• Proceedings of the Korean Society of Crop Science Conference
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• 1996.10a
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• pp.102-103
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• 1996

• The Korean Journal of Ecology
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• v.27 no.1
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• pp.43-47
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• 2004

This research was investigated to prepare basic data in a study on the copper tolerance of herbaceous plants through the growth rate and the elimination rate dependent on Cu concentration of 6 species; Commelina communis, Medicago sativa, Echinochloa frumentancea, Zea mays, Helianthus annuus and Abutilon avicennae, We examined the germination rate, root and shoot growth of seedling and fresh biomass of 6 species (Commelina communis, Medicago sativa, Echinochloa frumentancea, Zea mays, Helianthus annuus and Abutilon avicennae) painted to Cu contaminated soil (50, 100, 200, 300-CuCl₂/㎏) and control for 14 days. The germination rate of H. annuus, E. frumentancea and C. communis were not affected by Cu concentration. However, root and shoot growth of H. annuus was about 7% of control and the biomass was 35% of control at 300 ㎎-CuCl₂/㎏. E. frumentancea and C. communis that showed good growth rate at higher Cu contaminated soil (>200 -CuCl₂/㎏), were the most tolerant plant to Cu concentration. Especially, E. frumentancea eliminated over 30% of Cu in soil and the amount of Cu uptake increased with increasing Cu concentration; 1,020㎎ Cu per 1 ㎏ of soil at 300 ㎎-CuCl₂/㎏. From these results, we concluded that E. frumentancea would be used for phytoremediation.

• Journal of The Korean Society of Grassland and Forage Science
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• v.13 no.4
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• pp.274-277
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• 1993

We investigated the effects of applications of various levels of lime(0, 250, 500 and 1,000 kg/10a) and phosphate (0, 17 and 34 kg/10a) on growth and nitrogen fixation of alfalfa (Medicago sativa L.). Effects of lime and phosphate applications were significantly different on dry matter (DM) weight of each part and on acetylene reduction activity (ARA) of alfalfa at 9 weeks alter sowing (p<.05). The effect of lime on DM of shoot and root was not significantly different at 14 weeks after sowing (early bloom stage), but that of phosphate on DM was significantly improved as increasing of phosphate levels (p<.01). The effects of lime and phosphate on ARA were significantly increased (p<.05). Application of lime and phosphate decreased total nitrogen (TN) content of each part of alfalfa at 9 weeks after sowing (p<.05). The effects of lime application on TN was higher but that of phosphate application on TN was lower than no application of lime or phosphate at 14 weeks after sowing (p<.05).

• Journal of The Korean Society of Grassland and Forage Science
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• v.11 no.3
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• pp.145-152
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• 1991

This experiment was carried out to investigate the effects of liming, manure application, and inoculation with Rhizobium on the early growth, nodulation, seedling establishment, winter survival, and forage yield and quality of alfalfa(Medicago sativa L.) at the Experimental Livestock Farm, College of Agriculture, Seoul National University, Suweon in 1985 and 1986. The results obtained are as follows: 1. Seedling vigor and nodule formation of alfalfa at early stage were the best in the treatments of liminginoculation( L1) and liming-manure-inoculation(LM1). 2. Seedling establishment was not significantly affected by the treatments of liming, manure application, nodulation and the combination of these. 3. The LI and LMI treatments showed the highest winter survival of 94.3 and 83.5%, respectively, but the lowest winter su.rvival of 55.9 and 58.5% was found in no treatment(None) and inoculation( I ), respectively. 4. No differences in crude protein(CP1, acid detergent fiber(ADF) and neutral detergent fiber(NDF) contents were found among the treatments. 5. There were significant differences in the total dry matter yield of alfalfa among the treatments. The total dry matter yields of 10,618, 11,830, 11,855 and 13,456 kg/ha from the treatments of L, LM, LI and LMI, respectively, were significantly higher than those of None, M and I treatments. 6. Based on the results of the experiment, it appears that the dry matter yield of alfalfa could be enhanced by the treatments of liming with Rhizobium inoculation and also liming, manure application and Rhizobium inoculation as a practical method.

• Journal of The Korean Society of Grassland and Forage Science
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• v.20 no.4
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• pp.275-284
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• 2000

To investigate the effect of Mycorrhiza (Glomus intradics) and Rhizobium inoculation on the N, P utilization and the growth of Alfalfa (Medicago sativa L.), four treatments (non-inoculation, control ; Mycorrhiza inoculation, M ; Rhizobiurn inoculation, R and Mycorrhiza and Rhizobium inoculation, M+R) were applied. The associated analyses were carried out on the early vegetative growth stage (DAS 56, 56 days after seeding) and on the early flowering stage (DAS 126). The decreased rate of total N and P content in soil, with advancing plant growth, was relatively higher in the mycorrhiza andor rhizobium treatments than control. The content of available phosphorus in soil at DAS 126 increased by about 40 % in M and M+R treatment compared to control (141.0 mg PzOg'kg DM), while non-significant changes was observed in R treatment. Total N uptake and P uptake in the control at DAS 126 were 33.91 mglplant and 2.42 mdplant, respectively, about 21, 50 and 51 % of increases in total N uptake and 30, 11 and 47 % of increases in total P uptake were estimated in M, R and M+R treatment. Comparing to control, dry matter yield significantly increased by 8, 27 and 28 %, and crude protein yield also by 21, 42 and 39 %, respectively, in M, R and M+R treatment. The present data indicated that mycorrhiza orland rhizobium inoculation improved N, P utilization, and consequently increased the yield of alfalfa. (Key words : Alfalfa, Mycorrhiza, Rhizobium, N and P Utilization, Growth, Yield)

• Asian-Australasian Journal of Animal Sciences
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• v.22 no.4
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• pp.520-527
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• 2009

Alfalfa (Medicago sativa L) is a high-quality forage for ruminants and the main stem is the dominant morphological component contributing to the forage nutritive value in mature alfalfa forage. Shearing force, a fracturing property of plant stem, is an important indictor of forage value. The objectives of this study were to investigate the effects of morphological characteristic on shearing force, the relationship between shearing force and chemical composition, and the relationship between shearing force and in situ digestibility of alfalfa stem. The results showed that linear density (weight per unit length of stem) was more important than chemical composition in affecting shearing force. There was a positive relationship between lignin content and shearing force (r = 0.78). Correlations were not found between shearing force and other chemical components such as neutral detergent fiber (NDF), acid detergent fiber (ADF), cellulose and hemicelluloses. In situ digestibility (of dry matter and NDF) was related to shearing force. A negative correlation was found between shearing force and dry matter (DM) digestibility (r = -0.70), and there was also a negative correlation between shearing force and NDF digestibility (r = -0.87). When shearing force was standardized for stem diameter or stem linear density, the relationship between shearing force and digestibility was consistent regardless of stem diameter and stem linear density. Shearing force was significantly correlated with lignin content and in situ digestibility (of DM and NDF), and was a more direct indicator for estimating forage nutritive value related to animal performance, so it can be used to predict the forage value of alfalfa.

• Asian-Australasian Journal of Animal Sciences
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• v.27 no.6
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• pp.825-831
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• 2014

The aim of this study was to determine the nutritive value of some legume species in salt-affected soils of South-East Anatolian region using chemical composition and in vitro gas production kinetics. In this study, Lotus corniculatus, Trifolium alexandrinum, Medicago sativa were sown and tested in four different locations. A 3 by 4 factorial design with 3 legume species and 4 salt levels (non salty electrical conductivity (EC)<4 dS/m; low salt: 4 dS/m>EC<8 dS/m, medium saline: 8 dS/m>EC<16 dS/m and high salt: 16 dS/m>EC) was used in the study. Results indicated that salinity and plants had no significant effect on ash and ether extract. Dry matter (DM), acid detergent fiber, digestible dry matter, dry matter intake (DMI) were affected by plant, salinity and plant${\times}$salinity interaction. On the other hand neutral detergent fiber, relative feed value (RFV), and DMI were affected by salinity and plant${\times}$salinity interaction. Mineral contents were affected by plant species, salinity and salinity${\times}$plants interactions. In vitro gas production, their kinetics and estimated parameters such as were not affected by salinity whereas the gas production up to 48 h, organic matter digestibility, metabolizable energy (ME), and net energy lactation ($NE_L$) were affected by plant and plant${\times}$salt interaction. Generally RFVs of all species ranged from 120 to 210 and were quite satisfactory in salty conditions. Current results show that the feed value of Medicago sativa is higher compared to Lotus corniculatus and Trifolium alexandrinum.

• Journal of The Korean Society of Grassland and Forage Science
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• v.16 no.2
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• pp.113-120
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• 1996

The experiment was carried out to investigate the effects of phosphate fertilizer level on growth, crude protein content and nitrogen fixation ability of alfalfa(Medicugo sariva L.) at sowing year. The experimental field was conducted using a randomized block design with three replications according to the phosphate application level (200 and 400kfia) in farm of Kongju National University. Research samples were collected at intervals of 7 days from October 7 to December 9 and nitrogen fixation activity of nodule was measured by using the acetylene reduction method (Hardy et al. 1968). The results obtained are summarized as follows : 1. Number of branches, root length and dry matter weight at phosphate fertilizer level of 400kg in sowing year were more increase than those of 200kfia. The number of nodules was not significantly difference between phosphate fertilizer level, because of low temperature to be enough to nodulation of alfalfa. 2. The crude protein content of leaf, stem and root were not significantly affected by the phosphate fertilizer level. but that of leaf was increased at 400kgha on 72 days after sowing. The crude protein content of each part according to growing stages was increased at 44 days and 72 days after sowing. 3. The dry weight of root was not significantly difference between phosphate fertilizer level from 30 days to 51 days after sowing, but that was increased at phosphate fertilizer level of 400kgha from 51 days afier sowing. 4. The amount of ethylene in the samples taken 2 hours and calculated amount of nitrogen fixation were increased at phosphate fertilizer level of 400kfia from 30 days to 65 days after sowing. but there was vice versa from 58 days after sowing.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.34 no.1
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• pp.48-54
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• 1989

This study was conducted to select the best adapted alfalfa cultivars among 32 introduced alfalfa cultivars in the Korean environmental condition. Plant height and dry matter yield per cultivar were determined. On the point of plant height, seven high ranked cultivars among the 32 alfalfa cultivars in high order were Diamond> Milkmaker> Arrow>Chalienger>Sapphire > Dekalb brand 135> LS-1920. and seven low ranked cultivars in low order were Pioneer brand 5929> Maxidor> Dekalb brand 167> Pierce> Blazer> Raidor> Pioneer brand 532. Seven high ranked cultivars from 32 alfalfa cultivars on the dry matter yield in high order were Pike>LS-1920>Challenger>Salute>Diamond>Decathlon>Pioneer brand 581, and seven low ranked cultivars in low order were Maxidor>Pioneer brand 5929>Pioneer brand 532>Florida 77> Pierce> Magnum> Blazer. Dry matter yield per cultivar was also greater when alfalfa cultivar was appeared higher plant height compared with those which were lower plant height. Leading cultivars for plant growth and yield under the Korean environmental condition were Pike, LS-1920, Challenger, Salute, Diamond among the 32 alfalfa cultivars. All those cultivars except Challenger cultivar are Semi-dormant which can be well grown under moderate temperatures.

• Journal of The Korean Society of Grassland and Forage Science
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• v.21 no.1
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• pp.27-30
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• 2001

When root initiation ratio of cut alfalfa (Medicago sativa L.) stems was examined in several medium conditions containing different IBA concentration, Highest root initiation ratio was confirmed at 1.0~1.5 mg/$\ell$ of IBA and the ratio was 70~75%. When the stems from regenerated shoots from callus were treated at 8 kinds of medium for 10 days, the root iniation result was 10 (50%) at 1/2 SH-0 medium, 10 (50%) at SH-0 medium, 12 (60%) at SH-0.5IBA medium, 15 (75%) at SH-1.0IBA medium, 15 (75%) at SH-1.5IBA medium. 10 (50%) at SH-2.0IBA medium, 9 (45%) at SH-2.5IBA medium and 8 (40%) at SH-3.0IBA medium.