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Seroprevalence of antibodies to Neospora caninum in cattle at Seoul slaughtering center (농협 서울축산물공판장 도축우에서의 Neospora caninum 혈청항체 양성률 조사)

  • Kim, Jee-Eun;Son, Jang-Won;Yang, Yun-Mo;Jeon, Hae-Chang;Jin, Kyung-Sun;Kim, Kyu-Hyun;Shin, Bang-Woo;Lee, Jung-Hark
    • Korean Journal of Veterinary Service
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    • v.34 no.2
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    • pp.179-185
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    • 2011
  • Neospora caninum is now recognized as one of the important causes of abortion in cattle worldwide, but there was no report on seroprevalence of antibodies to N. caninum in cattle at Seoul slaughtering center. Serum samples from 610 Korean indigenous cattle (Hanwoo) and 246 Holstein cattle from 452 farms located all over the country, Korea, were tested for N. caninum antibodies using a commercial enzyme-linked immunosorbent assay (ELISA) kit. Seven of 610 (1.1%) Hanwoo and 54 of 246 (22.0%) Holstein cattle were seropositive.

Determination of Sulfur Dioxide in Herb Drugs

  • Ko, Suk-Kyung;Seung, Hyun-Jeung;Yu, In-Sil;Kim, Hwa-Soon;Park, Noh-Woon;Kang, Hee-Gon
    • Proceedings of the PSK Conference
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    • 2003.10b
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    • pp.103.1-103.1
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    • 2003
  • This study was carried out to investigate sulfur dioxide in herb drugs.(Puerariae Radix, Lycii Fructus, Platycodi Radix, Dioscoreae Rhizoma, Mori Radicis Cortex, Nelumbo Seed, Paeoniae Radix, Remotiflori Radix, Astragali Radix, Polygonatum Rhizome) Experimental subjects included 105 cases of 10 species of herb drugs collected in Kyung-dong market from December in 2001 to February in 2002. Sulfur dioxide was determined by Optimized Monier-Wiliams method. In 65 cases(61.9%) of the 105 cases, we detected $SO_2$ over 10mg/kg. (omitted)

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Monitoring and Risk Assessment of Benzo(a)pyrene Content in Medicinal Herbs (유통 한약재 중 벤조피렌 함량 모니터링 및 안전성 평가)

  • Lee, Sae Ram;Kim, Ae Kyung;Kim, Sung Dan;Lee, Hyun Kyung;Lee, Hee Jin;Ryu, Hoe Jin;Lee, Jung Mi;Yu, In Sil;Jung, Kweon
    • Korean Journal of Pharmacognosy
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    • v.48 no.3
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    • pp.237-242
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    • 2017
  • In this study, to investigate of safety for Benzo(a)pyrene in medicinal herb, 8 kinds of selected commercial herbal medicines (Rehmanniae Radix, Rehmanniae Radix Preparata, Amomi Tsao-Ko Fructus, Cimicifugae Rhizoma, Cyperi Rhizoma, Magnoliae Cortex, Scutellariae Radix, Scrophulariae Radix) were analysed using the high performance liquid chromatography with fluorescence detector and assessed the health risk. The levels of benzo(a)pyrene were from non-detection to $28.1{\mu}g/kg$, and the average was $3.6{\mu}g/kg$. Based on a nationwide survey of the consumption of medicinal herb by the Korean population, we estimated the potential risk from the ingestion of benzo(a)pyrene. The estimated daily intake of benzo(a)pyrene was 1.6 ng/kg b.w./day for group only know the daily average intake of medicinal herb. The MOE (margin of exposure) of benzo(a)pyrene for estimate of health risk was $1.93{\times}10^5$. Therefore, health risk from benzo(a)pyrene through intake of herbal medicine was considered negligible.

Mornitoring of 1-hexene and 1-octene in Hygienic Polyethylene-based Packaging (폴리에틸렌계 위생용 용기포장재중의 1-hexen과 1-octene 조사)

  • Cho, Sung-Ja;Kim, Ae-Kyung;Kwak, Jae-Eun;Kim, Ji-Young;Kim, Si-Jung;Kum, Jin-Young;Kim, Il-Young;Kim, Jung-Hun;Chae, Young-Zoo
    • Journal of Food Hygiene and Safety
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    • v.26 no.4
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    • pp.383-387
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    • 2011
  • The levels of migration of 1-hexene and 1-octene residues in PE (polyethylene) products were analyzed by Headspace gaschromatography (HSGC). A total of 21 samples were including lap, polyglove, zipper bag and ect. The samples were eluted with distilled water, 4% acetic acid, 20% ethanol and n-heptan. The limit of detection (LOD) was 0.06 mg/L ~ 0.30 mg/L and limit of quantitation (LOQ) was 0.21 mg/L ~ 1.01 mg/L, respectively. But because of the high volatile, n-heptan elution was not detected 1-hexene and 1-octene standard. 1-hexene and 1-octene were not detected in the sample which eluted with simulant at $60^{\circ}C$, 30min. Microwave for 1 minute also treated sample and direct heated at $100^{\circ}C$ without simulant were not detected.

Isolation frequency and antimicrobial resistance of Escherichia coli & Enterococcus spp. isolated from beef & pork on sale in Seoul, Korea (서울지역에서 유통 중인 쇠고기와 돼지고기로부터 분리된 대장균과 장구균의 분리율 및 항생제 내성)

  • Kim, Ju-Young;Park, Mi-Ae;Kim, Jee-Eun;Chae, Hee-Sun;Park, Yeon-Jae;Son, Jang-Won;Yang, Yun-Mo;Choi, Tae-Seok;Lee, Ju-Hyung
    • Korean Journal of Veterinary Service
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    • v.36 no.2
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    • pp.111-119
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    • 2013
  • The aim of this study was to investigate the isolation frequency and antimicrobial resistance of Escherichia coli (E. coli) and Enterococcus spp. from domestic beef and pork on sale in Seoul, Korea. A total of 106 (10.4%) E. coli and 114 (11.2%) Enterococcus spp. from 635 domestic beef and 381 pork samples were isolated and examined for susceptibility to 15 and 11 antimicrobial agents, respectively. The most frequent antimicrobial resistance observed in E. coli isolates was to ampicillin (38.6%), followed by streptocmycin (34.9%) and tetracycline (32.0%). The most frequent antimicrobial resistance in E. faecium isolates was to erythromycin (53.8%) and rifampin (46.1%), and in E. faecalis isolates was to tetracycline (55.7%) and rifampin (55.0%). Among the isolates, multi-drug resistant (MDR) E. coli and Enterococcus spp. strains showing resistances to more than two antimicrobial agents tested were 10.4% and 11.2%, respectively. As a result, appropriate protocols for antimicrobial agents and strategies to reduce antimicrobial resistance will be needed in future.

The Simultaneous Analysis of Sennoside A and Rhaponticin in Rhei Rhizoma using Liquid Chromatography-Diode Array Detecter (HPLC/DAD를 이용한 대황의 Sennoside A와 Rhaponticin 동시분석)

  • Kim, Ouk-Hee;Kwak, Jae-Eun;Jung, Sam-Ju;Kim, Dong-Gyu;Han, Eun-Jung;Han, Chang-Ho;Kim, Bok-Soon;Choi, Byung-Hyun;Kim, Min-Young
    • YAKHAK HOEJI
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    • v.54 no.1
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    • pp.39-48
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    • 2010
  • The objective of this study was to analyze sennoside A and rhaponticin simultaneously according to sennoside A of Rhei Rhizoma determination in Korean Pharmacopoeia. $NaHCO_3$ solution in KP was compared with methanol which usually used as solution to extract rhaponticin in Rhei Rhizoma. The method was validated through the guidelines of linearity, LOD, LOQ, specificity and accuracy. Two solution weren't different about validation parameter and passed. So this method were applied to the determination of 6 commercial Rhei Rhizoma samples and 2 samples were suitable for the legal standards.

Phenolic compounds and antioxidant activity of berries produced in South Korea

  • Lee, Yongcheol;Lee, Jea-Kyoo;Kim, Jeong-Gon;Park, So-Hyun;Kim, Young-Eun;Park, Sung-Kyu;Kim, Moo-Sang
    • Journal of Applied Biological Chemistry
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    • v.63 no.4
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    • pp.297-303
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    • 2020
  • Berries are rich sources of phenolic compounds, which are known to have health-promoting effects. In this study, phenolic compounds of seven popularly consumed berries were analyzed by HPLC-ESI-MS/MS. In addition, 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical and nitrite scavenging activities were investigated for comparison of the biological activity of the berry extracts. Chlorogenic acid was the major phenolic acid, which had the highest content in aronia, followed by blueberry. The rutin and isoquercetin contents were the highest in mulberry, followed by aronia, black raspberry, and blueberry. The anthocyanin content was the highest in black raspberry. Aronia showed the highest DPPH free radical and nitrite scavenging activities with the highest contents of total phenolics and proanthocyanidins.

Evaluation of Residual Pesticides in Fresh Ginseng Collected in Seoul

  • Kim, Tae-Rang;Park, Ki-Hwan;Jang, Mi-Ra;Choi, Young-Hee;Kim, Eun-Hee;Choi, Chae-Man;Park, Sung-Kyu;Yu, In-Sil;Hwang, In-Sook;Han, Ki-Young;Kim, Moo-Sang;Kim, Jung-Hun;Chae, Young-Zoo
    • Journal of Applied Biological Chemistry
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    • v.56 no.1
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    • pp.29-35
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    • 2013
  • This study was performed to analyze 48 kinds of pesticide residues using gas chromatography (GC)/nitrogen phosphorous detector, GC/micro electron capture detector, GC/mass selective detector, and high performance liquid chromatograph/diode array detector in 186 fresh ginseng samples collected in the Seoul area from 2010 to 2011. Fresh ginseng dietary intakes were estimated using the data from the 2009 Korea National Health and Nutrition examination survey. Residual pesticides were detected in 79 samples (42.5%) with eight different fungicides. Only 20 samples (10.8%) exceeded the maximum residue limits (MRLs) for pesticides registered by the Korea Food & Drug Administration. Among them, tolclofos-methyl residues (10.2%) exceeded the MRL for fresh ginseng in 18 ginseng seedlings and one of the two-year old fresh ginseng plants, and the residual level in just one ginseng seedling violated the MRL for pyrimethanil. The results showed that residual pesticides levels in marketable fresh ginseng around Seoul were relatively safe. The percent acceptable daily intake (%ADI) was calculated using pesticide residues in fresh ginseng and dietary intakes of fresh ginseng. The risk caused by pesticide residues in fresh ginseng was very low.