• Title/Summary/Keyword: N-Triple

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Determination of Vitamin B12 (Cyanocobalamin) in Fortified Foods by HPLC

  • Park, Youn-Ju;Jang, Jae-Hee;Park, Hye-Kyung;Koo, Yong-Eui;Hwang, In-Kyeong;Kim, Dai-Byung
    • Preventive Nutrition and Food Science
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    • v.8 no.4
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    • pp.301-305
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    • 2003
  • This study was conducted to develop an HPLC method for determining vitamin B$_{12}$ in fortified foods which has typically been determined by microbiological assays according to AOAC and Korean Food Code approved methods. Vitamin B$_{12}$ (cyanocobalamin) was determined by reversed-phase HPLC with a triple column and UV/VIS dectector (550 nm) using the column switching technique after extraction with 5 mM potassium phosphate solution by sonication without a clean-up procedure. The recovery of spiked samples and limit of detection (LOD) by HPLC were 78.6 ∼107.5 % and 2 ppb ($\mu\textrm{g}$/kg), respectively. The LOD of the microbiological assay (MBA) was much lower than that of HPLC. The concentrations of vitamin B$_{12}$ analyzed in all tested samples (n=12) confirmed compliance with declared label claims. The range of recovery ratio by the HPLC method when compared to the microbiological assay was 76.2 ∼140.0 %. There was not significant difference between the HPLC and MBA methods (p < 0.01) with r=0.9791 and linear regression y=0.9923x-0.04. The HPLC method for determining vitamin B$_{12}$ using the column-switching technique appears to be suitable for determining vitamin B$_{12}$ concentrations above 1 $\mu\textrm{g}$/100 g in fortified foods.ied foods.

A Process Analysis on the Experiences of Preliminary Child Care Teachers in Child Care Practice (예비보육교사의 실습 경험에 관한 과정 분석)

  • You, Byoungye;Yang, Sungeun
    • Korean Journal of Childcare and Education
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    • v.9 no.3
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    • pp.95-119
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    • 2013
  • The study uses a qualitative research method for an in-depth study on child care practice for preliminary child care teachers. The study shows firstly that prior to practice, a preliminary child care teacher expects to perform a dual role. The preliminary child care teacher conceives an unequal, vertical relationship with a practice guidance teacher and serves as someone between a teacher and a friend to children, while perceiving the facility head as a mediator and practice colleagues as companions. Secondly, the preliminary child care teacher performed a triple role, while the practice guidance teacher played the role of a preliminary child care teacher. A preliminary child care teacher has mutually supportive interaction with a practice guidance teacher and serves as someone between a teacher and a friend, while acting as an important supporter to the facility head and as an uncomfortable competitor to a practice colleague. Thirdly, when a practice is completed, a preliminary child care teacher feels satisfied and sorry, while emphasizing a balance between theory and practice in a curriculum. The purpose of the current study lies in figuring out the needs for child care practice for a preliminary child care teacher who is the subject in child care practice to suggest improvements for a qualitative enhancement of child care practice.

Single Crystal Growth of GaAs by Single Temperature Zone horizontal Bridgman(1-T HB) Method (단일 온도대역 수평 Bridgman(1-T HB) 법에 의한 GaAs 단결정 성장)

  • 오명환;주승기
    • Korean Journal of Crystallography
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    • v.7 no.1
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    • pp.73-80
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    • 1996
  • The single crystal growth has been carried out with the newly designed 1-T HB(single temperature zone horizontal Bridgman) system for GaAs crystals of 2 inch diameter doped with Si, Zn or undoped. With this method, incidence probability of single crystallinity was shown to be 0.73. Lattice defects evaluated from EPD(etch pit density) measurement were in the range of 5,000-20,000/cm2, dependent upon the doping condition. For the undoped GaAs crystals, carrier concentrations from the Hall measurement were ∼1×1016/cm3 at the seed part, which were less than half the concentrations of double of triple temperature zone(2-T, 3-T) HB grown crystals. By the 1-T HB method, therefore, GaAs crystals can be grown successfully with better yield and higher purity.

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A Study on the Design with RDF Authoring Tool for Metadata Management (메타데이터 관리를 위한 RDF 저작도구 설계에 관한 연구)

  • 최호찬;김차종
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.8 no.3
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    • pp.605-613
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    • 2004
  • Recently, the semantic web stands out in the next generation web. To realize the semantic web, the metadata integration is required between different metadatas on the web for metadata management. Otherwise, the integrated structure is needed to accommodate all of semantic, syntax and structure. RDF is one of the core technology and is more efficient framework for technology and interchange of metadata in the web, In this paper, for metadata management, we designed and implemented the RDF authoring system which converts each of metadata into RDF and makes it easy to manipulate and manage the different metadatas. For this, we researched about the RDF creation using Dublin Core metadata, the conversion of XML document into RDF, the RDF expression by N-Triple form and the integration of WSDL and RDF, and implemented the system on the lava platform. Basically, users using this system can integrate and manage metadatas easily even if they are not expert.

MNDO Studies on Intramolecular Proton Transfer Equilibria of Acetamide and Methyl Carbamate$^1$

  • Lee, Ik-Choon;Kim, Chang-Kon;Seo, Heon-Su
    • Bulletin of the Korean Chemical Society
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    • v.7 no.5
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    • pp.395-399
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    • 1986
  • Intramolecular proton transfer equilibria of acetamide and methyl carbamate have been studied theoretically by MNDO MO method. For both substrates, carbonyl-O protonated tautomer was found to be the most stable form, the next most stable one being N-protonated form. Gas phase proton transfers take place by the 1,3-proton rearrangement process and in all cases have prohibitively high activation barriers. When however one solvate water molecule participates in the process, the barriers are lowered substantially and the process proceeds in an intermolecular manner through the intermediacy of the water molecule via a triple-well type potential energy surface; three wells correspond to reactant(RC), intermediate(IC) and product complex(PC) of proton donor-acceptor pairs whereas two transition states(TS) have proton-bridge structure. General scheme of the process can be represented for a substrate with two basic centers(heteroatoms) of A and B as, $$ABH\limits^+\;+\;H_2O\;{\to}\;ABH\limits^+{\cdots}{\limits_{RC}}OH_2\;{\to}\;AB{\cdots}H\limits_{TS}^+{\cdots}{\limits_{1}}OH_2\;{\to}\;AB{\cdots}{\limits_{IC}}H\limits^+OH_2\;{\to}\;BA{\cdots}H\limits_{TS}^+{\cdots}{\limits_{2}}OH_2\;{\to}\;BA H\limits^+{\cdots}{\limits_{PC}}OH_2\;{\to}\;BAH\limits^+\;+\;H_2O$$ Involvement of a second solvate water had negligible effect on the relative stabilities of the tautomers but lowered barrier heights by 5∼6 Kcal/mol. It was calculated that the abundance of the methoxy-O protonated tautomer of the methyl carbamate will be negligible, since the tautomer is unfavorable thermodynamically as well as kinetically. Fully optimized stationary points are reported.

Motivation Versus Intention of Sharing Fake News Among Social Media Users during the Pandemic - A SEM Model

  • Alvi, Irum;Saraswat, Niraja
    • Journal of Contemporary Eastern Asia
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    • v.20 no.2
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    • pp.40-62
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    • 2021
  • Are intentions important in deciding the outcome of an action such as sharing misinformation among social media users during the pandemic? What is their role and how far they are important for the very act of fake sharing news? The social media users' actions on the social platform are determined by what they plan to do themselves; however, their motivation has an immense role to play in the dissemination of fake news on social media. The study proposes a conceptual model for understanding how select factors affect fake news sharing motivation and intentions of social media users. The study scrutinizes the relationship between content and context, fear of missing out (FoMO), news verification and news sharing gratification on the motivation and intention of social media users of networked Asian society. Empirical Data were drawn from social media users (N = 243) from India, using an online questionnaire based on prior studies and structural equation modeling (SEM) approach was used to analyze the data collected. Results indicate that news content, news verification, and news sharing gratification have a direct and positive relationship with sharing motivation. On the other hand, news context and content, FoMO and news sharing gratification have a positive significant relationship with sharing intention. Likewise, it was discovered that news verification will decrease sharing intention of the social media users. However, news context, that is the pandemic in the case of the present study and FoMO were not identified as determinant variables for sharing motivation among social media users. The research limitations and further scope were discussed.

Effect of hydrogel-based antibiotic intracanal medicaments on crown discoloration

  • Rayan B. Yaghmoor;Jeffrey A. Platt;Kenneth J. Spolnik;Tien Min Gabriel Chu;Ghaeth H. Yassen
    • Restorative Dentistry and Endodontics
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    • v.46 no.4
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    • pp.52.1-52.11
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    • 2021
  • Objectives: This study evaluated the effects of low and moderate concentrations of triple antibiotic paste (TAP) and double antibiotic paste (DAP) loaded into a hydrogel system on crown discoloration and explored whether application of an adhesive bonding agent prevented crown discoloration. Materials and Methods: Intact human molars (n = 160) were horizontally sectioned 1 mm apical to the cementoenamel junction. The crowns were randomized into 8 experimental groups (calcium hydroxide, Ca[OH]2; 1, 10, and 1,000 mg/mL TAP and DAP; and no medicament. The pulp chambers in half of the samples were coated with an adhesive bonding agent before receiving the intracanal medicament. Color changes (ΔE) were detected by spectrophotometry after 1 day, 1 week, and 4 weeks, and after 5,000 thermal cycles, with ΔE = 3.7 as a perceptible threshold. The 1-sample t-test was used to determine the significance of color changes relative to 3.7. Analysis of variance was used to evaluate the effects of treatment, adhesive, and time on color change, and the level of significance was p < 0.05. Results: Ca(OH)2 and 1 and 10 mg/mL DAP did not cause clinically perceivable tooth discoloration. Adhesive agent use significantly decreased tooth discoloration in the 1,000 mg/mL TAP group up to 4 weeks. However, adhesive use did not significantly improve coronal discoloration after thermocycling when 1,000 mg/mL TAP was used. Conclusions: Ca(OH)2 and 1 and 10 mg/mL DAP showed no clinical discoloration. Using an adhesive significantly improved coronal discoloration up to 4 weeks with 1,000 mg/mL TAP.

Development of Economic Culture System Using Wastewater for Microalgae in Winter Season (폐수를 이용한 겨울철 경제적 미세조류 배양 시스템의 개발)

  • Lee, Sang-Ah;Lee, Changsoo;Lee, Seung-Hoon;An, Kwang-Guk;Oh, Hee-Mock;Kim, Hee-Sik;Ahn, Chi-Yong
    • Korean Journal of Environmental Biology
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    • v.32 no.1
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    • pp.58-67
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    • 2014
  • The outdoor mass cultivation is not possible for microalgae in Korea all year round, due to cold winter season. It is not easy to maintain proper level of productivity of microalgae even in winter. To prevent a drastic decrease of temperature in a greenhouse, two layers were covered additionally, inside the original plastic layer of the greenhouse. The middle layer was made up of plastic and the inner layer, of non-woven fabric. Acrylic transparent bioreactors were constructed to get more sunlight, not only from the upper side but also from the lateral and bottom directions. In winter at freezing temperatures, six different culture conditions were compared in the triply covered, insulated greenhouse. Wastewater after anaerobic digestion was used for the cultivation of microalgae to minimize the production cost. Water temperature in the bioreactors remained above $10^{\circ}C$ on average, even without any external heating system, proving that the triple-layered greenhouse is effective in keeping heat. Algal biomass reached to 0.37g $L^{-1}$ with the highest temperature, in the experimental group of light-reflection board at the bottom, with nitrogen and phosphorus removal rate of 92% and 99%, respectively. When fatty acid composition was analyzed using gas-chromatography, linoleate (C18 : 3n3) occupied the highest proportion up to 61%, in the all experiment groups. Chemical oxygen demand (COD), however, did not decrease during the cultivation, but rather increased. Although the algal biomass productivity was not comparable to warm seasons, it was possible to maintain water temperature for algae cultivation even in the coldest season, at the minimum cost.

Development of Greenhouse Cooling and Heating Load Calculation Program Based on Mobile (모바일 기반 온실 냉난방 부하 산정 프로그램 개발)

  • Moon, Jong Pil;Bang, Ji Woong;Hwang, Jeongsu;Jang, Jae Kyung;Yun, Sung Wook
    • Journal of Bio-Environment Control
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    • v.30 no.4
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    • pp.419-428
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    • 2021
  • In order to develope a mobile-based greenhouse energy calculation program, firstly, the overall thermal transmittance of 10 types of major covers and 16 types of insulation materials were measured. In addition, to estimate the overall thermal transmittance when the cover and insulation materials were installed in double or triple layers, 24 combinations of double installations and 59 combinations of triple installations were measured using the hotbox. Also, the overall thermal transmittance value for a single material and the thermal resistance value were used to calculate the overall thermal transmittance value at the time of multi-layer installation of covering and insulating materials, and the linear regression equation was derived to correct the error with the measured values. As a result of developing the model for estimating thermal transmittance when installing multiple layers of coverings and insulating materials based on the value of overall thermal transmittance of a single-material, the model evaluation index was 0.90 (good when it is 0.5 or more), indicating that the estimated value was very close to the actual value. In addition, as a result of the on-site test, it was evaluated that the estimated heat saving rate was smaller than the actual value with a relative error of 2%. Based on these results, a mobile-based greenhouse energy calculation program was developed that was implemented as an HTML5 standard web-based mobile web application and was designed to work with various mobile device and PC browsers with N-Screen support. It had functions to provides the overall thermal transmittance(heating load coefficient) for each combination of greenhouse coverings and thermal insulation materials and to evaluate the energy consumption during a specific period of the target greenhouse. It was estimated that an energy-saving greenhouse design would be possible with the optimal selection of coverings and insulation materials according to the region and shape of the greenhouse.

Climate-Smart Agriculture (CSA)-Based Assessment of a Rice Cultivation System in Gimje, Korea (한국 김제의 벼 경작 시스템의 기후스마트농업 (Climate-Smart Agriculture) 기반의 평가)

  • Talucder, Mohammad Samiul Ahsan;Kim, Joon;Shim, Kyo-Moon
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.23 no.4
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    • pp.235-250
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    • 2021
  • The overarching question of this study is how a typical rice cultivation system in Gimje, Korea was keeping up with the triple-win challenge of climate-smart agriculture (CSA). To answer this question, we have employed (1) quantitative data from direct measurement of energy, water, carbon and information flows in and out of a rice cultivation system and (2) appropriate metrics to assess production, efficiency, GHG fluxes, and resilience. The study site was one of the Korean Network of Flux measurement (KoFlux) sites (i.e., GRK) located at Gimje, Korea, managed by National Academy of Agricultural Science, Rural Development Administration. Fluxes of energy, water, carbon dioxide (CO2) and methane (CH4) were directly measured using eddy-covariance technique during the growing seasons of 2011, 2012 and 2014. The production indicators include gross primary productivity (GPP), grain yield, light use efficiency (LUE), water use efficiency (WUE), and carbon uptake efficiency (CUE). The GHG mitigation was assessed with indicators such as fluxes of carbon dioxide (FCO2), methane (FCH4), and nitrous oxide (FN2O). Resilience was assessed in terms of self-organization (S), using information-theoretic approach. Overall, the results demonstrated that the rice cultivation system at GRK was climate-smart in 2011 in a relative sense but failed to maintain in the following years. Resilience was high and changed little for three year. However, the apparent competing goals or trade-offs between productivity and GHG mitigation were found within individual years as well as between the years, causing difficulties in achieving the triple-win scenario. The pursuit of CSA requires for stakeholders to prioritize their goals (i.e., governance) and to practice opportune interventions (i.e., management) based on the feedback from real-time assessment of the CSA indicators (i.e., monitoring) - i.e., a purpose-driven visioneering.