Journal of The Korean Society of Grassland and Forage Science
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v.6
no.1
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pp.6-13
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1986
Experiments were conducted to study the effects of formic acid on the quality of the rye silages at the different dry matter content (19.2, 28.6 and 32.2%) and different formic acid addition levels (0.00, 0.24, 0.48 and 0.71%). Rye were taken at vegetative stage (plant height 40cm) on November 29, 1984. Herbage were adjusted dry matter contents by wilting. Materials were ensiled in small polyethelene film bag after addition planned formic acid, and stored under room temperature. The results obtained are as follows: 1. In the visual observation of silage quality by addition of formic acid in unwilted silage show more clear brownish-yellow color and sweet flavor and less acidity compared with untreated formic acid. 2. The pH values of the silage increased by increasing DM content. At lower dry matter contents in materials the pH values decreased with increased formic acid levels. At higher dry matter content silage, however, non significant difference were observed. 3. The formation of total organic acid decreased by increasing DM content in the materials, and there appeared a significant difference (P<01) among formic acid levels. The organic acid contents in silage decreased with higher formic acid levels. 4. The water soluble carbohydrate in silage increased by increasing DM content. And it was also increased (P<01) with increased formic acid addition level. 5. The production of NH_3 - N decreased (P<01) with increasing the addition of formic acid, and decreased by increasing DM content in materials. 6. The in vitro dry matter digestibility of silages showed not difference among dry matter contents of the materials but at 0.71% addition of formic acid in unwilted silages appeared higher (P<05) digestibility compared the others (0.00% and 0.24%).
Journal of The Korean Society of Grassland and Forage Science
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v.27
no.4
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pp.287-296
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2007
The experimental work was conducted to determine the growth characteristics and yield of whole crop rice (cv. Suwon 468 and cv. Chuchungbeo) and soil properties using various type of livestock manure application on rice paddy land for 3 years ($2003{\sim}3005$). Compared Suwon 468 and Chuchungbeo, Suwon 468 has longer plant height and more DM yield than that of Chuchungbeo. Among livestock manure type, plant height was longer in order of liquid swine manure (LSM) > composted swine manure (CSM) > chemical fertilizer (CF) > composted cattle manure (CCM). Number of branch on Chuchungbeo had more than that of Suwon 468. Among livestock manure type, number of branch had more in order of LSM > CSM > CF > CCM. DM yield of whole crop rice (WCR) was affected by various types of livestock manure application and increased in order CSM > CCM = LSM. DM yield on the effects of application level of LSM was highest in LSM 75% + CF 25%. Plant diseases such as rice blast, damage by insect, smut, sheath blight occurred in LSM and CSM and there was not significantly different among application level of LSM. The nitrogen content of WCR by CSM was the highest of all treatments and the ripened ratio by CSM was in contrary order. Moreover the feed value of WCR was not significantly different among treatments. Soil pH, organic matter and total nitrogen was not different by LSM application whereas phosphorus content increased by LSM application. Cu and Zn content increased by LSM and CSM application and were not different by CCM as compared to control plots.
In order to observe the lasting effect of NK-compound mineral fertilizer and organic compound fertilizer including Myweon organic liquid fertilizer, a pot experiment was conducted with rice (Oriza Sativa) variety: Nong Back. These fertilizers were applied as basal and the same amount of urea form of nitrogen was top dressed about a month after transplanting, July seventh, 1977. Results obtained are as follows; 1. The lasting effect of various fertilizers were laid in following decreasing order; Myweon liquid < Organic compound fertilizer (Myweon coop. made) > NK-compound fertilizer (Chosun coop. made) Single fertilizer. It was considered that organic matter served as microbial feed and lead a temporary fixation of available plant nutrients in the soil, and the reduced surface area of the compound fertilizers slowed down the availability of the fertilizers. 2. The fertilizer showing greater lasting effect produced more panicles per hill and less grains per panicle than the fertilizers showing less lasting effect, and brought low maturity, which resulted in low paddy yield the paddy producing efficiency of nitrogen absorbed by straw was also low in the former fertilizers. Such advanced effect of the former fertilizer was considered to be related with the variety of early maturity and unseasonable topdressing of fertilizer which made at the maximum tillering stage. 3. For the production of Japonica type paddy with heavy fertilization which may required to depress the early growth a little and promote the late growth, it might be necessary to develop slow releasing fertilizers such as single fertilizer formulated to a large grains or compound fertilizer containing organic matter. 4. If the nitrogen content of paddy, Nong Back, far excess 0.64 or 0.65% and reaches 0.68% or above, the yield of the variety seemed to be decreased remarkbly through the low maturity rate and thousand grain weight.
Eighty five patients of oral cavity cancer, treated with radiation at the Department of Therapeutic Radiology, Korea Cancer Center Hospital, during the period from March 1985 to September 1990 were analyzed retrospectively. Among 85 patients, 37 patients were treated with radiation only and 48 patients were treated with radiation following surgery. And 70 patients received external irradiation only by $^{60}Co$ with or without electron, the others were 7 patients for external irradiation plus interstitial implantation and 8 patients for external irradiation plus oral cone electron therapy. Primary sites were mobile tongue for 40 patients, mouth floor for 17 patients, palate for 12 patients, gingiva including retromolar trigone for 10 patients, buccal mucosa for 5 patients, and lip for 1 patient. According to pathologic classification, squamous cell carcinoma was the most common (77 patients). According to AJC TNM stage, stage I + II were 28 patients and stage III+IV were 57 patients. Acturial overall survival rate at 3 years was $43.9\%,$ 3 year survival rates were $60.9\%$ for stage I + II, and $23.1\%$ for stage III+IV, respectively. As a prognostic factor, primary T stage was a significant factor (p<0.01). The others, age, location, lymph node metastasis, surgery, radiation dose, and cell differentiation were not statistically significant. Among those factors, radiation plus surgery was more effective than radiation only in T3+T4 or in any N stage although it was not statistically sufficient (p<0.1). From those results, it was conclusive that definitive radiotherapy was more effective than surgery especially In the view of pertainig of anatomical integrity and function in early stage, and radiation plus surgery was considered to be better therapeutic tool in advanced stage.
Suh Chang Ok;Chung Eun Ji;Lee Hy De;Lee Kyong Sik;Oh Ki Keun;Kim Gwi Eon
Radiation Oncology Journal
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v.15
no.4
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pp.331-337
/
1997
Purpose : To evaluate our experience in the breast-conserving treatment for early breast cancer with special regard to recurrence Pattern and related risk factors. Materials and Method :Two hundred and sixteen patients with AJC stage 1and 11 breast cancer who received breast conserving treatment between January 1991 and December 1994 were evaluated A9e distribution ranged from 23-80 year old with a median a9e of 44. One hundred and seventeen Patients had 71 lesions and 99 Patients had 72 lesions. Axillary lymph nodes were involved in 73 patients. All patients received a breast conserving surgery (wide excision to quadrantectomy) and axillary node dissection followed by radiotherapy. Ninety six patients received chemotherapy before or after radiotherapy. Results : During the follow-up period (3-60 months, median 30 months), local recurrences were noted in six Patients (true: 3, elsewhere; 1, skin: 2). Sixteen patients developed distant metastases as the first sign of recurrence at 8-38 months (median 20 months) after surgery. Among them, three patients simultaneously developed local recurrence with distant metastases. Contralateral breast cancer developed in one Patient and non-mammary cancers developed in three patients. The actuarial 5 year survival rate was $88.4\%\;(stage\;I\;96.7\%,\;stage\;IIa\;95.2\%,\;stage\;IIb\;69.9\%)$. Age, I stage, number of involved axillary lymph nodes. and AJC stage were risk factors for distant metastases in univariate analysis. In the multivariate analysis, the number of involved axillary lymph nodes was the most significant risk factor for metastases. Conclusion : Local recurrence was not common in the early years after radiotherapy. Distant metastases occurred at a steady rate during the first three years and was more common in the Patients with larger tumors, higher number of involved axillary nodes, and younger age.
Purpose : The aim of this study was to determine the risk factors, clinical characteristics and prognosis for the development of periventricular leukomalacia (PVL) in preterm infants according to the extent and site of the PVL. Methods : The medical records of infants (under 36 weeks of gestational age) delivered from January 1999 to December 2008 were reviewed. Twenty-five preterm infants with were PVL were diagnosed by brain magnetic resonance imaging (MRI) and an addition 50preterm infants with no brain lesions were enrolled in this study. The perinatal and neonatal risk factors for the development of PVL was determine in these infants. Mental and Psychomotor Developmental Indices (MDI, PDI) were assessed by a clinical psychologist using the Bayley Scales of Infant Development II. We compared the differences of the clinical characteristics and prognosis according to brain MRI findings. Results : Maternal fever, young maternal age, extended oxygen use, hypotension within the first week of birth, use of inotropics within the first week of birth, and respiratory distress syndrome were the risk factors associated with PVL (P <0.05). In the multivariate analysis, maternal fever and extended oxygen use were statistically significant independent risk factors (P <0.05). The mean MDI and PDI scores of the PVL group (74.4$\pm$ 27.8 and 58.0$\pm$17.7) were significantly lower than those of the control group (103.5$\pm$8.9 and 101.7$\pm$16.1, P <0.05). Conclusion : Maternal fever and extended oxygen use were independent risk factors for PVL. We should pay attention to infants who had the risk factors and follow them up closely by brain imaging study and Bayley Scales of Infant Development II.
Purpose : This study was performed to determine the predictors of failed closure of a patent ductus arteriosus (PDA) following the first course of indomethacin in symptomatic preterm infants. Methods : Forty three of 43 preterm infants, admitted to the neonatal intensive care unit diagnosed with PDA and treated with indomethacin at the Korea University Medical Center between January 1990 and October 2007, ware studied. The perinatal risk factors affecting the failed closure of PDA were retrospectively assessed. Results : The failed PDA closure group included 16 (37%) out of 43 infants three of whom underwent surgery. The closure group included 27 (63%) out of 43 infants. In the failed closure group, the Apgar scores (1 min, 5 min) were significantly higher (P<0.05) and antenatal steroid administration was significantly lower (P<0.05). In addition, dopamine administration was significantly lower (P<0.05) and the mean postnatal age at diagnosis was significantly lower (P<0.05). Multiple logistic regression for the prediction of failed PDA closure found only antenatal steroid administration (OR 0.092, CI 0,010-0.826, P=0.0331) as an associated factor. Conclusion : In patients with antenatal steroid administration the failed PDA closure rate was significantly lower. Therefore, antenatal steroid administration can be considered as an important factor for the closure of PDA in preterm pregnancies.
The previous monoclonal antibody labeling method for bone marrow immunoscintigrapy was complicated and laborious for clinical application. Also it showed a relatively low labeling efficiency. To improve this procedure, we compared several direct labeling methods of $^{99m}Tc$. 1) The labeling efficiency in the method using gluconate as a transchelator was low (40-70%), but immunoscintigraphy using this radiotracer produced a clear image. 2) To improve labeling efficiency, ${\beta}$-mercaptoethanol was removed after reduction. The labeling efficiency was improved up to 70-80%, but the radioactivity of the blood pool was high. 3) The higest labeling efficiency (>90%) and best quality images could be obtained by using MDP as a transchelating agent. It did not require additional procedures for separation of labeled antibodies. The immunoreactivity of this antibody was 60%. Residual MDP which can be taken up by the bone could be removed by PD-10 column. The reduced antibodies were stable with a high labeling efficiency (>90%) for up to 47 days by deep freezing. We concluded that the improved procedure for $^{99m}Tc$ labeling of anti-NCA-95 monoclonal antibody using MDP as a transchelating agent will be a simple and useful method for clinical application.
OH, IM SANG;RABINOVICH, ALEXANDER B.;PARK, MYOUNG SOOK;MANSUROV, ROALD N.
한국해양학회지
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v.28
no.1
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pp.1-16
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1993
The monthly mean sea levels at 48 stations located at the East and Yellow Seas coasts of Korea, Russia and Japan are processed to investigate seasonal sea level variations. The strong seasonal variations are found to be at the west coast of Korea (42.1 cm in Kunsan), in the region of the Korea strait and near the southern part of Primorye (30-33 cm); the weak ones near the southwestern coast of the Sakhalin Island (10-12 cm). Practically for the whole study area except the southwest Sakhalin, the general picture of the seasonal sea level changes is alike: the mean sea level rises in summer-autumn and falls in winter-spring. The spectral analysis of the records also shows that the seasonal oscillations strongly dominate in the sea level variations, more than 80% or total energy in the southern part of the investigated region and 50-70% in the northern part relate to these oscillations. The annal peak significantly prevails in spectra of the monthly sea levels for the majority of stations, the semiannual peak is also well manifested, but the seasonal peaks of higher order (corresponding to the periods of four and three months) reveal only at some records. The maximal amplitudes of annual component by a least square method are found at the Yellow Sea coast of Korea (20-21 cm) and also near the Japanese coast of the korea Strait (19-19 cm). The semiannual component has the maximal amplitudes (3-4 cm) near the south and southwestern coasts of the Sakhalin Island. The annual range of the sea levels is much weaker here than in the other regions, the relative investment of the seasonal oscillations in total energetic budget is only 35-40%, annual ($A_1$) and semiannual ($A_2$) components have nearly the same amplitude (seasonal factor $F=A_1/A_2=0.9-1.2$). On the basis of the present examination on sea level changes together with the results of Tomizawa et. al.(1984) the whole investigated area may be divided into 10 subregions, 2 of them are related to the Yellow Sea and Western part of the Korea Strait (Y1, Y2), the other ones (E1-E8) to the East Sea.
The purpose of this study was to investigate the effects of embryo sources such as in vivo vs. in vitro produced blastocyst, and culture systems on the membrane permeability and viability of bovine blastocyst following GMP vitrification. To produce in vivo embryos, six cows were superovulated by administration of follicle stimulation hormone (FSH) and prostaglandin $F_{2{\alpha}}$(PG $F_{2{\alpha}}$). in vitro embryos were produced by two different culture systems, oviduct co-culture (OCS) and defined culture system (HECM-6; DCS). Ovaries were picked up at a local slaughterhouse and transported to laboratory in 3$0^{\circ}C$ saline within 2 h. Ovaries were washed with same saline three times and then placed in saline on warm plate adjusted at 3$0^{\circ}C$ during aspiration. The blastocysts produced were assigned for membrane permeability and viability following GMP vitrification. The membrane permeability of blastocysts was checked in 0.5 M sucrose solution on warm plate at 35$^{\circ}C$ for 0, 2, 5 and 7 min, respectively. Then the diameters (width and length) of embryo cytoplasms were measured by a eyepiece meter, and they were converted to their volume by 4/3 $\pi$$r^3$. The blastocysts were cryopreserved by GMP vitrification method, where they were sequentially placed into vitrification solution before being loaded into GMP vessels and immersed into L$N_2$ within 20 to 25 sec. Post-thaw blastocysts were serially washed in 0.25 and 0.15 M sucrose in HM and TCM-199 for 5 min each, and then cultured in TCM 199 supplemented with 10% FCS for 24 or 48 h. The volume change of in vivo blastocyst at 0, 2, 5 and 7 min (100, 37.1, 34.3 and 31.6%) was significantly more shrunk than those of in vitro blastocysts derived from OCS (100, 59.8, 48.9, 47.9%) and DCS (100, 57.2, 47.3 and 46.9%) (P<0.05). The viability of post-thaw blastocyst derived from in vivo (93.6%) was also significantly different from those in OCS and DCS (81.9 and 83.6%; P<0.05). In the present culture system, the morphology of embryos produced in vitro was similar to that of in vivo embryos, but the quality in membrane permeability and post-thaw viability showed a big difference from their sources as in vivo or in vitro derived from OCS and DCS. The results indicated that the quality of in vivo embryos in membrane permeability and post-thaw viability was better than those of in vitro embryos derived from OCS or DCS.
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