• Journal of Microbiology and Biotechnology
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• v.11 no.6
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• pp.1087-1092
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• 2001

The effect of Sodium Butyrate (NaBu) on the N-linked oligosaccharide structure of Erythropoietin (EPO) was investigated. Recombinant human EPO was produced by CHO cells grown in an $MEM{\alpha}$ medium with or without 5 mM NaBu, and purified from the culture supernatants using a heparin-sepharose affinity column and immunoaffinity column. The N-linked oligosaccharides were released enzymatically and isolated by paper chromatography. The isolated oligosaccharides were then labeled with a fluorescent dye, 2-aminobenzamide, and analyzed with MonoQ anion exchange chromatography and GlycosepN amide chromatography for the assignment of a GU (glucose unit) vague. A glycan analysis by HPLC showed that the most significant characteristic effect of NaBu was a reduction in the proportion of glycans with Sri-and tetrasialylated oligodaccharides from $21.30\%$ (tri-) and $14.86\%$ (tetra-) in the control cultures (without NaBu) to $8.72\%$ (tri-) and $1.25\%$ (tetra-) in the NaBu-treated cultures, respectively. It was also found that the proportion of asialo-glycan increased from $12.54\%\;to\;23.6\%$ when treated with NaBu.

• Parasites, Hosts and Diseases
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• v.41 no.4
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• pp.189-196
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• 2003

In order to evaluate the possible roles of secretory proteases in the pathogenesis of amoebic keratitis, we purified and characterized a serine protease secreted by Acanthamoeba lugdunensis KA/E2, isolated from a Korean keratitis patient The ammonium sulfate-precipitated culture supernatant of the isolate was purified by sequential chromatography on CM-Sepharose, Sephacryl S-200, and mono Q-anion exchange column. The purified 33 kDa protease had a pH optimum of 8.5 and a temperature optimum of $55^{\circ}C$. Phenylmethylsulfonylfluoride and 4-(2-Aminoethyl)-benzenesulfonyl-fluoride, both serine protease specific inhibitors, inhibited almost completely the activity of the 33 kDa protease whereas other classes of inhibitors did not affect its activity. The 33 kDa enzyme degraded various extracellular matrix proteins and serum proteins. Our results strongly suggest that the 33 kDa serine protease secreted from this keratopathogenic Acanthamoeba play important roles in the pathogenesis of amoebic keratitis, such as in corneal tissue invasion, immune evasion and nutrient uptake.

• Proceedings of the Korean Environmental Sciences Society Conference
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• 2001.05a
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• pp.238-239
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• 2001

The egg-yolk precursor vitellogenin(VTG) is secreted by the liver of female and male fish, in response to estrogenic compound. Carp(Cryprinus carpio) vitellogenin of one major protein is 160kDa, two minor proteins is 110kDa and 170kDa. We were induced vitellogenin by inject of 17-estradiol and purified in a two step procedure by separating it from plasma protein precipitated by 35% saturated ${(NH_4)}_2SO_4$ and then from the remainder by Mono-Q chromatography. We found major carp(Cyprinus carpio) vitellogenin band at 160kDa. Effect of different concentration of oestrogen on vitellogenin synthesis in carp(Cyprinus carpio) exposed for 4 weeks. Show differential effects on vitellogenin synthesis 7 days after treatment. Plasma vitellogenin was measured 3 times for each by an enzyme linked immunosorbent assay(ELISA). ELISA was developed for the detection of the egg yolk precursor vitellogenin in plasma of carp(Cyprinus carpio). The ELISAS performance was optimized and characterized.

• Journal of Korean Society of Forest Science
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• v.95 no.1
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• pp.91-101
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• 2006

This study was conducted to analyze the relationships between distributions of major hardwood species and characteristics of topography in natural hardwood forest at Mt. Joongwang, Pyongchang-gun, Gangwon-do which belongs to mid-northern part of the temperate forest. Acer pictum var. mono, Acer manshuricum and Quercus mongolica were affected by elevation, while Ulmus davidiana var. japonica and Fraxinus rhynchophylla were affected by the change of slope. Importance values of A. pictum, Betula costata, Ulmus laciniata and Cornus controversa in valley were 13.41%, 5.68% and 3.29%, respectively, which were higher than in any other topography. Species diversity of valley was the highest with 0.98. Ratio of A. pictum and Q. mongolica in dominant species were 24% and 14%, respectively in valley, while were 19% and 23% in slop. Q. mongolica ratio in ridgeline was higher than any other species. Importance values of A. pictum increased continuously until elevation 1,200 m. importance values of F. rhynchophylla was 6.6% which was the highest value found on elevation 800 m. Distributions of major hardwood species were different according to the elevation. The ratio of Q. mongolica and A. pictum were 6.4%~17.5% and 6.0%~10.7% respectively, which were the highest ratio. Species diversity increased according to the increase of the elevation.

• Journal of Korean Society of Forest Science
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• v.110 no.1
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• pp.1-12
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• 2021

In this study, the structure of forest vegetation in Mt. Seondalsan, Bongwha-gun, was analyzed. Vegetation data were collected in 137 quadrat plots using the Z-M phytosociological method from June to October 2018. These data were analyzed using vegetation classification, importance value,and species diversity. Consequently, vegetation was classified as a Quercus mongolica community group that was divided into four communities: Cornus controversa, Phlomis umbrosa, Pinus densiflora, and Q. mongolica communities. The C. controversa community was subdivided into Magnolia sieboldii and Parthenocissus tricuspidata groups; the P. densiflora community was divided into Vaccinium hirtum var. koreanum, Quercus variabilis, and P. densiflora groups. In the C. controversa community, the M. sieboldii group was divided into the Acer mandshuricum and M. sieboldii subgroups, whereas the P. tricuspidata group was divided into the Larix kaempferi, Pinus koraiensis, and P. tricuspidata subgroups. In the P. densiflora community, the V. hirtum var. koreanum group was divided into the Rhododendron micranthum and V. hirtum var. koreanum subgroups. According to importance value analysis, C. controversa, L. kaempferi, P. koraiensis, Q. mongolica, Acer pictum subsp. mono, P. densiflora, and Q. variabilis were mainly indicated to have high value in the tree layer. The species diversity of Mt. Seondalsan was 1.969, which was greater than that of another Forest Genetic Resource Reserve.

• Korean Journal of Environment and Ecology
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• v.35 no.2
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• pp.204-219
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• 2021

This study was conducted to investigate and analyze the characteristics of the plant community structure of vegetation distributed on the western slope and ridge connecting Mt. Noja to Mt. Gara. This basic research was executed not only to restore and manage forest vegetation, but also to monitor the trend of change in the long term. As a result of classifying the communitise in 86 survey quadrats, the Pinus thunbergii-Platycarya strobilacea comm. and P. thunbergii-P. densiflora comm. were distributed around the lowlands. The Carpinus tschonoskii-Deciduous broad-leaved comm., Styrax japonicus-Deciduous broad-leaved comm., Acer pictum subsp. Mono-Deciduous broad-leaved comm., Deciduous broad-leaved comm., and Zelkova serrata comm. appeared in the valley and all stone areas. Quercus serrata comm., Q. serrata-S. japonicus comm., S. japonicus-Carpinus cordata comm., Euonymus oxyphyllus comm. were classified as being distributed on steep slopes with relatively high altitude. According to the succession trend of the forest, evergreen conifers will be transition to deciduous broad-leaved trees. However, deciduous broad-leaved arboreous forests, such as Carpinus tschonoskii, zelkova serrata, and Acer pictum subsp. Mono, were considered to maintain their current succession stage because not only the stratified structure was developed over about 50 years tree age, but also ecologically stabilized. As environmental factors, it was analyzed that altitude, pH, content of clay and silt, Mg⁺⁺, Ca⁺⁺, etc. directly or indirectly affect the distribution of plant communities.

• Korean Journal of Environment and Ecology
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• v.37 no.5
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• pp.347-366
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• 2023

In the present study, the vegetation was classified using the phytosociological method and canonical-correlation analysis (CCA) was implemented to analyze correlation between community structure and environmental factors in the natural habitats of forest byproducts, especially medicinal plants, such as Cudrania tricuspidata, Sorbus commixta, and Hovenia dulcis, in 2021-2022 to provide primary ecological data to establish environmental conditions for wild vegetable cultivation. A total of 11 plots in five regions, 8 plots in three regions, and 17 plots in 5 regions were selected for the natural habitats of C. tricuspidata in southern Korea, S. commixta in high mountains, and H. dulcis in valleys of central Korea, respectively. The importance value in each community was respectively analyzed as follows, in C. tricuspidata community, the importance value of C. tricuspidata (61.10) was the highest, followed by Celtis sinensis, Pinus thunbergii, Neolitsea aciculata, Styrax japonica, Carpinus coreana, Quercus serrata, and Q. acutissima. In Sorbus commixta community, Q. mongolica (57.21) was the highest, followed by, S. commixta (42.58), Betula ermani, Tilia amurensis, A. pseudosieboldianum, A. tschonoskii var. rubripes, Cornus controversa, Magnolia sieboldii, and Taxus cuspidata. In H. dulcis community, H. dulcis (64.58) was the highest, followed by Zelkova serrata, Cornus controversa, A. mono, Q. serrata, C. cordata, and Juglans mandshurica. As the result of the analysis on DBH of the major species having the high importance value, in C. tricuspidata community, C. tricuspidata, C. sinensis, Neolitsea aciculata, and C. coreana show the density of normal distribution, so the dominant status of these species is likely to continue. In S. commixta community, S. commixta show the density of reverse J-shaped curve, so the dominant status of these species is likely to be stable, and Q. mongolica, B. ermani and T. amurensis, show the density of normal distribution, so the dominant status of these species is likely to continue. In H. dulcis community, C. cordata, and J. mandshurica show the density of reverse J-shaped curve, so the dominant status of these species is likely to be stable, and H. dulcis, Z. serrata, C. controversa and A. mono had a formality distribution, suggesting a continuous domination of these species over the other species for the time being. The results of CCA ordination analysis using 11 environmental factors and 30 communities of three taxa classified by TWINSPAN analysis revealed that the altitude showed the strongest correlation with the vegetation. C. tricuspidata community was distributed on the moderate and gentle northeastern slope at low altitude with the highest pH, C.E.C, Ca²⁺, and Mg² and various P₂O₅, whereas S. commixta community was distributed on the steep slope at high altitude with the highest O.M and T-N and lower P₂O₅, Ca²⁺, Mg²⁺, C.E.C and pH, which is the opposite tendency of the environment of C. tricuspidata community. H. dulcis community was distributed on the gentle northern slope at lower altitude with an average pH, O.M, T-N, Ca²⁺, Mg²⁺, and C.E.C, except higher P₂O₅.

• Korean Journal of Environment and Ecology
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• v.31 no.2
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• pp.202-219
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• 2017

The purpose of this study was to analyze the community structure of forest vegetation in Mt. Geumsusan belong to Woraksan National Park for providing basic information of ecological management. Data were collected by 41 plots from June to November in 2016 using Z-M phytosociology method, which was analyzed with vegetation types, mean importance value and coincidence method. As the results, the forest vegetation was classified into Quercus mongolica community group including Cornus controversa community, Castanea crenata community(Vegetation unit 3), Quercus variabilis community(Vegetation unit 4) and Q. mongolica community(Vegetation unit 5). C. controversa community was divided into two groups such as Ulmus davidiana var. japonica group(Vegetation unit 1) and Parthenocissus tricuspidata group(Vegetation unit 2). Mean importance percentage of vegetation unit 1 was showing Fraxinus rhynchophylla 14.9%, Morus bombycis 8.7% and Acer pictum subsp. mono 8.3%, that of unit 2 was Larix kaempferi 23.2%, C. controversa 20.1% and P. tricuspidata 6.5%, that of unit 3 was Q. mongolica 15.8%, C. crenata 13.4% and F. rhynchophylla 9.8%, that of unit 4 was Q. mongolica 26.6%, Q. variabilis 20.8% and Pinus densiflora 16.7%, that of unit 5 was Q. mongolica 48.3%, Styrax obassia 7.5%, F. rhynchophylla 5.3% in the order, respectively. Each vegetation unit was classified with dependance on environmental factors as 700m of altitude, $20^{\circ}$ of slope degree, middle slope of topography, 20% of bare rock, 30 taxa of present species, 80% of tree layer coverage rate and 20m of tree layer height. In conclusion, it was preferentially considered that development of peculiar and specific management methods with vegetation unit classified above should be needed for ecological and sustainable forest vegetation management.

• Environmental Sciences Bulletin of The Korean Environmental Sciences Society
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• v.2 no.2
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• pp.149-158
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• 1998

Biomass of non-living brown seaweed Sargassun fluitans pretreated with NaOH is capable of taking up more than $10\%$ $(q_{max}$ : 3.85 mmol/g for Al and 1.48 mmol/g for Cu) of its dry weight in the Al and Cu at pH of 4.5. However, the maximum Al and Cu uptakes calculated from Langmuir isotherm were 1.58 mmol/g for Al and 1.35 mmol/g for Cu at pH 3.5. Equilibrium batch sorption study was performed using two-metal system containing Al and Cu. The mathematical model of the two-metal sorption system enabled quantitative estimation of one-metal biosorption inhibition due to the influence of a second metal. NaOH-treated S. fluitans contained 2.19 mmol $(43\;wt.\%)$ carboxyl groups per gram of biomass. A modified form of Langmuir, which assumes binding of Cu as $Cu^{2+}$ and Al as $Al(OH)_2^+,$ was used to model the experimental data. This result agrees with the one of mono-valent sorption for Al in single-metal system. The modified Langmuir model gives the following affinity correlated coefficients: 0.196 for Cu and 6.820 for Ah at pH 4.5, and 2.904 for Cu and 3.131 for Al at pH 3.5. The interference of Al in Cu biosorptive uptake was assessed by `cutting' the three dimensional uptake isotherm surfaces at constant second-metal final concentrations. Equimolar final equilibrium concentrations of Cu and Al of 1 mM at pH 4.5 give Cu and hi uptakes reduced by $82.5\%\;and\;5.4\%,$ respectively. However, these values at pH 3.5 were $55\%\;(Cu)\;and\;31\%$ (Al).

• Asian-Australasian Journal of Animal Sciences
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• v.26 no.11
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• pp.1577-1582
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• 2013

The effects of dietary supplementation of selenium (Se), iodine (I), and a combination of both on the blood haematology, serum free thyroxine (FT4) and free triiodothyronine (FT3) hormones and glutathione peroxidase enzyme (GSH-Px) activity were examined on twenty four (7 to 8 months old, $22{\pm}1.17$ kg live weight) Kacang crossbred male goats. Animals were randomly assigned to four dietary treatments (6 animals in each group). Throughout 100 d of feeding trial, the animals of control group (CON) received a basal diet, while the other three groups were offered basal diet supplemented with 0.6 mg/kg diet DM Se (SS), or 0.6 mg/kg diet DM I (PI), or a combination of both Se and I, each at 0.6 mg/kg diet DM (SSPI). The haematological attributes which are haemoglobin (Hb), red blood cell (RBC), packed cell volume (PCV), mean cell volume (MCV), white blood cells (WBC), band neutrophils (B Neut), segmented neutrophils (S Neut), lymphocytes (Lymph), monocytes (Mono), eosinophils (Eosin) and basophils (Baso) were similar among the four treatment groups, while serum levels of Se and I increased significantly (p<0.05) in the supplemented groups. The combined dietary supplementation of Se and I (SSPI) significantly increased serum FT3 in the supplemented animals. Serum GSH-Px activity increased significantly in the animals of SS and SSPI groups. It is concluded that the dietary supplementation of inorganic Se and I at a level of 0.6 mg/kg DM increased serum Se and I concentration, FT3 hormone and GSH-Px activity of Kacang crossbred male goats.