• The Plant Pathology Journal
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• v.27 no.4
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• pp.385-389
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• 2011

A new reverse transcription loop-mediated isothermal amplification (RT-LAMP) method for the Potato leafroll virus (PLRV) was developed and compared with conventional reverse transcription polymerase chain reaction (RT-PCR) to address its advantages over RTPCR. RT-LAMP primers were designed from the open reading frame 3 (ORF3) sequence of PLRV. The RT-LAMP reactions were conducted without or with a set of loop primers. By real-time monitoring using Turbimeter, the RT-LAMP (with loop primers) detects PLRV in less than 30 min, compared to 120 min of RT-PCR. By adding fluorescent reagent during the reaction, final products of the RT-LAMP were fluorescently visualized under UV light or could be differentiated by naked-eye inspection under normal light. The RT-LAMP was extremely sensitive, about 2000-fold more sensitive than RT-PCR. This study presents great potential of the RT-LAMP for diagnosis and PLRV epidemiology because RT-LAMP method is speedy, sensitive, inexpensive, and convenient.

• Transactions of Materials Processing
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• v.21 no.3
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• pp.195-201
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• 2012

A vacuum ladling die casting system is suggested as a means to obtain a high vacuum level. A high vacuum of 17.8 mmHg is obtained by sealing the inner space of the mould. The sample product is a rear-head housing for an auto-compressor, and the die-casting with 6-cavities was conducted. The flow analysis shows that the filling speed during vacuum ladling is faster than for a non-vacuum system. The air holes in the sample product were too small to be seen with the naked eye in X-ray films. Density tests show that the high vacuum ladling system reduces the internal porosity as much as 57.8% when compared to the non-vacuum system. A defective rate of only 0.17% was found from leak testing. The results of this research prove that the high vacuum die-casting process is useful for manufacturing of aluminium components under high internal pressure.

• Proceedings of the Korean Institute of Building Construction Conference
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• 2005.05a
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• pp.21-24
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• 2005

In this paper, field application of mass concrete using setting time difference of super retarding agent is reported to reduce hydration heat of concrete placed at newly constructed apartment house in Daejeon. Horizontal placing lift is applied. According to test results,: slump and air content meets the requirement of target values. For compressive strength, it exceeds the nominal strength ordered by the costumer. For temperature history, maximum temperature of center at top section shows $25.6^{\circ}C$, and at bottom section, $35.4^{\circ}C$. According to naked eye's investigation, no hydration heat crack is observed at the surface of concrete.

• Proceedings of the Korean Society of Dyers and Finishers Conference
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• 2011.03a
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• pp.35-35
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• 2011

The design and preparation of novel dye rotaxanes have gained much interest recently, since such structure usually exhibits peculiar spectral and optical changes. In spite of the promising results to date, increasing pressure remains to develop novel supramolecular structures based on stimuli-responsive systems. This presentation covers the study of inclusion complexes of cyclodextrins and various chromophores, with an emphasis on our most recent outcome of anisotropic hydrogel. In this system, physical gelation prepared from simple mixture of CD and a azo dye is completed through specific host-guest interaction. The obtained hydrogel exhibits respective morphological transitions based on supramolecular assembly and dissociation, leading to either precipitation or a sol-to-gel transition. It can identify different classes of metal ions, and, among them, naked-eye differentiation of lead ion is possible due to the coordination-induced unthreading of dye molecules. Accompanying structural changes were verified by numerous characterization techniques, including 2D-ROESY, HR-MAS, UV-Visible absorption, small-angle X-ray scattering, and induced circular dichroism measurements. Such properties discussed here will find useful in analytical applications, such as metal ion sensing and removal applications.

• Proceedings of the Korea Concrete Institute Conference
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• 2004.05a
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• pp.144-147
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• 2004

In this paper, field application of mass concreting using super retarding agent(SRA) are discussed based on setting. time difference with SRA in big discount market in Chongju. Mechanical and physical properties of .concrete are investigated. Temperature history of concrete is also measured. Slump and air content meet the requirement of target value. Compressive strength of concrete exceeded the nominal strength with 24MPa. Compressive strength of SRA concrete is higher than that of plain concrete by about $3\~4\%$. For temperature history, peak temperature of concrete at middle section at top concrete layer reached $49.6^{\circ}C$ within 24hours, and at bottom concrete layer, $54.6^{\circ}C$ within 42hours. Based on the naked eye's observation, no crack was found at mass concrete.

• Tunnel and Underground Space
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• v.5 no.4
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• pp.347-362
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• 1995

The purpose of this study was that manage effectively the excavation process of the transport tunnel in DONGHAE mine area by investigating the variationof the surface and groundwater flow system around the tunnel and neighbouring villages. Thus, the effect of excavation and water-prrofing process on the water system has been studied through the naked eye survey of the tunnel and the surface outcrop, joint survey, core drilling, the measurement of the surface water quantity, evapotranspiration and precipitation analysis, rock hydraulics approach, the pressure test of boreholes, the variation of the water level, and finally the numerical analysis. From above approachs, we derived the conclusion that the exhaustion of the surface water was not caused by the tunnel excavation on the groundwater system was minimized by effective water proofing process.

• The Journal of Korea Institute of Information, Electronics, and Communication Technology
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• v.3 no.1
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• pp.13-18
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• 2010

In this study, heart rate through ECG R-R intervals using the methods about how to automatically extract studied. Heart rate as measured by the naked eye, using the 2-order differential equations to extract heart rate, using self-correlation function to extract the heart rate was compared contemplate. To verify its efficacy and validity in practical applications, these method has been applied to MIT/BIH database. Based on this, making a ECG meter automatic heart rate measurements, and our ECG meter was compared with the existing ICU.

• Textile Coloration and Finishing
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• v.25 no.1
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• pp.18-24
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• 2013

A water soluble spironaphthoxazine (SPO) was synthesized, and its spectral properties were determined. Under UV irradiation, colorless SPO shows intensive blue color while the intensity of its initial fluorescence decreased. In addition, SPO also exhibited high sensitivity to pH stimuli both in colorimetry and fluorometry distinguishing from the spectral appearance observed under UV irradiation. Further, integrating these two optical characteristics a three-state switching system can be established, and all interconversions can be observed by naked-eye.

• The Journal of Korean Medicine
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• v.24 no.4
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• pp.136-138
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• 2003

Purpose : A clinical case of diabetic foot-wound repair using Hirudo medicinalis therapy and its application is reported and discussed. Methods : The subject was a sixty-six-year-old woman diagnosed with a non-healing wound indicating the amputation of her right diabetic foot. Instead, Hirudo medicinalis therapy was performed twelve times over forty days, twice a week. The transition of wound closure was observed with the naked eye, and the plethysmogram was measured at the beginning and end of the treatment period. Results : The wound contracted with decreased exudate, collagen-like connective tissue sprouted, and then the wound gradually closed. The initial value (-0.08) of the dicrotic elastic index rose to -0.03. Conclusion : Hirudo medicinalis therapy had a positive effect on the wound repair, without a negative effect on the circulation, allowing the foot to be saved. It is necessary to investigate its wound repair mechanism for the further development of the Sahyul method.

• Proceedings of the Korea Concrete Institute Conference
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• 2005.11a
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• pp.121-124
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• 2005

The existing reinforcement methods of construction are the actual condition without the method of checking exact injection of adhesives clearly by the opacity of reinforcement material. In this study, in order to solve such a problem, the high strength transparent panel using a glass fiber is developed and in order to examine reinforcement effect of a panel clearly, the structure performance evaluation experiment was conducted. As a result, we knew that it can check with the naked eye the injection process of adhesives and reinforcement effect was also observable.