• Korean Journal of Microbiology
• /
• v.36 no.3
• /
• pp.181-186
• /
• 2000

The cellulosome of Clo.~tr~rlil~m tl\ulcornererfnocellum consistmg of 26 dfferent polypeptides contains calcium. The polypeptides dissociated when calcium was removed. Most of dockerill region in the catalytic polypeptides cleavcd during dmociation. The dissociated polypeptides were well separated by MonoQ column chromatography into CipA containing fraction, a fraction still complexed wit11 91 kDa (CelK-a). 60 IiDa and 57 kDa polypeptides, and fractious contailling mainly single polypeptide of 46 kDa (CelA-a) or 71 1d)a polypeptide (CelS-trj Most or the fractions hydrolyzed c~ystalliue cellulose The purified 71 kDa polypeptide was strictly dependent on calcium for crystalline cellulose hydvolyzing activities a1 $60^{\circ}C$~$70^{\circ}C$ but 46 kDa polypeptide was not. 46 M)a polypeptide digested cellodextri~~ as cellobiose or cellotriose unit, and glucose was produced together with cellobiose and cellotriose froln cellotetraosc. It seems that cellulosome produces final product, cellobiose, through coordinated ~qulation of activities of vannus subunits.

• Journal of Mushroom
• /
• v.21 no.4
• /
• pp.209-214
• /
• 2023

In this study, Pleurotus ostreatus No.42 was cultured in glucose-peptone-yeast-wheat bran medium using a previously reported novel rotary draft tube bioreactor. Versatile peroxidase (VP), a lignin-degrading enzyme, was isolated from a pellet-type mycelium culture grown in the medium for seven days. The VP was purified by sequentially applying ultra-filtration, DEAE-Sepharose CL-6B column, and Mono Q column. SDS-PAGE analysis revealed the molecular weight of VP to be 36.4 KDa with an isoelectric point of 3.65. The amino acid sequence was confirmed as VTCATGQTT. The purified VP was observed to possess the property of not only oxidizing Mn ions but also decomposing veratryl alcohol, a non-phenolic compound. The catalytic ability of VP is a subject for future research.

• Korean Journal of Environment and Ecology
• /
• v.11 no.1
• /
• pp.111-125
• /
• 1997

To investigate the climax forest structure and to construct the ecological basic data, forty nine plots were set up and surveyed in Mt. Minjuji, Chungchongpukdo. According to the analysis of classification by TWINSPAN, the community was divided by seven groups of Pinus densiflora-Carpinus laxiflora-Quercus serrata(community I), Q. mongolica-Q. serrata-Platycarya strobilacea(community II), Q. mongolica(community III), Fraxinus mandshurica-Acer mono(community IV), Cornus controversa-F. mandshurica(community V), F. mandshurica-Carpinus cordata(community VI), and F. mandshurica-C. laxiflora(community VII). In the results of the analysis of species structure, similarity, diversity and DBH, except for community I~III, it was founede out broadleaves-mixed-climax forest. Constructed basic data will be applied to sustainable development such as ecotourism, nature trail etc.

• Korean Journal of Environment and Ecology
• /
• v.12 no.2
• /
• pp.119-130
• /
• 1998

The purposes of this study were to investigate vegetation structure and to present management plan of mountain type green space in Kangwhado, Inchon. The actual vegetation in survey sites(11,331ha) was divided into 19 community types. It was consisted of secondary forest(92.32%) which was Quercus acutissima, Pinus densiflora-Q. acutissima, and Q. mongolica community so on. Artificial planting forest area, such as Robinia pseudoacacia and Pinus rigida forest and others, was 5.40%(612ha) and it was less than cases in other cities in the Metropolitan area. According to the classification by TWINSPAN, 57 survey plots were divided into seven community types; P rigida(community A), Q. acutissima(community B) P. densiflora-Q. acutissima(community C), Q. acutissima-P. densiflora (community D), P. densiflora-Carpinus laxiflora-Q. serrata-Q. acutissima(community E), Q. serrata-Q. mongolica(community F), and Zelkova serrata-Acer mono(community G). From this result, ecological succession trend of vegetation in this area seems to be change from P. densiflora forest through Q. acutissima forest to Q. mongolica, Q. serrata, and C. laxiflora forest. It was similar to the ordinary successional trend of temperate deciduous forest in middle area, Korea. In addition, this study area was showed acid soil(pH 4.17). Therefore, there is a need for managing the soil environment for effective vegetation management.

• Korean Journal of Environment and Ecology
• /
• v.31 no.3
• /
• pp.297-317
• /
• 2017

The research has been conducted in order to classify the vegetation types of natural forest in Mt. Janggunbong, Bonghwa-Gun and establish its following characteristics of stands. Vegetation data were collected by June in 2014 to August in 2016. Based on the 71 quadrates, we carried out an analysis of species-area curve, vegetation types, importance value, species diversity, coincidence method and CCA. As a result of the species-area curve, it was showed the proper investigation, vascular plants consisted a total of 227 taxa; 67 families, 152 genera, 197 species, 3 subspecies, 27 varieties. The forest vegetation was classified into 7 vegetation units, when it comes to a community group, they are classified into the Quercus mongolica community group. As for community, Q. mongolica community group is classified into the Acer pictum subsp. mono community, Pinus koraiensis community and Q. variabilis community. As for group, A. pictum subsp. mono community is classified into Lychnis cognata group and Deutzia glabrata group. The P. koraiensis community is classified into A. komarovii group and P. koraiensis typical group. The Q. variabilis community is classified into Parthenocissus tricuspidata group and Q. variabilis typical group. As for subgroup, P. tricuspidata group is classified into P. tricuspidata typical subgroup and Salix caprea subgroup. As a result of the importance value, Q. mongolica showed highest importance value in tree layer of vegetation units 1, 2, 3, 4 and 5. Betula schmidtii showed highest importance value in tree layer of vegetation unit 6. P. densiflora showed highest importance value in tree layer of vegetation unit 7. In subtree layer, shrub layer and herb layer of vegetation units 6 and 7, Q. mongolica and Q. variabilis showed high importance value. This implying that a vegetation units 6 and 7 is predicted to be composed by Q. mongolica and Q. variabilis. As for species diversity, vegetation units 1, 2 and 3 showed high species diversity(2.130~2.353). It was considered to be due to the topographical characteristics of valley. As a result of coincidence method and CCA, altitude showed high correlation than other environmental factor.

• The Korean Journal of Pesticide Science
• /
• v.18 no.2
• /
• pp.104-114
• /
• 2014

Scale insects heavily damaged many agricultural crops and trees in urban and forest areas. This study was conducted to carry out alternative control methods for environmentally friendly control of scale insects. Hot water extract of Quisquali indica produced high mortality against the black pine bast scale, Matsucoccus thunbergianae in previously experiment. Methanol extract obtained from fruit of Q. indica was successively fractionated using hexane, chloroform, ethyl acetate, buthanol and water which were examined by exposing for their insecticidal activity against four Cocoidea species (Eriococcus lagerstroemiae, Ceroplastes japonicus, Crisicoccus pini and Planococcus citri) in laboratory. Hexane fraction gave the highest insecticidal activity against scale insect pest. GC-mass analysis confirmed that the main compounds of Q. indica were alpha-pinene, methyl palmitate, eugenol, methyl myristate, phthalic acid mono(2-ethylhexyl)ester and palmitic acid. Among those compounds, ${\alpha}$-pinene was included 10 hexane subfractions. $LC_{50}$ of ${\alpha}$-pinene was 0.0114 ppm against 2nd instar of E. lagerstroemiae. These results suggest that Q. indica extracts might be used as an alternative control agent of scale insects.

• Transactions of the Korean hydrogen and new energy society
• /
• v.19 no.2
• /
• pp.124-131
• /
• 2008

Crude cytoplasmic fraction of phototrophic purple sulfur bacterium, Thiocapsa roseopersicina NCIB 8347, were initially prepared and purified by sonication, ultracentrifugation, ammonium sulfate fractionation and heat-treatment and it has been previously reported. Using various applications of chromatography far the purification of membrane-bound and soluble hydrogenases from heat-treated enzyme fraction were studied at present report. When the heat-treated enzyme preparation was applied to the anion column chromatography using Q-sepharose, Fraction I and II, which were extracted with the KCl 0-0.5 M gradient, showed the specific evolution hydrogenase activity 3.86 and 2.27 U/mg-protein respectively. Specific hydrogenase activitys of Fraction I and II were further increased to 4.35 and 7.46 U/mg-protein for Fraction I and to 2.49 and 4.41 U/mg-protein fur Fraction II respectively, when hydrophobic interaction column, Phenyl superose, and anion exchange column, Mono-Q, were applied. Size exclusion chromatography using superdex 200 concentrated the hydrogenase Fraction I and II to 9.19 and 7.84 U/mg-protein respectively at the final step of purification.

• Fisheries and Aquatic Sciences
• /
• v.2 no.2
• /
• pp.218-225
• /
• 1999

A protease without tryptic and chymotryptic activities was purified from the hepatopancreas of shrimp, Penaeus orientalis, using Q-Sepharose ionic exchange, benzamidine Sepharose-6B affinity, Mono-Q, and gel chromatography. Molecular weight (M.W.) of the protease was estimated to be 27kDa by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS­PAGE). The amino acid composition of the protease was different from that of protease from P. japonicus or trypsin from P. orientalis. The protease was completely inhibited by benzamidine, $N\alpha-p-tosyl-L-lysine$ chloromethyl ketone (TLCK), and phenylmethylsulfonyl fluoride (PMSF) and was not affected by leupeptin, pepstatin, N-tosyl-L-phenylalanine chloromethyl ketone (TPCK), iodoacetate, and ethylenediamine tetra acetate (EDTA). The enzyme did not have any activity against Na-benzoyl-DL-arginine p-nitroanilide (BAPNA) or N-benzoyl-L-tyrosine ethyl ester (BTEE) which are specific substrates of trypsin and chymotrypsin, respectively. However, the protease showed hydrolytic activity for a carboxyl terminal of Tyr, Trp, Phe, Glu, and Cys.

• Food Science and Biotechnology
• /
• v.18 no.5
• /
• pp.1173-1179
• /
• 2009

Trypsin inhibitors and lectins are defense proteins produced by many organisms. From Chinese 'Large Black Soybeans', a 60 kDa lectin and a 20 Da trypsin inhibitor (TI) were isolated using chromatography on Q-Sepharose, Mono Q, and Superdex 75. The TI inhibited trypsin and chymotrypsin with an $IC_{50}$ of 5.7 and $5{\mu}M$, respectively. Trypsin inhibitory activity of the TI was stable from pH 3 to 13 and from 0 to $65^{\circ}C$. Hemagglutinating activity of the lectin was stable from pH 2 to 13 and from 0 to $65^{\circ}C$. The TI was inhibited by dithiothreitol, signifying the importance of disulfide bond. The TI and the lectin inhibited HIV-1 reverse transcriptase ($IC_{50}$=44 and $26{\mu}M$), and proliferation of breast cancer cells ($IC_{50}$=42 and $13.5{\mu}M$) and hepatoma cells ($IC_{50}$=96 and $175{\mu}M$). The hemagglutinating activity of the lectin was inhibited most potently by L-arabinose. Neither the lectin nor the TI displayed antifungal activity.

• Journal of Life Science
• /
• v.10 no.1
• /
• pp.20-23
• /
• 2000

Mono-ADP-ribosylation, catalyzed by ADP-ribosyltransferases, is a post-translational modification of proteins in which the ADP-ribose moiety of NAD is transferred to an acceptor protein. Previously, we have identified and cloned a glycosylphosphatidylinositol-linked ADP-ribosyltransferase (Yac-1) from mouse lymphoma cells. Yac-1 enzyme contains three regions (region I,II,III) similar to those found in several bacterial toxins and vertebrate ADP-ribosyltransferases. Site-directed mutagenesis was performed to verify the role of Glu 233 in region III. Mutants E233Q, E233D and E233A were inactive for ADP-ribosyltransferase activity. Thus Glu 233 in Yac-1 is essential for enzyme activity, suggesting that Glu 233 in Glu-rich motif near the carboxy terminus plays a catalytic role in ADP-ribosyltransferase activity.