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Occurrence and Biological Control of Postharvest Decay in Onion Caused by Fungi

  • Lee, Joon-Taek;Bae, Dong-Won;Park, Seun-Hee;Shim, Chang-Ki;Kwak, Youn-Sig;Kim, Hee-Kyu
    • The Plant Pathology Journal
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    • v.17 no.3
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    • pp.141-148
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    • 2001
  • Postharvest decay of onion bulbs was examined by inspecting the commercial packages in the market or in storage. Bulb rot incidence was unexpectedly high, and onion bulbs with 1st quality grade were rotten most severely by 51%, followed by 32% for 2nd and 21% for 3rd grades. This indicates that larger bulbs had higher incidences of bulb rots. Major pathogens associated with basal and neck rots were Fusarium oxysporum and Aspergillus sp. or Botrytis allii, respectively, of which basal rot was most prevalent and damaging during storage. Among the epiphytic microorgani는 from onion plants, several Bacillus and Paenibacillus spp. and previously selected Pseudomonas putida and Trichoderma harzianum had inhibitory efficacy against bulb rot pathogens. Among these B. amyloliquefaciens BL-3, Paenibacillus polymyxa BL-4, and P. putida Cha 94 were highly inhibitory to conidial germination of F. oxysporum and B. allii. P. putida Cha 94, B. amyloliquefaciens BL-3, P. polymyxa BL-4, and T. harzianum TM were applied in the rhizoplane of onion at transplanting. Initially antagonist populations decreased rapidly during the first one month. However, among these antagonists, rhizoplane population densities of BL-3, Cha 94, and TM were consistently high thereafter, maintaining about 10$^4$-10$^{5}$ cells or spores per gram of onion root up to harvest time. The other bacterial antagonist BL-4 survived only for two months. TM was the most effective biocontrol agent against basal rot, with the number of rotten bulbs recorded at 4%, while that of the control was 16%. Cha 94 was effective for the first 20 days, but basal rot increased thereafter and had about the same control efficacy as that of BL-3 and BL-4. When the antagonists were applied to the topping areas of onion bulbs at harvest, TM was the most effective in protecting the stored onion bulbs from neck rotting. The second effective antagonist was BL-3. TM and BL-3 completely suppressed the neck rot in another test, suggesting that biocontrol of postharvest decay of onion using these microorganisms either at the time of transplanting or at harvesting may be promising.

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Effect of Glutinous Rice Paste on the Fermentation of Puchukimchi (부추김치의 발효숙성에 찹쌀풀이 미치는 영향)

  • 장명숙;박문옥
    • Korean journal of food and cookery science
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    • v.14 no.4
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    • pp.421-429
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    • 1998
  • The effect of glutinous rice paste on the physicochemical, microbiological, and sensory characteristics of Puchukimchi during fermentation was investigated. Puchukimchi is a kind of korean kimchi made with Leek (Allium odorum L.). Puchukimchi made with various concentrations (0.0, 0.5, 1.0, 2.0, 3.0%) of glutinous rice paste were fermentated at 10$^{\circ}C$ up to 45 days. As a fermentation progressed, pH decreased in all of the treatments, while total acidity increased. By increasing the concentrtion of glutinous rice paste added, an increase of total acidity was noted from Puchukimchi. The reducing sugar content in most samples was decreased during fermentation, however, 1.0% treatment showed a relatively slow decrease. The total vitamin C content increased sharply at the 2nd-day of fermentation and then, gradually decreased especially from the 8th-day following a sigmoidal changing pattern. The total vitamin C content was the highest in Puchukimchi made with 1.0% glutinous rice paste. The counts of lactic acid bacteria increased remarkably and then decreased gradually after optimum ripening period, i.e., the palatable period of Puchukimchi during fermentation.

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Synthesis of Hectorite by Hydrothemal Method (저온 수열법에 의한 헥토라이트 합성)

  • Jang, Young-Nam;Chae, Soo-Chun;Ryu, Gyoung-Won;Kim, You-Dong;Jang, Hee-Dong;Bae, In-Kook
    • Journal of the Mineralogical Society of Korea
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    • v.20 no.1 s.51
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    • pp.1-6
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    • 2007
  • Hectorite was synthesized by a two-step hydrothermal process from $Mg(OH)_{2}$, water glass (${\sim}30\;wt%\;SiO_{2}$) and Li-compound at $90{\pm}5^{\circ}C$. The product shows excellent dispersion and swelling properties. The mixture of the starting materials was heated in a glass vessel for the first reaction with continuous stirring and the pH of the solution was adjusted to $6{\sim}8$, resulting in the formation of a precursor of hectorite. The excess salt components were washed out from the resulting slurry and then was matured in the glass vessel for the 2nd reaction. Li compound was added during the reaction. After a 10 h retention, the gel of hectorite was formed. The XRD pattern of the synthesized one was coincided with that of natural hectorite and SEM study revealed uniform grains 50 m in diameter. The d001 basal spacing of the product moved from 12 to $17.4\;{\AA}$ after glycolation treatment. The measured value of CEC and the swelling capacity was 90 cmol/kg and $60{\sim}70\;mL/2\;g$, respectively.

Characteristics of Hydrodynamic Dispersion Using a Natural Gradient Tracer Test in a Fractured Rock at the Jwacheon-dong, Busan City (부산시 좌천동 단열암반층에서 자연구배 추적자시험을 이용한 수리분산특성 연구)

  • Chung Sang-Yong;Kang Dong-Hwan;Kim Byung-Woo
    • The Journal of Engineering Geology
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    • v.16 no.3 s.49
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    • pp.245-254
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    • 2006
  • Using a natural gradient tracer test, the characteristics of hydrodynamic dispersion according to each depth of a fractured rock were studied, and the effective porosity and longitudinal dispersivity of the fractured rock were estimated. The difference of vertical hydrodynamic dispersion was identified by concentration breakthrough curves linear regression analyses of bromide concentrations according to depths versus time, and hydraulic fracture characteristics at two intervals of the monitoring well. Higher concentration and faster arrival time at GL- 18 m depth (RQD 13%, average joint spacing 2 cm, TCR 100%) than at GL- 25 m depth (RQD 41%, average joint spacing 7 cm, TCR 100%) resulted from shorter distance and more fractures. Tracer was transported through the 1 st fractures until the arrival of its peak concentration and through the 2nd fractures or matrix diffusion after the arrival of its peak concentration. The increase/decrease slopes of bromide concentration versus time were 3.46/-1.57 at GL-18 m depth and 3.l9/-0.47 at GL- 25 m depth of the monitoring well. So the faster bromide transport was confirmed at GL- 18 m depth with more fractures. The concentration increment of bromide was fitted by a Gaussian function and the concentration decrement of bromide was fitted by an exponential function. Effective porosity and longitudinal dispersivity estimated by CATTI code were 10.50% and 0.85 m, respectively.

Regional snows scenario for the support systems Analysis (지역별 제설 시나리오 응원체계 구축연구)

  • Kim, Heejae;Oak, Youngsuk;Kim, Geunyoung
    • Journal of the Society of Disaster Information
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    • v.13 no.2
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    • pp.163-172
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    • 2017
  • Because of abnormal weather, a heavy snow on the Northern latitudes occurs frequently. This has resulted in significant damage and recovery costs. In korea, it has been declared a special disaster area due to heavy snowfall in Gangneung and Pohang 2004, 2005 and 2011, so there was a revision of action instruction for the road snow removal. Although, in our current system, snow removing methodology, regional equipment holdings, and snow responsible interval, respectively, has been classified by the National Highway, near cities and provinces support system not yet prepared. Only, if snow removing is not possible within the region itself, which contained the contents of "support and assistance to military or nearby offices requests". In this thesis, we studied the disaster scenario development according to heavy snow and the response and support system to the features of each regional. For the scenario deduction, we preferentially collected day snowfall and disaster yearbook data to regionals, classified similar pattern and plotted GIS snow map. We also classified heavy snow disaster by region and type and we deduced five-step scenario. The five-step scenario is nationwide(1st-stage), the National Capital region(2nd-stage), the Chungcheong Provinces(3rd-stage), the Kangwon province(4th-stage) and the Ch?l a provinces(5th-stage). Therefore we build near provinces support system according to five-step scenario.

Effect of Various Levels of Perilla Seed Powder on the Fermentation of Puchukimchi (부추김치의 발효숙성에 들깨가루 첨가량이 미치는 영향)

  • 장명숙;박문옥
    • Korean journal of food and cookery science
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    • v.14 no.3
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    • pp.232-240
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    • 1998
  • The effect of various levels of perilla seed powder on physicochemical, microbiological, and sensory characteristics of Puchukimchi during fermentation was investigated. Puchukimchi is a kind of korean kimchi made with Leek (Allium odorum L.). In a laboratory model of Puchukimchi made with various levels (0, 1, 2, 3, 5%) of perilla seed powder fermentated at 10$^{\circ}C$ up to 45 days. As a fermentation progressed, the initial high decrease in pH has been retarded in all the treatments, of which the delaying extent was noted from Puchukimchi made with perilla seed powder. The pH dropped with accumulation of total acidity. The total vitamin C content increased sharply at the palatable period of Puchukimchi during initial fermentation and then, especially from the 8th-day, gradually decreased later following sigmoidal changing pattern. And the total vitamin C content was the highest in the Puchukimchi made with 5% perilla seed powder. The lactic acid bacteria count increased remarkably and then decreased gradually after optimum ripening period, i.e., the palatable period of Puchukimchi during fermentation. During the early stage of fermentation, sensory evaluation showed the higher scores on the overall taste of Puchukimchi made with 3, 5% on the 2nd-day, 1, 2% on the 5th-day of fermentation. However, the trend of acceptability has been reversed by the Puchukimchi made with little amount of perilla seed powder, more notably 2% at the later stage of fermentation.

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The Effect of Interleukin $1-{\beta}$, Platelet Derived Growth Factor-BB and Transforming Growth $Factor-{\beta}$ on the expression of PDLs17 mRNA in the Cultured Human Periodontal Ligament Fibroblasts (($IL-1{\beta}$), PDGF-BB 그리고 $TGF-{\beta}$가 사람 배양 치주인대 섬유모세포의 PDLs17 mRNA의 발현에 미치는 영향)

  • Lirn, Ki-Jung;Han, Kyung-Yoon;Kirn, Byung-Ock;Yeorn, Chang-Yeob;Park, Joo-Cheol
    • Journal of Periodontal and Implant Science
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    • v.31 no.4
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    • pp.787-801
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    • 2001
  • The molecular mechanisms control the function of PDL(periodonta1 ligament) cells and/or fibroblasts remain unclear. PDLsl7, PDL-specific gene, had previousely identified the cDNA for a novel protein from cultured PDL fibroblasts using subtraction hybridization between gingival fibroblasts and PDL fibroblasts. The purpose of this study was to determine the regulation by growth factors and cytokines on PDLsl7 gene expression in cultured human periodontal ligament cells and observe the immunohistochemical localization of PDLsl7 protein in various tissues of mouse. Primary PDL fibroblasts isolated by scraping the root of the extracted human mandibular third molars. The cells were incubated with various concentration of human recombinant $IL-1{\beta}$, PDGF-BB and TGF\;${\beta}$ for 48h nd 2 weeks. At each time point total RNA was extracted and the levels of transcription ere assessed by reverse transcription-polymerase chain reaction (RT-PCR assay). polyclonal antiserum raised against PDLsl7 peptides, CLSVSYNRSYQINE and SEAVHETDLHDGC, were made, and stained the tooth, periodontium, developing bone, bone marrow and mid-palatal suture of the mouse. The results were as follows. 1. PDLsl7 mRNA levels were increased in response to PDGF (10ng/ml) and $TGF\;{\beta}$(20ng/ml) after treatment of the $IL-1{\beta}$, PDGF-BB and $TGF{\beta}$for 48 h. 2. PDLsl7 was up-regulated only by $TGF{\beta}$(20 ng/ml) after treatment of the $IL-1{\beta}$, PDGF-BB and $TGF\;{\beta}$ for 2 weeks and unchanged by the other stimulants. 3. PDLsl7 was a novel protein coding the 142 amino acid peptides in the ORF and the nucleotide sequences of the obtained cDNA from RT-PCR was exactly same as the nucleotides of the database. 4. Immunohistochemical analysis showed that PDLsl7 is preferentially expressed in the PDL, differentiating osteoblast-like cells and stromal cells of the bone marrow in the adult mouse. 5. The expression of PDLsl7 protein was barely detectable in gingival fibroblasts, hematopoetic cells of the bone marrow and mature osteocytes of the alveolar bone. These results suggest that PDLsl7 might upregulated by PDGF-BB or $TGF{\beta}$ and acts at the initial stage of differentiation when the undifferentiated mesenchymal cells in the bone marrow and PDL differentiate into multiple cell types. However, more research needs to be performed to gain a better understanding of the exact function of PDLsl7 during the differentiation of bone marrow mesenchymal and PDL cells.

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Studies on the Seed Production of the Puffer Takifugu rubripes (Temminck et Schlegel) (자주복, Takifugu rubripes (Temminck et Schlegel)의 종묘양산에 관한 연구)

  • RHO Sum;JUNG Yun-Seok
    • Journal of Aquaculture
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    • v.6 no.4
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    • pp.295-310
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    • 1993
  • As a part for the development of effective mass production techniqne of the puffer, Takifugu rubripes. seedfish, an investigation was carried out in 1989. 1991 and 1992 to determine the optimum starting feeding time of rotifers as a starting food for the fish larvae, and to find out the influences of the light intensitiy and LD cycles on the Artemia consumption by the larvae. The optimum starting time of feeding with rotifer. Brachionus Plicatilis was 2nd to 5th day from hatching resulting in survival of $96.5\~90.0\%$. Optimum light conditions for maximum feeding for fish larvae with Artemia were 1000 lux in 6mm, 600 lux in 8mm, and 200 lux in total length of 10-12 mm larvae. Relationship between total length (X: mm) of fish (6-12 mm in total length) and light intensity (Y: lux) for maximum feeding was:Y=2200-200X (r=-1.000). Relationship between days from hatching (X) and total length (Y: mm) of puffer fry was :Y=1.6427+0.2540X(r=0.9814) for 3 to 36 days after hatching, Y=-33.1452+1.1867X(r= 0.9854) for 36 to 68 days after hatching. Survival rate for 68 days after hatching was $24.1\%$ and the range of water temperature during this period was $21.25\pm1.67^{\circ}C$.

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A CLINICAL AND RADIOGRAPHIC STUDY OF DENTIGEROUS CYSTS (함치성 낭의 임상 방사선학적 연구)

  • Lee Kang-Sook;Choi Karp-Shik
    • Journal of Korean Academy of Oral and Maxillofacial Radiology
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    • v.25 no.2
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    • pp.399-408
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    • 1995
  • The purpose of this study was to obtain information on the clinical and radiographic features of the dentigerous cysts in the jaws. For this study, the authors examined and analysed the clinical records and radiographs of 233 patients who had lesions of dentigerous cyst diagnosed by clinical and radiographic or histopathological examinations. And the obtained results were as follows: 1. Dentigerous cysts occurred the most frequently in the 2nd decade(38.2%) and occurred more frequently in males(67.4%) than in females(32.6%). 2. The most common clinical symptom was swelling of the jaw(33.9%), and the lesions were treated by the method of surgical removal. 3. The type of lesions was mainly observed as central type(72.5%), and size of the lesion was most frequently observed 2 - 2.9cm in the widest length. 4. The lesions were most frequently observed well-defined outline with hyperostotic border(49.8%), and smooth margin(73.4%), and homogeneous lesional radiolucency(79.4%). 5. Cortical thinning and expansion of the lesions(82.0%) were observed, and their direction were most frequently observed toward buccal side(64.0%). 6. The effect on the causative tooth were observed as tooth displacement(41.2%) and delayed root development(l9.3%), and the distance between cemento-enamel junction and lesional wall attachment of the causative tooth was mainly observed as below 2mm(79.6%). 7. The effect on the adjacent tooth were observed as loss of lamina dura(66.8%), root resorption(33.9%), and tooth displacement(31.5%). 8. The effects on the adjacent anatomic structures were observed as displacement of the mandibular canal(46.5%) and maxillary sinus or nasal cavity(72.2%).

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Effect of Planting Distance and Seeding Date on the Tiller Occurrence, Growth Characteristics of Sorghum (Sorghum bicolor L.) Resources (재식거리 및 파종기 이동에 따른 수수자원의 분얼발생 및 생장 특성)

  • Han, Tae-Kyu;Yoon, Seong-Tak
    • Korean Journal of Plant Resources
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    • v.30 no.4
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    • pp.427-434
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    • 2017
  • This experiment was conducted to investigate the aspect of tiller occurrence, growth and yield of sorghum according to planting distance and sowing date. The object of this experiment is to get basic data to inhibit non-productive tillers uneconomical and cumbersome for mechanical harvesting. Also another object was to evaluate optimum planting distance and sowing date in central district area. Total number of tillers was more in 80 cm ridge than 60 cm ridge and it was increased as the planting distance was wider from 15 cm to 30 cm on the each ridge. Ratio of effective tillers was higher in 60 cm ridge than 80 cm ridge and it was decreased as planting distance was wider from 15 cm to 30 cm. The lower the planting distance, the more increased total number of tillers, whereas effective tillers was decreased as planting distance was high. Average of total number of tillers of three varieties was higher in sowing date of May 2 (1st sowing date), whereas ratio of effective tillers was the highest in sowing date of May 23 (2nd sowing date). Hwanggeumchal showed the highest total number of tillers (1.2 tillers), while Moktaksusu had the lowest total number of tillers (0.8 tillers) among three varieties. There were no significant difference between planting distance and days to heading and ripening date from seeding. Culm length increased as planting distance was high, but ear length, grains per ear and 1000 grain weight were decreased on the other hand. The highest yield of sorghum per 10a was obtained from $60{\times}20cm$ planting distance among 6 planting distances.