• Journal of the Korean Society for Nondestructive Testing
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• v.29 no.5
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• pp.442-449
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• 2009

An investigation has been made on the relationship between characteristics of torsional mode signal in MsS and SH mode signal in BEM modeling for the defect of stainless steel pipe. In order to compare torsional mode signal with SH mode signal of defect in stainless steel pipe, specimens were made by changing size of depth and width along to circumferential direction 360 degrees. All the defects was detected by torsional mode signal of MsS, especially according to the change of depth size, amplitude of signal was changed. But width change for the circumferential defects has no certain tendency. SH mode signal of BEM modeling shows similar results with torsional mode, with change makes amplitude variation of signal. In this paper, the characteristics of torsional mode and SH mode signals were found. It is possible to predict the circumferential defects for the pipe by SH mode modeling.

• Proceedings of the Korean Society of Grassland Science Conference
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• 1999.06a
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• pp.75.1-75
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• 1999

제초제 저항성 형질전환 목초 생산을 위하여 유전자총(Gene-gun) 및 Agrobacterium 기법을 이용하여 형질전환을 시도한 결과를 요약하면 다음과 같다. 1. 공시재료 : 알팔파 (cv. Vernal, Anker), 오차드그라스 (합성19호) 2. 캘러스의 유도 및 증식 : 알팔파 종자를 SH-3 배지 (SH기본배지, 2,4-D 3mg/L 포함) 그리고 오차드그라스 종자를 MS-5 배지 (MS기본배지, 2,4-D 5mg/L, 2g casein hydrolysate 포함)에 치상하여 캘러스를 유도하였다. 유도된 캘러스는 증식을 위하여 SH-3 및 MS-3 배지 (MS기본배지, 2,4-D 3mg/L, 2g casein hydrolysate 포함)에서 2주 간격으로 subculture 하였다.(중략)

• Proceedings of the Korean Journal of Food and Nutrition Conference
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• 2001.12a
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• pp.123-123
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• 2001

주류는 미생물에 의한 효소작용으로 원료 성분이 분해됨으로서 생성되는 당분, 아미노산, 유기산등의 맛 성분과 발효과정 동안 생성되는 휘발성 성분들이 조화되어 상당히 다양한 성분들이 향미에 관여하므로 향기의 주성분 규명이나 향기성분을 이용한 품질의 규격화가 매우 어려운 문제로 보여진다. 술덧의 안전한 발효와 잡균오염을 방지하여 품질이 균일한 술을 생산할 목적으로 재래누룩과는 별도로 Aspergillus kawachii등의 균을 이용한 개량곡자(koji)가 술 제조시에 많이 이용되고 있으나 이러한 방법은 탁주의 대량생산과 수율은 증가시켰으나 다양한 품질이나 맛의 향상은 기대하기 어렵다. 본 실험은 재래누룩에서 분리된 곰팡이 10종과 대조균주로 Aspergillus kawachii CF1002 그리고 효모 Saccharomyces cerevisiae를 이용하여 술을 담금하여 그 제품의 headspace gas를 포집하여 GC/MS로 휘발성 향기성분을 분석하였으며 또한 관능평가를 실시하여 검토하였다. 각 균주별로 총 27∼40종의 향기성분이 확인되었으며 전체 시료에서 알콜(peak area% 52.3 9∼70.36%), 알칸류(3.49∼14.17%), 에스테르류(3.23∼7.37%), 유기산류(0∼2.85%), 알데히드류(0∼0.44%)의 순으로 함유량이 높은 것으로 나타났다. GC/MS를 통한 향기성분의 분석 결과 각각의 선발 균주로 제조된 술의 향기성분의 패턴이 서로 상이하였으며 이것은 통계분석에 의해 그 특징이 유의적으로 분석되어 Aspergillus sp. SH-607, Aspergillus sp. SH-613, Aspergillus sp. SH-667, Aspergillus sp. SH-696 그리고 Aspergillus sp. SH-412는 기호도가 높은 것으로 관찰되었다. 수집된 우수균주 10종과 Aspergillus kawachii등으로 담금하여 제조한 술 시료 11종류를 대상으로 하여 쓴맛, 미묘한 맛, 떫은 맛, 신맛, 좋은 맛의 정도를 5단계로 나누어 관능검사를 실시한 다음 자료를 Duncan's multiple range test로 분석한 결과 전체적으로 좋은 맛에 대한 기호도는 Aspergillus sp. SH-607 시료와 Aspergillus sp. SH-412, Rhizopus sp. SH-606, Aspergillus sp. SH-613, Rhizopus sp. SH-654, Aspergillus sp. SH-696 Aspergillus kawachii 시료가 유의성 있게 좋은 맛을 나타내 기호도가 높았으며 맛과 기호도가 가장 좋았던 것은 Aspergillus sp. SH-607 시료로 나타났다.

• Journal of the Korean Geophysical Society
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• v.8 no.2
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• pp.81-87
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• 2005

Acquisition and processing of vector seismic waves were conducted through simultaneous generation of P, SH, and SV waves and receiving those waves using three-component geophones. Test data were received by 24 8-Hz geophones at an interval of 2 m along a 94-m profile. The data were recorded for 512 ms with sampling intervals of 0.2 ms. Raw data indicate that both reflected and refracted P waves are strongly recorded on the vertical component while SH waves are significant on the transverse horizontal component. On the inline horizontal component, both direct P and converted PS waves are recorded. First arrivals of P and SH waves were detected simultaneously on the vertical and transverse horizontal axes, respectively. The recorded vector data were separately inverted using traveltime tomography to yield P- and SH-wave sections. Using those two velocity sections, Poisson's ratios were able to be obtained effectively.

• Korean Journal of Plant Tissue Culture
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• v.26 no.3
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• pp.219-222
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• 1999

To induce adventitious buds, hypocotyl and cotyledonary explants from 7 to 10 day-old seedlings of lettuce (Lactuca sativa L.: two Japanese cultivars of crisphead lettuce and four Korean cultivars of leaf lettuce) were cultured or Murashige and Skoog (MS) and Schenk and Hildebrandt (SH) media supplemented with BA and NAA in the light for five weeks. Cotyledonary explants produced adventitious shoots at greater frequencies than hypocotyl explants. MS medium was more favorable to adventitious shoot formation than HS medium. Combination of 0.5 mg/L BA and 0.1 to 1 mg/L HPh in MS medium led to the greatest frequency (86%) in adventitious shoot formation. Creator than 95% of shoots excised from explants were rooted when cultured on MS basal medium.

• Journal of The Korean Society of Grassland and Forage Science
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• v.20 no.1
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• pp.25-30
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• 2000

The conditions for callus formation and plant regeneration were confirmed in Italian ryegrass (Lolium mulfiorum Lam.). Among SH (Schenk and Hildebrandt), MS (Murashige and Skoog) and N6 medium (Chu) MS medium was highest degree of efficiencies respectively in callus formation and plant regeneration. In this study, we determined volume of hormones and other compounds appended in media. For callus formation, only $5\;mg/\;{\ell}$ of 2,4-D (2,4-dichlorophenoxy acetic acid) was appended in their media. For plant regeneration, we used MS medium containing $1.0\;mg/\;{\ell}$ of BA and $0.1\;mg/\;{\ell}$ of NAA.

• Proceedings of the Korean Society of Grassland Science Conference
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• 1999.06a
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• pp.93-93
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• 1999

4품종의 알팔파 (Medicago sativa L.) 종자로부터 직접 캘러스를 유도하고, 형성된 캘러스로부터 식물체를 재분화하는 조건을 확립하였다. 공시품종중 "Vernal" 품종이 캘러스 유도 및 재분화에서 가장 우수한 것으로 판명되었으며, SH(Schenk and Hildebrandt), MS (Murashige and Skoog), N6 (Chu) 배지중에서 SH 배지가 캘러스 유도와 재분화시에 모두 유리한 것으로 나타났다. 캘러스 유도 및 증식시에는 2,4-D를 3mg/$\ell$ 농도로 첨가한 SH-3 배지에서 가장 효율이 좋았으며, kinetin을 첨가하거나 kinetin의 농도가 높을수록 캘러스 형성 정도는 급격히 저하되었다.(중략)저하되었다.(중략)

• Journal of Korean Society of Forest Science
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• v.98 no.6
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• pp.719-725
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• 2009

This study was conducted to establish the optimal condition for acclimatization from somatic embryos of Eleutherococcus seoulensis. Torpedo-shaped embryos of Eleutherococcus seoulensis were cultured on 1/3 MS and WPM media supplemented with $GA_3$ (3.0, 5.0 mg/L) for 4 weeks. Plentlets were transferred to 1/2 SH solid medium with 1.0 mg/L $GA_3$ and 0.2% activated charcoal for shoot and root elongation and them elongated plantlets further developed on 1/2 SH medium for 4 weeks. Developed plantlets further elongated into well-shaped leaf and root system on 1/3 SH medium under ventilation condition for 4 weeks. Plantlets grew normally on 1/3 SH basal medium, were acclimated on various soil. Survival frequency of plantlets was influenced by soil type(peatmoss+perlite, perlite, soil on Nam mountain). The highest survival rate to soil was more than 70% when plantlets were 1/3 SH medium under ventilation condition in Nam mountain soil. These results indicate that the systematic procedure of plant production in Eleutherococcus seoulensis could be practically applicable for mass propagation.

• Journal of The Korean Society of Grassland and Forage Science
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• v.18 no.3
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• pp.267-272
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• 1998

We confirmed conditions for callus formation and plant regeneration of five varieties of orchardgrass (Dactylis glomerma L.). Among five varieties of orchardgrass, "Hapsung 19" expressed the highest rate for both of callus formation and plant regeneration. Otherwise, among SH (Schenk and Hildebrandt), MS (Murashige and Skoog) and N6 medium (Chu), MS and N6 medium were highest degree of efficiencies in callus formation and plant regeneration, respectively. In this study, we determined volume of hormones appended in media; $3\;mg/\;{\ell}$ ofdicamba for callus formation and $1\;mg/\;{\ell}$ of NAA (I-naphtalene acetic acid) and $5\;mg/\;{\ell}$ kinetin (6-furfurylaminopurine) for plant regeneration were appended in their media. We obtained orchardgrass plants from callus about 50~80 days after transferred to regeneration media.ation media.

• The Journal of Korean Medicine
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• v.43 no.3
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• pp.79-93
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• 2022

Objectives: We investigated the isoflavone contained in SH003 and its fractions, and the effect of these components on the inhibition of breast cancer. Methods: The isoflavones in solvent fractions of SH003 extract were identified by UPLC-MS and its contents were quantified using HPLC analysis. The estrogenic activity of SH003 or fractions was assessed by ERE luciferase assay in estrogen receptor (ER)-positive MCF-7 cells. To test the breast cancer inhibitory effect, the cell viability was measured using an MTT assay. Results: In this study, we demonstrated that SH003 and fractions contain 4 isoflavones which are calycosin-7-β-D-glucoside, formononetin-7-β-D-glucoside, calycosin, and formononetin. Despite containing isoflavones, estrogen-dependent transcription activity was not altered by both SH003 and fractions. On the other hand, SH003 and fractions inhibited the cell viability of breast cancer. In addition, its isoflavone components also showed reduced cell viability in various breast cancer cells. Conclusions: Overall, the phytoestrogen included in SH003 and fractions did not influence the estrogenic activity, emphasizing the safety of SH003 and fractions in breast cancer treatment.