Development and Reproduction (한국발생생물학회지:발생과생식)

The Korean Society of Developmental Biology (한국발생생물학회)
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Germ Cell Differentiations during Spermatogenesis and Taxonomic Values of Mature Sperm Morphology of $Atrina$ ($Servatrina$) $pectinata$ (Bivalvia, Pteriomorphia, Pinnidae)

  • Kang, Hee-Woong (West Sea Fisheries Research Institute, National Fisheries Research & Development Institute) ;
  • Chung, Ee-Yung (Dept. of Marine Biotechnology, Kunsan National University) ;
  • Kim, Jin-Hee (Marine Eco-Technology Institute) ;
  • Chung, Jae-Seung (Dept. of Urology, College of Medicine, Inje University) ;
  • Lee, Ki-Young (Dept. of Marine Biotechnology, Kunsan National University)
  • Received : 2012.01.02
  • Accepted : 2012.01.20
  • Published : 2012.03.31
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Abstract

The ultrastructural characteristics of germ cell differentiations during spermatogenesis and mature sperm morphology in male $Atrina$ ($Servatrina$) $pectinata$ were evaluated via transmission electron microscopic observation. The accessory cells, which contained a large quantity of glycogen particles and lipid droplets in the cytoplasm, are assumed to be involved in nutrient supply for germ cell development. Morphologically, the sperm nucleus and acrosome of this species are ovoid and conical in shape, respectively. The acrosomal vesicle, which is formed by two kinds of electron-dense or lucent materials, appears from the base to the tip: a thick and slender elliptical line, which is composed of electron-dense opaque material, appears along the outer part (region) of the acrosomal vesicle from the base to the tip, whereas the inner part (region) of the acrosomal vesicle is composed of electron-lucent material in the acrosomal vesicle. Two special characteristics, which are found in the acrosomal vesicle of A. ($S$) $pectinata$ in Pinnidae (subclass Pteriomorphia), can be employed for phylogenetic and taxonomic analyses as a taxonomic key or a significant tool. The spermatozoa were approximately $45-50{\mu}m$ in length, including a sperm nucleus (about $1.43{\mu}m$ in length), an acrosome (about $0.51{\mu}m$ in length), and a tail flagellum (about $46-47{\mu}m$). The axoneme of the sperm tail evidences a 9+2 structure.

Keywords

References

  1. Baccetti B, Afzelius BA (1976) Biology of sperm cell. S. Krager, New York. Monogr Devel Biol 10:1-254.
  2. Baccetti B (1979) The evolution of the acrosomal complex. In: Fawcett DW., Bedford JM (eds.) The Spermatozoon. Urban Schwarzenberg, Baltimore, pp 305-328.
  3. Bernard RTF, Hodgson AN (1985) The fine structure of the sperm and spermatid differentiation in the brown mussel Pernaperna. South Africa J Zool 20:5-9. https://doi.org/10.1080/02541858.1985.11447904
  4. Chung EY, Ryou DK (2000) Gametogenesis and sexual maturation of the surf clam, Mactra veneriformis on the west coast of Korea. Malacologia 42:149-163.
  5. Chung EY, Park KY, Son PW (2005) Ultrastructural study on spermatogenesis and sexual maturation of the mail jicon scallop, Chlamys farreri on the west coast of Korea. Kor J Malacol 21:95-105.
  6. Chung EY, Kim EJ, Park GM (2007) Spermatogenesis and sexual maturation in male Mactra chinensis (Bivalvia: Mactridae) of Korea. Integr Bios 11:227-234. https://doi.org/10.1080/17386357.2007.9647339
  7. Chung EY, Chung CH, Kim JH, Park SW, Park KH (2010) Ultrastructures of germ cells and the accessory cells during spermatogenesis in male Gomphina veneriformis (Bivalvia: Veneridae) on the east coast of Korea. Kor J Malacol 26:51-62.
  8. Chung EY, Kim JH, Kim SH, Seo WJ (2011) Germ cell development during spermatogenesis and some characteristics of mature sperm morphology in male Scapharca subcrenata (Pteriomorphia: Arcidae) in Western Korea. Kor J Malacol 27:121-129. https://doi.org/10.9710/kjm.2011.27.2.121
  9. Daniels EW, Longwell AC, McNiff JM, Wolfgang RW (1971) A reinvestigation of the ultrastructure of the spermatozoa from the American oyster Crassostrea virginica. Trans American Microscopy Society. 90:275-282. https://doi.org/10.2307/3225187
  10. Dorange G, Le Pennec M (1989) Ultrastructural characteristics of spermatogenesis in Pecten maximus (Mollusca, Bivalvia). Inver Reprod & Develop 15:109-117. https://doi.org/10.1080/07924259.1989.9672031
  11. Drozdov TA, Reunov AA (1986) Spermatogenesis and the sperm ultrastructure in the mussel Modiolus difficillis. Tsitologiia 28:1069-1074.
  12. Eckelbarger KJ, Bieler R, Mikkelsen PM (1990) Ultrastructure of sperm development and mature sperm morphology in three species of commensal bivalves (Mollusca: Galeommatoidea). J Morph 205:63-75. https://doi.org/10.1002/jmor.1052050107
  13. Eckelbarger KJ, Davis CV (1996) Ultrastructure of the gonad and gametogenesis in the eastern oyster, Crassostrea virginica. II. Testis and spermatogenesis. Mar Biol 127: 89-96. https://doi.org/10.1007/BF00993648
  14. Franzen A (1956) On spermatogenesis, morphology of the spermatozoon, and biology of fertilization among invertebrates. Zoology of Bidr Uppsala 31:355-482.
  15. Franzen A (1970) Phylogenetic aspects of the mophology spermatozoa and spermiogenesis in Baccetti B (ed): Comparative Spermatology. Accademia Nationale Dei Lincei, Rome pp 1-573.
  16. Franzén Å (1983) Ultrastructural studies of spermatozoa in three bivalve species with notes on evolution of elongated sperm nucleus in primitive spermatozoa. Gamete Research 7:199-214. https://doi.org/10.1002/mrd.1120070302
  17. Gaulejac de J, Jenry M, Vicente N (1995) An ultrastructural study of gametogenesis of the marine bivalve Pinna nobilis (Linnaeus, 1758). II. Spermatogenesis. Journal of Molluscan Study 61:393-403. https://doi.org/10.1093/mollus/61.3.393
  18. Healy JM (1989) Spermiogenesis and spermatozoa in the relict bivalve genus Neotrigonia: relevance to trigonioid relationships, particularly Unionoidea. Mar Biol 103:75-85. https://doi.org/10.1007/BF00391066
  19. Healy JM, Lester RJG (1991) Sperm ultrastructure in the Australian oyster Saccostrea commercialis (Iredale and Roughley) (Bivalvia: Ostreidea). Journal of Molluscan Studies 57:219-224. https://doi.org/10.1093/mollus/57.2.219
  20. Healy JM (1995) Sperm ultrastructure in in the marine bivalve families Carditidae and Crassatellidae and its bearing on unification of the Crasssatelloidea with the Carditoidea. Zool Sci 24:21-28. https://doi.org/10.1111/j.1463-6409.1995.tb00472.x
  21. Hodgson AN, Bernard RTF (1986) Ultrastructure of the sperm and spermatogenesis of three species of Mytilidae (Mollusca, Bivalvia). Gamete Res 15:123-135. https://doi.org/10.1002/mrd.1120150204
  22. Jamieson BGM (1987) The Ultrastructure and Phylogeny of Insect Spermatozoa. Cambridge University Press, Cambridge.
  23. Jamieson BGM (1991) Fish Evolution and Systematics: Evidence from Spermatozoa. Cambridge University Press, Cambridge pp 181-194.
  24. Kim JH (2001) Spermatogenesis and comparative ultrastructure of spermatozoa in several species of Korean economic bivalves (13 families, 34 species). Ph.D. thesis, Pukyung National University, pp 1-161.
  25. Kim JH, Chung EY, Choi KH, Lee KY, Choi MS (2010a) Ultrastructure of the testis and germ cell development during spermatogenesis in male Crassostrea gigas (Bivalvia: Ostreidae) in western Korea. Kor J Malacol 26:235-244.
  26. Kim JH, Chung EY, Lee KY, Choi MS, Kim SH (2010b) Spermatid differentiations during and mature sperm ultrastructure in male Crassostrea niponica (Saki, 1934) (Pteriormorphia: Ostreidae). Kor J Malacol 26:311-316.
  27. Kim JH, Chung EY, Choi KH, Park KH, Park SW (2010c) Ultrastructure of germ cells during spermatogenesis and some characteristics of sperm morphology in male Mytilus coruscus (Bivalvia: Mitilidae) on the west coast of Korea. Kor J Malacol 26:33-43.
  28. Kim JH, Kim SH (2011) Germ cell development during spermatogenesis and taxonomic values of sperm morphology in Septifer (Mytilisepta) virgatus (Bivalvia: Mitilidae). Dev Reprod 15:239-247.
  29. Longo FJ, Dornfeld EJ (1967) The fine structure of spermatid differentiation in the mussel Mytilus edulis. J Ultrastruct Res 20:462-480. https://doi.org/10.1016/S0022-5320(67)80113-8
  30. Longo FJ, Anderson E (1969) Spermatogenesis in the surf clam Spisular solidissima with special reference to the formation of the acrosomal vesicle. J Ultrastruct Res 27:435-443. https://doi.org/10.1016/S0022-5320(69)80042-0
  31. Popham JD (1979) Comparative spermatozoon morphology and bivalve phylogeny. Malacol Rev 12:1-20.
  32. Sousa M, Corral L, Azevedo C (1989) Ultrastructural and cytochemical study of spermatogenesis in Scrobicula riaplana (Mollusca, Bivalvia). Gamete Res 24:393-401. https://doi.org/10.1002/mrd.1120240406
  33. Verdonk NH, Van Den Biggelaar JAM, Tompa AS (1983) The Mollusca. Vol. 3. Development. Academic press. New York, pp 1-48.