• BMB Reports
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• 제37권6호
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• pp.726-734
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• 2004

White spot disease (WSD) is caused by the white spot syndrome virus (WSSV), which results in devastating losses to the shrimp farming industry around the world. However, the mechanism of virus entry and spread into the shrimp cells is unknown. A binding assay in vitro demonstrated VP28-EGFP (envelope protein VP28 fused with enhanced green fluorescence protein) binding to shrimp cells. This provides direct evidence that VP28-EGFP can bind to shrimp cells at pH 6.0 within 0.5 h. However, the protein was observed to enter the cytoplasm 3 h post-adsorption. Meanwhile, the plaque inhibition test showed that the polyclonal antibody against VP28 (a major envelope protein of WSSV) could neutralize the WSSV and block an infection with the virus. The result of competition ELISA further confirmed that the envelope protein VP28 could compete with WSSV to bind to shrimp cells. Overall, VP28 of the WSSV can bind to shrimp cells as an attachment protein, and can help the virus enter the cytoplasm.

• Journal of Microbiology and Biotechnology
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• 제32권9호
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• pp.1073-1085
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• 2022

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has continued for over 2 years, following the outbreak of coronavirus-19 (COVID-19) in 2019. It has resulted in enormous casualties and severe economic crises. The rapid development of vaccines and therapeutics against SARS-CoV-2 has helped slow the spread. In the meantime, various mutations in the SARS-CoV-2 have emerged to evade current vaccines and therapeutics. A better understanding of SARS-CoV-2 pathogenesis is a prerequisite for developing efficient, advanced vaccines and therapeutics. Since the outbreak of COVID-19, a tremendous amount of research has been conducted to unveil SARS-CoV-2 pathogenesis, from clinical observations to biochemical analysis at the molecular level upon viral infection. In this review, we discuss the molecular mechanisms of SARS-CoV-2 propagation and pathogenesis, with an update on recent advances.

• Journal of Environmental Science International
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• 제21권7호
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• pp.845-853
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• 2012

Diethyl phthalate (DEP) is widely spread in the natural environment as an endocrine disruption chemicals (EDs). Therefore, in this study, ultrasound (US) and ultraviolet (UVC), including various applied power density (10-40 W/L), UV wavelengths (365 nm, 254 nm and 185 nm) and frequencies (283 kHz, 935 kHz) were applied to a DEP contaminated solution. The pseudo-first order degradation rate constants were in the order of $10^{-1}$ to $10^{-4}\;min^{-1}$ depending on the processes. Photolytic and sonophotolytic DEP degradation rate also were high at shortest UV wavelength (VUV) due to the higher energy of photons, higher molar absorption coefficient of DEP and increased hydroxyl radical generation from homolysis of water. Sonolytic DEP degradation rate increased with increase of applied input power and the dominant reaction mechanism of DEP in sonolysis was estimated as hydroxyl radical reaction by the addition of t-BuOH, which is a common hydroxyl radical scavenger. Moreover, synergistic effect of were also observed for sonophotolytic degradation with various UV irradiation.

• Korean Journal of Environmental Biology
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• 제21권3호
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• pp.303-307
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• 2003

Tributyltin (TBT) used world-wide in antifouling paints toy ships is a wide-spread environmental pollutant. At low doses, antiproliferative modes of action have been shown to be involved, whereas at higher doses apoptosis seems to be the mechanism of toxicity in reproductive organs by TBT. In this study, we investigated that the mechanisms underlying apoptosis induced by TBT in R2C cell. Effects of TBT on intracellular $Ca^{2+}$ level and reactive oxygen species (ROS) were investigated in R2C cells by fluorescence detector. TBT significantly induced intracellular $Ca^{2+}$ level in a time-dependent manner. The rise in intracellular $Ca^{2+}$ level was followed by a time-dependent generation of reactive oxygen species (ROS) at the cytosol level. Simultaneously, TBT induced the release of cytochrome c from the mitochondrial membrane into the cytosol. Furthermore, ROS production and the release of cytochrome c were reduced by BAPTA, an intracellular $Ca^{2+}$ chelator, indicating the important role of $Ca^{2+}$ in R2C during these early intracellular events. In addition, Z-DEVD FMB, a caspase -3 inhibitor, decreased apoptosis by TBT. Taken together, the present results indicated that the apoptotic pathway by TBT might start with an increase in intracellular $Ca^{2+}$ level, continues with release of ROS and cytochrome c from mitochondria, activation of caspases, and finally results in DNA fragmentation.

• Journal of Microbiology and Biotechnology
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• 제33권1호
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• pp.15-27
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• 2023

The incidence of melioidosis cases caused by the gram-negative pathogen Burkholderia pseudomallei (BP) is seeing an increasing trend that has spread beyond its previously known endemic regions. Biofilms produced by BP have been associated with antimicrobial therapy limitation and relapse melioidosis, thus making it urgently necessary to understand the mechanisms of biofilm formation and their role in BP biology. Microbial cells aggregate and enclose within a self-produced matrix of extracellular polymeric substances (EPSs) to form biofilm. The transition mechanism of bacterial cells from planktonic state to initiate biofilm formation, which involves the formation of surface attachment microcolonies and the maturation of the biofilm matrix, is a dynamic and complex process. Despite the emerging findings on the biofilm formation process, systemic knowledge on the molecular mechanisms of biofilm formation in BP remains fractured. This review provides insights into the signaling systems, matrix composition, and the biosynthesis regulation of EPSs (exopolysaccharide, eDNA and proteins) that facilitate the formation of biofilms in order to present an overview of our current knowledge and the questions that remain regarding BP biofilms.

• The Journal of Asian Finance, Economics and Business
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• 제8권8호
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• pp.541-552
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• 2021

The purpose of this research is to investigate the mediating role of performance information use in the relationship between result-oriented culture and organizational performance. The data were collected from chief accountants and managers working in public-sector organizations in Vietnam. The analysis was based on the 197 completed questionnaires by using Partial Least Squares Structural Equation Modeling (PLS-SEM). For the test research model and hypotheses, the supporting tool is SmartPLS 3 software. Research findings show that result-oriented culture is positively associated with organizational performance, and that performance information use is a mediating mechanism that explains how a result-oriented culture leads to higher performance in public organizations. The findings of this study suggest that the results-oriented culture, not only directly improves organizational performance, but also indirectly does so by the use of performance information. This study provides evidence for theoretical understanding of the impact of result-oriented culture on the performance of public-sector organizations through performance information use, and suggests practical implications for public organizations in applying result-oriented culture to foster using performance measurement and improve organizational performance. In order to achieve good performance, public-sector organizations need to create and spread result-oriented culture and enhance the use of performance information for decision-making.

• Proceedings of the Korean Institute of Building Construction Conference
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• 한국건축시공학회 2021년도 봄 학술논문 발표대회
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• pp.102-103
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• 2021

In Korea, starting with the Busan residential-commercial fire in 2010, the frequency of fire expansion in high-rise buildings has been continuously increasing. In the case of such large-scale fires, most of the fires generated from the inside tend to expand to the upper floors by riding the exterior material or exterior wall panels through the process of being ejected to the outside. It has been revised so that combustible exterior materials cannot be used in buildings. However, due to the legal fluoride level, the fire risk of high-rise buildings is still high, such as the case of a 33-story residential-commercial fire in Ulsan. In order to prevent such fire expansion, it is considered that it is necessary to first understand the nature of the fire occurring inside and the mechanism of the fire expansion in the upper floor. The purpose of this study is to propose improvements in domestic fire safety design through a review of existing literature to prevent fire expansion of high-rise buildings.

• Health Policy and Management
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• 제30권3호
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• pp.270-276
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• 2020

Entering the fourth industrial revolution era, health technology is rapidly developing and the people's needs for medical services are gradually increasing. Establishing a life cycle management of health technology has emerged as a new policy agenda to cope with these changes. However, the management of health technology have been conducted without continuity and with several problems pointed out. Therefore, we suggest the reform agendas by stages to establish system for a life cycle management of health technology in the fourth industrial revolution era as follows. In the stage of development, it is important not only to provide research funding, but also consulting by professional about whole cycle of health technologies. In the phase of market entry, there are needs for enhance the system that would expand the early adoption for innovative technology and increase its effectiveness. After the spread of health technology to clinical settings, a reassessment and post management system should be established that have an institutional framework with strong price adjustment and exit mechanism. Furthermore, we hope that discussions will be brisk in macro perspective on the balancing of development in healthcare industry, health of people and national health insurance finance.

• The Bulletin of The Korean Astronomical Society
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• 제37권2호
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• pp.91-91
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• 2012

We present high-resolution near-infrared host galaxy spectra of low-z quasars, PG0844+349 (z=0.064), PG1226+023 (z=0.158), and PG1426+015 (z=0.086). The observation was done by using the near-IR high resolution echelle spectrometer, IRCS, at the SUBARU 8.2 m telescope. The full width at half maximum of the point spread function was about 0.3 arcsec by using an Adaptive Optics system, which can effectively resolve the quasar spectra from the host galaxy spectra. The signal-to-noise ratios are increased by the total exposure time up to several hours per targets and the development of data reduction method. We compare our results to the stellar spectra library and sample spectra from Dasyra et al. (2007) and Watson et al. (2008). The identified spectral lines will be used to study the physical mechanism of quasars, and the velocity dispersions of the stars in the bulge of the host galaxy.

• International Journal of Fuzzy Logic and Intelligent Systems
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• 제9권4호
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• pp.249-254
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• 2009

The today's decision making tasks in globalized business and manufacturing become more complex, and ill-defined, and typically multiaspect or multi-discipline due to many influencing factors. The requirement of obtaining fast and reliable decision solutions further complicates the task. Intelligent decision support system (DSS) currently exhibit wide spread applications in business and manufacturing because of its ability to treat ill-structuredness and vagueness associated with complex decision making problems. For multi-dimensional decision problems, generally an optimum single DSS can be developed. However, with an increasing number of influencing dimensions, increasing number of their factors and relationships, complexity of such a system exponentially grows. As a result, software development and maintenance of an optimum DSS becomes cumbersome and is often practically unfeasible for real situations. This paper presents a technically feasible approximation of an optimum DSS through decreasing its complexity by a modular structure. It consists of multiple DSSs, each of which contains the homogenous knowledge's, decision making tools and possibly expertise's pertaining to a certain decision making dimension. Simple, efficient and practical integration mechanism is introduced for integrating the individual DSSs within the proposed overall DSS architecture.