• 대한한의학회지
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• 제29권1호
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• pp.167-178
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• 2008

Object : Gliosis becomes a physical and mechanical barrier to axonal regeneration. Reactive gliosis induced by hypoxic brain injury is involved with up-regulation of CD81 and GFAP. The current study was to examine the effect of the Angelica Sinens on CD81 and GFAP regulation after hypoxic brain injury in the astrocyte. Methods : MTT assay was performed to examine cell viability, and cell based ELISA, western blot and PCR were used to detect the expression of CD81 and GFAP. Results : The following results were obtained: 1. Using ELISA, western blot and PCR from the astrocyte after hypoxic injury, CD81 and GFAP expression was seen to have increased. 2. After the administration of Angelica Sinens extract to astrocyte following hypoxic injury, CD81 and GFAP expression was down regulated significantly. The water extract of Angelica Sinens prevented cell destruction by hypoxic induced with $CoCl_2$. Conclusion : These results indicate that Angelica Sinens could suppress reactive gliosis, which disturbs astrocyte regeneration after hypoxic brain injury by controlling the expression of CD81 and GFAP.

• The Plant Pathology Journal
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• 제33권6호
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• pp.608-613
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• 2017

The full-length sequence of a new isolate of Apple chlorotic leaf spot virus (ACLSV) from Korea was divergent, but most closely related to the Japanese isolate A4, at 84% nucleotide identity. The full-length cDNA of the Korean isolate of ACLSV was cloned into a binary vector downstream of the bacteriophage T7 RNA promoter and the Cauliflower mosaic virus 35S promoter. Chenopodium quinoa was successfully infected using in vitro transcripts synthesized using the T7 promoter, detected at 20 days post inoculation (dpi), but did not produce obvious symptoms. Nicotiana occidentalis and C. quinoa were inoculated through agroinfiltration. At 32 dpi the infection rate was evaluated; no C. quinoa plants were infected by agroinfiltration, but infection of N. occidentalis was obtained.

• 대한원격탐사학회지
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• 제39권6_1호
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• pp.1225-1234
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• 2023

작물의 수분 스트레스는 생육 단계와 온도 조건에 따라 달라질 수 있으며, 이는 생육에 부정적이고 생산량에 피해를 줄 수 있다. 원격탐사 기법을 이용한 보다 정확한 스트레스 탐지를 위해서는 수분 결핍의 가뭄뿐만 아니라 폭염 조건도 함께 고려할 필요가 있으며, 작물 반응을 민감히 표현할 식생지수를 선정하고 그 변화 값을 이해해야 한다. 본 연구는 콩을 대상으로 영양생장기와 생식생장기로 구분해 가뭄과 폭염 조건에서의 MERIS terrestrial chlorophyll index (MTCI)와 chlorophyll/carotenoid index (CCI)를 측정하였다. 엽록소 농도에 민감한 MTCI는 가뭄에 민감하게 감소했으며, 폭염이 동시에 발생했을 때는 그 정도가 컸다. 광합성능과 상관이 있는 CCI는 비교적 가뭄에 민감하지 않으나, 폭염을 동반하면 큰 폭으로 감소했다. 수분 결핍 회복 후 MTCI는 피해 전보다 값이 증가했으며, CCI는 열 스트레스에 민감해졌다. 이러한 결과는 원격탐사를 활용해 콩의 수분 스트레스를 평가하고 관수 일정을 조정하는 데에 기여할 것으로 기대된다.

• Clinical and Experimental Reproductive Medicine
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• 제22권2호
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• pp.171-181
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• 1995

Ovarian hyperstimulation syndrome(OHSS) is one of the well-known complication of controlled ovarian hyperstimulation(COH). Though there have been numerous measures to prevent the occurrence of OHS, it has not been completely preventable until now. The fluid shift from the intravascular space to the third space is due to decreased oncotic pressure of the serum. The objective of this study was to evaluate if IV administration of 20% albumin in those patients with OHSS risk can make prevention of severe OHSS. We retrospectively analysed 70 patients undergoing IVF-ET who had serum peak estradiol($E_2$) level of >2,500 pg/ml and/or the number of oocytes retrieved over 20. The treatment group(n=39) received albumin while the control group(n=31) did not. After 40 grams of human albumin diluted in 1,000 ml of 0.9% sodium chloride solution, the treatment group received half of the fluid during oocyte retrieval, the remainder in the recovery suite. The results were as follows; There were significant differences in the levels of serum peak $E_2$ and number of oocytes retrieved between the two groups(p<0.05). However, there were no significant differences in the incidence of OHSS and pregnancy rate or multifetal pregnancy rate. In conclusion, administration of albumin to OHSS risk patients did not reduce the rate of OHSS in IVF-ET. However, if we consider the fact that there were differences in the level of peak serum $E_2$ and oocyte numbers, further prospective study may be needed.

• 유통과학연구
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• 제14권1호
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• pp.75-84
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• 2016

Purpose - In accordance with the recent changes in the management environment, leaders are required to have an ability of drawing and inducing potential of each worker. Coaching, as one of the strategies of human resources development, is drawing people's attention. This study aims to inquire about the mechanism of leader's coaching behavior which influences the attitude and behavior of workers. Firstly, this study intends to illustrate what kind of influences the leader's coaching behavior can give on organizational citizenship behavior and turnover intention of workers. Secondly, it plans to verify the mediating effect of authentic leadership in the relationship between leader's coaching behavior and organizational citizenship behavior and turnover intention of workers. Thirdly, it aims to prove the moderating effect of emotional intelligence in the relationship among leader's coaching behavior, organizational citizenship behavior and turnover intention of workers. Research design, data and methodology - In order to figure out how the leader's coaching behavior influences attitude of workers, authentic leadership is considered as mediating effect. As for a moderating effect, we have determined emotional intelligence which attracts attention in the recent studies on organizational behavior, so as to investigate the correlation among variables. As for study sample, it was targeted to 236 organizational members of distribution corporations whose leader's coaching behavior is required. For reaching the purpose of study, SPSS 20 was applied for the analysis. Results - The research findings can be summarized as follows: Firstly, leader's coaching behavior gives significant positive influences on organizational citizenship behavior. Moreover, leader's coaching behavior gives negative influences on turnover intention of organizational members. Secondly, the mediating effect of authentic leadership was proved in the relationship between leader's coaching behavior and attitude of workers. Based on the analysis, it turned out that authentic leadership doesn't give a mediating effect in the bilateral relationship. Lastly, the moderating effect of emotional intelligence was proved in terms of how the leader's coaching behavior influences the attitude of workers. The research result shows that the emotional intelligence influences towards the negative direction, in the correlation between coaching behavior and organizational citizenship behavior On the other hand, in the relationship with turnover intention, It was significant effect. Conclusions - Based on the research findings, we have intended to inquired about the mechanism of leader's coaching behavior which influences the attitude and behavior of workers. Furthermore, in the operational level, the following implications can be given: the importance of coaching activation in the operational level was confirmed, through inquiring into the influence of leader's coaching behavior on workers. Moreover, the importance of emotional intelligence, which has been steadily raised, was also confirmed. It is necessary for future study to carry out additional research on various factors of coaching which can influence the effectiveness of coaching, for instance, coaching process, coach's competency, characteristics of coaching customers, relationship with coaching customers and coaching system.

• 보건행정학회지
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• 제30권4호
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• pp.438-443
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• 2020

The digital treatment technology industry is one of the core fostering industries of the Moon Jae-in government along with the global trend. The purpose of this study is to compare and analyze the investment and economic ripple effect on the related industries. To this end, we used the industry-related table, which is the actual measurement data for 2015 that the Bank of Korea actually measured and released every 5 years in 2019. The digital treatment technology industry was not clearly classified within Korea's industrial classification system, so the contents of the industry-related survey were analyzed, and the digital treatment technology industry was reclassified and then analyzed. As a result of the analysis, it was analyzed that the production induction effect of the digital treatment technology-related industry in 2015 was 1.770, the value-added induction effect was 0.875, and the employment induction effect was 19.128, which was higher than that of other industries in Korea. As a result of the analysis of the economic ripple effect (scenario 1), the production inducing effect was about 370 billion won, the added value inducing effect was about 185 billion won, and the employment inducing effect was 4,044 people. The results of this study are expected to play a large role in economic revitalization as the effect of inducing production, increasing employment, and creating added value through fostering the digital treatment technology industry is expected to play a large role in activating the economy. It is expected to play a large role in providing central medical services. Therefore, it is expected that policy support for revitalizing the digital treatment technology industry through active investment support and tax benefits from the government to foster the digital treatment technology industry is necessary.

• 한국식품위생안전성학회지
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• 제29권4호
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• pp.285-291
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• 2014

농산물 중에 있는 제초제 saflufenacil의 잔류량을 검사하기 위해 HPLC-UVD와 LC-MS를 이용한 정확하고 감도가 좋은 분석방법을 개발하였다. Saflufenacil 잔류물은 acetone 추출, dichloromethane을 이용한 액-액 분배, silica와 carbon 카트리지 정제를 거쳐 기기분석을 수행하였다. 검량선 작성을 위해 $0.1{\sim}5.0{\mu}gmL^{-1}$ 범위로 표준품을 만들어 실험한 결과 상관계수($r^2$)는 0.999로 높은 직선성을 보였다. $0.02{\sim}0.5mgkg^{-1}$ 처리수준으로 회수율을 실험한 결과는 80.5~110.2% 이었으며, 상대표준편차는 10% 미만이었다. 분석방법의 검출한계와 정량한계는 각각 0.005와 $0.02mgL^{-1}$ 이었다. 확립된 시험법으로 본청, 부산지방식품의 약품안전청과 경인지방식품의약품안전청에서 실험실간 검증을 실시한 결과 만족스런 결과를 얻었다. 이러한 결과들을 통해 확립된 시험법은 농산물 중 saflufenacil의 잔류량 분석에 적합함을 확인할 수 있었다.

• 벤처창업연구
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• 제16권3호
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• pp.43-58
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• 2021

본 연구는 역대 정부 창업정책을 Hall(1993)의 정책 패러다임(Policy Paradigm) 관점에서 분석하였다. 총 195건의 신문기사와 202건의 정부 문서를 살펴보고, 각 정부 별 정책목표, 정책수단, 그리고 정책수단의 수준변화 분석을 통해 정책 패러다임 변동을 확인하였다. 첫 번째 패러다임 구축 시기는 제5·6공화국으로 1986년 「중소기업창업 지원법」 제정과 함께 제조업 중심의 창업 촉진 및 성장·발전 지원이라는 목표 설정으로 창업정책 패러다임이 처음 구축된 시기이다. 다음은 국민의 정부시기로 IMF 외환위기 대응을 위한 「벤처기업육성에 관한 특별조치법」제정으로 벤처기업 창업 촉진과 육성이라는 새로운 정책 목표를 설정하고, 이를 달성하기 위한 수단을 강구하였다. 마지막 패러다임 변화는 4차 산업혁명과 코로나19에 적절히 대응하기 위해 문재인 정부시기에 이루어졌다. 「중소기업창업 지원법」전부개정을 통해 창업 개념 확장, 신산업·기술창업 지원 및 집중 육성을 도모하였으며, 벤처투자관련 법을 일원화하고 정비하였다. 또한 중소기업청을 중소벤처기업부로 승격시켜, 창업정책을 주도적으로 실행할 수 있도록 하였다. 특히, 본 연구는 최근 창업정책 이슈인 창업기업의 낮은 생존율과 민간투자 활성화에 대하여 살펴보고, 새로운 창업정책 패러다임 변동의 필요성을 제시하였다.

• 대한원격탐사학회지
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• 제40권3호
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• pp.275-284
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• 2024

농업 분야에서 농작물의 생장 변화와 수분량 파악은 매우 중요하다. 본 연구에서는 수분에 민감하게 반응하는 단파적외(Short Wavelength Infrared, SWIR) 센서를 드론에 탑재하여 콩의 생장과 수분량 변화를 관찰하였다. 콩의 생장이 활발해질 때 수분량이 증가하는 현상을 확인했으며, 관수량의 차이에 따라 식생지수와 수분지수가 다르게 감소하는 것을 확인했다. 이는 습해로 인해 콩의 생장 둔화와 수분량 감소가 나타났음을 시사한다. 본 연구는 농작물의 생산성 저하와 습해 피해 감소를 위해 콩의 생장 단계별 식생지수와 수분지수를 세밀하게 모니터링할 것을 제안한다. 이로써 습해 뿐만 아니라 가뭄과 같은 수분 변화가 농작물 생장에 미치는 악영향을 조기에 탐지하여 예방할 수 있을 것으로 판단한다. 드론 기반 SWIR 센서를 통한 수분 스트레스의 조기 진단 가능성을 제시함으로써 대규모 농작물 관리의 효율성을 높이고 수확량을 증가시켜 농업 생산에 기여할 것으로 기대된다.

• 생약학회지
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• 제46권3호
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• pp.214-222
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• 2015

Recently, many studies have focused on the aging and oxidative stress. Several papers reported that Vigna angularis has various biological properties including antiaging, antioxidative and anti-inflammatory activities. Methanol extract from the red bean sprouts was successively partitioned as n-hexane, methylene chloride, ethyl acetate, n-butanol and H₂O soluble fractions. We had studied lifespan extending and stress resistant effects of the fractions using Caenorhabditis elegans. Superoxide dismutase (SOD), catalase activities, and intracellular reactive oxygen species (ROS) levels were also investigated. Moreover, we had studied to find any significant change in aging-related factors such as reproduction, food intake, growth and movement of C. elegans. Our results represent that ethyl acetate fraction showed the most potent lifespan extending and stress resistant effects, and this fraction was able to elevate SOD and catalase activities of worms, and reduce intracellular ROS accumulation.