• Title/Summary/Keyword: Microbiological evaluation

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Posttraumatic Intracranial Tuberculous Subdural Empyema in a Patient with Skull Fracture

  • Kim, Jiha;Kim, Choonghyo;Ryu, Young-Joon;Lee, Seung Jin
    • Journal of Korean Neurosurgical Society
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    • v.59 no.3
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    • pp.310-313
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    • 2016
  • Intracranial tuberculous subdural empyema (ITSE) is extremely rare. To our knowledge, only four cases of microbiologically confirmed ITSE have been reported in the English literature to date. Most cases have arisen in patients with pulmonary tuberculosis regardless of trauma. A 46-year-old man presented to the emergency department after a fall. On arrival, he complained of pain in his head, face, chest and left arm. He was alert and oriented. An initial neurological examination was normal. Radiologic evaluation revealed multiple fractures of his skull, ribs, left scapula and radius. Though he had suffered extensive skull fractures of his cranium, maxilla, zygoma and orbital wall, the sustained cerebral contusion and hemorrhage were mild. Eighteen days later, he suddenly experienced a general tonic-clonic seizure. Radiologic evaluation revealed a subdural empyema in the left occipital area that was not present on admission. We performed a craniotomy, and the empyema was completely removed. Microbiological examination identified Mycobacterium tuberculosis (M.tuberculosis). After eighteen months of anti-tuberculous treatment, the empyema disappeared completely. This case demonstrates that tuberculosis can induce empyema in patients with skull fractures. Thus, we recommend that M. tuberculosis should be considered as the probable pathogen in cases with posttraumatic empyema.

Storge Stability of Barleys Irradiated by Gamma-Ray (감마선 조사에 보리의 저장 안전성)

  • 김미라;손인숙
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.28 no.5
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    • pp.1076-1081
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    • 1999
  • Physicochemical, microbiological, and sensory properties of barleys irradiated by gamma ray at 1.2kGy, 10.1kGy, or 30.5kGy were investigated every 40 days during the storage at 25℃ and 50% relative humidity. Moisture content of the irradiated barleys decreased but crude lipid content increased during the storage. TBA values increased in proportion to the irradiation dose and to the storage period. In Hunter's color, L, a, and b values of 30.5kGy dose irradiated barleys were higher than those of the non irradiated barleys right after irradiation and this trend continued during the storage. Numbers of mesophilic and psychrophilic bacteria in the non irradiated barleys and 1.2kGy dose irradiated barleys were higher than those in the barleys irradiated at 10.1kGy and 30.5kGy during the storage. Numbers of yeasts and molds in the irradiated and non irradiated barleys were low and they did not greatly increase during the storage. In sensory evaluation, acidic odor of the barleys was strong at the 10.1kGy and 30.5 kGy dose irradiation but barley odor and humid odor were not significantly different among the groups depending upon the radiation dose and storage period.

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A Comparative Study on Correlation Through Physiochemical Property Comparision of Livestock Liquid Fertilizer (가축분뇨 액비의 이화학적 특성비교를 통한 상관성 비교연구)

  • Jeon, Sang-Joon;Kim, Soo-Ryang;Hong, In-Gi;Kim, Ha-Je;Kim, Dong-Gyun;Lee, Myung-Gyu
    • Journal of Animal Environmental Science
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    • v.19 no.2
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    • pp.163-168
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    • 2013
  • Today, a desirable way to manage livestock manure is to activate its utilization as a resource. The production of high quality liquid fertilizer of livestock manure is very important because it increases the use of various liquid fertilizer. However, the result of mature evaluation with a maturity measuring instrument for liquid fertilizer showed that the deviation of concentration between liquid fertilizer did not bring into uniformity. The result is also making sure that quality management for liquid fertilizer is not smoothly made. Quality evaluation for compost and liquid fertilizer includes physical, biological, chemical and microbiological methods, but a chemical method is mainly being implemented due to fairness and field application. Therefore, this study figured out correlation in feces and urine through regression analysis of livestock manure and tried to create a research plan to carry out efficient quality analysis of managing livestock manure.

A study on the Development of Standardized Recipe and the Microbiological Assessment and Sensory Evaluation of Various Bulkogis for Steam Convection Oven and Cook/Chill System for Kindergarten Foodservice Operations (유치원 급식에 적용하기 위한 불고기류의 Steam Convection Oven 및 Cook/Chill System용 레시피 개발 및 미생물적, 관능적 품질 평가에 대한 연구)

  • 강현주;김경자;김은희
    • Korean journal of food and cookery science
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    • v.14 no.4
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    • pp.358-366
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    • 1998
  • To meet the demand for high quality of foods with the expanded implementation of foodservice into kindergarten, and to make the efficient use of resources, the necessity of introducing central production unit with cook/chill system into kindergarten foodservice is getting increased. The purpose of this study were to develop standardized recipe applicable to cook/chill system for kindergarten foodservice and to evaluate the microbial and sensory quality. For quantitty production of cook/chill system in kindergarten foodservice, Various Bulkogis were selected as menu items. The followings are summary of the results: 1. Standard recipes to produce Various Bulkogis(Broiled Sliced Meat with Sauce) for cook/chill system have been developed. 2. The microbial quality of Various Bulkogis during 10 days chilled storage was very good. And in this experiment, the stability of storage of standard recipes of Various Bulkogis at cook/chill system was proved for 10 days. 3. All of the items of sensory evaluation of Bulkogis has no significance between the first day and the third day of storage, except the color of Pork Bulkogi (P<0.05).

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Evaluation of the Filtration Efficiency and Facial Inhalation Resistance of Various Commercial Masks (시중에 판매되는 다양한 비인증 마스크의 분진 포집효율과 안면부 흡기저항 평가)

  • Kang, Sohyun;Kim, Soomin;Yoon, Chung Sik;Lee, Kiyoung
    • Journal of Environmental Health Sciences
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    • v.47 no.3
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    • pp.292-301
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    • 2021
  • Objectives: Wearing medical masks has been recommended since the declaration of coronavirus disease-19 (COVID-19) as a pandemic disease. Certified medical masks are evaluated according to filtration efficiency and facial inhalation resistance. However, some people use non-certified common masks. This study aimed to evaluate various non-certified commercial masks based on the certification criteria for medical masks. Methods: Twenty mask products (three anti-droplet, three disposable dental, eight fashion, three cotton, and three children's masks) were selected. For performance evaluation, filtration efficiency and facial inhalation resistance tests were conducted. The evaluation method followed the certification method for KF-certified masks of the Ministry of Food and Drug Safety (MFDS) and the N95 respirator of the National Institute for Occupational Safety and Health (NIOSH). Results: None of the 20 masks met the KF94 certification standard set by the MFDS. Four and three masks respectively met the KF80 certification standard and the N95 standard of NIOSH. Filtration efficiency was significantly higher in three-layer masks than in single layer masks. Pleated-type masks had higher filtration efficiency than cone-type masks. There was no correlation between the structure of masks and facial inhalation resistance. Conclusion: While no masks complied with the KF94 certification standard, a few masks met the KF80 and the N95 certification standards of NIOSH. Although some people wear non-certified commercial masks, protection from aerosols is not guaranteed by such masks. Evaluation of the protection efficiency of non-certified mask against microbiological infection is needed for the prevention of infectious disease.

Microbiological and Genotoxicological Safety of Gamma-Irradiated Chicken (감마선조사 닭고기의 미생물학적 및 유전독성학적 안전성 평가)

  • 곽희진;정차권;강일준
    • Korean journal of food and cookery science
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    • v.17 no.6
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    • pp.617-624
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    • 2001
  • Gamma irradiation (1-10 kGy) was applied to chicken for the evaluation of their microbiological safety and possible genotoxicity. In 3 kGy-irradiated sample, the growth of psychrophile was inhibited about 1.5 log cycles and no cells were recovered in total microbial counts. All kinds of contaminated microorganism were sterilized by 7 kGy-irradiation. Also, irradiation followed by freeze-storage at the same time was very effective in inhibiting bacterial growth. The genotoxicity of 10 kGy-irradiated chicken was evaluated by Salmonella Typhimurium reversion assay and in vivo micronucleus assay using mouse bone marrow cells. The results were negative in the bacterial reversion assay with S. Typhimurium TA98, TA100, TA1535, and TA1539. Clastogenic effects were not shown in vivo mouse micronucleus assay at 10 kGy-dose tested.

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Establishment of Hygienic Standards for Pizza Restaurant Based on HACCP Concept -Focused on Pizza Production- (HACCP의 적용을 위한 피자 전문 레스토랑의 위생관리 기준 설정 -피자생산을 중심으로-)

  • Lee, Bog-Hieu;Huh, Kyoung-Sook;Kim, In-Ho
    • Korean Journal of Food Science and Technology
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    • v.36 no.1
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    • pp.174-182
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    • 2004
  • Hygienic standards for pizza specialty restaurant located in Seoul during summer, 2000 were established based on HACCP concept by measuring temperature, time, pH, $A_{w}$ and microbiological assessments of pizza, and evaluation of hygienic conditions of kitchens and workers. Kitchen and worker conditions were average 1.2 and 1.0 (3 point Sly's scale), respectively, Microbial contaminations occurred at $5-60^{\circ}C$, pH above 5.0, and $A_{w}$ (0.93-0.98). Microbial assessments for pizza processing revealed $1.5{\times}10^{2}-3.9{\times}10^{8}\;CFU/g$ of TPC and $0.5{\times}10^{1}-1.6{\times}10^{7}\;CFU/g$ of coliforms, exceeding standards ($TPC\;10^{6}\;CFU/g\;and\;coliform\;10^{3}\;CFU/g$) established by Solberg et al., although significantly decreased after baking. S. aureus was not discovered, but Salmonella was found in onions. Tools and containers such as pizza cutting knife, topping container, serving bowl, pizza plate, working board, and dough kneading board contained $6.2{\times}10^{2}-1.1{\times}10^{9}\;CFU/g$ of TPC, $2.0{\times}10^{1}-6.2{\times}10^{3}\;CFU/g$ of coliforms. Workers' hands contained $3.1{\times}10^{4}\;CFU/g$ of TPC and S. aureus as compared to safety standards of Harrigan and McCance (500 and 10 CFU/g of TPC and coliforms per $100cm^{2}$). CCPs (critical control points) were determined as receiving, topping, and baking according to CCP decision tree analysis. Results suggest purchase of quality materials, careful monitoring of time and temperature, hygienic use of tools and utensils, and sanitary practicer by workers are recommended as control points for safe pizza production.

Quality Characteristics of Gamma Irradiated-Imported Orange during Storage at Room Temperature (20℃) (감마선 조사 수입 오렌지의 상온저장(20℃) 중 품질 특성)

  • Kyung, Eun-Ji;Kim, Kyoung-Hee;Yook, Hong-Sun
    • The Korean Journal of Food And Nutrition
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    • v.27 no.2
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    • pp.183-193
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    • 2014
  • This study is conducted to evaluate the effects of gamma irradiation (0.4, 0.6, 0.8, 1 and 1.5 kGy) on the microbiological, physicochemical and sensory qualities of imported oranges during storage at $20^{\circ}C$ for 15 days. Total aerobic bacteria and yeast/mold counts in non-irradiated oranges increase significantly with increasing storage time. Irradiation has effects on the reduction of microorganism of dose-dependent oranges. The vitamin C contents decrease significantly according to dose-dependent manners and storage times after the gamma irradiation. Sensory evaluation decreases according to dose-ependent manners and storage times, excluding the color. The results suggest that gamma irradiation is effective for ensuring the microbiological safety, but the irradiated oranges more than 1 kGy are not good for physicochemical and sensory qualities. Therefore, irradiated samples of 0.4~0.6 kGy are considered as the optimum-dose for maintaining quality.

Quality Evaluation of Soybean soup by Adding Gwackhyang in Foodservice Operations (단체급식을 위한 콩국의 곽향 첨가에 따른 품질평가)

  • Ko, Seong Hee;Lee, Kyung Yeoun
    • Korean journal of food and cookery science
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    • v.29 no.6
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    • pp.841-846
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    • 2013
  • Developing a menu for foodservice operations utilizing a Yaksun meets with the desire of customers who have keen interest in health food. In addition, it is possible to expect the additional advantage of decreasing hygiene risk, which can be caused by large quantity cooking, by the efficacy of medicinal herbs used in Yaksun. Accordingly, this study provides with basic information for the development of a Yaksun menu for foodservice operations utilizing Gwackhyang by evaluating the sensory quality and microbiological quality of bean soup added of Gwackhyang. The standard plate counting significantly decreased as the addition quantity of Gwackhyang increased (p<0.001). All bean soups, which were kept at room temperature and refrigerator for 3 hours after cooking, met the safety standard 5.00 CFU/g. The number of coliforms also significantly decreased as the addition quantity of Gwackhyang increased. The bean soups in control group exceeded the safety standard of 2.00 CFU/g after 1 hour at room temperature and in refrigerator. On the other hand, all Gwackhyang bean soups met safety standard, only excluding G1 with 1% Gwackhyang kept at room temperature. Accordingly, it has been confirmed that the addition of Gwackhyang during bean soup production decreases the microorganism level of bean soup. Both standard plate counting and the number of coliforms significantly increased after 3 days in refrigerator; however, the increasing extent of Gwackhyang bean soups were less than the control group bean soups. The control group received scored highest in 3 sensory characteristics out of 5 sensory characteristics. However, G1 with 1% Gwackhyang also scored highest in 2 sensory characteristics, which were taste and overall preference. G2 also scored 4.45 in color and 4.15 in fragrance, which were lower than the control group but were above average.

Effects of Different Marination Conditions on Quality, Microbiological Properties, and Sensory Characteristics of Pork Ham Cooked by the Sous-vide Method

  • Jeong, Kiyoung;O, Hyeonbin;Shin, So Yeon;Kim, Young-Soon
    • Food Science of Animal Resources
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    • v.38 no.3
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    • pp.506-514
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    • 2018
  • The aim of this study was to evaluate the effects of marinade under different conditions (temperature and vacuum) on pork ham cooked by the sous-vide method ($61^{\circ}C$ and 98.81% vacuum for 45 min). Control group was non-marinade pork ham. The samples were marinated under 1 of 4 conditions: $4^{\circ}C$, 98.81% vacuum (treatment group $T_1$); $4^{\circ}C$, atmospheric pressure ($T_2$); $20^{\circ}C$, 98.81% vacuum ($T_3$); and $20^{\circ}C$, atmospheric pressure ($T_4$). The pH value was higher in the control (6.02) than in the treatment groups (4.30-4.42, p<0.001). Shear force was the lowest in the control: 18.14 N. Lightness and redness values were higher in the control (p<0.001). The chroma value significantly decreased from 12.74 to 7.55 with marinade (p<0.001). Total viable and coliform counts of raw meat were 84.6 and 3.67 Log CFU/g, respectively. After the marinade, the total viable count decreased to 3.00-14.67 Log CFU/g (p<0.001). Coliforms were not detected. After sous-vide cooking, no viable microorganisms were detected in any group. Treatment groups generally showed high scores on consumer preference. The marinade and sous-vide cooking had a positive effect on sensory characteristics. They provided safe conditions for sanitary evaluation. As a result, it appears that marinade at refrigeration temperature is better than that at room temperature.