Biomedical Science Letters (대한의생명과학회지)
- Volume 13 Issue 1
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- Pages.71-73
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- 2007
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- 1738-3226(pISSN)
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- 2288-7415(eISSN)
Multiplex PCR Assay from Colon Biopsy Specimens in Acute Infectious Colitis Patients
- Lee, Gyu-Sang (Department of Biomedica Laboratory Science, College of Health Science, Yonsei University) ;
- Lim, Kwan-Hun (Department of Biomedica Laboratory Science, College of Health Science, Yonsei University) ;
- Kim, Jong-Bae (Department of Biomedica Laboratory Science, College of Health Science, Yonsei University)
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- 김종배 (연세대학교 보건과학대학 임상병리학과 임상미생물학실험실)
- Published : 2007.03.31
Abstract
For the rapid detection of objective pathogenic bacteria from colon biopsy specimens, multiplex PCR (polymerase chain reaction) method was developed. The major objective bacteria in this study were Shiga-like toxin producing E. coli O157:H7, Staphylococcus aureus, Vibrio parahaemolyticus, Listeria monocytogenes, Shigella spp. Salmonella spp. and Yersinia spp.. To detect simultaneously 7 kinds of pathogenic bacteria in single reaction tube, multiplex PCR system was executed using 6 sets of primers used in single PCR system for the respective bacteria. The results in this research might be applied for the detection of pathogenic bacteria form colon biopsy samples.