW1, KL103, HW3, HW4, HW7)의 미숙배를 Ubiquitin promoter-CP4 EPSPS 유전자와 CaMV35S promoter-nptII 유전자가 발현되도록 제조된 pCAMBIA2300 벡터를 C58C1 Agrobacterium에 형질전환하여 공동 배양하였다. 항생제로 paromomycin이 첨가된 배지에서 선발된 옥수수 형질전환체를 PCR, RT-PCR 및 Northern 분석을 통하여 유전자의 도입과 발현을 확인하였다. 또한 형질전환 식물체의 glyphosate 처리에 따른 shikimate 축적반응을 확인하였다. Paromomycin 저항성 캘러스 형성빈도는 옥수수 각 순계주 HW1, KL103, HW3, HW4, HW7에서 각각 0.37%, 0.03%, 2.20%, 2.37%, 0.81%로 나타났으며, PCR분석을 통하여 최종적으로 2개의 옥수수 순계주 (HW3, HW4)의 paromomycin 저항성 캘러스로부터 분화된 식물체에서 확인하였다. 이러한 형질전환체중에서 RT-PCR 및 Nothern blot 분석을 통하여 CP4 EPSPS 유전자가 발현되는 2개의 계통 (M266, M104) 을 선발하였고, shikimate 축적반응을 통하여 glyphosate에 대한 저항성을 갖는 계통 (M266)을 최종적으로 선발하였다. 이러한 결과는 국내 옥수수 순계주에서 제초제저항성을 갖는 옥수수 형질 전환체를 개발할 수 있음을 시사한다.

• 대한통합의학회지
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• 제10권4호
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• pp.153-163
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• 2022

Purpose : The purpose of this study was to investigate the differences between uncertainty of chronic diseases and quality of life with regard to elderly people. Methods : The participants of this study were 350 elderly people, aged over 65 years. The collected data were analyzed using the SPSS Window program and the general characteristics of the participants and sub-domains of quality of life were analyzed by several frequency analyses and descriptive statistics such as mean, standard deviation, skewness, and kurtosis. Further, the differences between the sub-domains of uncertainty and sub-domains of quality of life were analyzed through independent t-test and one-way ANOVA. In order to reach conclusive results, post-test was analyzed by the Scheffe test. In addition, Pearson's correlation analysis was performed to determine the correlation between the target categories. A significance level of 𝛼=.05 was used to verify statistical significance. Results : As a result of examining "uncertainty" with respect to general characteristics, such as older age, low educational background, and chronic diseases, it was observed that the more intense these factors became, the more the level of uncertainty increased. In addition, it was also noted that except "accompanying diseases" in social domain, the participants enjoyed a high level of quality of life. The correlation was noted between domains of complexity and sociality (p<.01), domains of inconsistency and sociality (p<.01), domains of complexity and living environment (p<.01), domains of inconsistency and living environment (p<.01), and total score of uncertainty and total quality of life (p<.01). Conclusion : In this study, differences were found between "uncertainty" and "quality of life" of elderly people; the correlation between the sub-domains based on general traits was found to be negative (-). This suggests that objective evidence can be presented for the prevention of diseases by using mental health programs for the elderly in future.

• 한국학교보건학회지
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• 제32권2호
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• pp.67-76
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• 2019

Purpose: The purpose of this study was to explore self-directed learning ability and its affecting factors among undergraduate students. Methods: A descriptive cross-sectional study design was used. Using a convenience sampling method, data were collected from 196 undergraduate students enrolled in one national university. Negative psychological tendency (i.e. Type D personality), academic engagement, and self-directed learning ability were assessed using a structured questionnaire. Data were analyzed by descriptive statistics, t-test, and analysis of covariance, Pearson's correlation coefficients, and stepwise multiple linear regression, using SPSS/WIN 23.0 program. Results: The mean age of the students was $21.61{\pm}2.40years$ and 56.6% were male students. Approximately, one third (n=67, 34.2%) of the students had Type D personality. The average scores of academic engagement and self-directed learning ability were $3.01{\pm}1.14$ and $3.46{\pm}0.50$, respectively. After controlling for sociodemographic variables, the Type D personality and academic engagement were significant predictors of self-directed learning ability (${\beta}=.64$, p<.001; ${\beta}=-.13$, p=.021, respectively). This model explained 53.6% of the variance in self-directed learning ability. Conclusion: The study identified that Type D personality and academic engagement affect self-directed learning ability of undergraduate students, one in a negative way, the other in a positive way. Educators and educational policy makers need to make efforts to include interventions and strategies that increase academic engagement and change negative psychological dispositions such as D-type personality in the undergraduate education curriculum.

• 디지털융복합연구
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• 제18권11호
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• pp.547-558
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• 2020

본 연구는 신규간호사의 직무스트레스의 예측요인을 규명하여 중재전략을 개발하는 기초자료를 제시하고자 시도된 융합연구이다. 연구대상자는 자기 보고식 설문지를 활용하여 신규간호사 170명을 대상으로 자료 수집을 하였다. 자료 분석은 빈도, 백분율, independent t-test, ANOVA, Scheffe' test, Pearson's correlation, stepwise regression을 시행하였다. 연구 결과 신규간호사의 직무스트레스의 가장 큰 예측요인으로는 이직의도(β=.481)가 직무스트레스에 대하여 가장 중요한 변수로 나타났으며, 감정노동, 대인관계, 이직의도 3개의 설명변수의 직무스트레스(R²= .572, F=55.10, p<.001)에 대한 설명력은 57.2%였다. 이상의 결과로 직무스트레스 관리를 위해 감정노동과 대인관계능력 향상 및 조기에 이직의도를 발견하고 신규간호사의 직무스트레스를 관리하기 위한 중재프로그램 개발이 필요하며, 향후 신규간호사의 직무스트레스 예측요인 확인을 위한 반복연구가 필요하다.

• 한국산학기술학회논문지
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• 제15권7호
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• pp.4395-4403
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• 2014

본 연구의 목적은 무릎관절 전치환술을 시행한 여성 환자에게 슬링 운동을 적용하여 무릎관절의 근력과 관절가동범위에 미치는 영향을 비교하기 위해 실시하였다. 참여자는 2그룹 나누어 슬링 운동군 15명, 대조군 15명으로 무작위로 배정하였다. 대상자들은 근력 검사를 위해 Biodex system를, 관절가동범위 검사를 위해 각도기를 사용하여 평가하였다. 데이터의 분석은 통계적 유의 수준을 결정하기 위해서 대응표본 t-검정과 독립 t-검정을 이용하였다. 본 연구의 결과 슬링 운동군은 중재 전후 굽힘 각, 넙다리네갈래근, 뒤넙다리근 근력이 통계학적으로 유의한 증가를 보였고, 대조군은 중재 전후 굽힘 각, 폄 각, 넙다리네갈래근, 뒤넙다리근 근력이 통계학적으로 유의한 증가를 보였다. 무릎관절 근력과 굴곡 관절 가동범위는 슬링 운동군이 대조군에 비해 통계학적으로 유의하게 증가하였다. 슬링 운동법이 무릎관절 전치환술 환자의 근력과 관절 가동범위에서 긍정적인 영향이 있음을 알 수 있었다.