• 한국소음진동공학회:학술대회논문집
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• 한국소음진동공학회 2004년도 추계학술대회논문집
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• pp.1036-1039
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• 2004

This paper is to experimentally study on the sound absorption of a membrane resonance type system. Membrane resonance type system improves the weak point of a perforated plate system. The experimental results for a membrane resonance type system are explained in comparison with those of a perforated plate system. From the experimental results, it is found that there is an influence of the membrane on the absorption performance. The sound absorbing performances of a membrane resonance system are similar to those of a perforated plate system.

• 대한기계학회논문집
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• 제18권12호
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• pp.3202-3218
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• 1994

Mode shapes and natural frequencies of vibrating laminated composite plates are taken using real-time and time-average holographic interferometry. Debonds and delamination in the laminated plates are measured nondestructively. During holographic testing of composite plates, it has been found that the conditions for the local resonance in debonds are strongly dependent on the frequency of excitation. A membrane resonance model was proposed to describe this behavior. Relations between characteristic length according to the size, shape of debonds and membrane resonance frequency are presented. Several experiments were performed to verify the membrane resonance model. The agreements between the predicted excitation frequency and the observed resonance frequency are good. The experimental results show that higher stresses and strains due to local resonance lead to the debond detection.

• BMB Reports
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• 제38권5호
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• pp.591-594
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• 2005

One of the small proteins from Helicobacter pylori, HP1242, was investigated by the solution nuclear magnetic resonance (NMR) spectroscopy. HP1242 is known as a 76-residue conserved hypothetical protein and its function cannot be identified based on sequence homology. Here, the results of the backbone $^1H$, $^{15}N$, and $^{13}C$ resonance assignments of the HP1242 are reported using double- and triple-resonance techniques. About 95% of all of the $^1HN$, $^{15}N$, $^{13}CO$, $^{13}C{\alpha}$, and $^{13}C{\beta}$ resonances that cover 75 non- Proline residues of the 76 residues are clarified through sequential- and specific- assignments. In addition, three helical regions were clearly identified on the basis of the resonance assignments.

• 한국자기공명학회논문지
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• 제16권2호
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• pp.103-110
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• 2012

Membrane proteins play a essential role in the biological systems and it is not easy to handle a membrane protein for its structural study. Solid-state NMR (ssNMR) can be a good tool to investigate the structures and dynamics of membrane proteins. In ssNMR, Magic Angle Spinning (MAS) and Cross Polarization (CP) can be utilized to reduce the line-broadening, leading to high resolution and sensitivity in the spectrum. ssNMR, if combined with other spectroscopic methods, can provide us a enough knowledge on structures and dynamics of membrane proteins in biological condition.

• 한국해양공학회지
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• 제15권2호
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• pp.22-32
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• 2001

The numerical investigation of obliquely incident wave interactions with fully submerged dual buoy/porous-membrane floating breakwaters placed in parallel with spacing is studied based on linear potential theory and Darcy's law. The numerical solutions are obtained by using a discrete-membrane dynamic model and second-kind modified Bessel function distribution over the entire boundaries of fluid regions. First, numerical solutions for an idealized dual submerged system without buoys are obtained. Second, a more practical dual submerged system with membrane tension provided by buoys at its tops is investigated by the multi-domain boundary element method particularly devised for dual buoy/porous-membrane problems with gaps. The velocity potentials of wave motion are coupled with porous-membrane deformation, and solved simultaneously since the boundary condition on porous-membrane is not known in advance. The effects of varying permeability on membranes and wave characteristics are discussed for the optimum design parameters of systems previously studied. The inclusion of permeability on membrane eliminates the resonances that aggravate the breakwater performance. The system is highly efficient when waves generated by the buoys and membranes were mutually canceled and its energy at resonance frequency dissipates through fine pores on membranes.

• 한국자기공명학회논문지
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• 제13권1호
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• pp.1-6
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• 2009

Syndecan-4 cytoplasmic domain was tested to confirm the interactions with the bilayer membrane using $^{31}P$ solid-state NMR measurements. Syndecan-4 was known as a coreceptor with integrins in the cell adhesion. The syndecan-4 V region is not understood of its functional roles and tested its ability of the interaction with multilamellar vesicles. The $^{31}P$ powder pattern was dramatically changed and showed isotropic peak which imply the bilayer membrane changed its topology to the micelle-like structure. Especially, phosphatidylcholine membrane was affected this effect more than phosphatidylethanolamine membrane.

• 한국자기공명학회논문지
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• 제12권2호
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• pp.96-102
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• 2008

Direct quantification of methanol in polymer electrolyte membrane (PEM) by solid-state nuclear magnetic resonance (NMR) spectroscopy was studied and the methanol concentrations in PEM produced by crossover and diffusion were compared. The error range of the quantification was not smaller than ${\pm}15%$ and the amount of the methanol crossed over in our direct methanol fuel cells (DMFCs) was less than the methanol diffused to PEM. The methanol concentration in the PEM of the DMFC operated at different current densities were equivalent.

• 한국자기공명학회논문지
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• 제19권3호
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• pp.137-142
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• 2015

NMR-based structural studies on membrane proteins are appreciated quite challenging due to various reasons, generally including the narrow dispersion of NMR spectra, the severe peak broadening, and the lack of long range NOEs. In spite of the poor biophysical properties, structural studies on membrane proteins have got to go on, considering their functional importance in biological systems. In this review, we provide a simple overview of the techniques generally used in structural studies of membrane proteins by solution NMR, with experimental examples of a helical membrane protein, caveolin 3. Detergent screening is usually employed as the first step and the selection of appropriate detergent is the most important for successful approach to membrane proteins. Various tools can then be applied as specialized NMR techniques in solution that include sample deteuration, amino-acid selective isotope labeling, residual dipolar coupling, and paramagnetic relaxation enhancement.

• 한국자기공명학회논문지
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• 제28권3호
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• pp.10-14
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• 2024

Caveolin3, mainly expressed in muscle tissue types, is a structural scaffolding protein of caveolae which are microdomains of plasma membrane. To elucidate the relationship between structure and function, several studies on the structure of caveolins using NMR have been reported. Because the ionic strength can affect the electrostatic-driven association of proteins with ligand and protein structure, the effect of salt in the structural studies has to be considered. In this work, we observed that the chemical shifts of Cav3 in the LPPG detergent change depending on salt concentration. The R2 values also show salt concentration-dependent changes. Specifically, in the N-terminal region where conformational changes and various interactions occur, the R2 values decrease. Interestingly, the R2 values of residues expected to be located in the LPPG detergent are also influenced by the salt concentration. This work suggests that the concentration of NaCl can affect interpretation of NMR data from membrane proteins.

• 마이크로전자및패키징학회지
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• 제9권3호
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• pp.37-41
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• 2002

본 연구에서는 자기 공명 특성을 이용한 박막 센서의 멤브레인 및 박막 센서의 특성을 알아보았다. 멤브레인으로 유망한 물질로 $Si_xN_y$과 SiC가 있으며, 최적의 멤브레인 형성 조건을 알고자 박막의 잔류 응력 및 물성을 비교 분석하였다. 그 중에서 박막 센서에 적용 가능한 멤브레인으로 SiC보다 적절한 인장응력과 낮은 열팽창 계수를 가지는 $Si_xN_y$이 센서의 구조층으로 우수하였으며, $Si_xN_y$위에 박막 센서 물질을 증착 및 패턴닝(patterning)을 함으로써 센서의 자기 이력 곡선 및 자기 공진 주파수를 분석하였다. 센서에 인가되는 외부 자기장을 제거하면 자장에 의해 형성된 자화(magnetization)가 탄성 모드로 바뀌면서 에너지 방출에 의해 센서에서 전압이 발생하는데 이때 전압 발생 구간이 길어지면 기계적 진동이 야기되어 신호가 발생한다. 그리고 센서의 길이 및 폭이 증가할수록 자기공명 주파수가 감소하는 것을 볼 수 있다.