• Title/Summary/Keyword: Microscope

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Error Analysis and Alignment Tolerancing for Confocal Scanning Microscope using Monte Carlo Method (Monte Carlo 방법을 이용한 공초점 주사 현미경의 오차 분석과 정렬 공차 할당에 관한 연구)

  • 유홍기;강동균;이승우;권대갑
    • Journal of the Korean Society for Precision Engineering
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    • v.21 no.2
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    • pp.92-99
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    • 2004
  • The errors can cause the serious loss of the performance of a precision machine system. In this paper, we proposed the method of allocating the alignment tolerances of the parts and applied this method to get the optimal tolerances of a Confocal Scanning Microscope. In general, tight tolerances are required to maintain the performance of a system, but a high cost of manufacturing and assembling is required to preserve the tight tolerances. The purpose of allocating the optimal tolerances is minimizing the cost while keeping the high performance of the system. In the optimal problem, we maximized the tolerances while maintaining the performance requirements. The Monte Carlo Method, a statistical simulation method, is used in tolerance analysis. Alignment tolerances of optical components of the confocal scanning microscope are optimized to minimize the cost and to maintain the observation performance of the microscope. We can also apply this method to the other precision machine system.

The Optical Design of Miniaturized Microscope Objective for CARS Imaging Catheter with Fiber Bundle

  • Rim, Cheon-Seog
    • Journal of the Optical Society of Korea
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    • v.14 no.4
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    • pp.424-430
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    • 2010
  • In coherent anti-Stokes Raman scattering (CARS) microscopy reported until now, conventional microscope objectives are used, so that they are limited for introduction into a living body. Gradient-index (GRIN) rod lenses might be a solution for miniaturized microscope objectives for in-vivo CARS microscopy. However, due to the inherent large amount of chromatic aberration, GRIN rod lenses cannot be utilized for this purpose. CARS imaging catheter, composed of miniaturized microscope objective and fiber bundle, can be introduced into a living body for minimally invasive diagnosis. In order to design the catheter, we have to first investigate design requirements. And then, the optical design is processed with design strategies and intensive computing power to achieve the design requirements. We report the miniaturized objective lens system with diffraction-limited performance and completely corrected chromatic aberrations for an in-vivo CARS imaging catheter.

Shear Induced Homogenization Study of an Immiscible Polymer Blend Solution Using Fluorescence Microscope (형광 현미경을 이용한 비상용 고분자 블렌드 용액의 전단 균질화 연구)

  • 유재웅
    • Polymer(Korea)
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    • v.27 no.5
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    • pp.413-420
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    • 2003
  • Fluorescence microscope technique was employed for the characterization of phase separation behavior of a 4-chloro-7-nitrobenzofurazan labeled polystyrene (PS) / polybutadiene (PB) blend in dioctyl phthalate under steady shear. It was confirmed that the fluorescence microscope images reflect the real phase morphology by comparing with the images of phase contrast microscope. Comparing the fluorescence intensities from the phase separated domain (PS rich) and continuous phase (PB rich), the composition difference between these two phases were deduced. The observed shear dependence of compositional change is then used to confirm that the phase diagram is indeed shifted under the steady shear.

Design and Manufacture of an Electron Detector for Scanning Electron Microscope (주사전자현미경용 전자검출기의 설계 및 제작)

  • Jeon, Jong-Up;Kim, Ji-Won
    • Journal of the Korean Society for Precision Engineering
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    • v.25 no.4
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    • pp.53-60
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    • 2008
  • Electron detectors used in scanning electron microscope accept electrons emitted from the specimen and convert them to an electrical signal that, after amplification, is used to modulate the gray-level intensities on a cathode ray tube, producing an image of the specimen. Electron detector is one of the key components dominating the performance of scanning electron microscope so that the development of electron detectors having high performance is indispensable to acquire high quality images using scanning electron microscope. In this paper, we designed and manufactured an electron detector and conducted a couple of image capture experiments using it. In particular, scintillator which generates light photons when it is struck by high-energy electrons was manufactured and experimental studies on the optimization of manufacturing condition was carried out. From experiments to evaluate the performance of our detector, it was verified that the performance of our detector is equivalent to or better than that of the conventional one.

Use of Stereoscopic Microscope for Practice of Microsurgical Skill (미세수술 술기 연습을 위한 실체 현미경의 사용)

  • Song, Jeong-Hoon;Jeong, Eui-Cheol;Kwon, Sung-Tack
    • Archives of Reconstructive Microsurgery
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    • v.18 no.2
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    • pp.75-78
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    • 2009
  • Purpose: Microsurgery is an essential technique in plastic and reconstructive surgery. However, microsurgical suturing and handling of the instrument are difficult for beginners, and who requires a steep learning curve. Therefore, methods for improving the technical skill are needed. The authors describe the value of a small stereoscopic microscope as a training tool. Materials and Methods: A small stereoscopic microscope was used to help improve the microsurgical skill. Monofilament 10-0 Nylon and a surgical rubber globe were used as a suture material and education material, respectively Result: Stereoscopic view of the operation field was obtained and basic microsurgical suture was possible. Conclusion: The stereoscopic microscope is an effective training tool for beginners of microsurgery with benefits in cost and usefulness in small place.

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Theoretical Study of Scanning Probe Microscope Images of VTe2

  • Park, Sung-Soo;Lee, Jee-Young;Lee, Wang-Ro;Lee, Kee-Hag
    • Bulletin of the Korean Chemical Society
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    • v.28 no.1
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    • pp.81-84
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    • 2007
  • Ab initio periodic Hartree-Fock calculations with the full potential and minimum basis set are applied to interpretation of scanning tunneling microscope (STM) and atomic force microscope (AFM) images on 1TVTe2. Our results show that the simulated STM image shows asymmetry while the simulated AFM image shows the circular electron densities at the bright spots without asymmetry of electron density to agree with the experimental AFM image. The bright spots of both the STM and AFM images of VTe2 are associated with the surface Te atoms, while the patterns of bright spots of STM and AFM images are different.

Investigation of the Frictional Behavior with respect to Surface Geometry and Surface Material at Nanoscale (나노스케일에서의 표면형상 및 재료변화에 대한 마찰거동 고찰)

  • 성인하;김대은
    • Proceedings of the Korean Society of Tribologists and Lubrication Engineers Conference
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    • 2001.06a
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    • pp.36-41
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    • 2001
  • In this work, the changes in the friction force(lateral force) with respect to nanoscale geometric variation were investigated using an Atomic Force Microscope and a Lateral Force Microscope. It could be concluded that the changes in the friction force correspond well to the slope change rather than the surface slope itself, and that the influence of slope change on the frictional behavior is dependent on the magnitude of the slope and the torsional stiffness of the cantilever. Also, the nominal friction force is found to be more significantly affected by the material and the physical-chemical state of the surface rather than by nanoscale geometric steps. However, the change in nanoscale geometric details of the surface cause instantaneous change and slight variation in the friction signal.

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Scanning confocal microscope using a quad-detector (4분할 photodiode를 이용한 scanning confocal microscope)

  • 유석진;김수철;이진서;권남익
    • Korean Journal of Optics and Photonics
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    • v.8 no.2
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    • pp.165-168
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    • 1997
  • We have constructed a scanning confocal microscope using a 780 nm semiconductor laser, an actuator of a compact disk player and a quad-detector. This device detects heights and characteristics of a surface. The laser focus was located at the surface of a sample by using the error signal obtained by a quad-dector, and the current supplied to the actuator for lens was displayed as a height. The materials of a surface were classified according to reflected total intensities and was displayed by different color in a monitor. The device has very samll dimensions of 30 mm$\times$20 mm$\times$20 mm and scan field is 1.6 mm$\times$1.6mm. We obtained two images, one using only reflected light and the other using an error signal from a quad-detector and compared these two images.

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Precision measurement of a laser micro-processing surface using a hybrid type of AFM/SCM (하이브리드형 AFM/SCM을 이용한 레이저 미세 가공 표면 측정)

  • Kim, Jong-Bae;Kim, Kyeong-Ho;Bae, Han-Sung;Nam, Gi-Jung;Lee, Dae-Chul;Seo, Woon-Hak
    • Proceedings of the Korean Society of Laser Processing Conference
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    • 2006.11a
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    • pp.123-127
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    • 2006
  • Hybrid type microscope with a Scanning Confocal Microscope (SCM) and a shear-force Atomic Force Microscope (AFM) is suggested and preliminarily studied. A image of $120{\times}120{\mu}m^2$ is obtained within 1 second by SCM because scan speed of a X-axis and Y-axis are 1kHz and 1Hz, respectively. Shear-force AFM is able to correctly measure the hight and width of sample with a resolution 8nm. However, the scan speed is slow and it is difficult to distinguish a surface composed of different kinds of materials. We have carried out the measurement of total image of a sample by SCM and an exact analysis of each image by shear-force AFM.

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Three-Dimensional Video Microscopy: Potential for Improved Ergonomics without Increased Operative Time?

  • Yasmina Samaha;Edward Ray
    • Archives of Plastic Surgery
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    • v.50 no.1
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    • pp.125-129
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    • 2023
  • Three-dimensional (3D) video exoscopes are high-magnification stereo cameras that project onto monitors mounted in the operating room, viewable from different angles. Outside of plastic surgery, exoscopes have been shown to successfully improve the ergonomics of microsurgery, though sometimes with prolonged operating times. We compare a single surgeon's early experience performing free flap procedures from 2020 to 2021 using either a binocular microscope or a 3D video exoscope. Ten procedures were performed with the standard operating microscope and 8 procedures with the 3D exoscope. The microsurgeon, having minimal prior experience using an exoscope, reported less neck discomfort following the free flap procedures performed with the exoscope compared with the binocular surgical microscope. Total average operating time was comparable between the standard surgical microscope and the 3D exoscope (13.7 vs. 13.4 hours, p = 0.34). Our early experience using a 3D exoscope in place of a standard optical microscope demonstrated that the exoscope shows promise, offering an ergonomic alternative during microvascular reconstruction without increasing overall operating times. Future studies will compare free flap ischemia time between cases performed using the exoscope and the conventional binocular microscope. Medical Subject Headings authorized following words: free tissue flaps; operating rooms; ergonomics; microsurgery.