• Title/Summary/Keyword: NAL1

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Optimization of Cyclodextrin Glucanotransferase Immobilization on Amberlite IRA-900 (Amberlite IRA-900을 이용한 cyclodextrin glucotransferase의 최적 고정화)

  • Seo, Hyo-Jin;Jung, Il-Hyong;Nam, Soo-Wan;Kim, Byung-Woo;Kim, Sung-Koo
    • Journal of Life Science
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    • v.14 no.5
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    • pp.794-799
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    • 2004
  • Cyclodextrin glucanotransferase (CGTase) produced by Bacillus subtilis NAl/pKBl was used for the production of cyclodextrin (CD). The enzyme was purified by ion exchange and gel filtration chromatography. The purified enzyme exhibited its maximum activity in the pH range of 6.0 to 7.0 and temperature range of 60 to $70^{\circ}C$. Immobilization of purified CGTase was carried out with various immobilization matrices. Amberlite IRA-900, a strong basic anion exchange resin, showed the highest immobilization ability (38 units per gram resin). Optimal pH and temperature for enzymatic reaction of the immobilized CGTase were pH 6.0 and 60t. The activity of immobilized CGTase maintained more than a month and could be reused for a month in a continuous enzyme reactor for the production of CD.

Preparation of Antibiotic-Resistant Bradyrhizobium japonicum and Its Inoculation Effects on Soybean [Glycin max(L.) Merr] (항생물질(抗生物質) 표식(標識) 근류균(根瘤菌)의 조제(造製)와 그 접종효과)

  • Kim, Kwang-Sik;Kim, Kil-Yong;Son, Bo-Gyun;Lee, Young-Hwan;Kim, Yong-Woong;Seong, Ki-Young
    • Korean Journal of Soil Science and Fertilizer
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    • v.25 no.2
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    • pp.181-188
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    • 1992
  • This study was carried out to evaluate the fate of inoculant Bradyrhizobium japonicum and the inoculation effect on soybean in complex soil environment. To moniter Rhizobium strains from the root, streptomycine and streptomycine and nalidixic acid resistant marker strains were prepared by spontaneous mutagenesis. The characteristics and properties of antibiotic marked strains were not altered by the mutagenesis. The comparison of properties of wild type and antibiotic resistant Bradyrhizobium strains are summarized as follow : 1) The strains of USDA110K-$STR^r$, USDA110N-$STR^r$ and R318-$STR^r$ showed weak tolerance to pH 9.0. The utilization of carbon sources by fast growing group was different from that of slow growing group. The marked strains of R214-$STR^rNAL^r$, USDA110K-$STR^r$ and USDA110N-$STR^r$ was doubtful in utilization of sorbitol and R138-$STR^rNAL^r$ was doubtful in utilization of xylose as a carbon source. 2) By examining the agglutination reaction of serogroups, the strains used were identified as different ones. There were no differences between wild type and marked strains in agglutination titer values. 3) The plasmid size of fast group was slightly greater than that of slow group. However, there was no differences in plasmid size between the wild type and antibiotic resistant strains. This result indicates that the antibiotic resistance was not encoded in plasmid. 4) The recovery of the inoculated strains was up to 12.5 % in soybean cultivated soil and was up to 25 % in soybean uncultivated soil. 5) When the wild type or marked strains were inoculated. there was no significant effect on soybean plant, whereas the inoculation effect was pronounced in soybean uncultivated soil. The inoculation effect seemed to be more pronounced in wild type strains than antibiotic resistant strains. however, the difference was not significant.

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Pseudomonas sp. strain DJ77 균주에서 extradiol dioxygenase 를 암호화하고 있는 phnE 유전자의 염기배열

  • 김영창;신명수;윤길상;박영순;김욱현
    • Korean Journal of Microbiology
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    • v.30 no.1
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    • pp.8-14
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    • 1992
  • Nucleotide Sequence of phnE Gene Encoding Extradiol Dioxygenase fromPseudomonas sp. Strain DJ77Kim, Young-Chang'.", Myeong-Su Shin1, Kil-Sang Younl, Young-Soon Park1, andUg-Hyeon Kim'.' (Department of Microbiology, C'hungbuk National University.Cheongju 360-763, KOREA. and 'Research Center for Molecular Microbiology,Seoul National University)nal University)

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A study on the fabrication of PI thin films by VDP method (증착중합법에 의한 폴리이미드 박막의 작성에 관한 연구)

  • Kim, H.G.;Han, S.O.;Kim, J.S.;Park, K.H.;Jin, K.S.;Lee, D.C.
    • Proceedings of the KIEE Conference
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    • 1994.07b
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    • pp.1394-1396
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    • 1994
  • Polyimide thin films were fabricated an using vapor deposition polymerization apportus, and their FT-IR and TGA characteristics were investigated. The peaks of $720cm^{-1}$ and $1380cm^{-1}$ show C=O stretch mode and C-N stretch mode, and that of the cured polyimide at $350^{\circ}C$ were sturated. $T_d$(Depolymerization temperature) was showed at $405^{\circ}C$ from research of thermal resistivity characteristics by TGA It was possible to fabrication of polyimide thin film by VDPM.

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Molecular Dynamics Simulation Studies of Zeolite A. VIII. Structure and Dynamics of Na+ ions in a Non-Rigid Dehydrated Zeolite-A Framework

  • 이송희;최상구
    • Bulletin of the Korean Chemical Society
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    • v.20 no.5
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    • pp.587-591
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    • 1999
  • A molecular dynamics simulation study on the structure and dynamics of Na+ ions in non-rigid dehydrated Na12-A zeolite framework at 298.15 K was conducted using the same method reported in previous studies on rigid and non-rigid Na12-A zeolite frameworks. The agreement between the experimental and calculated results for the zeolite-A framework atoms of structural parameters for non-rigid dehydrated Na12-A zeolite is generally quite good, and for the adsorbed Na+ions the agreement is acceptable. The calculated bond lengths are generally in good agreement with the experimental results and other theoretical data. The calculated IR spectrum by Fourier transform of the total dipole moment autocorrelation function shows two major peaks around 2700 cm-1 and 7000 cm-1. The former appeared in the calculated IR spectra of non-rigid zeolite-A framework only system and the latter remains unexplained except, perhaps, indicating a new formation of a vibrational mode of the framework due to the adsorption of Na+ ions. The peaks above 6200-6800 cm-1 in non-rigid dehydrated Nal2-A zeolite are much larger than those in non-rigid dehydrated H12-A zeolite.

Assessment of natural radioactivity in soil and olive mill pomace utilizing nal (TI) gamma-ray spectrometry and low background alpha/beta counting system

  • Amani Kraishan;Mohammad Abu Shayeb;Hafedh Belmabrouk;Ahmad Ali Husein Qwasmeh;Muzahir Ali Baloch
    • Nuclear Engineering and Technology
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    • v.56 no.5
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    • pp.1925-1931
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    • 2024
  • The study conducted in the northwest region of Jordan aimed to assess the levels of natural radioactivity in soil and olive mill pomace (OMP) samples. The researchers used Nal (TI) gamma-ray spectrometry to measure the activity concentrations of 226Ra, 232Th, 40K, and 137Cs in the samples. The average activity concentrations of 226Ra, 232Th, 40K, and 137Cs in the soil samples were found to be 18.624 ± 5.82, 12.276 ± 5.728, 518.33 ± 212.57, and 0.140 ± 0.09 (Bq, kg-1), respectively. In the OMP samples, the average activity concentrations of 226Ra, 232Th, and 40K were 7.272 ± 4.386, 3.454 ± 1.503, and 169.997 ± 81.873 (Bq kg-1), respectively, and no 137Cs was detected. The study also investigated fundamental parameters associated with radon, specifically the radon emanation coefficient (RnEC) and radon mass exhalation rate (Ex). The RnEC values ranged from 0.621 to 0.78 (Bq kg-1), with an average value of 0.71 ± 0.06 (Bq kg-1). The estimated Ex from the soil samples ranged from 65.83 to 124.86 (mBq kg-1h-1), with an average value of 99.74 ± 21.73 (mBq kg-1h-1). Regarding radiological hazards, the study examined various parameters, including radium equivalent activity, external and internal hazard indices, gamma and alpha indices, absorbed gamma dose rate, and excess lifetime cancer risk. All of these assessed values were found to be below the worldwide recommended limits for radiological safety. Additionally, the study analyzed the concentrations of gross alpha and gross beta radioactivities in soil and OMP samples. The soil samples had an average gross alpha activity of 4.642 ± 1.04 (Bq kg-1) and an average gross beta activity of 48.13 ± 14.50 (Bq kg-1). The OMP samples showed an average gross alpha activity of 0.32 ± 0.27 (Bq kg-1) and an average gross beta activity of 59.19 ± 12.94 (Bq kg-1). Overall, the obtained results are crucial for evaluating the radiological risks associated with natural radioactivity in the northwest region of Jordan. The findings establish baseline data for comparison and reference for radioactivity levels in the environment.

Thermal Neutron Activation Analysis of Vanadium and Manganese in Ginseng using 3.76-Minute Vanadium-52 and 2.58 Hour Manganese-56 (人蔘中의 Vanadium 및 Manganese의 熱中性子에 依한 放射化分析)

  • Chong Jin Lee;Chong Kuk Kim;Jin Ha Park
    • Journal of the Korean Chemical Society
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    • v.7 no.1
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    • pp.13-16
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    • 1963
  • Thermal neutron activation analysis was applied to determine the trace amount of Vanadium and Manganese in Buyo and Kumsan Ginseng. These elements have been regarded to have great nutritional value and one of the indispensable factor in the growth of ginseng. The TRIGA MARK II Reactor in Atomic Energy Research Institute was used for the neutron source. The samples were irradiated for 10 minutes for Vanadium and for 5 minutes for Manganese at the neutron flux of about $1.28{\times}10^{12}n/cm^2/sec$ and the RCL 256 Channel Pulse-Height Analyzer connected with $2"{\times}2"$ Nal(Tl) was used for activity determination. The amounts were about 0.02 ppm for Vanadium and 20 ppm for Manganese, and it was also found that the amounts of the elements were slightly different depending on the kinds of ginsengs.

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Effect of SAENGMAEGSAN extract on the Cardiovascular System and Regional Cerebral Blood Flow (생맥산(生脈散)이 심혈관계(心血管系) 및 국소뇌혈류량(局所腦血流量)에 미치는 영향(影響))

  • Shin, Dae-Chul;Kim, Young-Kyun
    • The Journal of Internal Korean Medicine
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    • v.20 no.1
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    • pp.153-166
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    • 1999
  • SAENGMAEGSAN(SMS) has been used in oriental medicine for many years as a therapeutic agent for cerebral disease. The effect of SMS on the vascular system is not known. The purpose of this study was to determine the effect of SMS on blood pressure, regio-nal cerebral blood flow(rCBF). 1. Blood pressure did not change by SMS in rats. 2. rCBF was increased by SMS in a dose-dependent manner. 3. Pretreatment with propranolol. methylene blue and indomethacin significantly inhibited SMS induced increase in rCBF. These results suggest that SMS causes a diverse response of blood pressure, regional cerebral blood flow (rCBF) and pial arterial diameter. The increase in rCBF is also mediated by prostaglandins. cyclic GMP and adrenergic ${\beta}$receptor.

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A new record of Scutellaria (Lamiaceae) in Korean flora: S. guilielmii A. Gray

  • KIM, Jung-Hyun;YANG, Young-Tae;LIM, Chae Eun;KIM, Jin-Seok
    • Korean Journal of Plant Taxonomy
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    • v.51 no.1
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    • pp.103-108
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    • 2021
  • A new distribution of Scutellaria guilielmii A. Gray is discovered in Korea. This species was collected from seashores on Pyoseon-ri, Pyoseon-myeon, Seogwipo-si, Jeju-do. S. guilielmii is distinguished from other related Korean taxa of the genus by having broadly winged nutlets (fruits). Here, we provide a precise description, illustrations, key to the related taxa, and photographs of its habitat. The new Korean name is given as 'Nal-gae-gol-mu-kkot', considering the broadly winged nutlets (fruits). In addition, new habitats are likely to be discovered through plant biodiversity surveys of the southwestern coastal islands.

Field tests of the radiation detectors for environmental radiation monitoring around KORI nuclear power plants (고리원자력 주변 환경방사선 감시를 위한 방사선 측정기의 현장 성능 시험)

  • 최성수;신대용;조규성;하달규
    • 제어로봇시스템학회:학술대회논문집
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    • 1997.10a
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    • pp.1371-1374
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    • 1997
  • We had developed the on-line environmental monitoring system which has installed around Kori Nuclear Power Plants and will be taken the place of the existing system. The system consists of a main computer and 11 sets of radiation monitoring post equipments. Nal(Tl) scintillation detectro was adopted in addition to ion-chamber detector and implemented with DCU(Dose Conversion Unit) and SCA(Single Channel Analyzer). Compared with the existing system, it has revised feature in the radiation measurements which are detection of artificial radioactivity and 2-ways of the radiatiion detectors. The field test trsults show that the developed radiation detecting equipments can measure environmental radiation withn 5.0% of the theoretical value.

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