• Journal of the Society of Cosmetic Scientists of Korea
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• v.46 no.3
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• pp.253-263
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• 2020

Reactive oxygen species (ROS) plays an important role in maintaining homeostasis. However, excessive ROS production damages cellular components such as proteins, lipids, and nucleic acids and promotes skin aging. In this study, we confirmed the antioxidant effect of CSYJE to prevent excessive oxidative stress. First, DPPH and ABTS assays were performed to confirm the antioxidant effect of CSYJE and the radical scavenging activity was confirmed depending on the concentration. As a result of performing the MTT assay to confirm the cell viability, it was confirmed that there was no cytotoxicity at a concentration of 1,000 ㎍/mL. As a result of western blotting to confirm the expression levels of the antioxidant-related proteins nuclear-E2-related factor 2 (Nrf2) and Heme oxygenase-1 (HO-1), it was confirmed that the expression was increased in a concentration-dependent manner. After inducing ROS with lipopolysaccharide (LPS), an intracellular ROS-causing substance, DCF-DA was performed to confirm the inhibitory effect of ROS production, and the inhibition of ROS production was confirmed to concentration-dependent. Real-time RT-PCR was performed to confirm the mRNA expression level of inflammatory cytokines and inflammatory mediator caused by ROS generation, mRNA expression was reduced in a dose dependent manner. Therefore, this study confirmed the antioxidant effect of CSYJE through the Nrf2/HO-1 signaling pathway, which suggests that CSYJE can be used as an antioxidant cosmetic material by inhibiting free radicals.

• Journal of Ginseng Research
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• v.11 no.2
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• pp.136-144
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• 1987

Biosynthesis of saponins from acetate, mevalonate and squalene using root slices of panax ginseng C.A. Meyer was investigated. The sliced roots (2g) were incubated with the reaction mixture containing 20 M sodium acetate ($500\mu$Ci [U-$^{l4}C$]-acetate),10 mM mevalonate ($25\mu$Ci [2-$^{l4}C$]-mevalonate) or 10 mM swidme ($10\mu$Ci [4,8,12,13,17,21-$^3H$]-squalene) respectively at $30^{\circ}C$ for 72 hours. Biosynthesis of labelled ginseng saponine from [U-l4C]-acetate, [2-$^{l4}C$]-mevalonate and [4,8,12,13,17,21-$^3H$]-squalene was confirmed by autoradiography. Analysis of the products from [U-$^{l4}C$]-acetate by T.L.C. showed that the % radioactivities in panaxadiol, panaxatriol, squalene and mevalonate were found to be 2.1%, 2.7%, 2.6% and 0.2% respectively. Some of the sugars were also highly labelled. Analysis of the products from [2-$^{l4}C$]-mevalonate by T.L.C. showed that squalene was highly labelled and the products from [4,8,12,13,17,21-$^3H$]-squalene showed that panaxadiol, panaxatriol and sterol were highly labelled. From the above results, it was suggested that saponine might be synthesized from acetate via mevalonate-squalene route as expected in ginseng root.

• Proceedings of the KSR Conference
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• 2007.11a
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• pp.1731-1737
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• 2007

This paper considers subway routing problem. Given a schedule of train to be routed by a railway stock, the routing problem determines a sequence of trains while satisfying turnaround time and maintenance restrictions. Generally, the solution of routing problem is generated from set partition formulation solved by column generation method, a typical integer programming approach for train-set. However, we find the characteristics of metropolitan subway which has a simple rail network, a few end stations and 13 departure-arrival patterns. We reflect a turn-around constraint due to spatial limitations has no existence in conventional railroad. Our objective is to minimize the number of daily train-sets. In this paper, we develop two basic techniques that solve the subway routing problem in a reasonable time. In first stage, we formulate the routing problem as a Min-cost-flow problem. Then, in the second stage, we attempt to normalize the distance covered to each routes and reduce the travel distance using our heuristic approach. Applied to the current daily timetable, we could find the subway routings, which is an approximately 14% improvement on the number of train-sets reducing 15% of maximum traveling distance and 8% of the standard deviation.

• Journal of Institute of Control, Robotics and Systems
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• v.12 no.7
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• pp.671-678
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• 2006

This article proposes a smooth path generation and time scheduling method for general tasks defined by non-smooth path segments in industrial robotic applications. This method utilizes a simple 3rd order polynomial function for smooth interpolation between non-smooth path segments, so that entire task can effectively maintain constant line speed of operation. A predictor-corrector type numerical mapping technique, which correlates time based speed profile to the smoothed path in Cartesian space, is also provided. Finally simulation results show the feasibility of the proposed algorithm.

• Journal of the Korean Society for Precision Engineering
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• v.16 no.10
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• pp.126-131
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• 1999

Up until now, it is said that no satisfactory computer solutions have been found for synthesizing four-bar linkage based on the prescribed coupler link curve. In our study, an algorithm has been developed to improve the design synthesis of four-bar linkage automatically generating prescribed path by using B-spline interpolation. The suggested algorithm generates the desired coupler curve by using B-spline interpolation, and hence the generated curve approximates as closely as to the desired curve representing coupler link trajectory. Also, when comparing each generated polygon with the control polygon, rapid comparison by applying convex hull concept. finally, optimization process using ADS is incorporated into the algorithm based on the 5 precision point method to reduce the total optimization process time. As for examples, three different four-bar linkages were tested and the results showed the effectiveness of the algorithm.

• Journal of the Korean Society of Manufacturing Technology Engineers
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• v.18 no.6
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• pp.576-584
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• 2009

Up to now, it has been said that no satisfactory computer solution has been found for synthesizing four-bar linkage based on the prescribed coupler link curve. In our study, an algorithm has been developed to improve the design synthesis of four bar linkage based on the 5 precision points method. The suggested algorithm generates the desired coupler curve by using NURBS, and then the generated curve approximates as closely as possible to the desired curve representing coupler link trajectory. Also, when comparing each generated curve by constructing the control polygon, rapid comparison is easily achieved by applying convex hull of the control polygon. Finally, an optimization process using ADS is incorporated into the algorithm based on the 5 precision point method to reduce the total optimization process time. As for examples, two four bar linkages were tested and the result well demonstrated the effectiveness of the algorithm.

• Tuberculosis and Respiratory Diseases
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• v.56 no.2
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• pp.187-197
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• 2004

Background : Nitric Oxide (NO) is a multi-faceted molecule with dichotomous regulatory roles in many areas of biology. NO can promote apoptosis in some cells, whereas it inhibits apoptosis in other cell types. This study was performed to characterize NO-induced cell death in lung epithelial cells and to investigate the roles of cell death regulators including iron, bcl-2 and p53. Methods : A549 cells were used for lung epithelial cells. SNP (sodium nitroprusside) and SNAP (S-nitroso-N-acetyl- penicillamine) were used for NO donor. Cytoxicity assay was done by MTT assay and crystal violet assay. Apoptotic assay was done by fluorescent microscopy after double staining with propidium iodide and hoecst 33342. Iron inhibition study was done with RBCs and FeSO4. For bcl-2 study, bcl-2 overexpressing cells (A549-bcl-2) were used and for p53 study, Western blot analysis and p53 functionally knock-out cells (A549-E6) were used. Results : SNP and SNAP induced dose-dependent cell death in A549 cells and fluorescent microscopy revealed that SNAP induced apoptosis in low doses but necrosis in high doses while SNP induced exclusively necrotic cell death. Iron inhibition study using RBCs and FeSO4 significantly blocked SNAP-induced cell death. And also SNAP-induced cell death was blocked by bcl-2 overexpression. Finally, we found that SNAP activate p53 by Western blot analysis and that SNAP-induced cell death was decreased in the abscence of p53. Conclusion : In lung epithelial cells, NO can induce cell death, more precisely apoptosis in low doses and necrosis in high doses. And iron, bcl-2, and p53 play important roles in NO-induced cell death.

• Korean Journal of Plant Resources
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• v.31 no.4
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• pp.294-302
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• 2018

This study was conducted to compare anti-inflammatory effect of Robinia pseudoacacia L. using different extraction methods (water extraction, ethanol extraction and high temperature extraction). We investigated anti-inflammatory effect of Robinia pseudoacacia L. extract (RP1, water extract; RP2, ethanol extract; RP3, high temperature extract) on lipopolysaccharide (LPS)-stimulated inflammation using Raw 264.7 cell. Cells were treated with various concentrations (12.5, 25, 50, 100 or $200{\mu}g/m{\ell}$) of water extract, ethanol extract and high temperature extract. Cytotoxicity was not observed on Raw 264.7 cells, LPS-stimulated production of NO (nitric oxide), $PGE_2$ (prostaglandin $E_2$) and cytokines ($TNF-{\alpha}$, IL-6 and $IL-1{\beta}$) was reduced by RP3 treatment more than RP1 and RP2. In conclusion, these results indicated that inflammation on Raw 264.7 cells was improved by RP3. Treatment of RP3 could be used to natural medicine for improving inflammatory response. However, further experiment is required to observe how the high temperature extraction at $500^{\circ}C$ for 48 h influences on alteration of active ingredient in Robinia pseudoacacia L., and conducts the inflammation signal pathway on Raw 264.7 cells.

• Journal of the Institute of Electronics and Information Engineers
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• v.53 no.4
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• pp.68-75
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• 2016

As the number of cores and IPs increase in multiprocessor system-on-chip (MPSoC), network-on-chip (NoC) has emerged as a promising novel interconnection architecture for its parallelism and scalability. However, minimization of the latency in NoC with legacy bus IPs must be addressed. In this paper, we focus on the latency minimization problem in NoC which accommodates legacy bus protocol based IPs considering the trade-offs between hop counts and path collisions. To resolve this problem, we propose separated address/data network for independent address and data phases of bus protocol. Compared to Mesh and irregular topologies generated by TopGen, experimental results show that average latency and execution time are reduced by 19.46% and 10.55%, respectively.

• Journal of KIISE:Computing Practices and Letters
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• v.15 no.12
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• pp.938-942
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• 2009

In this paper, we propose a novel filtering method for processing continuous skyline queries on wireless sensor network environments. The existing filtering methods use the filter based on router paths. However, because these filters are applied not to a whole area but to a partial area, these methods send almost data of sensor nodes to transmit to the base station and have no sufficient effect in terms of energy efficiency. Therefore, we propose an efficient method to dramatically reduce the transmission data of sensors through applying a low-cost and effective filter to all sensor nodes. The proposed effective filter is generated by using characteristics such as the data locality and the clustering of sensors. An extensive performance study verifies the merits of our new method.