• Journal of Microbiology and Biotechnology
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• v.23 no.3
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• pp.364-374
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• 2013

Monacolin K and yellow pigment, produced by Monascus sp., have each been proven to be beneficial compounds as antihypercholesterolemic and anti-inflammation agents, respectively. However, citrinin, a human toxic substance, was also synthesized in this fungus. In this research, solidstate fermentation of M. purpureus TISTR 3541 was optimized by statistical methodology to obtain a high production of monacolin K and yellow pigment along with a low level of citrinin. Fractional factorial design was applied in this study to identify the significant factors. Among the 13 variables, five parameters (i.e., glycerol, methionine, sodium nitrate, cultivation time, and temperature) influencing monacolin K, yellow pigment, and citrinin production were identified. A central composite design was further employed to investigate the optimum level of these five factors. The maximum production of monacolin K and yellow pigment of 5,900 mg/kg and 1,700 units/g, respectively, and the minimum citrinin concentration of 0.26 mg/kg were achieved in the medium containing 2% glycerol, 0.14% methionine, and 0.01% sodium nitrate at $25^{\circ}C$ for 16 days of cultivation. The yields of monacolin K and yellow pigment were about 3 and 1.5 times higher than the basal medium, respectively, whereas citrinin was dramatically reduced by 36 times.

• Advances in aircraft and spacecraft science
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• v.3 no.2
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• pp.113-148
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• 2016

In a whole variety of higher order plate theories existing in the literature no consideration is given to the transverse normal strain / deformation effects on flexural response when these higher order theories are applied to shear flexible composite plates in view of minimizing the number of unknown variables. The objective of this study is to carry out cylindrical bending of simply supported laminated composite and sandwich plates using sinusoidal shear and normal deformation plate theory. The most important feature of the present theory is that it includes the effects of transverse normal strain/deformation. The displacement field of the presented theory is built upon classical plate theory and uses sine and cosine functions in terms of thickness coordinate to include the effects of shear deformation and transverse normal strain. The theory accounts for realistic variation of the transverse shear stress through the thickness and satisfies the shear stress free conditions at the top and bottom surfaces of the plate without using the problem dependent shear correction factor. Governing equations and boundary conditions of the theory are obtained using the principle of minimum potential energy. The accuracy of the proposed theory is examined for several configurations of laminates under various static loadings. Some problems are presented for the first time in this paper which can become the base for future research. For the comparison purpose, the numerical results are also generated by using higher order shear deformation theory of Reddy, first-order shear deformation plate theory of Mindlin and classical plate theory. The numerical results show that the present theory provides displacements and stresses very accurately as compared to those obtained by using other theories.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.41 no.2
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• pp.178-188
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• 2016

The traffic in the wired and wireless networks has increased exponentially because of increase of smart phone and improvement of PC performance. Multimedia services and file transmission such as Facebook, Youtube occupy a large part of the traffic. CDN is a technique that duplicates the contents on a remote web server of content provider to local CDN servers near clients and chooses the optimal CDN server for providing the content to the client in the event of a content request. In this paper, the content request message between CDN servers and the client used the SCRP algorithm utilizing the MST algorithm and the traffic throughput was optimized. The average response time for the content request is reduced by employing HC_LRU cache algorithm that improves the cache hit ratio. The proposed SCRP and HC_LRU algorithm may build a scalable content delivery network system that efficiently utilizes network resources, achieves traffic localization and prevents bottlenecks.

• Journal of the Korean Society of Safety
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• v.29 no.1
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• pp.47-53
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• 2014

The application of advanced Main Control Room(MCR) is accompanied with lots of changes and different forms and features through the virtue of new digital technologies. The characteristics of these digital technologies and devices give many opportunities to the interface management, and can be integrated into a compact single workstation in advanced MCR so that workers can operate the plant with minimum physical burden under any operation conditions. However, these devices may introduce new types of human errors and thus a means to evaluate and prevent such errors is needed, especially those related to characteristics of digital devices. This paper reviewed the new type of human error hazards of tasks based on digital devices and surveyed researches on physiological assessment related to human error. An experiment was performed to verify human error hazards by physiological responses such as EEG which was measured to evaluate the cognitive workload of operators. And also, the performances of four tasks which are representative in human error hazard tasks based on digital devices were compared. Response time, ${\beta}$ power spectrum rate of each task by EEG, and mental workload by NASA-TLX were evaluated. In the results of the experiment, the rate of the ${\beta}$ power was increased in the task 1 and task 4 which are searching and navigating task and memory task of hierarchical information, respectively. In case of the mental workload, in most of evaluation items, task 1 and 4 were highly rated comparatively. In this paper, human error hazards might be identified by highly cognitive workload. Conclusively, it was concluded that the predictive method which is utilized in this paper and an experimental verification can be used to ensure the safety when applying the digital devices in Nuclear Power Plants (NPPs).

• Mycobiology
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• v.45 no.4
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• pp.240-254
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• 2017

Cereal grains are the most important food source for humans. As the global population continues to grow exponentially, the need for the enhanced yield and minimal loss of agricultural crops, mainly cereal grains, is increasing. In general, harvested grains are stored for specific time periods to guarantee their continuous supply throughout the year. During storage, economic losses due to reduction in quality and quantity of grains can become very significant. Grain loss is usually the result of its deterioration due to fungal contamination that can occur from preharvest to postharvest stages. The deleterious fungi can be classified based on predominance at different stages of crop growth and harvest that are affected by environmental factors such as water activity ($a_w$) and eco-physiological requirements. These fungi include species such as those belonging to the genera Aspergillus and Penicillium that can produce mycotoxins harmful to animals and humans. The grain type and condition, environment, and biological factors can also influence the occurrence and predominance of mycotoxigenic fungi in stored grains. The main environmental factors influencing grain fungi and mycotoxins are temperature and $a_w$. This review discusses the effects of temperature and $a_w$ on fungal growth and mycotoxin production in stored grains. The focus is on the occurrence and optimum and minimum growth requirements for grain fungi and mycotoxin production. The environmental influence on aflatoxin production and hypothesized mechanisms of its molecular suppression in response to environmental changes are also discussed. In addition, the use of controlled or modified atmosphere as an environmentally safe alternative to harmful agricultural chemicals is discussed and recommended future research issues are highlighted.

• Biomolecules & Therapeutics
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• v.27 no.1
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• pp.48-53
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• 2019

Reactive oxygen species (ROS) are widely generated in biological processes such as normal metabolism and response to xenobiotic exposure. While ROS can be beneficial or harmful to cells and tissues, generation of ROS by diverse anti-cancer drugs or phytochemicals plays an important role in the induction of apoptosis. We recently identified a derivative of naphthalene, MS-5, that induces apoptosis of an ovarian cell, CAOV-3. Interestingly, MS-5 induced apoptosis by down-regulating the ROS. Cell viability was evaluated by water-soluble tetrazolium salt (WST-1) assay. Apoptosis was evaluated by flow cytometry analysis. Intracellular ROS ($H_2O_2$), mitochondrial superoxide, mitochondrial membrane potential (MMP) and effect on cycle were determined by flow cytometry. Protein expression was assessed by western blotting. The level of ATP was measured using ATP Colorimetric/Fluorometric Assay kit. MS-5 inhibited growth of ovarian cancer cell lines, CAOV-3, in a concentration- and time-dependent manner. MS-5 also induced G1 cell cycle arrest in CAOV-3 cells, while MS-5 decreased intracellular ROS generation. In addition, cells treated with MS-5 showed the decrease in MMP and ATP production. In this study, we found that treatment with MS-5 in CAOV-3 cells induced apoptosis but decreased ROS level. We suspect that MS-5 might interfere with the minimum requirements of ROS for survival. These perturbations appear to be concentration-dependent, suggesting that MS-5 may induce apoptosis by interfering with ROS generation. We propose that MS-5 may be a potent therapeutic agent for inducing apoptosis in ovarian cancer cell through regulation of ROS.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.17 no.2
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• pp.520-541
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• 2023

In CRNs, SS is of utmost significance. Every CR user generates a sensing report during the training phase beneath various circumstances, and depending on a collective process, either communicates or remains silent. In the training stage, the fusion centre combines the local judgments made by CR users by a majority vote, and then returns a final conclusion to every CR user. Enough data regarding the environment, including the activity of PU and every CR's response to that activity, is acquired and sensing classes are created during the training stage. Every CR user compares their most recent sensing report to the previous sensing classes during the classification stage, and distance vectors are generated. The posterior probability of every sensing class is derived on the basis of quantitative data, and the sensing report is then classified as either signifying the presence or absence of PU. The ISVM technique is utilized to compute the quantitative variables necessary to compute the posterior probability. Here, the iterations of SVM are tuned by novel GO-PSA by combining GOA and PSO. Novel GO-PSA is developed since it overcomes the problem of computational complexity, returns minimum error, and also saves time when compared with various state-of-the-art algorithms. The dependability of every CR user is taken into consideration as these local choices are then integrated at the fusion centre utilizing an innovative decision combination technique. Depending on the collective choice, the CR users will then communicate or remain silent.

• Advances in nano research
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• v.16 no.6
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• pp.623-638
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• 2024

This study investigates the thermal post-buckling behavior of concrete eccentric annular sector plates reinforced with graphene oxide powders (GOPs). Employing the minimum total potential energy principle, the plates' stability and response under thermal loads are analyzed. The Haber-Schaim foundation model is utilized to account for the support conditions, while the transform differential quadrature method (TDQM) is applied to solve the governing differential equations efficiently. The integration of GOPs significantly enhances the mechanical properties and stability of the plates, making them suitable for advanced engineering applications. Numerical results demonstrate the critical thermal loads and post-buckling paths, providing valuable insights into the design and optimization of such reinforced structures. This study presents a machine learning algorithm designed to predict complex engineering phenomena using datasets derived from presented mathematical modeling. By leveraging advanced data analytics and machine learning techniques, the algorithm effectively captures and learns intricate patterns from the mathematical models, providing accurate and efficient predictions. The methodology involves generating comprehensive datasets from mathematical simulations, which are then used to train the machine learning model. The trained model is capable of predicting various engineering outcomes, such as stress, strain, and thermal responses, with high precision. This approach significantly reduces the computational time and resources required for traditional simulations, enabling rapid and reliable analysis. This comprehensive approach offers a robust framework for predicting the thermal post-buckling behavior of reinforced concrete plates, contributing to the development of resilient and efficient structural components in civil engineering.

• Journal of Forest and Environmental Science
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• v.40 no.2
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• pp.99-110
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• 2024

According to the REDD+ program, it is necessary to monitor, quantify, and report forest conditions in protected land areas. The objectives of this work were to quantify the average monthly aerial net primary productivity (ANPP_MONTH) of semi-arid Chaco Forest at Copo National Park (CNP), Santiago del Estero, Argentina, during the period 2000-2023, as well as its spatial distribution and relationship, and its use efficiency (RUE) of average monthly rainfall (AMR). The ANPP_MONTH model accounted for 90% of the seasonal variability from October to May, the average seasonal ANPP_MONTH was 145 tons of dry matter per hectare (t dm/ha), being the maximum in January with 192 t dm/ha and the minimum in May with 91 t dm/ha. The surface area covered by ANPP_MONTH exhibited a consistent positive trend from October to May (t test=15.65, p<0.01). Strong and significant direct relationships were found between ANPP_MONTH and AMRs, linear models explaining 90% and 96% of the variability, respectively. The results obtained become reference values for assessing the capacity of the forest systems to stock carbon for global warming mitigation and for monitoring and controlling their response to extreme climatic adversities. The average ANPP_MONTH reduces uncertainty when defining the thresholds to monitor and quantify ANPP and forest area, thus facilitating the detection of negative changes in land use in CNP. Such results evidence the National Parks Administration's high effectiveness for the maintenance of protected area and for the high ANPP of the FCHS of CNP in the period 2000-2023.

• The Journal of the Korean Society for Microbiology
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• v.21 no.1
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• pp.133-144
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• 1986

This study was undertaken to assess the effect of ginseng administration on T lymphocyte induced local xenogenic graft-versus-host(GVM) reactions which were induced with thymocyte, spleen cell and lymph node cell of ICR mice. Mice received daily 10mg of 70% alcohol ginseng extract oral1y for 100days and control mice remained untreated for the same period of time. The cells from donor mice were injected intradermally into the closely shaven abdominal skin of Sprague-Dawley rats for GVH tests. The thymocyte from control(ginseng-untreated) mice showed a negative local GVH reaction, whereas thymocyte from experimental(ginseng-treated) mice showed a positive reaction with the rate of 17.4%. When spleen cells were injected, the incidence of positive local GVH reaction was 66.7% among ginseng-treated mice, as opposed to incidence of 45.5% of positive local GVH reaction among control mice. The incidence of positive local GVH reaction of the lymph node cells when injected into a recipient was 71.4% among ginseng-treated mice as compared with that of 18.9% among control mice. The relationship between spleen cell inoculum and intensity of the local GVH reaction was assessed in ginseng-untreated mice. The intensity of GVH reaction clearly appears to be dose related. In ginseng-treated mice, a minimum of $1{\times}10^7$ spleen cell was required for production of positive local GVH reaction with almost linear relationship up to an inoculum of $5{\times}10^8$ cells. In control mice, however, a minimum of $1{\times}10^8$ spleen cells was required for positive GVH reaction. These results strongly suggest that the ginseng administration augments significantly the local xenogenic GVH reaction which was used to assess T lymphocyte function and immunocompetence of mice and in addition to this, these results appear to support previous suggestions that the local GVH reaction consitutes a qualitative test of the functional activity of T lymphocytes. These results may be the first to induce local GVH reaction, employing rats as recipient and mice as donor. This study was also desingned to investigate some of the effects of ginseng extract on lymphocyte-macrophage interactions. This was accomplished by in vitro quantification of 1) migratory inhibitory factor(MIF) synthetic capacity of splenic lymphocytes in mice previously primed with ginseng 2) MIF responsiveness of mouse peritoneal macrophages or chicken peripheral leucocytes under the presence of ginseng extract 3) migration ability of chicken peripheral leucocytes by direct stimulation of ginseng extract or ginseng saponin and 4) immunosuppressive effects of immunosuppressants such as cyclophosphamide, cyclosporin A or dexamethasone. Mice divided equally into the ginseng and the saline groups, which received intraperitoneally daily 0.2ml of ginseng absolute alcohol-extract(5mg/ml) and same amount of saline for 15 days, respectively. The cellular immune responsiveness of these mice was assayed 15 days after ginseng pretreatment. Splenic lymphocytes of mice treated with ginseng, when stimulated with sensitized specific-antigen such as sheep red blood cells or toxoplasmin, or with polyclonal activator concanavalin A, produced significantly more MIF than those of control saline group. MIF responsiveness of normal mouse macrophages was significantly augmented when assayed under the presence of ginseng extract (1mg/ml). The migratory ability of normal chicken leucocytes in the absence of MIF was significantly decreased by the stimulation of ginseng extract alone. MIF response was significantly decreased by immunosuppressants and this impaired response was not restored by ginseng pretreatment. This study was additionally performed to evaluate the effect of ginseng on the expulsion of adult Trichinella spiralis in mice. ICR mice were infected experimentally by esophageal incubation of 300 T. spiralis infective muscle larvae prepared by acid-pepsin digestion of infected mice. and received oral administration of 70% alcohol ginseng extract(10mg/mouse/day) for the indicated days plus 4 days before infection. At various times after infection, the number of adult T. spiralis worms in small intestines was determined. Interestingly, ginseng-treatment was accompanied by accelerated expulson of T. spiralis. These results led to the conclusion that Panax ginseng caused some enhancing effect on GVH reaction, macrophage migration inhibition reaction and expulsion of T. spiralis. In addition these results suggested that the mechanisms responsible for this enhancement of ginseng may be chiefly or partially due to nonspecific stimulation of cell-mediated immune response.