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Temperature-dependent Index of Mitotic Interval (${\tau}_0$) for Chromosome Manipulation in Korean Bullhead, Pseudobagrus fulvidraco

  • Lim, Sang Gu;Han, Hyoung Kyun;Gil, Hyun Woo;Park, In-Seok
    • Development and Reproduction
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    • v.16 no.4
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    • pp.321-327
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    • 2012
  • Korean bullhead (Pseudobagrus fulvidraco) was collected from the Kum River areas of Kangkyung-eup, Nonsan city, Chungcheongnam-do, Korea, from April to June, 2012 and was fertilized in order to observe egg development and temperature-related cleavage rates and mitotic intervals (${\tau}_0$). The fertilized eggs were separative, demersal and light yellowish with $1.5{\pm}0.06mm$ in diameter, and did not contain oil globules. The first cleavage stages were 90 min, 80 min, 60 min and 50 min at $21^{\circ}C$, $24^{\circ}C$, $27^{\circ}C$ and $30^{\circ}C$, respectively. At higher temperatures, eggs developed faster and underwent further identical development. For Korean bullhead, ${\tau}_0$ were $33.4{\pm}2.08$ min at $21^{\circ}C$, $31.5{\pm}3.06$ min at $24^{\circ}C$, $28.1{\pm}2.11$ min at $27^{\circ}C$ and $26.4{\pm}3.35$ min at $30^{\circ}C$. There were strong negative correlations between the $\tau_0$ and water temperatures at all points studied (Y=-1.13X+58.15, $R^2$=0.98, n=30, where Y is ${\tau}_0$ and X is temperature). The results obtained in this work will be helpful for chromosome manipulation by use of cleavage frequency data and ${\tau}_0$ data in Korean bullhead.

Infiltration Rate of Some Upland Soils in Korea (우리나라 밭토양의 수분침투속도(水分浸透速度)에 관하여)

  • Jung, Y.S.;Ryu, K.S.;Im, J.N.
    • Korean Journal of Soil Science and Fertilizer
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    • v.13 no.1
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    • pp.1-6
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    • 1980
  • The infiltration rates of the upland soils on hill side slope were investigated in situ using rainulator of which rainfall intensity was 100mm/hr. The soil moisture profile after the water infiltration was compared with that calculated from Youngs' equation. The results obtained are as follows: 1. Time required for infiltration rate to reach constant during rainfall was 15 to 25 min. The infiltration rate measured after 30 min was considered to be final infiltration rate. 2. The final infiltration rates of clay soils were lower than 10mm/hr., loamy soils 10 to 20., coarse loamy soils 20 to 30, and sandy soils higher than 30mm/hr., respectively. 3. The saturated hydraulic conductivity of the surface soil of Samgag sandy loam was 0.47mm/min., Songjeong clay loam0.16 mm/min., and Jeonnam silty clay loam 0.14mn/min., respectively. 4. The soil moisture profile calculated from Young's equation was in close agreement with measured in situ.

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Comparative Anesthetic Effect as Propofol Infusion Rate During Maintenance of Anesthesia in Dog (개에서 Propofol 점적투여율 변화에 따른 마취효과)

  • 장광호;임재현;장인호
    • Journal of Veterinary Clinics
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    • v.17 no.1
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    • pp.109-120
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    • 2000
  • To evaluate anesthetic effecto of propofol infusion after premedication with xylazine, 20 days were randomly assigned 4 groups. Propofol was infused (group 1: 0.2 mg/kg/min, group 2 : 0.4 mg/kg/min, group 3 : 0.6 mg/kg/min, group 4 : 0.8 mg/kg/min) for a period of 90 minute immediately after premedication with xylazine(1 mg/kg) and atropine(0.05mg/kg) under oxygen supplementation. Induction of anesthesia was rapid and smooth providing satisfactory conditions for intubation in all the dogs. No vomiting and cyanosis were observed after induction and during propofol infustion. There was pain reflex in group 1 but not in group 3 and 4. Mean arousal times (mins) were $6.18{\pm}3.65(group 1), 13.07{\pm}5.05(group 2), 22.06{\pm}6.48(group 3) and 23.33{\pm}9.28 (group 4) and Mean walking times were 16.20{\pm}6.15(group 1), 15.80{\pm}4.73(group 2), 28.27{\pm}7.55 (group 3), 39.10{\pm}13.75$ (group 4) respectively. In group 4, body temperature during total infusion period in group 3, 4. Hematologic values (WBC, RBC, PCV) and serum chemistry values(ALT, AST, BUN, creatinite) were monitored before anesthesia, 1 hour and 1 day after termination of infusion postanestesia. No significant changes were monitored in all experimental group. Although propofol infusions of 0.2 mg/kg/min and 0.4mg/kg/min were considered too low to maintain a suitable depth of anesthesia, but that of 0.6mg/kg/min were considered too low to maintain a suitable depth of anesthesia, but that of 0.6mg/kg/min proper to provide a light planes for minor surgical procedure during 90 minutes with xylazine premedication.

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MAX-MIN Flow Control Supporting Dynamic Bandwidth Request of Sessions (세션의 동적 대역폭 요구를 지원하는 최대-최소 흐름제어)

  • Cho, Hyug-Rae;Chong, Song;Jang, Ju-Wook
    • Journal of Institute of Control, Robotics and Systems
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    • v.6 no.8
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    • pp.638-651
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    • 2000
  • When the bandwidth resources in a packet-switched network are shared among sessions by MAX-MIN flow control each session is required to transmit its data into the network subject to the MAX-MIN fair rate which is solely determined by network loadings. This passive behavior of sessions if fact can cause seri-ous QoS(Quality of Service) degradation particularly for real-time multimedia sessions such as video since the rate allocated by the network can mismatch with what is demanded by each session for its QoS. In order to alleviate this problem we extend the concept of MAX-MIN fair bandwidth allocations as follows: Individual bandwidth demands are guaranteed if the network can accommodate them and only the residual network band-width is shared in the MAX-MIN fair sense. On the other hand if sum of the individual bandwidth demands exceeds the network capacity the shortage of the bandwidth is shared by all the sessions by reducing each bandwidth guarantee by the MAX-MIN fair division of the shortage. we present a novel flow control algorithm to achieve this extended MAX-MIN fairness and show that this algorithm can be implemented by the existing ATM ABR service protocol with minor changes. We not only analyze the steady state asymptotic stability and convergence rate of the algorithm by appealing to control theories but also verify its practical performance through simulations in a variety of network scenarios.

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Slope and Forest Fuel Effect on Spreading of Forest Fire (산불 확산에 영향을 미치는 임지내 산림연료와 경사도에 관한 연구)

  • 채희문;이찬용
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.5 no.3
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    • pp.179-184
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    • 2003
  • This study investigated the relationship of fuel weight and depth together with slope on the spread of forest fire. Fire spread was faster on the greater slope in forested land. Fire had a greater spread rate with lighter fuel weight. The thickness of the fuel bed and forest fire spread rate were not related. The fire spread rate was closely related to the slope and weight of the fuel bed (significant at 0.01, 0.05, respectively). The thickness of the fuel bed was not significant (0.05).

The effect of Acanthopanax sessiliflorus using the model of neuropathic pain and formalin-induced pain. (신경병리성 통증과 포르말린 테스트 통증 모델을 이용한 오가피(五加皮)의 효과)

  • Kim, Jang-Hyun;Chang, Gyu-Tae;Kang, Mi-Sun
    • The Journal of Korean Medicine
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    • v.28 no.3 s.71
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    • pp.261-272
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    • 2007
  • Objectives : This study was conducted to determine the analgesic effect of Acanthopanax sessiliflorus using the model of neuropathic pain and formalin-induced pain. Methods : A model of neuropathic pain was made by injuring the tibial nerve and sural nerve while the common peroneal nerve was maintained. After 2 weeks, the Acanthopanax sessiliflorus was orally administered to rats. The author performed behavioral teststo try out mechanical allodynia using von frey filament and cold allodynia using acetone, which are calculated by counting withdrawal response on foot. Thirty minutes after the Acanthopanax sessiliflorus injection in the abdominal cavity, the formalin test was performed. 2% formalin in a volume of $20{\mu}l$was injected subcutaneously into the plantar surface of the hindpaw with 26-G needle. To access formalin-induced pain behavior, paw licking time was measured every 5 min. Results : The Acanthopanax sessiliflorus 400mg/10ml/kg group showed significant decrease the withdrawal response of mechanical allodynia using von frey filament in the 10min, 30min, 60min and 120min increments compared with the control group. There were no significant differences in each group in the withdrawal response of cold allodynia using acetone. The Acanthopanax sessiliflorus group showed significant decrease in the formalin-induced pain behavior in the 15min, 20min and 25min increments compared with the control group. Conclusions : The Acanthopanax sessiliflorus may have a significant analgesic effect on the general pain as well as nerve injury pain.

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Effect of an Electric Field on the AC Electrical Treeing in Various Epoxy/Reactive Diluent Systems

  • Bang, Jeong-Hwan;Park, Jae-Jun
    • Transactions on Electrical and Electronic Materials
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    • v.14 no.6
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    • pp.308-311
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    • 2013
  • The effect of an electric field on the ac electrical treeing in various epoxy/reactive diluent systems was studied in a needle-plate electrode geometry. Diglycidyl ether of bisphenol A (DGEBA) type epoxy was used as a base resin, and 1,4-butanediol diglycidyl ether (BDGE) or polyglycol (PG) as a reactive diluent was introduced to the DGEBA system, in order to decrease the viscosity of the DGEBA epoxy system. BDGE was acted as a chain extender, and PG acted as a flexibilizer, after the curing reaction. To measure the treeing initiation time and the propagation rate, three constant alternating currents (ac) of 10, 13 and 15 kV/4.2 mm (60 Hz) were applied to the specimen, in a needle-plate electrode arrangement, at $30^{\circ}C$ of insulating oil bath. When 10 kV/4.2 mm (60 Hz) was applied, the treeing initiation time and the propagation rate in the DGEBA system were 356 min and $1.10{\times}10^{-3}$ mm/min, respectively, those in the DGEBA/BDGE system were 150 min and $1.14{\times}10^{-3}$ mm/min, respectively. Those in the DGEBA/PG system were 469 min and $1.05{\times}10^{-3}$ mm/min, respectively. As 15 kV/4.2 mm (60 Hz) was applied, the propagation rate in the DGEBA system was $5.41{\times}10^{-3}$ mm/min, and that in the DGEBA/PG system was $1.42{\times}10^{-3}$ mm/min. These values meant that PG could be used as a reactive diluent in the DGEBA system, without the deterioration of the insulation breakdown property.

A Study on the Effect of Multi-Application of Fluoride on Enamel Remineralization (불화물 복합적용이 법랑질의 재석회화에 미치는 영향에 관한 연구)

  • Cho, Min-Jung;Lee, Hyang-Lim
    • Journal of Korean society of Dental Hygiene
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    • v.1 no.1
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    • pp.125-132
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    • 2001
  • This study was carried out to investigate the effect of multi or single application of fluoride plus 0.05% NaF solution on the remineralization of dental caries lesion. The microhardness changes of enamel surface were measured after application of fluoride and precipitation of 0.05% NaF solution on 6 groups of cow's tooth on which the artificial carious lesions were formed first. Test groups were calssified into two step application with NaF, $SnF_2$ and APF under different application time conditions plus 0.05% NaF solution(group I, II, III, IV) and single application with APF plus 0.05% NaF solution (group V) and control(0.05% NaF solution only, group IV). The obtained results were as follows. 1. Regarding microhardness change of enamel surface. microhardness increments in group I(NaF for 1 minute+APF for 3 minutes+0.05% NaF solution for 1 minute), II($SnF_2$ 1 min+APF 3 min+NaF sol.), III($SnF_2$ 2 min+APF 2 min+NaF sol.), IV(NaF 2 min+APF 2 min+NaF sol.) and V(APF 4 min+NaF sol.) were significantly greater than group VI.(P<0.05) 2. Microhardness changes of shorter application time of $SnF_2$ (group II) were significantly greater than group III. (P<0.05) 3. Microhardness changes were variable with kinds of fluoride, application sequence and application time of fluoride. but had no relation with the number of fluoride application.

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Handling Charactristics of FMT Net for the Larval Fish and Plankton (소형 어류 플랑크톤 채집용 FMT Net 의 운용 특성)

  • Jeong, Sun-Beom
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.41 no.2
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    • pp.122-128
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    • 2005
  • The experiments were conducted to fine out the handling characteristics of the FMT(Frame Mideater Trawl) in the southern waters of Korea using a trawler "DONGBAEK" belongs to Yosu National University. The realtionship between the net depth D(m) and the warp length L(m) at the towing speeds of 2.5k't and 3.5k't werw as follows ; D(m) = 0.30L - 1.3(2.5k't), D(m) = 0.16L - 1.5(3.5k't). Therefore, the net depth was 3.0m deeper when the warp length was 10m longer at the towing speed of 2.5k't and was 1.6m deeper for 10m longer at the speed of 3.5k't, respectively. The sinking speed of FMT was 6.5m/min when the warp releasing speed was 24m/min at the towing speed of 2.5k't and was 3.8m/min for 25m/min at the towing speed of 3.5k't, respectively. The rising speed of FMT was 6.9m/min when the warp rewinding speed was 28m/min at the towing speed of 2.5k't and was 5.3m/min for 25m/min at the towing speed of 3.5k't, respectively. The mean elapsed time getting to the stable towing condition was 104sec at the towing speed of 2.5k't and was 105sec at the towing speed of 3.5k't, respectively, and there was no time difference for the towing speed variation. During the towing, the net depth was comparatively stable on the condition of no change for the warp length and the towing speed.

Thermal Resistance Characteristics of Bacillus cereus, Escherichia coli O157:H7, and Listeria monocytogenes in a Multi-grain Soy Milk Product (레토르트 곡물 두유 내 Bacillus cereus, Escherichia coli O157:H7, Listeria monocytogenes의 내열특성)

  • Kim, Nam Hee;Koo, Jae Myung;Rhee, Min Suk
    • Korean Journal of Food Science and Technology
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    • v.47 no.5
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    • pp.593-598
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    • 2015
  • This study determined the thermal resistance of Bacillus cereus, Escherichia coli O157:H7, and Listeria monocytogenes in multi-grain soymilk and proposes processing conditions that meet the national standard for retort food products in Korea. D and z values were calculated from thermal inactivation kinetic curves after heating at 55, 60, and $65^{\circ}C$. The D value for B. cereus at $55^{\circ}C$ was the highest (22.8 min), followed by that for E. coli O157:H7 (18.8 min) and L. monocytogenes (17.6 min). At $60-65^{\circ}C$, the order was L. monocytogenes ($D_{60-65^{\circ}C}=3.4-0.9min$), E. coli O157:H7 (3.0-0.3 min), and B. cereus (1.2-0.3 min). The z values for these species were 5.2, 5.5, and $7.7^{\circ}C$, respectively. The Korean national standard for retort food products was achieved by thermal processing at $124{\pm}2^{\circ}C$ for 0.3-2.2 min. This study provides useful data for ensuring both the microbiological safety and product quality of multi-grain soymilk products.