• Title/Summary/Keyword: Molecular Characteristics

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Molecular Phylogeny Reconstruction of Grouper (Serranidae: Epinephelinae) at Northern Part of Bird's Head Seascape - Papua Inferred from COI Gene

  • Tapilatu, Ricardo F.;Tururaja, Tresia Sonya;Sipriyadi, Sipriyadi;Kusuma, Aradea Bujana
    • Fisheries and Aquatic Sciences
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    • v.24 no.5
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    • pp.181-190
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    • 2021
  • Grouper is one of the most economically important fishes with various morphological forms and characteristics, meaning it is often difficult to identify species and distinguish between life stages, sometimes leading to morphological misidentification. Therefore, identification using a molecular deoxyribose nucleic acid (DNA) approach was needed as an alternative means to identify closely related species. This study aims to determine the molecular phylogeny of grouper from the northern part of the Bird's Head Seascape of Papua. The DNA sequence of each cytochrome oxidase I (COI) gene was used to study the molecular relationship among closely related species of grouper. The results showed that there were 16 Epinephelinae that have been compared to a gene bank (National Centre for Biotechnology Information, NCBI) in the sequence length of 623 base pairs. The closest genetic distance was found between Cephalopholis miniata and Cephalopholis sexmaculata (0.036), while the furthest genetic distance was observed between Plectropomus laevis and Cephalopholis spiloparaea (0.247). This finding was further reinforced by the morphological characters of each species. This finding highlighted that five genera were represented as a monophyletic group (clade), i.e., Epinephelus, Cephalopholis, Plectropomus, Saloptia and Variola.

DED Interaction of FADD and Caspase-8 in the Induction of Apoptotic Cell Death

  • Park, Young-Hoon;Han, Chang Woo;Jeong, Mi Suk;Jang, Se Bok
    • Journal of Microbiology and Biotechnology
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    • v.32 no.8
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    • pp.1034-1040
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    • 2022
  • Fas-associated death domain (FADD) is an adapter molecule that bridges the interaction between receptor-interacting protein 1 (RIP1) and aspartate-specific cysteine protease-8 (caspase-8). As the primary mediator of apoptotic cell death, caspase-8 has two N-terminal death-effector domains (DEDs) and it interacts with other proteins in the DED subfamily through several conserved residues. In the tumor necrosis receptor-1 (TNFR-1)-dependent signaling pathway, apoptosis is triggered by the caspase-8/FADD complex by stimulating receptor internalization. However, the molecular mechanism of complex formation by the DED proteins remains poorly understood. Here, we found that direct DED-DED interaction between FADD and caspase-8 and the structure-based mutations (Y8D/I128A, E12A/I128A, E12R/I128A, K39A/I128A, K39D/I128A, F122A/I128A, and L123A/I128A) of caspase-8 disrupted formation of the stable DED complex with FADD. Moreover, the monomeric crystal structure of the caspase-8 DEDs (F122A/I128A) was solved at 1.7 Å. This study will provide new insight into the interaction mechanism and structural characteristics between FADD and caspase-8 DED subfamily proteins.

Biochemical Characteristics of Cd-binding High Molecular Weight Proteins (카드뮴 결합 고분자량 단백질의 생화학적 특성)

  • 천기정;김봉희
    • YAKHAK HOEJI
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    • v.39 no.4
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    • pp.380-384
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    • 1995
  • The isoelelectric points of Cd-BP(l) and Cd-BP(II), cadmium-binding proteins, were 6.01 and 5.35, respectively. Both of them contained zinc. As for the amino acid composition, Cd-BP(I) contained a lot of glycine and lysine but none of such aromatic amino acids as tyrosine and phenylalanine.. On the other hand, Cd-BP(II) contained leucine, histidine, asparti cacid and alanine but no aromatic amino acids.

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A Study on Preparation of Industrial Polyaluminium Sulfate (공업용 포리황산알미늄의 제조에 관한 연구)

  • 육창규;이내우
    • Journal of Environmental Science International
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    • v.5 no.2
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    • pp.203-210
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    • 1996
  • To improve coagulation characteristics and to reduce dossage of chemicals in watertreatment, polyaluminium sulfate was manufactured by reaction of polymerization between aluminium sulfate and sodium aluminate. As the results of affirmation and performance test, the yield of product was about 24% from molecular cut on levels by ultrafilteration method. The type of molecular is nearly affirmated as a kind of complex like AIm(OH)n(3m-n)+ from Scanning Electron Microscopy (S.E.M.) and X-ray diffraction test. Since the synthesized polymer was supposed to be quasi, further study for stabilization was required.

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A Study for the Dielectric Properties Dependent on Frequency and Temperature in Polymerzed Materials (합성수지의 유전주파수 및 온도특성에 대한 고찰)

  • 김봉흡;이준웅
    • 전기의세계
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    • v.19 no.5
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    • pp.8-16
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    • 1970
  • The characteristics of dielectric absorption and dispersion in polymerized materials produced in Korea was investigated in MHz band and at several sets of temperature. For this study the theoretical basis stood on the Cole-Cole's empirical formula and it has been extended to the form convenient to investigate the observation. As the result, it has been confirmed that in most cases, two or much more sorts of dipole participate in dielectric propertics of these polymers and the materials containing aromatic molecular structure have, in general, greater loss angle than those constructed chain type molecular structure.

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A study on a Boron-Nitride Nanotube as a Gigahertz Oscillator (기가헤르츠 오실레이터를 위한 BN 나노튜브 연구)

  • Lee, Jun-Ha
    • Journal of the Semiconductor & Display Technology
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    • v.6 no.1 s.18
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    • pp.27-30
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    • 2007
  • The gigahertz oscillator behavior of double-walled boron-nitride nanotube (BNNT) was investigated by using classical molecular dynamics simulations. The BNNT oscillator characteristics were compared to carbon-nanotube (CNT) and hybrid-C@BNNT oscillators. The results show that the BNNT oscillators are higher than the van der Waals force of the CNT oscillator. Since the frictional effects of BNNT oscillators are higher than that of a CNT oscillator, the damping factors of BNNT and hybrid oscillators are higher than that of a CNT oscillator.

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A Comparative study for single-shot immunization of diphtheria toxoid with combined PLGA microspheres.

  • Yoon, Mi-Kyeung;Lee, Jung-Min;Kim, Hee-Kyu;Choi, Young-Wook
    • Proceedings of the PSK Conference
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    • 2002.10a
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    • pp.416.1-416.1
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    • 2002
  • Biodegradable PLGA microspheres(MS) have been widely studied for delivering antigens because PLGA has the characteristics of various degradation rate. In general. since MS have shown potential for single-dose vaccines. the degradation rate of PLGA is determined by their molecular weight. polymer composition, etc. We studied the influences of molecular weight of PLGA. polymer composition and surfactant on in vitro release and in vivo effects. (omitted)

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Micro/Nano Adhesion and Friction Characteristics of PTFE Coating Film Deposited by IBAD Method (IBAD 방법으로 코팅된 PTFE 박막의 마이크로/나노 응착 및 마찰 특성)

  • 윤의성;오현진;한흥구;공호성;장경영
    • Tribology and Lubricants
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    • v.20 no.5
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    • pp.237-244
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    • 2004
  • Micro/nano tribological characteristics of PTFE coating films were experimentally studied. PTFE (polytetrafluoroethylene) modified polyethylene and low molecular weight PTFE were used as a coating materials. These films were deposited on Si-wafer (100) by IBAD (ion beam assisted deposition) method. The Ar ion beam sputtering was performed to change the surface topography of films using a hollow cathode ion gun under different Ar ion dose conditions in a vacuum chamber. Micro/nano tribological characteristics, water wetting angles and roughness were measured with a micro tribotester, SPM (scanning probe microscope), contact anglemeter and profilometer, respectively. The durability of the films were measured with macro tribotester. Results showed that the PTFE coating surfaces were converted to hydrophobic. The water contact angle of coated surfaces and surface roughness increased with the coating thickness. Adhesion and friction in micro and nano scale were governed by magnitude of normal load in soft material such as PTFE films. As the increase of sputtering time on low molecular weight PTFE films, the surface roughness was increased and nano adhesion and friction were decreased. The nano tribological characteristics of surfaces are mainly improved by chemical modification such as PTFE coating and given a synergy effect by the physical modification such as topographic modification.