Multiplex PCR Assay from Colon Biopsy Specimens in Acute Infectious Colitis Patients

  • Lee, Gyu-Sang (Department of Biomedica Laboratory Science, College of Health Science, Yonsei University) ;
  • Lim, Kwan-Hun (Department of Biomedica Laboratory Science, College of Health Science, Yonsei University) ;
  • Kim, Jong-Bae (Department of Biomedica Laboratory Science, College of Health Science, Yonsei University)
  • ;
  • ;
  • 김종배 (연세대학교 보건과학대학 임상병리학과 임상미생물학실험실)
  • Published : 2007.03.31

Abstract

For the rapid detection of objective pathogenic bacteria from colon biopsy specimens, multiplex PCR (polymerase chain reaction) method was developed. The major objective bacteria in this study were Shiga-like toxin producing E. coli O157:H7, Staphylococcus aureus, Vibrio parahaemolyticus, Listeria monocytogenes, Shigella spp. Salmonella spp. and Yersinia spp.. To detect simultaneously 7 kinds of pathogenic bacteria in single reaction tube, multiplex PCR system was executed using 6 sets of primers used in single PCR system for the respective bacteria. The results in this research might be applied for the detection of pathogenic bacteria form colon biopsy samples.

Keywords