Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
권호 표지
DOI QR코드

DOI QR Code

Establishment of Cell Suspension Cultures and Plant Regeneration in White Dandelion (Taraxacum coreanum NAKAI.)

  • Sun, Yan-Lin (Department of Bio-Health Technology, Kangwon National University) ;
  • Kim, Jae-Hak (Department of Bio-Health Technology, Kangwon National University) ;
  • Hong, Soon-Kwan (Department of Bio-Health Technology, Kangwon National University)
  • Received : 2010.11.11
  • Accepted : 2011.05.31
  • Published : 2011.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

In this study, we established a novel somatic embryogenesis and plant regeneration system through cell suspension culture of white dandelion (Taraxacum coreanum NAKAI.). Embryogenic calli could be initiated from leaf and root explants of sterile seedlings on solid Murashige and Skoog (MS) medium supplemented with 1.0 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) after 3-week cultures. To proliferate embryogenic calli rapidly, cell suspension culture was performed with transferred to liquid MS medium with various combinations of plant growth regulators (PGRs) including 2,4-D, ${\alpha}$-naphthalene acetic acid (NAA), indole-3-acetic acid (IAA), $N^6$-benzylamino purine (BAP), thidiazuron (TDZ), and kinetin. During suspension cultures, embryogenic calli not only greatly proliferated, but shoot organogenesis also simultaneously occurred from the surface of somatic embryos. Among them, TDZ at lower concentration, 0.1 mg/L produced the highest efficiency of somatic embryo formation and shoot organogenesis. Rooting of embryogenic calli with adventitious shoots was done on solid MS medium containing 0.1 mg/L NAA and 0.3% activated carbon. Nearly 80% of embryogenic calli with shoot organogenesis could be rooted normal. Well-rooted plantlets were transferred into pots under a greenhouse condition, and plants derived from this system appeared phenotypically normal.

Keywords

References

  1. Ahn, D.K. 1998. Illustrated book of Korean medicinal herbs. Kyohak Publishing Co. Ltd., Korea. p. 134.
  2. Akashi, T., T. Furuno, T. Takahashi and S.I. Ayabe. 1994. Biosynthesis of triterpenoids in cultured cells, and regenerated and wild plant organs of Taraxacum officinale. Phytochemistry 36:303-308. https://doi.org/10.1016/S0031-9422(00)97065-1
  3. Bisset, N.G., J.D. Phillipson, F.C. Czygan, D. Frohne, D. Höltzel, A. Nagell, H.J. Pfander, G. Willuhn and W. Buff (eds.). 1994. Herbal drugs and phytopharmaceuticals: A Handbook for Practice on a Scientific Basis. CRC Press, Boca Raton, Ann Arbor, London, Tokyo. pp. 486-489.
  4. Duncan, D.B. 1955. Multiple range and multiple F tests. Biometrics 11:1-42. https://doi.org/10.2307/3001478
  5. Hall, R.D. 1991. The initiation and maintenance of plant cell suspension cultures. Plant Tiss. Cult. Manual. A3:1-21.
  6. Hernandez-Galicia, E., A. Aguilar-Contreras, L. Aguilar-Santamaria, R. Roman-Ramos, A.A. Chavez-Miranda, L.M. Garcia-Vega, J.L. Flores-Saenz and F.J. Alarcon-Aguilar. 2002. Studies on hypoglycemic activity of Mexican medicinal plants. Proc. West Pharmacol. Soc. 45:118-124.
  7. Kang, M.J. and K.S. Kim. 2001. Current trends of research and biological activities of dandelion. Food Industry Nutr. 6:60-67.
  8. Koo, H.N., S.H. Hong, B.K. Song, C.H. Kim, Y.H. Yoo and H.M. Kim. 2004. Taraxacum offcinale induces cytotoxicity through $TNF-{\alpha}$ and $IL-1{\alpha}$ secretion in Hep G2 cells. Life Sci. 74:1149-1157. Doi: 10.1016/j.lfs.2003.07.030.
  9. Lee, H.H., Y.S. Kim and H.Y. Park. 2007. Plant regeneration via organogenesis from leaf explant culture of Taraxacum coreanum Nakai. Korean J. Medicinal Crop Sci. 15(1):62-66 (in Korean).
  10. Lee, M.H., E.S. Yoon, J.H. Jeong and Y.E. Choi. 2004. Agrobacterium rhizogenes-mediated transformation of Taraxacum platycarpum and changes of morphological characters. Plant Cell Rep. 22:822-827. Doi:10.1007/s00299-004-0763-5.
  11. Lee, M.H., E.S. Yoon, S.J. Jung, K.H. Bae, J.W. Seo and Y.E. Choi. 2002. Plant regeneration and effect of auxin and cytokinin on adventitious shoot formation from seedling explant of Taraxacum platycarpum. Korean J. Plant Biotechnol. 29(2):111-115 (in Korean). https://doi.org/10.5010/JPB.2002.29.2.111
  12. Leu, Y.L., Y.L. Wang, S.C. Huang and L.S. Shi. 2005. Chemical constituents from roots of Taraxacum formosanum. Chem. Pharm. Bull. 53(7):853-855. Doi:10.1248/cpb.53.853.
  13. Lian, Y.J., G.Z. Lin, D.I. Yoo, W.B. Kim and X.M. Zhao. 2009. Effect of cytokinin on adventitious shoot formation and plant regeneration from explants of Pulsatilla koreana NAKAI. J. Plant Biotechnol. 36(2):170-173 (in Korean). https://doi.org/10.5010/JPB.2009.36.2.170
  14. Murashige, T. and F. Skoog. 1962. A revised medium for rapid growth and bio assays with tobacco tissue culture. Physiol. Plant. 15:473-497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  15. Paul, N.C., W.K. Kim, S.K. Woo, M.S. Park and S.H. Yu. 2006. Diversity of endophytic fungi associated with Taraxacum coreanum and their antifungal activity. Mycobiology 34(4): 185-190. https://doi.org/10.4489/MYCO.2006.34.4.185
  16. Schütz, K., R. Carlea and A. Schieber. 2006. Taraxacum--A review on its phytochemical and pharmacological profile. J. Ethnopharmacol. 107:313-323. Doi:10.1016/j.jep.2006.07.021.
  17. Sun, Y.L. and S.K. Hong. 2010. Establishment of a novel plant regeneration system from suspension-derived callus in the halophytic Leymus chinensis (Trin.). J. Plant Biotechnol. 37(2):228-235. Doi: 10.5010/JPB.2010.37.2.228.
  18. Sweeney, B., M. Vora, C. Ulbricht and E. Basch. 2005. Evidence-based systematic review of dandelion (Taraxacum officinale) by natural standard research collaboration. J. Herb. Pharmacother. 5(1):79-93. https://doi.org/10.1080/J157v05n01_09
  19. Wolbis, M., M. Królikowska and P. Bednarek. 1993. Polyphenolic compounds in Taraxacum officinale. Acta Pol. Pharm. Drug Res. 50:153-158.
  20. Yang, D.S., W.K. Whang and I.H. Kim. 1996. The constituents of Taraxacum hallaisanensis roots. Arch. Pharm. Res. 19(6):507-513. Doi:10.1007/BF02986020.