• Journal of Life Science
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• v.20 no.4
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• pp.467-473
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• 2010

Gangliosides are a major component of the plasma membrane of mammalian cells, which are directly involved in a variety of immunological events, including cell-to cell or cell-to-protein interactions. In this study, we investigated whether gangliosides, sialic acid-containing glycosphingolipids, are related to rejection during the xenotransplantation of NIH-miniature pig livers and hearts to humans. Both high performance thin-layer chromatography and immunohistochemistry analyses revealed that the expression of gangliosides in the liver tissue of NIH-miniature pigs was higher than that in the heart. Gangliosides GD3, GD1a, GD1b, GT1b and GQ1b were observed in both the liver and heart, whereas GQ1b was detected only in the liver, indicating that the ganglioside expression profiles are tissue specific. Moreover, other ganglio-series gangliosides, including GM3, were not detected in the livers and hearts of NIH-miniature pigs. Taken together, these results suggest that gangliosides may play important roles in immune responses in clinical xenotransplants of pig livers and hearts.

• Asia pacific journal of information systems
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• v.25 no.2
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• pp.306-328
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• 2015

A recent trend in innovation paradigm is the shift from closed innovation to open innovation principles. This trend is characterized by the use of purposive inflows and outflows of knowledge not only to accelerate internal innovation, but also to expand the markets for external use of innovation. However, the Not-Invented-Here (NIH), and Not-Sold-Here (NSH) or Only-Use-Here (OUH) syndromes are the most common challenges encountered in open innovation processes. Therefore, this study has the following main aims: 1) propose a research model that explains the factors that influence NIH and NSH/OUH attitudes based on motivation theory with perceived organizational fairness and commitment, 2) investigate their impacts on NIH and NSH/OUH attitudes, and 3) provide useful theoretical and practical insights. The proposed research model was empirically tested using survey data collected from information technology (IT) professionals in Korea. One of the major findings indicates that IT professionals' perceived organizational fairness has a negative effect on NIH attitudes in open innovation process. Theoretical and practical contributions of this study are also discussed.

• Korean Journal of Veterinary Research
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• v.30 no.4
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• pp.459-464
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• 1990

Inhibitory effect of caffeine on NIH3T3 cell proliferation was studied by using [$^3H$]thymidine incorporation and a modified one-step silver staining technique. The latter technique shows argyrophilic nucleolar organizer region-associated proteins (Ag-NORs), which are seen in nuclei as black dots. The result was compared with the counts of [$^3H$] thymidine incorporation. The results were summarized as follows; 1. The Ag-NORs numbers of NIH3T3 cells were $6.81{\pm}1.38$ at 24 hrs, $7.13{\pm}1.26$ at 48 hrs, $8.50{\pm}2.04$ at 72 hrs after incubation. 2. The numbers of Ag-NORs were significantly decrease in caffeine treated groups (p<0.01). 3. The counts of [$^3H$] thymidine incorporation were similar to the result of using Ag-NORs staining technique. It is concluded that Ag-NOR is a rapid, simple and compatible method to quantitate cell proliferation as compared with [$^3H$]thymidine incorporation.

• Molecules and Cells
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• v.27 no.3
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• pp.299-305
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• 2009

Over-expression of phospholipase D (PLD) 1 or PLD2 down-regulated CKII activity in NIH3T3 cells. The same results were found with catalytically inactive mutants of PLD isozymes, indicating that the catalytic activity of PLD is not required for PLD-mediated CKII inhibition. Consistent with this, 1-butanol did not alter CKII activity. The reduction in CKII activity in PLD-over-expressing NIH3T3 cells was due to reduced protein level, but not mRNA level, of the $CKII{\beta}$ subunit. This PLD-induced $CKII{\beta}$ degradation was mediated by ubiquitin-proteasome machinery, but MAP kinase and mTOR were not involved in $CKII{\beta}$ degradation. PLD isozymes interacted with the $CKII{\beta}$ subunit. Immunocytochemical staining revealed that PLD and $CKII{\beta}$ colocalize in the cytoplasm of NIH3T3 cells, especially in the perinuclear region. PLD binding to $CKII{\beta}$ inhibited $CKII{\beta}$ autophosphorylation, which is known to be important for $CKII{\beta}$ stability. In summary, the current data indicate that PLD isozymes can down-regulate CKII activity through the acceleration of $CKII{\beta}$ degradation by ubiquitin-proteasome machinery.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.20 no.1 s.32
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• pp.145-153
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• 2007

Objecgtive : The aim of present study was to investigate inhibition effect of Whakijogyung-Tang(WJT) on the tumor cell lines. This study estimated the cytotoxicity of WJT about viability of S-180 and NlH3T3. Methods : The cytotoxicity of WJT about viability of cells were tested using a colorimetric tetrazoliun assay(MTT assay) Results and Conclusion : 1. Water extract of WJT had $IC_{50}$ of 863 ${\mu}g/ml$ in S-180 cell lines, but cytotoxicity of NIH3T3 was not significant difference compare with S-180. 2. n-Hexane fraction of WJT had similar cytotoxicity between S-180 and NIH3T3, but that could not have $IC_{50}$ in S-180 cell lines. 3. Ethyl acetate fraction of WJT had low degree cytotoxicity both S-180 and NIH3T3 cell lines. 4. Significantly, Butanol fraction of WJT had differenct citotoxicity between S-180 and NIH3T3. 5. $H_2O_2$ fraction of WJT had no cytotoxicity both S-180 and NIH3T3.

• The Journal of Korean Medicine
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• v.22 no.3
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• pp.98-104
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• 2001

Objective : In order to study the effect of contralateral both side acupuncture on recovery of motor disorders in stroke patients, a clinical study was performed. Methods : Thirteen patients with post-stroke hemiplegia were randomized into two groups. Six patients (test group) were treated with contralateral both side acupuncture. The other seven patients (control group) were treated with ipsilateral both side acupuncture. The activity of daily living was measured with a National Institutes of Health (NIH) scale. The therapy was performed once a day for 3 weeks. Results : In terms of score of NIH, the test group showed statistically meaningful increase after I weeks treatment, while the control group showed statistically meaningful increase after 2 weeks (P<0.05). The results showed no statistically meaningful difference after 3 weeks treatment between the groups. Conclusions : These results support that contralateral both side acupuncture therapy has almost the same effectiveness compared with ipsilateral both side acupuncture therapy in improvement of the activity of daily living of post-stroke hemiplegic patients.

• Proceedings of the Zoological Society Korea Conference
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• 1999.10b
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• pp.230.2-230
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• 1999

No Abstract, See Full Text

• The Journal of Natural Sciences
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• v.15 no.1
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• pp.89-95
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• 2005

JSRV, which causes sheep lung cancer, is known to have the transforming activity of NIH3T3 cells. Especially Envelope protein of this virus has the transforming activity to NIH3T3. To know the effects on the transcriptional activity of transcription factors by this viral protein transient transfection was performed by using the luciferase reporter system. The result showed that JSRV Envelope protein increased the transcriptional activity of NF-kB and AP-1.

• Proceedings of the Korean Society of Toxicology Conference
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• 2002.05a
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• pp.81-81
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• 2002

The recent development of cDNA microarray or cDNA chip technology has made it possible to analyze the expression of thousands of genes at once. In present study, we made radioresistant clones (#1 and #4) from NIH3T3 cells which are not tumorigenic and we identified 4 genes using microarray system, cdk6, cdc25B, mdm-2 and nidogene, which were altered in radiaiton resistanct NIH3T3 cells.(omitted)

• Proceedings of the Zoological Society Korea Conference
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• 1997.10b
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• pp.308.1-308
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• 1997

No Abstract, See Full Text