• KIEAE Journal
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• v.16 no.5
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• pp.5-11
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• 2016

Purpose: Residents in deteriorated residential areas of Seoul have suffered from parking shortage problem. Since it is almost impossible to supply new parking, it is critical to find a way to efficiently utilize existing parking spaces. This study proposes utilizing unused parking spaces in apartment complexes so that residents in multi-unit or multi-family houses can share those parking spaces. Method: Spatial scope of this research is limited to multiplex-clustered neighborhood and apartment complex in Seoul. To identify the nature of parking problem, we reviewed current parking situations in residential areas and policies from the Seoul Metropolitan Government. To understand the amount of parking spaces required to solve parking shortages in multiplex-clustered neighborhoods, this study analyzed car ownership per household and available parking spaces in old residential areas. Then we were able to apprehend the amount of parking spaces that could be used for residents in aged multiplex-clustered areas. Result: Our analysis shows a great potential to utilize surplus parking spaces from apartment complexes for solving parking problems in multiplex-clustered neighborhoods.

• Journal of Microbiology and Biotechnology
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• v.14 no.4
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• pp.737-744
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• 2004

A multiplex polymerase chain reaction (PCR) was developed for the simultaneous detection and differentiation among Vairimorpha spp. and Nosema spp. and identification of Vairimorpha necatrix from Lepidoptera insects. Three sets of primers were selected from different genomic sequences to specifically amplify an 831 bp amplicon within the SSU rRNA gene, specific for both Vairimorpha spp. and Nosema spp. (MSSR primer); a 542 bp amplicon within the SSU rRNA gene, specific for Vairimorpha spp. (VSSU primer); and a 476 bp amplicon within the actin gene, specific for Vairimorpha necatrix (VNAG primer). Using the primers in conjunction with multiplex PCR, it was possible to detect Vairimorpha spp. and Nosema spp. and to differentiate between them. The sensitivity of this PCR assay was approximately 10 spores per milliliter. It is proposed that the multiplex PCR is a sensitive, specific, and rapid tool that can serve as a useful differential diagnostic tool for detecting Vairimorpha spp. and Nosema spp. in Lepidoptera insect.

• Korean Journal of Veterinary Service
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• v.37 no.4
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• pp.247-252
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• 2014

The economic impact of swine mycoplasma infection is high. An accurate diagnosis is often difficult and time consuming. We report the development and validation of an effective multiplex polymerase chain reaction (PCR) assay that detects Mycoplasma (M.) hyopneumoniae and M. hyorhinis. The multi detection of M. hyopneumoniae and M. hyorhinis primer set were employed to detect mycoplasma species and typing of the species was performed on the basis of sequence analysis of the PCR product. The target nucleic acid fragments were specifically amplified by M. hyopneumoniae and M. hyorhinis PCR with 16S ribosomal DNA primers. Single and mixed Mycoplasma species DNA templates were used to evaluate the specificity of the multiplex assay. The corresponding specific DNA products were amplified for each pathogen. The multiplex PCR assay provides a novel tool for simultaneous detection and differentiation of M. hyopneumoniae and M. hyorhinis.

• KIEAE Journal
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• v.16 no.4
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• pp.21-30
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• 2016

Purpose: Energy saving in the built facilities is getting important due to energy crisis. The Korea government has been implemented several energy and green building policies and practices. The both of government and industry also developed green building strategies ant technologies to reduce energy consumption and carbon emission. The purpose of this research is to identify applicable green building strategies and technologies for that can be cost effective and applicable to a multiplex house. Method: This research identified appropriate green building strategies from analysing green building strategies from G-SEED certified apartment projects and popular green building strategies. This study also adopted a survey research method to find out the applicable green building strategies for a multiplex housing. In addition, this research also conduct cost estimating to identify initial cost premium of green building strategies. Results: The research outcomes in this study guide a building owner to know about initial cost premiums of green building strategies and technologies and an architect and contractor to identify appropriate and cost effective green building strategies that can be applicable to a multiplex house.

• Proceedings of the Korean Vacuum Society Conference
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• 2013.08a
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• pp.276-276
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• 2013

Here we report an integrated microdevice consisting of an efficient passive mixer, a magnetic separation chamber, and a capillary electrophoretic microchannel in which DNA barcode assay, target pathogen separation, and barcode DNA capillary electrophoretic analysis were performed sequentially within 30 min for multiplex pathogen detection at the single-cell level. The intestine-shaped serpentine 3D micromixer provides a high mixing rate to generate magnetic particle-pathogenic bacteria-DNA barcode labelled AuNP complexes quantitatively. After magnetic separation and purification of those complexes, the barcode DNA strands were released and analyzed by the microfluidic capillary electrophoresis within 5 min. The size of the barcode DNA strand was controlled depending on the target bacteria (Staphylococcus aureus, Escherichia coli O157:H7, and Salmonella typhimurium), and the different elution time of the barcode DNA peak in the electropherogram allows us to recognize the target pathogen with ease in the monoplex as well as in the multiplex analysis. In addition, the quantity of the DNA barcode strand (~104) per AuNP is enough to be observed in the laser-induced confocal fluorescence detector, thereby making single-cell analysis possible. This novel integrated microdevice enables us to perform rapid, sensitive, and multiplex pathogen detection with sample-in-answer-out capability to be applied for biosafety testing, environmental screening, and clinical trials.

• Food Science and Biotechnology
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• v.17 no.4
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• pp.829-832
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• 2008

A multiplex polymerase chain reaction (PCR) method was developed to simultaneously detect 4 varieties of genetically modified (GM) cotton. The event-specific primers were used to distinguish the 4 varieties of GM cotton (MON1445, MON15985, MON88913, and LLcotton25) using multiplex PCR. The acyl carrier protein 1 (Acp1) gene was used as an endogenous reference gene of cotton in the PCR detection. The primer pair Acp1-AF/AR containing a 99 bp amplicon was used to amplify the Acp1 gene and no amplified product was observed in any of the 13 different plants used as templates. This multiplex PCR method allowed for the detection of event-specific targets in a genomic DNA mixture of up to 1% GM cotton containing MON1445, MON15985, MON88913, and LLcotton25.

• Parasites, Hosts and Diseases
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• v.60 no.4
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• pp.295-299
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• 2022

Malaria elimination and control require prompt and accurate diagnosis for treatment plan. Since microscopy and rapid diagnostic test (RDT) are not sensitive particularly for diagnosing low parasitemia, highly sensitive diagnostic tools are required for accurate treatment. Molecular diagnosis of malaria is commonly carried out by nested polymerase chain reaction (PCR) targeting 18S rRNA gene, while this technique involves long turnaround time and multiple steps leading to false positive results. To overcome these drawbacks, we compared highly sensitive cytochrome oxidase gene-based single-step multiplex reaction with 18S rRNA nested PCR. Cytochrome oxidase (cox) genes of P. falciparum (cox-III) and P. vivax (cox-I) were compared with 18S rRNA gene nested PCR and microscopy. Cox gene multiplex PCR was found to be highly specific and sensitive, enhancing the detection limit of mixed infections. Cox gene multiplex PCR showed a sensitivity of 100% and a specificity of 97%. This approach can be used as an alternative diagnostic method as it offers higher diagnostic performance and is amenable to high throughput scaling up for a larger sample size at low cost.

• Proceeding of Spring/Autumn Annual Conference of KHA
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• 2006.11a
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• pp.316-321
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• 2006

Multiplex use facility emphasized the importance of indoor air environment because many people using that place. People using parking lot of multiplex use facility in little time, but a pollutant of in indoor air menacing user health. This study measure and analyze the CO, $CO_2$ for better air quality of an indoor parking lot in the multiplex user facility. As a result, two-way opened above ground parking lot is higher numerical value of CO, $CO_2$ than an underground parking lot. And a parking lot of square form is higher numerical value of $CO_2$ than a parking lot of rectangle form.

• Proceedings of the Korean Institute of Interior Design Conference
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• 2006.05a
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• pp.158-164
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• 2006

Multiplex have many screen in one place and provide customer Multiple Entertainment Option. Today multiplex cinema is a new culture place for the public to approach most easily in Korea. It also gave a chance to recognize how important a popular culture place is. but it has a problem that this space wasn't designed for customer and don't have even a rest area for them as they focused only on economic growth. therefore, in this study, it point's the utilization of Service Areas problems and provide basic data of interior design for customer.

• Biomedical Science Letters
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• v.13 no.1
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• pp.71-73
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• 2007

For the rapid detection of objective pathogenic bacteria from colon biopsy specimens, multiplex PCR (polymerase chain reaction) method was developed. The major objective bacteria in this study were Shiga-like toxin producing E. coli O157:H7, Staphylococcus aureus, Vibrio parahaemolyticus, Listeria monocytogenes, Shigella spp. Salmonella spp. and Yersinia spp.. To detect simultaneously 7 kinds of pathogenic bacteria in single reaction tube, multiplex PCR system was executed using 6 sets of primers used in single PCR system for the respective bacteria. The results in this research might be applied for the detection of pathogenic bacteria form colon biopsy samples.