Biomedical Science Letters (대한의생명과학회지)

The Korean Society for Biomedical Laboratory Sciences (대한의생명과학회)
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Multiplex PCR Assay from Colon Biopsy Specimens in Acute Infectious Colitis Patients

  • Lee, Gyu-Sang (Department of Biomedica Laboratory Science, College of Health Science, Yonsei University) ;
  • Lim, Kwan-Hun (Department of Biomedica Laboratory Science, College of Health Science, Yonsei University) ;
  • Kim, Jong-Bae (Department of Biomedica Laboratory Science, College of Health Science, Yonsei University)
  • ;
  • ;
  • 김종배 (연세대학교 보건과학대학 임상병리학과 임상미생물학실험실)
  • Published : 2007.03.31
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Abstract

For the rapid detection of objective pathogenic bacteria from colon biopsy specimens, multiplex PCR (polymerase chain reaction) method was developed. The major objective bacteria in this study were Shiga-like toxin producing E. coli O157:H7, Staphylococcus aureus, Vibrio parahaemolyticus, Listeria monocytogenes, Shigella spp. Salmonella spp. and Yersinia spp.. To detect simultaneously 7 kinds of pathogenic bacteria in single reaction tube, multiplex PCR system was executed using 6 sets of primers used in single PCR system for the respective bacteria. The results in this research might be applied for the detection of pathogenic bacteria form colon biopsy samples.

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