• 한국작물학회지
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• 제48권4호
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• pp.303-307
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• 2003

Some Compositae plants are known to contain biologically active substances that are allelopathic to weeds species as well as antioxidant to foods. Aqueous extracts from leaves of 3 plant species, Cirsium japonica, Kalimeris yomena, and Lactuca saliva, were bioassayed against alfalfa (Medicago saliva) to determine their allelopathic effects. The extracts applied on filter paper in bioassay significantly inhibited root growth of alfalfa. Extracts of 20 g dry tissue $\textrm{L}^{-1}$ from Lactuca sativa showed the most inhibitory effect on alfalfa seedling growth and followed by Cirsium japonica and Kalimeris yomena. Oxidative stability by Rancimat method and antioxidant activity by TBA method for the ground samples were the greatest in Lactuca sativa although were less than that of a commonly used antioxidant, 1% ascorbic acid. Antioxidant activity of methanol extracts on storing meat was stably kept for 28days and was excellent compared to control. These results suggest that three Compositae plants have potent allelopathic and antioxidant effects, and that their activities differ, depending on plant species.

• 한국초지조사료학회지
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• 제13권4호
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• pp.274-277
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• 1993

본 시험은 석회(0, 250, 500, 1,000kg/10a)와 인산(0, 17, 34kg/10a) 시용이 alfalfa(Medicago sativa L.)의 생장 및 질소고정에 미치는 영향을 구명하기 위해 전남대학교 농과대학내 온실에서 pot로 수행되었다. 파종후 9과에 alfalfa의 지상부와 지하부의 건물중 및 아세틸렌 환원력에 있어서 석회 및 인산시용효과가 나타났으며(p<0.05). 파종후 14과(개화초기)에는 alfalfa의 지상부와 지하부의 건물중에 있어서 석회시용 효과는 인정되지 않았으나 인산시용이 증가함에 따라서 건물중은 증가하였다(p<0.01). 또한 석회 및 인산시용이 증가함에 따라서 아세틸렌 환원력은 유의적으로 증가하였다(p<0.05). 파종후 9과에서 전 질소함량은 석회 및 인산 시용구에서 무시용구보다 감소되었으며(p<0.05), 파종후 14과에서는 석회 시용구는 무시용구보다 전 질소함량이 증가되었으나 인산 시용구는 무시용구 보다 낮아지는 경향을 보였다(p<0.05).

• 한국초지조사료학회지
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• 제20권4호
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• pp.275-284
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• 2000

To investigate the effect of Mycorrhiza (Glomus intradics) and Rhizobium inoculation on the N, P utilization and the growth of Alfalfa (Medicago sativa L.), four treatments (non-inoculation, control ; Mycorrhiza inoculation, M ; Rhizobiurn inoculation, R and Mycorrhiza and Rhizobium inoculation, M+R) were applied. The associated analyses were carried out on the early vegetative growth stage (DAS 56, 56 days after seeding) and on the early flowering stage (DAS 126). The decreased rate of total N and P content in soil, with advancing plant growth, was relatively higher in the mycorrhiza andor rhizobium treatments than control. The content of available phosphorus in soil at DAS 126 increased by about 40 % in M and M+R treatment compared to control (141.0 mg PzOg'kg DM), while non-significant changes was observed in R treatment. Total N uptake and P uptake in the control at DAS 126 were 33.91 mglplant and 2.42 mdplant, respectively, about 21, 50 and 51 % of increases in total N uptake and 30, 11 and 47 % of increases in total P uptake were estimated in M, R and M+R treatment. Comparing to control, dry matter yield significantly increased by 8, 27 and 28 %, and crude protein yield also by 21, 42 and 39 %, respectively, in M, R and M+R treatment. The present data indicated that mycorrhiza orland rhizobium inoculation improved N, P utilization, and consequently increased the yield of alfalfa. (Key words : Alfalfa, Mycorrhiza, Rhizobium, N and P Utilization, Growth, Yield)

• 한국작물학회지
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• 제34권1호
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• pp.48-54
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• 1989

외국에서 도입한 32개의 서로 다른 Alfalfa 품종을 우리나라 기후풍토에서 재배하여 초장과 건물중 등의 생육상태를 비교 검사한 결과 가장 우수한 생육상태를 나타낸 품종들은 다음과 같다. 1. 초장생육이 우수하였던 상위 7개 품종들은 Diamond>Milkmaker> Arrow>Chalienger>Sapphire > Dekalb brand 135> LS-1920의 품종순이고 최하위 초장의 7개 품종을 하위순으로 열거하여 보면 Pioneer brand 5929> Maxidor> Dekalb brand 167> Pierce> Blazer> Raidor> Pioneer brand 532의 순이었다. 2. 건물중이 많은 상위 7개 품종은 Pike>LS-1920>Challenger>Salute>Diamond>Decathlon>Pioneer brand 581의 품종순이며 최하위 건물중의 7개 품종은 Maxidor>Pioneer brand 5929>Pioneer brand 532>Florida 77> Pierce> Magnum> Blazer의 순이었다. 3. 초장과 건물중의 결과를 비교하여 보면 대체로 초장에서 우수하였거나 열거하였던 품종들이 역시 건물중에서도 우세하거나 열거하여 서로가 비례하는 결과를 보였다. 4. 이상에서 조사한 바와 같이 가장 좋은 생육상태 및 수활량을 보인 품종들은 Pike, LS-1920, Challenger, Salute, Diamond 등이었으며 이들중 Challenger 품중을 제외한 나머지 품종들은 내한성이 중간정도인 Semi-dormant 계통으로 우리 나라 재배환경에 가장 적합할 것으로 생각된다.

• 한국초지조사료학회지
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• 제21권1호
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• pp.27-30
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• 2001

알팔파 (Medicago sativa L.)의 줄기부분을 절단하여, SH 배지에 IBA를 첨가한 농도별로 뿌리 유도 정도를 조사한 결과, IBA 농도를 1.0~1.5 mg/$\ell$로 첨가한 처리구에서 뿌리 유도 정도가 70~75%로서 가장 좋았다. 캘러스로부터 shoot를 유도한 다음 줄기를 취하여 처리한 경우에는. 처리 10일째에 1/2 SH-0 배지에서 10개 (50%), SH-0 배지에서 10 (50%), SH-0.5IBA 배지에서 12개 (60%), SH-1.0IBA 배지에서 15개 (75%), SH-1.5IBA 배지에서 15개 (75%), SH-2.0IBA 배지에서 10개 (50%), SH-2.5IBA 배지에서 9개 (45%), SH-3.0IBA 배지에서 8개 (40%)의 뿌리 유도를 나타내었다.

• Journal of Animal Science and Technology
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• 제63권4호
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• pp.799-814
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• 2021

Information is lacking about the effect of date of a fall cut of alfalfa (Medicago sativa L.) on dry matter yield (DMY), forage nutritive value, and stands persistence. The objective of this study was to determine the effect of timing of a fall cut on DMY, forage nutritive value and stand persistence of three alfalfa varieties: low-lignin Hi-Gest 360, Roundup Ready Tonica, and conventional Gunner in Northeastern Kansas in the United States. The field study was carried out by splitting plot in randomized complete block design with four replications. The harvesting data of different maturity stages were collected in each year from 2015 to 2018. Three cuts were harvested based on the stage of maturity, and the last (fourth) cut was done on September 15, September 30, October 15, and October 30 of each year. The persistence of the alfalfa stands was determined each fall after the last cut, and each spring after the first cut, by counting the number of live plants in a randomly placed quadrat in each plot. Alfalfa cut on September 15 and September 30 had a higher stand persistence compared to alfalfa cut on October 15 and October 30. The DMY of the first cut in 2016 was significantly higher in roundup ready than the low-lignin alfalfa variety. In the second cut, DMY was significantly higher in conventional alfalfa than the roundup ready. There were no significant differences in DMY between alfalfa varieties in the rest of seasonal cuttings in 2016 and 2017 and annual total yield in both years. In general, low lignin alfalfa variety had higher crude protein and relative feed value and lower acid detergent fiber and neutral detergent fiber contents than those in roundup ready and conventional alfalfa varieties. On average, nutritive value of alfalfa was generally affected by last cutting dates in 2017. Based on 3-year data the last cutting of alfalfa in the fall could be done by September 30-October 15 without harmful effect on DMY.

• 한국작물학회지
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• 제45권2호
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• pp.134-138
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• 2000

Most parts of alfalfa plant have been reported to contain autotoxic substances that inhibit seed germination and early seedling growth, however, the chemical(s) is not still studied much. Effect of seed leachates of 'Vernal' alfalfa (Medicago sativa L.) was evaluated for inhibition of alfalfa germination and root growth through bioassay. Alfalfa seeds were extracted in 1 L deionized water for 1 h after soaking and the leachates caused to reduce root length of alfalfa significantly as the soaking time increased. Crude seeds at 4 g L$L^{-1}$ exudated autotoxins that reduce significantly root length by 34 % compared to the control, when the seeds soaked in deionized water for 24 h. However, the extracts did not affect final germination as well as speed of germination. Extracts from ground seeds significantly reduced speed of germination (GT 50) and root length. The results indicate that release of autotoxic substances from seeds during seed imbibition was increased with increase of soaking time and seed amount, and that autotoxicity was more occurred in ground seeds than in crude seeds.

• Plant Resources
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• 제8권2호
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• pp.116-121
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• 2005

Compositae plants are known to contain biologically active substances that are allelopathic to agricultural crops as well as weed species. Aqueous or methanol extracts and plant residues from leaves of Xanthium occidentale were assayed against alfalfa (Medicago sativa) to determine its allelopathic effects, and the results showed that the extracts applied onto filter paper significantly inhibited seed germination as well as root growth of alfalfa. Aqueous leaf extracts of $40g\;L^{-1}$ were completely inhibitory on root growth of alfalfa, while root growths of barley (Hordeum vulgare L.) and soybean (Glycine max L.) were less sensitive. Leaf residue incorporation at $100g\;kg^{-1}$ into soil on seedling growth of barnyard grass (Echinochloa crus-galli Beauv. var. oryzicola Ohwi) inhibited both shoot and root fresh weights of barnyard grass by 94 and $96\%$, respectively. Methanol extracts from BuOn and EtOAc fractions of X. occidentale reduced alfalfa root growth more than did those from hexane and water fractions. The results based on bioassay of extracts and residues show that X. occidentale had potent an allelopathic activity against other plant species.

• Asian-Australasian Journal of Animal Sciences
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• 제27권6호
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• pp.825-831
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• 2014

The aim of this study was to determine the nutritive value of some legume species in salt-affected soils of South-East Anatolian region using chemical composition and in vitro gas production kinetics. In this study, Lotus corniculatus, Trifolium alexandrinum, Medicago sativa were sown and tested in four different locations. A 3 by 4 factorial design with 3 legume species and 4 salt levels (non salty electrical conductivity (EC)<4 dS/m; low salt: 4 dS/m>EC<8 dS/m, medium saline: 8 dS/m>EC<16 dS/m and high salt: 16 dS/m>EC) was used in the study. Results indicated that salinity and plants had no significant effect on ash and ether extract. Dry matter (DM), acid detergent fiber, digestible dry matter, dry matter intake (DMI) were affected by plant, salinity and plant${\times}$salinity interaction. On the other hand neutral detergent fiber, relative feed value (RFV), and DMI were affected by salinity and plant${\times}$salinity interaction. Mineral contents were affected by plant species, salinity and salinity${\times}$plants interactions. In vitro gas production, their kinetics and estimated parameters such as were not affected by salinity whereas the gas production up to 48 h, organic matter digestibility, metabolizable energy (ME), and net energy lactation ($NE_L$) were affected by plant and plant${\times}$salt interaction. Generally RFVs of all species ranged from 120 to 210 and were quite satisfactory in salty conditions. Current results show that the feed value of Medicago sativa is higher compared to Lotus corniculatus and Trifolium alexandrinum.

• Journal of Animal Science and Technology
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• 제46권3호
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• pp.437-442
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• 2004

콩과목초는 젖소의 중요한 단백질 공급원이며 토양에는 질소비료원이다. 본 시험은 콩과목초의 사초생산성과 품질을 비교하기 위하여 천안연암대학의 실습농장에서 실시하였다. 공시한 콩과목초 5초종은 2모작이 가능한 목초로 크림손 클로버(Trifolium incarnatum L.) 'Tibbee', 헤어리 베치(Vicia villosa Roth) 'Common', 레드클로버(Trifolium pratense L.) 'Kenland', 페르시안 클로버(Trifolium resupinatum L.) 'Leeton', 알팔파(Medicago sativa L.) 'Vernal' 이다. 콩과목초의 내한성은 크림손 클로버가 7점인 것을 제외하고는 공시품종 모두가 8점 이상으로 양호하였다. 콩과목초의 개화기는 크림손 클로버가 4월 23일로 공시초종 중에서 가장 빨리 개화하였으며, 헤어리 베치는 5월 13일, 레드 클로버는 5월 3일, 알팔파는 5월 12일에 개화하였다. 그러나 페르시안 클로버는 수확시까지 출수를 하지 않았다. 수확시 건물률은 크림손 클로버가 17.6%로 공시초종 중에서 가장 높았다. 건물 및 TDN 수량은 크림손 클로버와 헤어리 베치가 공시초종 중에서 가장 많앗다. 조단백질 함량은 헤어리 베치가 20.5%로 가장 높았다. NDF 및 ADF 함량은 페르시안 클로버가 35.5 및 25.0%로 가장 적었다. NDF와 ADF를 근거로 계산한 상대사료가치(RFV)는 헤어리 베치를 제외하고는 콩과목초 모두가 125이상으로 특급이었다. 페르시안 클로버는 공시초종에서 상대사료가치와 TDN이 가장 높은 양질의 콩과목초였다. 이상의 결과를 종합해 볼때 콩과목초 중 크림손 클로버와 헤어리 베치가 사초수량이 높았고, 페르시안 클로버가 품질이 우수하였다.