• Clinical and Experimental Reproductive Medicine
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• 제27권2호
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• pp.117-131
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• 2000

Objectives: To develop a new immunohistochemical marker system for supplementation of the Noyes histological classification of the endometrium in women of child bearing age with regular menstrual cycles, and to employ this system to evaluate pathologic factors involved in endometriosis, and thus to ascertain if it is useful in diagnosis. Materials and Methods: Endometrial biopsies were sampled from the posterior fundus of 41 (24 proliferative phases, 17 secretory phases) women with regular menstrual cycles (28-32 days), and each sample was immunohistochemically stained according to Noyes et al (1975) for determination of expression for estrogen receptor (ER), progesterone receptor (PR), integrin ${\alpha}_1$, ${\alpha}_4$, ${\beta}_3$, COX-1 and COX-2. Then, the PR, integrin ${\beta}_3$ and COX-2 which were clearly expressed in the luteal phase was with endometrial samples were obtained from 20 cases of normal patients (group 1) and 25 cases with endometriosis (group 2) after confirming the day of ovulation by sex steroid level measurements 7-8 days after ovulation Results: In the regular menstruation group the expression of ER showed a tendency to be increased in the proliferative phase and decreased in the secretory phase, and was the highest in the proliferative phase. However, PR in the stromal cells showed no change in the entire menstrual cycle while in the epithelial cells, PR reached a peak in the late proliferative phase and was almost absent in the secretory phase. Integrin (${\alpha}_1$, ${\alpha}_4$, and ${\beta}_3$ expression in the epithelial cells was absent in the proliferative phase but ${\alpha}_1$ was strongly expressed starting from the early secretory phase into the entire secretory phase. ${\alpha}_4$ was expressed strongly in the early and mid secretory phases and disappeared in the late proliferative phase, while ${\beta}_3$ appeared after the mid secretory phase and continued to be expressed until the late secretory phase. Expression in the stromal cells was weak overall and did not show any cyclic pattern. COX-1 expression was shown as a cyclic pattern in the stromal and epithelial cells and was particularly strongly expressed in the mid secretory phase of epithelial cells, and in the mid secretory and menstruation phase of stromal cells. In the endometrial epithelial cells there was strong expression during the entire cycle with stronger expression in the secretory phase compared to the prolferative phase. COX-2 was clearly expressed in the late proliferative, early and mid secretory phases in the stromal cells. No expression was observed in the proliferative phase of the epithelial cells, but which began to appear in the early secretory phase reaching a significant pattern from the mid secretory phase onwards. There was almost no expression in the stromal cells. In the cases with endometriosis showing normal endometrial maturation according to the Noyes classification, PR expression was increased while Integrin-${\beta}_3$의 expression was significantly decreased compared to the normal group. Also, COX-2 expression was slightly decreased in the stromal cells of patients with endometriosis while it was significantly increased in the stromal cells. Conclusion: Immunohistochemical markers can supplement the original Noyes classification of histological endometrial dating and therefore ascertain existing pathologic conditions. Particularly for patients with endometriosis with normally mature endometrial cells, changes in COX-2 and integrin expression patterns may assist in elucidating pathophysiologic mechanisms and therefore aid in the diagnosis of abnormal implantation conditions, and consequently determine a treatment modality.

• 지역사회간호학회지
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• 제16권3호
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• pp.289-299
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• 2005

Purpose: This study was carried out to identify premenstrual discomforts and coping patterns and their effects. Method: The participants of this study were 297 female university students in C area. The Menstrual Discomfort Questionaire (MDQ) and coping method lists were used as measurement tools. Results: There were significant differences premenstrual discomforts according to age (F=5.76, p=.003) and according to health condition (F=3.43, p=.034). The mean scores of the sub-categorical factors of premenstrual discomfort were 2.35 points for pain, 2.29 for instability and 2.25 for water retention. The worst symptoms among the subcategories of premenstrual discomfort were as follows: backache (M=2.68) in the pain subcategory, irritability (M=2.53) in the instability subcategory and swelling(M=2.40) in the water retention subcategory. Their common coping patterns were 'coping according to menstrual period', 'active behavioral coping' and 'evasional coping'. Frequently used coping methods were 'taking a rest and sleep (99.3%)' and 'taking a warm shower (86.2%)'. Effective coping methods were 'taking a rest and sleep (89.5%)' and 'taking a warm shower (87.1%)'. Conclusion: Most participants have their own coping pattern. Some methods were very effective to PMS but some were not effective to PMS. To manage PMS, effective coping methods should be encouraged for female university students. To keep up with effective coping, education and counseling should be continued. It is considered necessary to make the same research with a larger number of samples and more specified assessment.

• Korean Journal of Acupuncture
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• 제33권3호
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• pp.102-113
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• 2016

Objectives : This study is aimed at developing and discussing the prediction model of blood stasis pattern of traditional Korean medicine(TKM) using machine learning algorithms: multiple logistic regression and decision tree model. Methods : First, we reviewed the blood stasis(BS) questionnaires of Korean, Chinese, and Japanese version to make a integrated BS questionnaire of patient-reported outcomes. Through a human subject research, patients-reported BS symptoms data were acquired. Next, experts decisions of 5 Korean medicine doctor were also acquired, and supervised learning models were developed using multiple logistic regression and decision tree. Results : Integrated BS questionnaire with 24 items was developed. Multiple logistic regression models with accuracy of 0.92(male) and 0.95(female) validated by 10-folds cross-validation were constructed. By decision tree modeling methods, male model with 8 decision node and female model with 6 decision node were made. In the both models, symptoms of 'recent physical trauma', 'chest pain', 'numbness', and 'menstrual disorder(female only)' were considered as important factors. Conclusions : Because machine learning, especially supervised learning, can reveal and suggest important or essential factors among the very various symptoms making up a pattern identification, it can be a very useful tool in researching diagnostics of TKM. With a proper patient-reported outcomes or well-structured database, it can also be applied to a pre-screening solutions of healthcare system in Mibyoung stage.

• 대한한의학원전학회지
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• 제28권3호
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• pp.107-124
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• 2015

Objectives : The purpose of this study is to identify Yangsaeng level and pattern identification of subfertility in childbearing aged women according to the pregnancy experience. Methods : Data was collected by structured questionnaire distributed to 217 women(125 women experienced pregnancy, 92 subfertile women) in I city from Nov. to Dec. 2014. For data analysis, Chi-square test, Fisher's exact test, t-test and Pearson's correlation coefficients were performed using SPSS version 19.0. Results : Drinking rate and stress level of subfertile group were higher than pregnancy experience group. Subfertile women group have more disease related to reproductive system, irregularities of the menstrual cycle and dysmenorrhea than pregnancy experience group. Subfertile women showed lower scores for Yangsaeng level but higher scores for all types of pattern identification of subfertility more than those in the women experiencing pregnancy. Conclusions : The results of this study indicate that reproductive health program for the childbearing aged women is necessary before serviced by western or oriental medical treatment for subinfertile(infertile) women. These results can be used for a program based on oriental medicine in order to improve women's health.

• 방송공학회논문지
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• 제21권3호
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• pp.404-411
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• 2016

본 논문에서는 우리나라 FM(Frequency Modulation) 방송국에서 송신용 안테나로 사용하고 있는 CP(circularly polarized)-dipole 안테나와 CP(circularly polarized)-ring 안테나에 대해 실제 전계 강도 측정을 통한 안테나의 전파 방사 패턴을 조사하고, 현장 적용에 대해 비교 고찰하였다. 비교 결과, 송·중계소가 방송국의 방송 서비스 지역의 한 가운데에 위치하는 경우에는 CP-dipole 안테나(4방향)가 유용하며, 후방에 있는 철탑의 반사 효과로 인해 지향 특성을 갖는 CP-ring 안테나는 지향 방향의 조정을 통하여 타 지역으로의 전파 월경 방지는 물론 주요 서비스 방향으로 5∼9dB 높은 전파를 방사할 수 있었다. 특히, 송·중계소가 서비스 구역의 한 쪽 방향에 위치하여 3방향으로 전파를 방사하는 경우가 많은 우리나라에서는 CP-ring 안테나를 사용하여 안테나의 지향 방향을 인구 및 도로 밀집 지역으로 집중하여 방송 수신 환경을 개선시킬 수 있다.

• Clinical and Experimental Reproductive Medicine
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• 제29권3호
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• pp.209-214
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• 2002

Objective : To analyze the clinical characteristics of obese infertile women. Material and Method: Height, weight, body mass index, menstrual pattern, glucose, insulin, glucose/insulin ratio, dehydroepiandrosterone sulfate (DHEA-S), testosterone, free testosterone and plasminogen activator inhibitor (PAI-1) of 15 obese infertile women were tested. Results: Of 15 obese infertile women, the number of diabetes mellitus, hyperinsulinemia, and insulin resistance was 2 (13%), 2 (13%), 2 (13%), respectively. The incidence of increased DHEA-S, testosterone, and free testosterone was 7 (47%), 1 (7%), 6 (40%), respectively. Notably, all patients showed increased PAI-1. Conclusions: Obesity is associated with infertility as well as many kinds of health problems. Obesity is closely related to insulin resistance and it also causes hyperandrogenism. Increased PAI-1 is one of the important causes of thrombophilia. Consequently, in the workup of obese infertile patient, many aspects of health problems should be considered.

• 대한한방부인과학회지
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• 제32권1호
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• pp.48-72
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• 2019

Objectives: This study was conducted to comprehend the syndrome differentiations of dysmenorrhea and find out their clinical symptoms, tongue images and pulse patterns by analyzing previous studies. Methods: The following researches were collected by searching the medical journals published from November, 2007 to October, 2017, from KISS, OASIS, CNKI. : researches on the syndrome differentiation of dysmenorrhea, researches on the criteria of diagnosis of syndrome differentiation of dysmenorrhea, randomized controlled trials (RCT) used syndrome differentiation for treating dysmenorrhea. Results: By investigating the frequency of syndrome differentiations used in RCT studies, the frequent ones were chosen. They were qi stagnation and blood stasis (氣滯血瘀), qi-blood deficiency (氣血虛弱), congealing cold with blood stasis (寒凝血瘀), liver-kidney depletion (肝腎虧損), blood stasis with dampness-heat (濕熱瘀阻). Conclusion: 4 syndrome differentiations were frequently used in RCT studies. And the frequency of clinical symptoms on each syndrome differentiations from each RCT study was analyzed and compared. Clinical symptoms chosen as chief symptoms in more than one reference, appeared in more than half of the references, most frequent tongue images and pulse conditions were organized. The most frequent clinical symptoms included the period and pattern of pain, the accompanying symptoms of whole-body and the pattern of menstrual bleeding.

• 한국수정란이식학회:학술대회논문집
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• 한국수정란이식학회 2002년도 국제심포지엄
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• pp.112-112
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• 2002

Under normal conditions, women produce a single dominant follicle that participates in a single ovuation each menstrual cycle. But Polycystic ovary syndrome(PCOS) conditions, folliculogenesis does not proceed normally. This condition leads to the accumlation of large numbers of small graffian follicles in which the theca interstitial cells (TIC) produce abnormally large amounts of androgen. PCOS is probably the most common endocrine disorder, affecting women of reprodutive age with 5-10% prevalence estimate. Chronic anovulation, hyperandrogenism, hirsutism, obesity, infertility and polycystic ovaries are clinical hallmarks of women with PCOS. Its etiology remains unknown. To investigate the gene expression pattern of ovary in PCO-induced rat, we used cDNA expression analysis. Total RNA was extracted from the ovary of PCO-induced rat and reverse-transcribed in the presence of［$\alpha$$^{32}$P］-dATP Which were hybridized to Atlas$^{TM}$ Rat Toxicology 1.2 array (Clontech) representing approximately 1176 rat genes. We compared gene expression between ovary of pco-induced immature female rats and control. Differential gene expression profiles were revealed (LIFR-alpha, ADRA1A, Heat shock 90-kDa protein A, PDGFRA). Reverse transcription-polymerase chain reaction(RT-PCR) was used to validate the relative expression pattern obtained by the cDNA array. The precise relationship between the altered expression of genes and PCO is a matter of further investigation. This study was supported by Korea Science and Engineering Foundation(KOSEF)

• Clinical and Experimental Reproductive Medicine
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• 제12권2호
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• pp.25-37
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• 1985

Four points on the basal body temperature (B.B.T.) curve was correlated with the estimated time of ovulation, as determined by serial ultrasound in 50 induced menstrual cycles from 22 subjects. The time of ovulation was estimated by measuring the maximal diameter of follicles and observing the morphologic changes within the ovary from follicle to corpus luteum. The results were as following; 1. The diameter of the follicle measured at the day before disappearance was 21.1 mm on an average (S.D.: 2.14). The average follicular growth for 4 days before ovulation was measured at a rate of 2.8 mm/day, and rapid growth of follicle was observed 3.1 mm/day at the day before. 2. The changes associated with rupture of the follicles were the followings, in order of frequency; decrease in size(94%), disappearance of follicles(64%), fluid in the Cul-de-Sac(26%) and increased internal echoes(16%). 3. Only 20 of 50 cycles, exhibited a BBT dip and correlated with the estimated time of ovulation by ultrasound in 2 of which cases(10%). BBT nadir, 30 of 50 cycles, correlated in 5(16.7%). The first day of hyperthermic plateau(FDHP) and BBT coverline was exhibited in all cycles, correlated in 41(82%) and 35(70%) cases. 4. The relationship between the diameter of dominant dominant follicle, measured by ultrasound, and the basal body temperature curve were as following. During cycles in which dip was observed on the BBT curve, the follicular diameter were 10.5${\pm}$2.12 mm on 4 days prior to the point (D-4), and 12.5${\pm}$2.12 mm (D-3), 15.5${\pm$2.12 mm (D-2), 17.0${\pm}$1.41 mm (D-1) and 21.5${\pm}$2.12 mm just prior to the dip (D-0). In the nadir; 9.6${\pm}$1.67 mm (N-4), 12.8${\pm}$1.79 mm (N-3), 16.2${\pm}$1.92 mm (N-2), 18.2${\pm}$2.17 mm (N-1) and 21.4${\pm}$2.61 mm (N-0). In the First day of Hyperthemic Plateau (FDHP); 9.8${\pm}$1.36 mm (F-4), 12.4${\pm}$1.41 mm (F-3),15.1${\pm}$1.57 mm (F-2), 18.1${\pm}$1.67 mm (F-1) and 21.2${\pm}$2.25 mm (F-0). In the BBT coverline endopint; 9.9${\pm}$.39 mm (C-4), 12.5 ${\pm}$1.44 mm (C-3), 15.2${\pm}$1.64 mm (C-2), 18.0 ${\pm}$1.69 mm (C-1), and 21.2${\pm}$2.31 mm (C-0). 5. The relationship between the ultrasonographic signs of ovulation and the basal body temperature curve were as following. The BBT dip correlated with the ovulation in 2 cases, which revealed decrease in follicular diameter (100%), fluid pattem in the Cul-de-Sac (1 case, 50%) and complete disappearance of follicle (1 case, 50%). In the nadir (5 cases); the ultrasonographic signs of ovulation were decrease in follicular diameter (5 cases, 100%), fluid pattern in the Cul-de-Sac (1 case, 20%) and complete disappearance of follicle (3 cases, 60%). In the First day of Hyperthermic Plateau (41 cases); decrease in follicular diameter (40 cases, 97.6%), fluid pattern in the Cul-de-Sac (11 cases, 26.8%), appearance of internal echo and thickening of the wall (6 cases, 14.6%) and com plete disappearance of follicle (28 cases, 68.3%). In the BBT coverline endpoint (35 cases); decrease in follicular diameter (33 cases, 94.3%), fluid pattern in the Cul-de Sac (9 cases, 25.7%), appearance of internal echo and thickening of the wall (5 cases 14.3%) and complete disappearance of follicle (20 cases, 57.1%).

• 문화기술의 융합
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• 제4권2호
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• pp.185-189
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• 2018

오늘날 현대인들은 20~30대의 운동부족, 40대의 갱년기, 50대의 오십견 같은 운동 부족으로 인한 질병 발생률이 매년 증가하고 있는데, 그 이유는 스마트폰의 발전과 같은 새로운 기술들의 출시로 인해 인간에게 새로운 생활 습관이 생기고, 그에 따른 삶의 패턴이 편리하게 변하여 활동량이 줄어 듬으로, 운동량의 부족이 큰 영향을 끼친다. 이런 인간의 패턴을 기존의 스마트폰과 혼합현실을 융합하여 새로운 혼합현실 헬스케어 서비스 제품을 연구하고자 하는데, 이는 단지 기존에 나온 헬스케어 제품처럼 사람 몸에 부착하여 운동량의 수치를 보여주는 것 이 아닌 운동을 재미있고 간단하게 할 수 있도록 유도해줌과 동시에 운동량을 측정 및 분석하여 사람들의 건강관리에 도움이 되고자 본 연구를 개발하고자 한다.