• Journal of Pharmaceutical Investigation
• /
• 제25권3호
• /
• pp.205-211
• /
• 1995

To increase the solubility of iodine and iodine releasing agents, which are used widely as a topical broad spectrum antiseptics and disinfectant sanitizers, its inclusion complexes were prepared and studied. Inclusion complexes of iodine with ${\beta}-cyclodextrin$ were prepared by coprecipitation method and complex formation was acertained by differential scanning calorimetry and microscopic observation. Iodine content of inclusion complex was determined by means of iodometry. Tablets containing inclusion complex were manufactured with sugar, citric acid, magnesium stearate, dextrose. Stability of inclusion complexes and tablets was evaluated by accelerated stability test, and comparing with PVP-iodine. During preparation, use of 50% ethanol solution is preferable to water as the medium because the former resulted in more stable complex for a month under accelerated storage conditions. Solubility of iodine in KI aqueous solution was 0.048 g/ml and lower than in 50% ethanol solution. Inclusion complex and its tablets were very stable at severe condition for one month, and comparable to PVP-iodine in the aspect of stability. Inclusion complex tabletswere not affected with citric acid, sugar, dextrose, and direct tableting method was recommendable because wet granulation using ethanol gave some release of included iodine during process.

• 환경생물
• /
• 제38권3호
• /
• pp.424-432
• /
• 2020

The present study aimed to analyze the metaproteome of the microbial community comprising harmful algal bloom (HAB) in the Daechung reservoir, Korea. HAB samples located at GPS coordinates of 36°29'N latitude and 127°28'E longitude were harvested in October 2013. Microscopic observation of the HAB samples revealed red signals that were presumably caused by the autofluorescence of chlorophyll and phycocyanin in viable cyanobacteria. Metaproteomic analysis was performed by a gelbased shotgun proteomic method. Protein identification was conducted through a two-step analysis including a forward search strategy (FSS) (random search with the National Center for Biotechnology Information (NCBI), Cyanobase, and Phytozome), and a subsequent reverse search strategy (RSS) (additional Cyanobase search with a decoy database). The total number of proteins identified by the two-step analysis (FSS and RSS) was 1.8-fold higher than that by one-step analysis (FSS only). A total of 194 proteins were assigned to 12 cyanobacterial species (99 mol%) and one green algae species (1 mol%). Among the species identified, the toxic microcystin-producing Microcystis aeruginosa NIES-843 (62.3%) species was the most dominant. The largest functional category was proteins belonging to the energy category (39%), followed by metabolism (15%), and translation (12%). This study will be a good reference for monitoring ecological variations at the meta-protein level of aquatic microalgae for understanding HAB.

• 한국식품과학회지
• /
• 제21권4호
• /
• pp.549-555
• /
• 1989

저급육인 한우숫소의 앞다리고기를 도살직후 $16{\pm}2^{\circ}C$에서 고온숙성하여 연도개선 효과를 검토하였다. 또한 24시간 고온숙성한 후, 재구성육을 조제하여 결착성 개선방법을 모색하였다. 시료의 처리는 소금(0, 0.5, 1.0%), pyrophosphate(0, 0.3, 0.5%)와 이들의 혼용효과를 검토하였고, 소금(0.5%)과 pyrophosphate(0.3%)를 혼용한 원료에 succinic anhydride(0, 0.1, 0.2, 0.3%)를 첨가하여 석시닐산의 첨가효과를 검토하였다. 얻어진 결과는 다음과 같다. 1. 고온숙성은 저온숙성에 비하여 전단력이 낮았고, 근원섬유소편화지수가 높았으며, 연도개선의 효과가 뛰어났다. 전자현미경에 의한 형태학적 변화의 결과도 이들 결과 일치하였다. 2. 재구성육의 파단응력은 소금과 인산염의 첨가수준이 증가할수록 높아졌고, 소금과 인산염을 혼용할 경우, 현저히 증가하였다. 3. 조리수율은 소금과 인산염의 첨가수준이 증가할수록 낮아졌고, 소금과 인산염을 혼용할 경우, 현저하게 감소하였다. 4. TBA가는 소금 첨가수준에 비례하여 증가하였으며, 인산염 및 인산염과 소금혼용의 경우는 약간 증가하는 경향이었다. 5. 석신산은 재구성육의 결착력과 품질개선에 전혀 도움이 되지 않았다.

• Applied Biological Chemistry
• /
• 제6권
• /
• pp.33-36
• /
• 1965

(1) 한국중부산(韓國中部産) 광대싸리(Securinega suffruticosa Rohder)에 함유(含有)되는 식물성염기(植物性鹽基) Securinine의 추출(抽出)을 기도(企圖)하였다. (2) 한국원내식물분포도(寒國內源植物分布圖)는 극동지역(極東地域) 제일(第一)의 생태피도(生態被度)를 갖는 것으로 생각(生覺)된다. (3) 융점(融點)과 외부형태(外部形態)(현미경적(顯微鏡的)) 그리고 기타(其他)를 종합고찰(綜合考察)하여 추출물(抽出物)이 Securinine이라는 것을 확인(確認)하였다. (4) 수율(收率)을 평균(平均) 0.075% 였고 낙엽직전(落葉直前)의 황엽(黃葉)이란 점(點)이 함량(含量)을 감소(減少)케한 원인(原因)으로 생각(生覺)되었으며 하기생육(夏期生育)이 증가(增加)한 시기(時期)를 골라 재추출(再抽出)을 하면 상당(相當)한 수율(收率)의 증가(增加)를 가져올 것으로 추측(推測)되었다. (5) 수지상(樹脂狀) 점조물(粘稠物) 함유(含有) alkaloide 간(間)의 분리(分離)에는 새로운 용매(溶媒)의 선택(選擇)과 조작(操作)을 강구(講究)하여야 할것으로 생각(生覺)된다. (6) 추출과정(抽出過程)은 다소변경(多少變更)하였으며 앞으로 더욱 간편(簡便)하고 고수율(高收率)인 방법(方法)이 강구(講究)될 가능성(可能性)은 찾을수 있었다.

• 한국발생생물학회지:발생과생식
• /
• 제16권1호
• /
• pp.19-29
• /
• 2012

The ultrastructural characteristics of germ cell differentiations during spermatogenesis and mature sperm morphology in male $Atrina$ ($Servatrina$) $pectinata$ were evaluated via transmission electron microscopic observation. The accessory cells, which contained a large quantity of glycogen particles and lipid droplets in the cytoplasm, are assumed to be involved in nutrient supply for germ cell development. Morphologically, the sperm nucleus and acrosome of this species are ovoid and conical in shape, respectively. The acrosomal vesicle, which is formed by two kinds of electron-dense or lucent materials, appears from the base to the tip: a thick and slender elliptical line, which is composed of electron-dense opaque material, appears along the outer part (region) of the acrosomal vesicle from the base to the tip, whereas the inner part (region) of the acrosomal vesicle is composed of electron-lucent material in the acrosomal vesicle. Two special characteristics, which are found in the acrosomal vesicle of A. ($S$) $pectinata$ in Pinnidae (subclass Pteriomorphia), can be employed for phylogenetic and taxonomic analyses as a taxonomic key or a significant tool. The spermatozoa were approximately $45-50{\mu}m$ in length, including a sperm nucleus (about $1.43{\mu}m$ in length), an acrosome (about $0.51{\mu}m$ in length), and a tail flagellum (about $46-47{\mu}m$). The axoneme of the sperm tail evidences a 9+2 structure.

• KSBB Journal
• /
• 제22권4호
• /
• pp.239-243
• /
• 2007

본 연구에서는 미네랄오일에 유화제를 사용하여 한천 현탁액을 제조하고 여기에 피브로인을 첨가하여 마이크로캡슐을 제조하는 방법을 개발하였다. 먼저 한천 유화액에 첨가될 유화제의 농도를 10%로 결정하였고 피브로인을 투입하는 속도를 조절하여 피브로인으로 피막이 형성된 마이크로캡슐을 감압건조를 통하여 수분을 제거하고 에탄올 침전물로 회수하였다. 공초점 현미경으로 피브로인 피막이 형성되어 있음과 마이크로캡슐의 90%가 $1.32{\mu}m$에서 $6.0{\mu}m$사이에 존재함을 확인하였다. 에탄올 침전물 형태의 마이크로캡슐과 이들이 분산되는 것을 전자현미경으로 확인하였으며 열중량 분석을 통하여 마이크로캡슐은 중량비로 한천이 51.2%, 피브로인이 13.8%, Span 80이 1.4%로 구성되어있음을 확인하였다.

• Journal of Periodontal and Implant Science
• /
• 제36권3호
• /
• pp.705-716
• /
• 2006

The present study was performed to evaluate the effect of tetracycline-HCl on the change of implant surface microstructure according to application time. Implant with pure titanium machined surface, GBA surface and RBM surface were utilized. Implant surface was rubbed with 50mg/ml tetracycline-HCl solution for ${\frac{1}{2}}$min. 1min. $1{\frac{1}{2}}$min. 2min. and $2{\frac{1}{2}}$min. respectively in the test group. Then, specimens were processed for scanning electron microscopic observation. The results of this study were as follow. 1. Both test and control group showed a few shallow grooves and ridges in pure titanium machined surface implants. There were not significant differences between two group. 2. In GBA surfaces, control group exhibit many porous depression, and each depression were divided by strict border. Experimental group applied with tetracycline-HCl for 2min. were similar with control group. But when applied for $2{\frac{1}{2}}$min. surface alteration and border breakdown started, resulting enlargement of the porous depression. 3. In REM surface, control group exhibit rough, uneven surface with crater-like depression can be found. The surface alteration started when tetracycline-HCl was applied for 30sec. resulting breakdown of the crater-like depression. Depression became larger as applying time increased.

• Journal of Periodontal and Implant Science
• /
• 제36권3호
• /
• pp.717-729
• /
• 2006

The present study was performed to evaluate the effect of tetracycline-BCL on the change of implant surface microstructure according to application time. Implant with pure titanium machined surface, double coated $FBR^{(R)}$ surface and oxidized CellNest surface were utilized. Implant surface was rubbed with $50mg/m{\ell}$ tetracycline-BCL solution for ${\frac{1}{2}}$, 1, $1{\frac{1}{2}}$, 2 and $2{\frac{1}{2}}$min. respectively in the test group. Then, specimens were processed for scanning electron microscopic observation. The results of this study were as follows. 1. Both test and control group showed a few shallow grooves and ridges in pure titanium machined surface implants. There were not significant differences between two groups. 2. The double coated $FBR^{(R)}$ surfaces showed fine crystalline structures. The roughness of surfaces conditioned with tetracycline-BCL was lessened relative to the application time. 3. The oxidized CellNest surfaces showed the porous structures. The surface conditioning with tetracycline-BCI influenced on its micro-morphology. In conclusion, the detoxification of the affected implant surface with $50mg/m{\ell}$ tetracycline-BCL should be applied respectively with different time according to various implant surfaces.

• Journal of Periodontal and Implant Science
• /
• 제36권3호
• /
• pp.731-744
• /
• 2006

The present study was performed to evaluate the effect of tetracycline-HCl on the change of implant surface microstructure according to application time. Implant with pure titanium machined surface, HA-coated surface and dual acid etched surface were utilized. Implant surface was rubbed with $50mg/m{\ell}$ tetracycline-HCL solution for ${\frac{1}{2}}$min., 1min., $1{\frac{1}{2}}$min., 2min., and $2{\frac{1}{2}}$min. respectively in the test group. Then, specimens were processed for scanning electron microscopic observation. The results of this study were as follows. 1. Both test and control group showed a few shallow grooves and ridges in pure titanium machined surface implants. There were not significant differences between two groups. 2. In HA-coated surfaces, round particles were deposited irregularly. The roughness of surfaces conditioned with tetracycline-HCL was lessened and the cracks were increased relative to the application time. 3. The etched surfaces showed the honey comb structures. The surface conditioning with tetracycline-HCI didn't influence on its micro-morphology. In conclusion, the detoxification with $50mg/m{\ell}$ tetracycline-HCI must be applied respectively with different time according to various implant surfaces.

• 한국전기전자재료학회:학술대회논문집
• /
• 한국전기전자재료학회 2009년도 춘계학술대회 논문집
• /
• pp.1-2
• /
• 2009

The high critical current ($I_c$, A) of SmBCO coated conductor in a magnetic field, the high production rate and the high material yield are promising for applications. The inhomogeneity of Ie at the long length coated conduct is very important problem for electric application. So we researched the reason of inhomogeneity of $I_c$ at long length tape prepared by batch type co-evaporation system called by EDDC. The long length SmBCO coated conductors were developed on $LaMnO_3/IBAD-MgO/Y_2O_3/Al_2O_3$/Hastelloy C276 template. The distribution of $I_c$ are from 0 to 397 A/cm at 77 K and self field. We have studied the microstructures of these films by using SEM, EDS and X-ray diffraction. The XRD and composition by EDS results of SmBCO film reveals subtle difference. But, the microscopic observation by SEM show the microcrack at the sample with low $I_c$.