• 농업과학연구
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• 제17권2호
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• pp.77-81
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• 1990

Chitin 합성 저해제인 buprofezin이 P. ostreatus 및 P. sajor-caju 균사의 생장과 원형질체 나출 및 재생에 미치는 영향을 조사한 바를 요약하면 buprofezin은 P. ostreatus 및 P. sajor-caju.의 균사생장을 심히 저해하였고 buprofezin의 첨가농도가 높을수록 균사생장 억제가 심하였다. Buprofezin 처리에 의하여 기균사가 증가하였으나 균사의 형태에는 변화가 없었다. 원형질체의 나출량은 buprofezin 200-500 ppm 첨가구의 균사에서 무처리보다 현저히 높은 결과를 보였고, 원형질체의 재생율은 buprofezin의 처리에 의하여 증가하였다.

• Mycobiology
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• 제47권4호
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• pp.506-511
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• 2019

We investigated the antifungal activities of an endophytic fungus identified as Acaromyces ingoldii, found on a loblolly (Pinus taeda L.) pine bolt in Louisiana during routine laboratory microbial isolations. The specific objectives were to determine the inhibitory properties of A. ingoldii secondary metabolites (crude extract) on the mycelial growth of a brown-rot fungus Gloeophyllum trabeum and a white-rot fungus Trametes versicolor, and to determine the effective concentration of A. ingoldii crude preparation against the two decay fungi in vitro. Results show the crude preparation of A. ingoldii from liquid culture possesses significant mycelial growth inhibitory properties that are concentration dependent against the brownrot and white-rot fungi evaluated. An increase in the concentration of A. ingoldii secondary metabolites significantly decreased the mycelial growth of both wood decay fungi. G. trabeum was more sensitive to the inhibitory effect of the secondary metabolites than T. versicolor. Identification of specific A. ingoldii secondary metabolites, and analysis of their efficacy/specificity warrants further study. Findings from this work may provide the first indication of useful roles for Acaromyces species in a forest environment, and perhaps a future potential in the development of biocontrol-based wood preservation systems.

• Mycobiology
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• 제35권2호
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• pp.76-81
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• 2007

Lactobacillus casei KC-324 was tested for its ability to inhibit aflatoxin production and mycelial growth of Aspergillus flavus ATCC 15517 in liquid culture. flatoxin $B_{1}$ biosynthesis and mycelial growth were inhibited in both simultaneous culture and individual antagonism assays, suggesting that the inhibitory activity was due to extracellular metabolites. produced in cell-free supernatant fluids of the cultured broth of L. casei KC-324. In cell-free supernatant fluids of all media tested, deMan, Rogosa and Sharpe broth, potato dextrose broth, and Czapek-Dox broth+1% yeast extract showed higher antiaflatoxigenic activity. In these case, fungal growths, however, was not affected as measured by mycelial dry weight. The antiaflatoxigenic metabolites from L. casei KC-324 were produced over wide range of temperatures between $25^{\circ}C$ and $37^{\circ}C$. However, these metabolites were not thermostable since the inhibitory activity of the supernatant was inactivated within 30 minutes at $100^{\circ}C$ and $121^{\circ}C$. The inhibitory activity was not influenced by changing pH of supernatant between 4 and 10. However, the antiaflatoxigenic activity was slightly reduced at pH 10.

• Mycobiology
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• 제37권4호
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• pp.277-285
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• 2009

In this study, dual culture, poison agar, and direct methods were used to assess the ability of Trichoderma virens IMI-392430, T. pseudokoningii IMI-392431, T. harzianum IMI-392432, T. harzianum IMI-392433, and T. harzianum IMI-392434 to control Ceratocystis paradoxa, which causes the pineapple disease of sugarcane. The highest percentage inhibition of radial growth (PIRG) values were observed with T. harzianum IMI-392432 using two dual culture methods, 63.80% in Method I and 80.82% in Method II. The minimum colony overgrowth time was observed with T. harzianum IMI-392432 and the maximum was observed with T. pseudokoningii IMI-392431. Different concentrations of different day-old metabolites of Trichoderma isolates were tested against mycelial growth of C. paradoxa. The highest PIRG (84.685%) exhibited at 80% concentration of 30-day-old metabolites of T. harzianum IMI-392432 using the modified bilayer poison agar method. In the direct assay method the maximum mycelial growth weight (PIGW) was observed at the same concentration and the same day-old metabolites of T. harzianum IMI-392432. This study showed that Trichoderma isolates have a good antagonistic effect on C. paradoxa mycelial growth and T. harzianum IMI-392432 has the most potential to control the pineapple disease pathogen.

• Mycobiology
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• 제28권4호
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• pp.190-192
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• 2000

Antifungal bacteria for biological control of plant diseases or production of novel antibiotics to plant pathogens were isolated in 1997 from various soils of Ansung, Chunan, Koyang, and Paju in Korea. Sixty-four bacterial strains pre-screened from approximately 1,400 strains were tested on V-8 juice agar against eight plant pathogenic fungi using in vitro bioassay technique for inhibition of mycelial growth. Test pathogens were Alternaria mali, Colletotrichum gloeosporioides, C. orbiculare, Fusarium oxysporum f. sp. cucumerinum, F. oxysporum f. sp. lycopersici, Magnaporthe grisea, Phytophthora capsici, and Rhizoctonia solani. A wide range of antifungal activity of bacterial strains was found against the pathogenic fungi, and strain RC-B77 showed the best antifungal activity. Correlation analysis between inhibition of each fungus and mean inhibition of all eight fungi by 64 bacterial strains revealed that C. gloeosporioides would be best appropriate for detecting bacterial strains producing antibiotics with potential as biocontrol agents for plant pathogens.

• Journal of Microbiology and Biotechnology
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• 제5권6호
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• pp.341-345
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• 1995

Seventy four microorganisms, which have antagonistic activity against to Fusarium culmorum, were tested for their inhibitory effect on colony development of obligate biotroph Erysiphe graminis f. sp. hordei Marchal, the causal agent of powdery mildew on barley plants. Of these, 13 actinomycetes isolates were shown to reduce the colony development of mildew completely by application of their 10% cell-free culture filtrates on barley leaves. An Isolate, A252, was the most powerful antagonist and its antifungal activity was further assessed. The colony development of mildew was significantly reduced by application of the 1% cell-free culture filtrate of isolate A252. In comparison to the control, the protective and curative application of 10% cell-free culture filtrate from A252 showed 88.5% and 96.1% reduction of colony numbers respectively. By the protective application, 68.3% of the inhibition was observed after 9 days of treatment, thus showed prolonged inhibitory effect. In vitro test, complete inhibition of the mycelial growth of Microdochium nivale was achieved by the treatment of 1% A252 culture filtrate and 80.2% of inhibition was observed by the 0.1% treatment.

• 한국버섯학회지
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• 제11권1호
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• pp.24-31
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• 2013

선행연구로서 꽃송이버섯 배지내 LED 조명색에 의한 푸른곰팡이 생육억제 효과는 모든 처리구에서 생육이 억제되었으나 blue 처리구에서 푸른곰팡이균의 포자발생량이 매우 높아 꽃송이버섯 배양에 부적합하다는 것으로 확인되었다. 배양 28일 후 LED 파장에 따른 꽃송이버섯의 균사생장 특성과 LED 파장 색상별 배양시간의 경과에 따른 균사생장 특성을 조사한 결과 모두 red 파장 처리구에서 균사생장이 높은 경향을 나타내었다. 이에 따라 red 파장 범위에서 광량을 달리하여 균사생장 조건을 확인한 결과, 광량 세기가 낮은 1.41 ${\mu}mol/m^2S$ 처리구에서 우수한 균사생장을 나타내었다. 계대배양 실시 여부에 따른 균사생장 특성에서는 실험 전 1회 계대배양을 실시한 처리구에서 유의적인 차이를 나타내었다. 배지조성을 달리한 톱밥배지 내 LED 광량별 균사생장은 미송 처리구에서 높은 경향을 나타내었다. 이를 미송 톱밥배지에서 LED red 2.11 ${\mu}mol/m^2S$의 광량을 처리하였을 경우 84일 배양한 처리구에서 유의적인 차이를 보였다. 상기의 결과를 종합하여 보았을 경우 red 파장 2.11 ${\mu}mol/m^2S$ 광량에서 84일 이상 미송 톱밥배지에서 배양하는 것이 꽃송이버섯 배양에 좋을 것으로 판단되었다.

• The Plant Pathology Journal
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• 제36권2호
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• pp.133-147
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• 2020

Hexanal, a C-6 aldehyde has been implicated to have antimicrobial properties. Hence, this study was conducted to determine the antifungal activities of hexanal vapor against major postharvest pathogens of banana viz., Colletotrichum gloeosporioides and Lasiodiplodia theobromae. The pathogens were cultured in vitro and exposed to hexanal vapor at 600, 800, 1,000 and 1,200 ppm. Mycelial growth of both fungal pathogens were inhibited completely at 800 ppm and the incidence of anthracnose and stem-end rot diseases reduced by 75.2% and 80.2%, respectively. The activities of peroxidase, polyphenol oxidase, phenylalanine ammonia-lyase and glucanase had transiently increased in hexanal vapor treated banana by 5 to 7 days and declined thereafter. Postharvest treatment of banana with hexanal vapor resulted in phospholipase D inhibition and also resulted in cell wall thickening of the treated fruit, which impeded the penetration of the pathogenic spores. This was further confirmed by scanning electron micrographs. The defense-related protein intermediaries had increased in hexanal vapor treated banana fruit, which suggests induced resistance against C. gloeosporioides and L. theobromae, via., the phenylpropanoid pathway which plays a significant role in hindering the pathogen quiescence. Delayed ripening due to inhibition of phospholipase D enzyme, inhibition of mycelial growth and induced systemic resistance by defense enzymes collectively contributed to the postharvest disease reduction and extended shelf life of fruit.

• 대한약학회:학술대회논문집
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• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.1
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• pp.271.2-271.2
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• 2003

The ${\gamma}$- and ${\beta}$-secretase are one of the most important proteases, which cleave amyloid precursor protein (APP) into neurotoxic A${\beta}$ peptide in Azheimer's type dementia. In the course of screening for anti-dementia agents from natural products, the mycelial culture of mushroom Phellinus linteus showed potent inhibition againt ${\beta}$-secretase (BACE1). (omitted)

• 한국환경농학회지
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• 제37권1호
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• pp.34-40
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• 2018

탄저병이 발생한 자두에서 분리된 C. acutatum 6개와 C. gloeosporioides 5개 균주에 대한 benoomyl, prochloraz manganese complex, azoxystrobin, pyraclostrobin, tebuconazole 및 dithianon의 균사생장억제효과와 포자발아억제효과를 in vitro에서 검정하였다. 포장 권장사용농도를 기준으로 비교하면, prochloraz manganese가 탄저병 균주의 균사생장을 가장 효과적으로 억제하였다. 포자발아억제에서는 dithianon과 pyraclostrobin의 효과가 가장 높았다. Benomyl, prochloraz manganese complex, azoxystrobin 및 tebuconazole은 포자의 발아를 억제하지는 않았으나, 발아 후 비정상적인 발아관의 신장을 유기하여 균사생장 억제하였다. 본 연구의 결과를 통해 자두 탄저병에 대하여 dithianon은 예방효과, prochloraz manganese, azoxystrobin, tebuconazole 등은 치료효과, pyraclostrobin은 예방 및 치료효과가 높은 약제로 판단되며, 포장 적용시험을 통해 자두의 탄저병을 효과적으로 방제할 수 있는 약제를 선발해야 할 것이다.