• ALGAE
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• 제36권2호
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• pp.111-121
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• 2021

Two cyanobacterial morphotypes isolated from Signy Island, South Orkney Islands, maritime Antarctica were characterised using a polyphasic approach combining morphological, cytological and molecular analyses. These analyses showed that the strains grouped with members of the genus Wilmottia. This genus currently includes three species, W. murrayi, W. stricta, and W. koreana. Both morphotypes analysed in this study were placed within the clade of W. murrayi. This clade showed a well-supported separation from Antarctic and New Zealand strains, as well as strains from other regions. W. murrayi was first described from Antarctica and is now known from several Antarctic regions. Confirmation of the occurrence of W. murrayi at Signy Island significantly extends its known distribution in Antarctica. In addition, a new combination, W. arthurensis, is suggested for Phormidium arthurensis.

• 한국해양생명과학회지
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• 제1권1호
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• pp.8-17
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• 2016

톱상어과는 톱상어목에 속하며 지금까지 2속 8종이 보고되었다. 우리나라에 서식하는 톱상어과 어류는 톱상어 1종으로 알려져 있다. 톱상어과는 주둥이 양쪽에 작고 큰 이빨이 나있으며 납작하고 긴 것이 특징이다. 톱상어의 집단구조를 알기 위해서 4개의 지역(한국, 개체수=6; 미야기현, 개체수=1; 고치현, 개체수=1; 오키나와, 개체수=8)에서 채집된 16개체를 대상으로 형태와 분자분석을 실시하였다. 형태분석결과 3가지의 유형으로 구분되었으며, 그 중 톱상어 A 유형은 한국과 일본에 서식하며 짧은 주둥이(전장의 26.8%), 폭넓은 주둥이(주둥이 길이는 주둥이 폭의 4.5배)를 가지며, 톱상어 모식표본과 유사한 점에서 톱상어(Pristiophorus japonicus)로 추정된다. 톱상어 B 유형은 오키나와에 서식하며 긴 주둥이(전장의 31.7%), 폭넓은 주둥이(5.2배)를 가져 Pristiophorus sp.1로 제안한다. 톱상어 C 유형은 오키나와에 서식하며 긴 주둥이(전장의 31.7%), 폭좁은 주둥이(6.3배)를 가져 Pristiophorus sp. 2로 제안한다. 나아가 톱상어 A 유형과 C 유형의 mtDNA cytb 영역을 비교한 결과 2.1~2.7% 차이를 보여 형태결과를 지지해 주었다. 향후 Pristiophorus sp. 1 및 Pristiophorus sp. 2의 분류학적 위치를 구명하기 위한 추가 연구가 필요하다.

• Journal of Periodontal and Implant Science
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• 제13권1호
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• pp.63.1-63.1
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• 1983

• 한국양봉학회지
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• 제33권4호
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• pp.227-237
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• 2018

The majority parasitic bee mites of Thailand in genus Tropilaelaps are infesting colonies of native bees (Apis dorsata) and introduced bees (A. mellifera). The investigation aims to study morphological and genetic variation of Tropilaelaps mites infected different hosts. Adult mites were collected from honey bee brood throughout Thailand. Traditional and geometrical morphometrics were measured on photograph by using TPS program. Additional, COI gene variations were examined by PCR-RFLP and nucleotides sequencing. Tree of mites relationships were constructed by NJ and MP assumptions. Morphometric results indicated T. mercedesae were major species infesting on A. dorsata and A. mellifera. Mophological variation represented at anal and epigynial plate, which the shape of the anal plate apex margin has been key character to identify between T. mercedesae (bell to blunt shape) and T. koenigerum (pear shape). However, the discriminant analysis suggested that geometric results were potential to classify Thai Tropilaelaps populations from different hosts better than traditional morphometric. Otherwise, PCR-RFLP clearly detected the site of Dra I and Xba I digestion of Thai Tropilaelaps morphotypes. The COI sequences of T. koenigerum were founded infesting only A. dorsata in Thailand and four sequences that related to the Thai T. mercedesae morphotypes. The NJ and MP tree were clearly classified Thai Tropilaelaps species which were suggested both from morphological and molecular analysis. This information might be basically of taxonomic status, but this should have implication for controlling these mites in Thailand and other countries.

• 생태와환경
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• 제34권3호통권95호
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• pp.185-191
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• 2001

Euglena viridis의 형태변이, 밀도 및 환경요인과의 관계를 규명하기 위하여, 무심천, 미호천, 대전천, 전주천, 광주천 및 금호강의 도시하천에서 1996년 12월 25일에서 1997년 1월 4일 사이에 E. viridis의 형태와 밀도 및 수환경 요인들을 조사하였다. 각 하천에서 Euglena viridis는 형태적으로 두 가지 형태형으로 구분되었으나, 엽록체가 별 모양인 점에서 동일종으로 동정되었다. 형태형 I은 E. viridis의 일반적인 형태와 일치하였고, 대부분의 정점에서 대발생하여 광주천에서 최대 $5386\;cells\;{\cdot}\;mL^{-1}$이었다. 형태형 I의 밀도는 암모늄과 아질산염에 각각 정의 상관관계 (r = 0.80과 0.68),질산염과는 부의 상관관계에 있었다. 형태형 II는 엽록체들의 가장자리가 거치형이며, 세포질 소립들이 불규칙하게 분산되어 있는 점 등이 특징이었다. 형태형 II의 밀도는 질산염과 정의 상관관계(r = 0.98)를 보인 반면, 암모늄이나 아질산염과는 각각 부의 상관관계에 있었다. 그러나 두 가지 형태형은 각각 인산염, 수온 및 pH는 밀도와 유의한 상관을 보이지 않았다. 이 결과는 E. viridis의 두 가지 형태형들이 서로 다른 질소성 영양염들을 이용하므로서 동 시기 및 동일 지소에서 공존하고 있다는 것을 보여준다.

• Journal of Periodontal and Implant Science
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• 제33권2호
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• pp.247-257
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• 2003

The purpose of this study was to evaluate the difference of subgingival bacterial compositions between periodontally healthy and diseased sites. Subgingival plaque samples were obtained from 100 sites in 20 untreated adult periodontitis patients(experimental group), and 100 sites in healthy individuals(contro1 group). Before sampling, probing pocket depth(PPD) and clinical level of attachment(CAL), Plaque Index(PI), and Sulcus Bleeding Index(SBI) were recorded for each sampled sites. Microbial samples were collected from the bases of gingival sulci or periodontal pockets with sterile curettes. The samples were examined under darkfield microscope(${\times}$400). At least 150 bacteria were evaluated and categorized on the basis of bacterial morphology and motility, i.e. cocci, non-motile rods, motile rods, and spirochetes. In control group, subgingival microbial flora consisted of 73.7% of cocci, 20.0% of non-motile rods, 4.3% of motile rods, and 2.0% of spirochetes. The microbial samples from experimental group consisted of 51.5% of cocci, 19.4% of non-motile rods, 17.6% of motile rods, and 11.6% of spirochetes. The proportion of cocci was higher in control group than in experimental group. Proportions of motile rods and spirochetes were higher in experimental group than in control group. The proportion of nonmotile rods in experimental group and control group was not significantly different. Sulcus Bleeding Index and Plaque Index showed high correlation with the bacterial composition. These findings suggests that examination of subgingival bacterial proportion may serve as more sensitive mirror of the local periodontal status than clinical parameters.

• Restorative Dentistry and Endodontics
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• 제47권2호
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• pp.19.1-19.15
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• 2022

Objectives: The aim of this study was to evaluate and compare the apical constriction (AC) and apical canal morphology of maxillary first and second molars, using micro-computed tomography (micro-CT). Materials and Methods: The anatomical features of 313 root canals from 41 maxillary first molars and 57 maxillary second molars of patients with known age and sex were evaluated using micro-CT, with a resolution of 26.7 ㎛. The factors evaluated were the presence or absence of AC, the morphotypes, bucco-lingual dimension, mesio-distal dimension, and the profile (shape) of AC and the apical root canal. The apical root canal dimensions, location of the apical foramen (AF), AC to AF distance, and presence of accessory canals in the apical 5 mm were also assessed. Descriptive and analytical statistics were used for data evaluation. Results: AC was present in all 313 root canals. Patients' age and sex did not significantly impact either AC or the apical canal dimensions. The most common AC morphotype detected was the traditional (single) constriction (52%), followed by the parallel (29%) morphotype. The mean AC dimensions in maxillary first molars were not significantly different from those in maxillary second molars. Sixty percent of AF were located within 0.5 mm from the anatomic apex. Conclusions: The most common morphotype of AC detected was the traditional constriction. Neither patients' age nor sex had a significant impact on the dimensions of the AC or the apical root canal. The majority of AF (60%) were located within 0.5 mm from the anatomic apex.

• Fisheries and Aquatic Sciences
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• 제4권3호
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• pp.120-129
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• 2001

Thirty-six dinoflagellate cysts, representing 15 genera were identified in the surface sediments obtained from the northwestern East China Sea. Three cyst morphotypes found in this survey have not previously been described in the East China Sea and adjacent waters: Seleno­pemphix sp. 2, Selenopemphix sp. 3 and Trinovantedinium sp. 1. In the northwestern East China Sea, Operculodinium centrocarpum, Spiniferites bulloideus and ellipsoidal cysts of Alexandrium were commonly observed. Moreover, it was recognized that the ellipsoidal cysts of Alexandrium, whose motile cells of A tamarense and/or A catenella are responsible to paralytic shellfish poisoning, distributed not only restricted to the coastal areas but also to the offshore stations far from the Changjiang River mouth.

• ALGAE
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• 제21권4호
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• pp.349-375
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• 2006

The application of modern ecological, ultrastructural and molecular methods, aided by the cultivation of numerous cyanobacterial morphotypes, has substantially changed our knowledge of these organisms. It has led to major advances in cyanobacterial taxonomy and criteria for their phylogenetic classification. Molecular data provide basic criteria for cyanobacterial taxonomy; however, a correct phylogenetic system cannot be constructed without combining genetic data with knowledge from the previous 150 years research of cyanobacterial diversity. Thus, studies of morphological variation in nature, and modern morphological, ultrastructural, ecophysiological and biochemical characters need to be combined in a “polyphasic” approach. Taxonomic concepts for generic and infrageneric ranks are re-evaluated in light of combined phenotypic and molecular criteria. Despite their usefulness in experimental studies, the limitations of using strains from culture collections for systematic and nomenclatural purposes is highlighted. The need for a continual revision of strain identification and proper nomenclatural practice associated with either the bacteriological or botanical codes is emphasized. Recent advances in taxonomy are highlighted in the context of prospects for understanding cyanobacterial diversity from natural habitats, and the evolutionary and adaptational processes that cyanobacteria undergo.

• Journal of Plant Biology
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• 제35권1호
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• pp.61-67
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• 1992

환경과 관련된 해산 녹조류 잎파래(Enteromorpha linza)의 형태적 변이 양상을 파악하기 위하여 한국 전 해안에서 채집된 재료를 대상으로 각 형질을 정량화한 다음 주성분 분석을 실시하였다. 그 결과 파도 등에 노출된 개체군의 식물체는 보호된 개체군의 식물체보다 자루의 길이와 엽록체의 최대 길이가 길고 엽체의 폭은 좁은 형태적 특징을 보였다. 각 형질에 대한 개체군 간의 형태적 차이 유무를 보여주는 다중비교분석에서도 이와 같은 결과를 확인할 수 있어, 파도 등에 의한 노출 정도로 인하여 나타나는 표현형의 변이성 출현에 대한 타당성을 확인할 수 있었다.