• Korean Journal of Animal Reproduction
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• v.20 no.2
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• pp.207-214
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• 1996

To examine the developmental capacity of manipulated embryos after ultrarapid refreezing and thawing, mouse embryos were biopsied at 4-cell stage, frozen twice at 4-cell and morula stages, respectively, and then transferred to rec-ipients. Single blastomeres were biopsied from 4-cell embryos by a modified aspiration method. Biopsied 4-cell embryos were equilibrated into freezing medium at room temperature for 2.5 min, loaded into 40 $\mu$I of freezing medium in 0.25 ml plastic straw and then directly immersed into liqiud nitrogen. Freezing medium for 4-cell embryos consisted of 4.0 M ethylene glycol and O.25 M sucrose in dPBS supplemented with 6 mg/lm BSA. Morulae were frozen into freezing medium containing 5.0 M glycerol instead of ethylene glycol. Thawing was conducted by agitating each straw in 3TC water for 20 sec. The c content of each straw was expelled into 0.5 ml of dilution medium, which consisted of 0.25 M sucrose and 3 mg/ml BSA in dPBS. The thawed embryos were rehydrated in dilution medium for 10 min, washed 3 times with dPBS and then cultured in M16 medium at 37$^{\circ}C$, 5% CO$_2$ in air. Blastocysts that developed from frozen or refrozne biopsied embryos were transferred to recipients on Day 3 of pseudopregnancy, respectively. In vitro and in vivo developmental rates of the biopsied and intact 4~cell embryos after freezing and thawing were 78 (10l/130) and 25% (10/40), and 91 (114/125) and 30% (12/40), respectively. Although the rates of in vitro development of biopsied and intact embryos to blastocyst stage were significantly different after freezing and thawing (P

• Korean journal of food and cookery science
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• v.11 no.1
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• pp.69-76
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• 1995

This study was concentrated on the subject of chou formation and physicochemical characteristics on medium flour mixed with 0, 25, 50, 75, 100% of rice flour in order to clarify the possibility to substitute rice flour for wheat flour on chou preparation. The water holding capacity, swelling power, and maximum viscosity were higher in rice flour than those in medium flour but the initial pasting temperature was equal to 65$^{\circ}C$ in the two flour groups. The ratio of setback during cooling became 0.94 in the rice flour and 1.14 in the medium flour. So, the rice flour showed a slow tendency during gel formation as compared with the medium flour. The volumes of the rice choux were ranged from 80.0% to 89.0% according to the mixing ratio of medium flour as compared with that of the chou of wheat flour. But, the choux formation were increased as much as 108.8% out the paste added gelation of glutinous substance and 124.4% at the paste added Span20 of emulsifier compared to the non-addition treatment. The paste of rice flour added gelatin and Span20 showed better dispersion of components, especially, the small granules of lipid were fairly or plentifully dispersed in the paste added Span20 due to emulsifying activity. In sensory evaluation, the chou of l00% rice flour was inferior to that of medium flour on cavity-forming but the choux of wheat flour mixed with 25%, 50%, and 75% of rice flour were equal or superior to that of medium flour on all characteristics tested such as appearance, surface color, cavity-forming, chewiness, and taste. There were no significant differences on the cavity-forming expansion and taste between choux of rice flour and wheat flour Therefore, the results of this study made conclusion that rice flour would be substituted for wheat flour on the chou preparation.

• Korean Journal of Childcare and Education
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• v.10 no.3
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• pp.31-46
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• 2014

The purpose of this study is to analyze publicness by the types of childcare centers, and factors related to publicness in the operation of childcare centers. The subjects of this study were 511 childcare centers. Data were analyzed by frequency, percentage, mean, standard deviation, F-test, Duncan's post hoc test, and regression. The findings of the study were: First, Among childcare centers, Public childcare centers were high in universality, transparency and participation. But family daycare homes and private centers were low in universality, transparency, participation, and openness. Second, public childcare centers, family daycare homes, private childcare centers, major city and medium-sized cities affected universality in the operation of childcare centers. Public childcare centers, social welfare foundation childcare centers, family daycare homes, private childcare centers and proportion of capacity affected transparency in the operation of childcare centers. Public childcare centers, family daycare homes, private childcare centers and proportion of capacity affected participation in the operation of childcare centers. Government able to accomplish publicness in the operation of childcare centers if it would strengthen management process and expanded financial for childcare centers.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.7 no.1
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• pp.1-21
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• 2013

The release of IEEE 802.15.4e specification significantly develops IEEE 802.15.4. The most inspiring improvement is the enhancement for medium access control (MAC) sublayer. To study the performance of IEEE 802.15.4e MAC, in this paper we first present an overview of IEEE 802.15.4e and introduce three MAC mechanisms in IEEE 802.15.4e. And the major concern here is the Time Slotted Channel Hopping (TSCH) mode that provides deterministic access and increases network capacity. Then a detailed analytical Markov chain model for TSCH carrier sense multiple access with collision avoidance (CSMA-CA) is presented. Expressions which cover most of the crucial issues in performance analysis such as the packet loss rate, energy consumption, normalized throughput, and average access delay are presented. Finally the performance evaluation for the TSCH mode is given and we make a comprehensive comparison with unslotted CSMA-CA in non-beacon enabled mode of IEEE 802.15.4. It can validate IEEE 802.15.4e network can provide low energy consumption, deterministic access and increase network capacity.

• Microbiology and Biotechnology Letters
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• v.43 no.3
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• pp.291-295
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• 2015

Berry or vegetable juices contain a diverse range of antioxidants. Through oxygen radical absorbance capacity assays, acai berry, aronia, wild grape, blackberry, cranberry, and spinach juices were verified to possess high antioxidant activities. Lactic acid bacteria fermentation was applied to each juice as the sole medium to improve antioxidant activity. After fermentation by Lactobacillus plantarum, the antioxidant activities of acai berry, blackberry, and spinach juices increased by 20–30% from 943.2 to 1239.2, from 110.87 to 128.04, and from 77.92 to 107.20 µmol TE/g, respectively. In this study, we found that the antioxidant activities of a number of juices were enhanced through lactic acid bacteria fermentation.

• Polymer(Korea)
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• v.25 no.5
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• pp.642-648
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• 2001

An attempt was made to synthesize an ammonia adsorbent by the photo-induced grafting of styrene (St) onto polypropylene (PP) nonwoven using benzoin ethyl ether (BEE) as a photosensitizer with urea and trimethylol propane triacrylate in methanol medium. As styrene concentration was increased, the graft yield was increased. It was also found that the graft yield increased with reaction time. The polypropylene grafted with styrene (PP-g-St) was sulfonated by chlorosulfonic acid in dichloroethane and complexed with several metal ion, such as $cO^{+2}$, $nI^{+2}$, $cU^{+2}$, $Zn^{+2}$. The amount of ammonia gas adsorbed by these sample was dependent on the degree of sulfonation, adsorption time, and ammonia gas pressure. The adsorption capacity of ammonia gas by the sulfonated PP-g-St(SPP-g-St) nonwoven with 4. 25 mmol $H^+$/g was 6.61 mmol/g. Metal ion complexed SPP-g-St nonwovens had higher adsorption capacity than SPP-g-St nonwoven and the $Co^{+2}$ complexed SPP-g-St showed 9.90 mmol $NH_3$/g, which was much higher than that of active carbon or silica gel.

• Journal of the Korean Society of Marine Environment & Safety
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• v.14 no.4
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• pp.325-331
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• 2008

Recently, new shipbuilding yards with medium capacity are established due to influence of prosperous condition no shipbuilding industry in Korea. These shipbuilding yards have characteristics such as high capacity facilities and followed system of dock launching just like major shipbuilding yards. The present paper explains the dry dock design and structural strength analysis by using FEM. The design stage used TRIBON M3 system while the estimation of structural strength used ANSYS Multiphysics module. The dry dock gate did not frequently open and close, it had to sustain against external wave load during building a ship. Moreover, documents related to design of dry dock gate have no guidelines about design criterion and process of structural strength calculation. It is necessary to make the guidelines about design of dry dock gate. It is expected that this paper will be a good reference to the design of dry dock gate.

• Journal of Satellite, Information and Communications
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• v.11 no.3
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• pp.104-109
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• 2016

A technology of GEO satellite communications starts from Koreasat program in Korea. Payload equipment of EQM Ku and Ka band transponders had been developed and space-qualified Ka band payload in COMS was successfully launched in June, 2010. For the purpose of military communications, Dehop-Rehop transponder was developed in Koreasat5 as ANASIS system and DAT(Digital Active Transponder) and DCAMP(Digital Channel AMPlifier) transponders are now under development. In this paper, from the study of military satellite communications trend, a direction of military communication satellite is suggested based on the current GEO SATCOM technologies in Korea. Considering the limit of frequency resources, a technology of battlefield adaptive transponder with medium capacity against high moveable jamming tactics would be efficient for the future military SATCOM system. Mid-sized military satellites with frequency hopping and mid-capacity transponders can be a solution of vitalizing the GEO satellite programs.

• Journal of Communications and Networks
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• v.2 no.1
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• pp.76-84
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• 2000

Communication systems, including cell-based mobile communication systems, multiple satellite communication systems of multi-beam satellite systems, require reliable handoff methods between cell-to-cell, satellite-to-satellite of beam-to-team, respectively. Recent measurement of a CDMA cellular system indicates that the system is in handoff at about 35% to 70% of an average call period. Therefore, system reliability during handoff is one of the major system performance parameters and eventually becomes a factor in the overall system capacity. This paper presents novel and improved techniques for handoff in cellular communications, multi-beam and multi-satellite systems that require handoff during a session. this new handoff system combines the soft handoff mechanism currently implemented in the IS-95 CDMA with code and packet diversity combining techniques and an iterative decoding algorithm (Turbo Coding). the Turbo code introduced by Berrou et all. has been demonstrated its remarkable performance achieving the near Shannon channel capacity [1]. Recently. Turbo codes have been adapted as the coding scheme for the data transmission of the third generation international cellular communication standards : UTRA and CDMA 2000. Our proposed encoder and decoder schemes modified from the original Turbo code is suitable for the code and packet diversity combining techniques. this proposed system provides not only an unprecedented coding gain from the Turbo code and it iterative decoding, but also gain induced by the code and packet diversity combining technique which is similar to the hybrid Type II ARQ. We demonstrate performance improvements in AWGN channel and Rayleigh fading channel with perfect channel state information (CSI) through simulations for at low signal to noise ratio and analysis using exact upper bounding techniques for medium to high signal to noise ratio.

• Asian-Australasian Journal of Animal Sciences
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• v.16 no.12
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• pp.1822-1829
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• 2003

Porcine colostrum and milk were separated into the acid-soluble and casein fractions by acidification followed by centrifuge. The acid-soluble fraction of porcine colostrum was further separated by liquid chromatography and anisotropic membrane filtration. Trypsin and chymotrypsin inhibitory capacity in porcine colostrum, milk and their components was determined by incubating bovine trypsin or chymotrypsin in a medium containing their corresponding substrates with or without addition of various amounts of porcine colostrum, porcine milk or their components. The inhibition of insulin-like growth factor I (IGF-I) and epidermal growth factor (EGF) degradation in pig small intestinal contents by porcine colostrum was measured by incubating iodinated IGF-I or EGF with the intestinal contents with or without addition of porcine colostrum. Degradation of labeled IGF-I or EGF was determined by monitoring the generation of radioactivity soluble in 30% trichloroacetic acid (TCA). The results showed that porcine colostrum had high levels of trypsin and chymotrypsin inhibitory activity and increased the stability of IGF-I and EGF in pig intestinal contents. The inhibitory activity declined rapidly during lactation. It was also found that trypsin and chymotrypsin inhibitory activity and the inhibition on IGF-I and EGF degradation in the acid-soluble fraction were higher than that in the casein fraction. Heat-resistance study indicated that trypsin inhibitors in porcine colostrum survived heat treatments of $100^{\circ}C$ water bath for up to 10 min, but exposure to boiling water bath for 30 min significantly decreased the inhibitory activity. Compared with the trypsin inhibitors, the chymotrypsin inhibitors were more heatsensitive. Separation of the acid-soluble fraction of porcine colostrum by liquid chromatography and anisotropic membrane filtration revealed that the trypsin and chymotrypsin inhibitory capacity was mainly due to a group of small proteins with molecular weight of 10,000-50,000. In conclusion, the present study confirmed the existence of high levels of protease inhibitors in porcine colostrum, and the inhibition of porcine colostrum on degradation of milk-borne growth factors in the pig small intestinal tract was demonstrated for the first time.