Journal of Periodontal and Implant Science

Korean Academy of Periodontology (대한치주과학회)
권호 표지

Darkfield microscopic study of the bacterial morphotypes in the periodontal pockets of Korean adult periodontitis patients

한국 성인형 치주염 환자의 치주낭 내 세균분포에 관한 암시야현미경적 연구

  • Park, Jung-Min (Department of Periodontology, College of Dentistry, Kangnung National University) ;
  • Nam, Ki-Yoon (Department of Periodontology, College of Dentistry, Kangnung National University) ;
  • Lee, In-Kyeong (Department of Periodontology, College Of Dentistry, Seoul National University) ;
  • Um, Heung-Sik (Department of Periodontology, College of Dentistry, Kangnung National University) ;
  • Chang, Beom-Seok (Department of Periodontology, College of Dentistry, Kangnung National University)
  • 박정민 (강릉대학교 치과대학 치주과학교실) ;
  • 남기윤 (강릉대학교 치과대학 치주과학교실) ;
  • 이인경 (서울대학교 치과대학 치주과학교실) ;
  • 엄흥식 (강릉대학교 치과대학 치주과학교실) ;
  • 장범석 (강릉대학교 치과대학 치주과학교실)
  • Published : 2003.06.30
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Abstract

The purpose of this study was to evaluate the difference of subgingival bacterial compositions between periodontally healthy and diseased sites. Subgingival plaque samples were obtained from 100 sites in 20 untreated adult periodontitis patients(experimental group), and 100 sites in healthy individuals(contro1 group). Before sampling, probing pocket depth(PPD) and clinical level of attachment(CAL), Plaque Index(PI), and Sulcus Bleeding Index(SBI) were recorded for each sampled sites. Microbial samples were collected from the bases of gingival sulci or periodontal pockets with sterile curettes. The samples were examined under darkfield microscope(${\times}$400). At least 150 bacteria were evaluated and categorized on the basis of bacterial morphology and motility, i.e. cocci, non-motile rods, motile rods, and spirochetes. In control group, subgingival microbial flora consisted of 73.7% of cocci, 20.0% of non-motile rods, 4.3% of motile rods, and 2.0% of spirochetes. The microbial samples from experimental group consisted of 51.5% of cocci, 19.4% of non-motile rods, 17.6% of motile rods, and 11.6% of spirochetes. The proportion of cocci was higher in control group than in experimental group. Proportions of motile rods and spirochetes were higher in experimental group than in control group. The proportion of nonmotile rods in experimental group and control group was not significantly different. Sulcus Bleeding Index and Plaque Index showed high correlation with the bacterial composition. These findings suggests that examination of subgingival bacterial proportion may serve as more sensitive mirror of the local periodontal status than clinical parameters.

Keywords

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