• Journal of Plant Biotechnology
• /
• 제5권2호
• /
• pp.107-113
• /
• 2003

The effect of sodium sulphate on shoot induction and multiple shoot formation from nodal explants of Vitex negundo L. was tested on Murashige and Skoog's (MS) medium fortified with different auxins, cytokinins and sucrose. Highest percentage $(97.78\%)$ of explants for shoot induction and multiple shoot (20.68/explant) production were observed in the combination treatment of $N^6-Benzyl$ adenine (BA) $(17.80\;{\mu}M/L)$, ${\alpha}-Naphthalene$ acetic acid (NAA) $(2.15\;{\mu}M/L)$ and $5\%$ sucrose supplemented with 100 mg/L sodium sulphate. In vitro raised shoots were rooted on the half-strength MS medium fortified with different concentrations of NAA, Indole-3-acetic acid (IAA), and Indole-3-butyric acid (IBA) alone and in combinations. Among the treatments, $4.90\;{\mu}M/L$ of IBA was found most effective $(95.56\%)$ in inducing roots. The rooted plantlets were shifted to glasshouse for acclimatization and later transferred to the field with cent percent survival. Furthermore, in vitro flowering was observed in the shoots cultured on MS medium supplemented with BA $(8.90\;{mu}M/L)$ and NAA $(1.61\;{\mu}M/L)$.

• 한국자원식물학회지
• /
• 제24권3호
• /
• pp.280-285
• /
• 2011

In this study, we established a novel somatic embryogenesis and plant regeneration system through cell suspension culture of white dandelion (Taraxacum coreanum NAKAI.). Embryogenic calli could be initiated from leaf and root explants of sterile seedlings on solid Murashige and Skoog (MS) medium supplemented with 1.0 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) after 3-week cultures. To proliferate embryogenic calli rapidly, cell suspension culture was performed with transferred to liquid MS medium with various combinations of plant growth regulators (PGRs) including 2,4-D, ${\alpha}$-naphthalene acetic acid (NAA), indole-3-acetic acid (IAA), $N^6$-benzylamino purine (BAP), thidiazuron (TDZ), and kinetin. During suspension cultures, embryogenic calli not only greatly proliferated, but shoot organogenesis also simultaneously occurred from the surface of somatic embryos. Among them, TDZ at lower concentration, 0.1 mg/L produced the highest efficiency of somatic embryo formation and shoot organogenesis. Rooting of embryogenic calli with adventitious shoots was done on solid MS medium containing 0.1 mg/L NAA and 0.3% activated carbon. Nearly 80% of embryogenic calli with shoot organogenesis could be rooted normal. Well-rooted plantlets were transferred into pots under a greenhouse condition, and plants derived from this system appeared phenotypically normal.

• Journal of Plant Biotechnology
• /
• 제6권3호
• /
• pp.189-192
• /
• 2004

Adventitious multiple shoots were developed from leaf, petiole and internode explants of Geophila reniformis D. Don. on MS medium supplemented with various concentrations of $N^6$-benzylaminopurine (BAP) or Kinetin (KIN) alone or in combination with indole-3-acetic acid (IAA). Leaf showed maximum organogenetic potential, followed by petiole and internode. Murashige and Skoog (MS) medium supplemented with 22.22 $\mu{M}$ BAP and 4.57 $\mu{M}$ IAA induced maximum shoot buds from leaf explants. Internodal segments showed low potential of direct organogenesis. The regenerated shoots rooted the best in presence of 10.75 - 13.44 $\mu{M}$ $\alpha$-naphthalene acetic acid (NAA) along with 2.22 $\mu{M}$ BAP, and were successfully established in the field with a survival rate of 89.11%.

• Journal of Plant Biotechnology
• /
• 제2권1호
• /
• pp.51-54
• /
• 2000

Leaf explants of the cucumber (Cucumis sativus L.) were cultured on Murashige and Skoog's (MS) medium supplemented with various concentrations of $\alpha$-naphthalene acetic acid (NAA) and 6-benzylaminopurine (BAP). Direct shoot orgnogenesis as well as callus formation with somatic embryos and multiple shoots was observed from leaf explants of cvs. Shinhukjinju and Chungjang. The highest frequency of shoot formation 80% was observed on MS medium supplemented with NAA/BAP (5.0 ${\mu}{\textrm}{m}$/2.5 ${\mu}{\textrm}{m}$), with explants forming 3-7 shoots. Shoots formation occured within 3 to 4 weeks. Only one subculture of calli was required for plant regeneration on normal growth regulator-free medium. Plantlets transferred to soil developed into plants of normal appearance, which flowered and set fruits.

• Plant Resources
• /
• 제8권3호
• /
• pp.286-292
• /
• 2005

Shoot induction system was developed in the recalcitrant plant species, Brassica rapa ssp. rapifera by using optimum selection of profit organ, phytohormone combination, seedling age and kind of culture container. Out of in vitro cultured leaf segment, petiole, hypocotyl, and cotyledon with petiole, only cotyledon with petiole derived from 4 day-old seedlings induced multiple shoot. The optimum combination of auxin and cytokinin for the multiple shoot induction was MS medium containing 5mg/L BA and 0.5mg/L NAA. The major factors for multiple shoot propagation were part of plant organ, age of seedling, and ratio of auxin and cytokinin. In addition, shoot regeneration was promoted in the 100ml Erlenmeyer flask compared with the $90mm{\times}20mm$ Petri-dish. The induced shoots formed roots easy on MS medium containing 0.1mg/L IBA and the whole plants were successfully cultivated in soil.

• 원예과학기술지
• /
• 제35권2호
• /
• pp.252-264
• /
• 2017

Tea is one of the most consumed beverages worldwide and the relatively high levels polyphenols is benefit for health. In this study, we developed an efficient system for proliferation of callus from 'Anji Baicha', a cultivar of tea (Camellia sinensis). Callus tissue was initially induced by culturing leaf explants on medium containing different plant growth regulators. For callus induction, thidiazuron (TDZ) was more effective than 2,4-dichlorophenoxyacetic acid (2,4-D), ${\alpha}-naphthalene$ acetic acid (NAA), and $N^6-benzyladenine$ (BA). The frequency of callus induction from leaf explants reached 90.21% on $1.0mg{\cdot}L^{-1}$ TDZ and the developed callus was reddish and friable. We also tested the effect of different concentrations of NAA, 2,4-D, indole 3-acetic acid (IAA), BA, and TDZ, alone and in combinations, on callus proliferation. Medium supplemented with TDZ in combination with IAA was suitable for callus proliferation and accumulation of tea polyphenols. The growth index value and tea polyphenol content of callus cultured on MS medium containing $0.5mg{\cdot}L^{-1}$ TDZ and $1.0mg{\cdot}L^{-1}$ IAA was maximally 1,351% and 23.24%, respectively, and the relative abundance of epicatechin was as high as 17.449%. We also measured the antioxidant activity of all samples and the callus with the highest tea polyphenol content also exhibited high potential radical scavenging activity.

• 한국식품과학회지
• /
• 제14권2호
• /
• pp.179-183
• /
• 1982

Stevia엽(葉) 조직(組織)에서 callus의 유기(誘起) 및 배양(培養)을 위(爲)한 조건(條件)을 조사(調査)하고 이들 배양(培養) callus가 감미성분(甘味成分)인 stevioside를 생성(生成)하는지를 조사(調査)하여 다음과 같은 결과(結果)를 얻었다. Callus유기(誘起)에는 Linsmaier-Skoog기본배지(基本培地)에 benzyl-adenine $10^{-6}M$과 ${\alpha}-naphthalene$ acetic acid $10^{-5}M$을 함유(含有)한 배지(培地)가 가장 효과적(效果的)이였으며 callus의 성장률(成長率)도 비교적 양호(良好)하였으며 이들 callus는 배양후(培養後) 15일(日)부터 20일(日) 사이에 가장 빠른 성장률(成長率)을 나타내었다. Stevia callus가 생성(生成)한 stevioside는 TLC에 의(依)해 동정(同定)하였으며, 이는 정제(精製)한 표준품(標準品)의 stevioside와 동일(同一)한 Rf값인 0.50을 나타내었다. 또한 stevioside을TLC-FID analyzer에 의(依)해 분석(分析)한 결과(結果), 배양(培養) callus 건물중(乾物重) 100g당(當) 260mg이였다.

• 환경생물
• /
• 제19권4호
• /
• pp.248-253
• /
• 2001

붉나무에서 면충에 의하여 형성되는 오배자 및 꽃오배자면충의 생장과정과 시기별로 축적되는 tannin 함량을 분석하고 동시에 이들 충영으로부터 유래된 배양 callus에 IAA등을 처리하여 생육 및 tannin 축적과의 관계를 조사하여 다음과 같은 결론을 얻었다. 충영의 발달은 오배자가 꽃오배자 보다 이른 시기인 6월초에 시작되었고 완숙단계도 오배자가 9월초까지 지속됨으로서 오배자가 면충은 꽃오배자 보다 면충 보다 생활사가 길며 먹이 활동 또한 활발한 것으로 나타났다. 충영의 tannin 함량은 오배자의 경우 초기생육기부터 전 생육기간에 거쳐 60${\sim]$70%의 높은 함량을 나타냄으로서 tannin의 주된 원료로서의 가치를 확인할 수 있었다. 반면 꽃오배자는 건물당 최고 10% 정도에도 미치지 못하는 낮은 함량으로 이것은 단지 면충의 서식지로서의 역할만이 인식되었다. 충영 유래 배양 callus에서 IAA 10$^{-5}$mole 농도는 생육증가에 가장 효과적이었으나, IAA처리에 의한 tannin축적현상은 10$^{-5}$mole등 농도별 시험구에도 유발되지 않았다. 또한 대조처리한 NAA시험구에서도 tannin축적은 확인되지 않았다. 결국 붉나무 오배자 충영의 tennin 축적형상은 기주식물과 면충의 생활사가 연계된 특이관계에서만 가능한 것으로 사료된었다.

• 한국약용작물학회지
• /
• 제20권6호
• /
• pp.479-486
• /
• 2012

The production of adventitious roots derived from root explant of Echinacea angustifolia and its secondary metabolite content were assessed in different types and levels of auxin. The induction of adventitious roots from root explant cultured in Murashige and Skoog solid medium supplemented with 1.0 mg/L indole -3-butyric acid (IBA) attained highest as 20.87 mg fresh weight and 3.07 mg dry weight per culture but root suspension culture at the same concentration of IBA enhanced biomass production as 3.07 g fresh weight and 0.38 g per culture after 4 weeks in culture. 3.0 mg/L ${\alpha}$-naphthalene acetic acid (NAA) treatment had similar effect on root biomass production as 3.07 g fresh weight and 0.38 g per culture with liquid suspension culture, whereas adventitious roots exposed to over 3.0-5.0 mg/L IBA or 5.0 mg/L NAA were less responsive by reducing the number of adventitious roots and/or changing root morphology such as short and thick. The content of secondary metabolites such as phenolic, flavonoids and total caffeic acid in adventitious roots cultured on MS medium supplemented with 1.0 mg/L IBA were attained highest as 27.20, 9.60. 10.67 mg/g dry weight, respectively. Overall, the best production of root biomass and secondary metabolites were given by 1.0 mg/L IBA.

• KSBB Journal
• /
• 제24권1호
• /
• pp.59-64
• /
• 2009

The callus formation from inferior leaf of Aloe saponaria was induced in M & S medium supplemented with 10-30 ${\mu}M$ NAA (${\alpha}$-naphthalene acetic acid) and 3-7 ${\mu}M$ kinetin under incubation in the dark at $25^{\circ}C$ for 6 weeks. The hot water extract ($100^{\circ}C$, 24 hrs) from cultured callus was obtained and the components analysis for the extract were examined to determine the callus can synthesized the bioactive component such as Aloe polysaccharide. The freeze dried extract contained the sugar of 53.2%, protein of 7.3%, ash of 18.5% and water of 21% (w/w). Two fractions (Fr-I and Fr-II) were obtained by Sepharose CL-4B gel permeation chromatography and Fr-I, major fraction was further purified with dialysis. From sugar analysis by TLC and GC, the purified Fr-I fraction consisted of glucose (77.6%), galactose (17.7%), mannose (4.7%, w/w) and uronic acid (trace). The molecular weight of purified Fr-I fraction determined by GPC was about 110 kDa.