• KOREAN JOURNAL OF CROP SCIENCE
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• v.48 no.6
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• pp.419-423
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• 2003

Bacterial blight caused by Xantomonas oryzae pv. oryzae is one of the most serious diseases of rice especially in southern area of Korea. Three races, $\textrm{K}_1$, $\textrm{K}_2$ and $\textrm{K}_3$, are the most dominant species. lo improve rice breeding efficiency using marker assisted selection, some RFLP markers were surveyed for polymorphism between resistant and susceptible to $\textrm{K}_1$ and $\textrm{K}_3$. And, 127 doubled-haploid (DH) lines derived from Milyang121/HRl1650-1-4-2 and 131 DH lines derived from Milyang123/HR10624-AC5 were evaluated to bacterial blight ($\textrm{K}_1$ and $\textrm{K}_3$). Milyang121 and HR10624-AC5 have Xa-1, resistant to $\textrm{K}_1$ race, and Milyang123 has Xa-3, resistant to $\textrm{K}_1$ and $\textrm{K}_3$ race. Three markers, RZ590, RZ536 and RG303, showing polymorphism between parents and resistance gene, Xa-1 and Xa-3, were analysed in the two combinations of DH lines. The segregation pattern of resistant DH population of Milyang123/HR10624-AC5 to susceptible showed 3:1 and 1:1 in $\textrm{K}_1$ and $\textrm{K}_3$ race. In three RFLP markers, RZ590 was linked to Xa-1 on chromosome 4, and RZ536 and RG303 were linked to Xa-3 on chromosome 11. The map distance between Xa-1 and RZ590 was 3.1cM on chromosome 4, and Xa-3 and RZ536/RG303 were 7.6/16.0cM on chromosome 11, respectively. The results of RFLP mapping will be useful for the selection and pyramiding of bacterial blight resistant genes.

• Horticultural Science & Technology
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• v.35 no.3
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• pp.333-343
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• 2017

Bloomless cucumber fruits are commercially produced by grafting onto the pumpkin stocks (Cucurbita moschata) to restricted silicon ($SiO_2$) absorption. Inhibition of silicon absorption in bloomless stocks is conferred by a mutant allele of the CmLsi1 homologous to Lsi1 in rice. In this study, we characterized the Lsi1 homologs in pumpkin (C. moschata) and its cold-tolerant wild relative C. ficifolia ('Heukjong') in order to develop a DNA marker for selecting a bloomless trait and to establish the molecular basis for breeding bloomless stock cultivars of C. ficifolia. A Cleaved amplified polymorphic sequence (CAPS) marker (CM1-CAPS) was designed based on a non-sysnonymous single nucleotide polymorphism (SNP, C>T) of the CmLsi1 mutant-type allele, and its applicability for Marker-assisted selection (MAS) was confirmed by evaluating three bloom and five bloomless pumpkin stock cultivars. Quantitative RT-PCR of the CmLsi1 for these stock cultivers implied that expression level of the CmLsi1 gene does not appear to be associated with the bloom/bloomless trait and may differ depending on plant species and tissues. A full length cDNA of the Lsi1 homolog [named CfLsi1($B^+$)] of 'Heukjong' (C. ficifolia), was cloned and sequence comparison between CmLsi1($B^+$) and CfLsi1($B^+$) revealed that there exists total 24 SNPs, of which three were non-synonymous. Phylogenetic analysis of CfLsi1($B^+$) and Lsi1 homologs further revealed that CfLsi1($B^+$) is closesly related to Nodulin 26-like intrinsic proteins (NIPs) and most similar to CpNIP1 of C. pepo than C. moschata.

• The Bulletin of The Korean Astronomical Society
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• v.40 no.1
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• pp.75.4-76
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• 2015

우리는 동아시아 각국(대한민국, 중국, 일본)의 VLBI관측망을 통합하고 구성될 동아시아 VLBI 관측망(East Asia VLBI Network; EAVN)의 초기 검증 작업을 진행하고 있다. EAVN은 2 ~ 129 GHz로 관측이 가능하는데, 주로 6.7, 8, 22, 43 GHz로의 관측을 상정하고 있다. 또한 최대 기선장은 약 5,000 km으로 공간분해능은 약 0.3 mas(43 GHz로의 관측의 경우)가 된다. 높은 공간분해능과 고감도의 특징을 활용하고 저광도 활동성은하핵이나 우리 은하의 메이저 천체 등의 연구에 대해서 위력을 나타낼 수가 있다. 우리는 EAVN 시험관측, 상관처리, 자료처리의 실행, 및 그것들을 통한 EAVN 운영의 검증을 하는 EAVN Tiger Team을 2013년에 조직하고 현재까지에 8 GHz 및 22 GHz로 8회의 VLBI 시험관측을 실행하였다. 상관처리는 주로 한국천문연구원에서 운영하고 있는 한일 공동상관기(KJJVC)와 상하이천문대의 소프트웨어 상관기(DiFX)로 실행되어 있다. 현재까지에 8 GHz 및 22 GHz 쌍방에서 프린지검출에 성공하고 있고, 올해는 영상합성을 포함한 과학적인 관측을 진행할 예정이다. 이 발표에서는 EAVN의 개요와 과학목표, 시험관측 현황과 결과, 및 앞으로의 운영 계획 등을 소개하겠다.

• Journal of the Korean Society of Physical Medicine
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• v.6 no.3
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• pp.257-266
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• 2011

Purpose : To assess the relationship between post-stroke shoulder pain, motor function, and pain-related quality of life(QOL) Methods : Volunteer sample of 62 chronic stroke survivors with post-stroke shoulder pain and glenohumeral subluxation. The patients answered the question in shoulder pain with the Brief Pain Inventory question 12 (BP1-12), Pain-related Quality of life(BPI-23). Therapists measured the performance of combined upper-limb movement including the hand-behind-neck(HBN), hand-behind-beck(HBB) maneuver, added passive pain-free shoulder external rotation range of motion, and Modified Ashworth Scale(MAS) score of the elbow flexors. Physical performance assessments were used to measure basic activity daily living(Modified Barthel Index-self care, MBI-S/C), motor function of upper limb(Fugl-Meyer Upper/Lower Extremity, FM-U/E). Results : Stepwise regression analyses indicated that post-stroke shoulder pain is associated with the BPI 23, but not with the FM-U/E, MBI-S/C. Thus, the presence of shoulder pain is more important predicting pain-related QOL than its degree in predicting motor function of upper limb and basic activity daily living. Conclusion : Post-stroke shoulder pain was associated with reduced quality of life related to pain. The pain was not associated with the motor function of upper limb and basic activity daily living. The result imply that management of shoulder pain & anatomical position of shoulder joint after stroke should be emphasized. This provides a further incentive to develop effective rehabilitation prevention and treatment strategies for post-stroke shoulder pain.

• Asian Pacific Journal of Cancer Prevention
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• v.17 no.5
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• pp.2519-2525
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• 2016

There are several existing reports of microarray chip use for assessment of altered gene expression in different diseases. In fact, there have been over 1.5 million assays of this kind performed over the last twenty years, which have influenced clinical and translational research studies. The most commonly used DNA microarray platforms are Affymetrix GeneChip and Quality Control Software along with their GeneChip Probe Arrays. These chips are created using several quality controls to confirm the success of each assay, but their actual impact on gene expression profiles had not been previously analyzed until the appearance of several bioinformatics tools for this purpose. We here performed a data mining analysis, in this case specifically focused on ovarian cancer, as well as healthy ovarian tissue and ovarian cell lines, in order to confirm quality control results and associated variation in gene expression profiles. The microarray data used in our research were downloaded from ArrayExpress and Gene Expression Omnibus (GEO) and analyzed with Expression Console Software using RMA, MAS5 and Plier algorithms. The gene expression profiles were obtained using Partek Genomics Suite v6.6 and data were visualized using principal component analysis, heat map, and Venn diagrams. Microarray quality control analysis showed that roughly 40% of the microarray files were false negative, demonstrating over- and under-estimation of expressed genes. Additionally, we confirmed the results performing second analysis using independent samples. About 70% of the significant expressed genes were correlated in both analyses. These results demonstrate the importance of appropriate microarray processing to obtain a reliable gene expression profile.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.44 no.4
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• pp.345-349
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• 1999

This study was carried out to map Bphl and bph2 gene in Mudgo and Sangju13 (Oryza sativa L.) respectively conferring resistance to brown plan-thopper (BPH) and to establish the marker-assisted selection (MAS) system. Bulked seedling (grown for 20 days) test was conducted with the 73 F4 lines derived from a cross between Nagdongbyeo and Mudgo for Bphl and with 53 BC3F5 lines derived from the Milyang95/Sangju13 cross for bph2. Bph1 was mapped between RG413 and RG901 on chromo-some 12 at a distance of 7.5 cM from RG413 and 8.4 cM from RG90l. A recessive gene bph2 was located near RZ76 on chromosome 12 at a distance of 14.4 cM. Bphl and bph2 were linked to each other with a distance of about 30 cM. An RFLP marker, RG413 linked to Bphl, was converted to an STS marker to facilitate the marker-assisted selection. BPH resistant genotypes could be selected with 92% accuracy in a population derived from a line of NM47-B-B.

• Molecules and Cells
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• v.27 no.1
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• pp.21-37
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• 2009

Map-based cloning to find genes of interest, marker-assisted selection (MAS), and marker-assisted breeding (MAB) all require good genetic maps with high reproducible markers. For map construction as well as chromosome assignment, development of single copy PCR-based markers and map integration process are necessary. In this study, the 132 markers (57 STS from BAC-end sequences, 13 STS from RFLP, and 62 SSR) were newly developed as single copy type PCR-based markers. They were used together with 1830 markers previously developed in our lab to construct an integrated map with the Joinmap 3.0 program. This integrated map contained 169 SSR, 354 RFLP, 23 STS from BAC-end sequences, 6 STS from RFLP, 152 AFLP, 51 WRKY, and 99 rRAMP markers on 12 chromosomes. The integrated map contained four genetic maps of two interspecific (Capsicum annuum 'TF68' and C. chinense 'Habanero') and two intraspecific (C. annuum 'CM334' and C. annuum 'Chilsungcho') populations of peppers. This constructed integrated map consisted of 805 markers (map distance of 1858 cM) in interspecific populations and 745 markers (map distance of 1892 cM) in intraspecific populations. The used pepper STS were first developed from end sequences of BAC clones from Capsicum annuum 'CM334'. This integrated map will provide useful information for construction of future pepper genetic maps and for assignment of linkage groups to pepper chromosomes.

• Proceedings of the Technology Innovation Conference
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• 2005.08a
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• pp.141-167
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• 2005

본 연구의 목적은 우리나라보다 앞서 실시하고 있는 일본의 산업 클러스터 계획 프로젝트의 거시적인 현황과 가장 성공적이라고 하는 TAMA클러스터의 사례를 분석하여 향후 우리나라 클러스터 정책 시행에 필요한 시사점을 얻는데 있다. 특히 TAMA클러스터의 성공은 이를 직접 관리하고 있는 민간차원의 중개조직인 TAMA산업활성화협회의 운영전략을 집중적으로 분석하여 봄으로써 도입 가능성에 대한 진단과 정책적 제안을 하고자 한다. 첫째, 일본의 19개 지역별 산업 클러스터 계획의 공통점은 어느 것도 도도부현의 영역을 초월한 광역적인 클러스터를 형성하고 있다. 광역단위의 집적은 상대적으로 클러스터 계획에 대한 중요성과 실행주체를 망각하기 쉬워 실패할 가능성이 비교적 높을 수 있다 이러한 점을 극복하기 위한 대안으로 산학연의 3주체 간 연계를 주로 담당하는 민간중심의 중개조직을 두어 운영하고 있다. 둘째, 클러스터 정책기간은 시작에서 자립까지 20년을 상정하고, 제1기 5년간은 산업 클러스터 네트워크 구축기, 제2기 5년은 산업 클러스터 성장기 그리고 마지막 제3기 10년은 산업 클러스터의 자율적 발전기로서 책정되어 수행하는 것이 바람직하다고 보고 있다. 셋째, 개별적인 산업 클러스터의 비전, 목표, 중장기 전략의 올바른 수립이 필요한데 백화점식이 아닌 대상산업 및 대상 주체에 대한 선택과 집중의 전략성이 엿보인다. 넷째, 정책 대상의 범위는 연구개발에서 판매에 이르기까지 전 비즈니스 프로세스를 대상으로 하고 있으며 클러스터 관련 정책수행 주체들의 정책조율, 중복 투자의 회피 그리고 효과적인 정책을 시행하기 위하여 수평적인 연락망과 협의체를 구성하고 있다. 다섯째, TAMA협회는 전통적인 동종 산업중심의 협회 기능과는 달리 제품개발형 중소$\cdot$중견기업을 대상으로 신기술개발과 신사업 전개를 위한 전략적 지원을 하고 있다. 비교적 개별적인 기술개발 능력은 물론 독자적인 마케팅 능력을 갖고 있어 일반적인 보호 대상자로서의 중소기업과는 차원이 다르며 기존의 하청관계라는 수직적 의존적인 존재가 아니라 세계적으로 니치 시장에서 경쟁력을 가지고 있는 대기업과 대등한 입장의 우량기업이라는 것이 특징이다. 여섯째, 지금까지는 기업을 지원하는 각 기관들이 각각 독자적으로 운영하여 경영자원의 분산과 이용으로 수혜자인 중견$\cdot$중소기업의 입장은 물론 지방자치단체 및 중앙정부의 차원에서 분석하여 볼 경우 경영자원의 Critical Mass를 확보하기 어려웠다. TAMA협회는 네트워크 조직으로서 주로 소프트적인 지원을 통하여 원활하게 수행하고 있음. 정보와 네트워크만을 가지고 조직간 관계를 중개하는 가벼운 네트워크 조직으로서 운영하는 방법은 한국의 경우에도 적용이 가능하며 매우 유용하다고 생각된다.

• Proceedings of the Korean Society of Dyers and Finishers Conference
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• 2012.03a
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• pp.48-48
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• 2012

화학센서는 분석물질과 감응물질간의 화학적 반응을 통해 분석물질을 선택적으로 인지하고 이를 통하여 특정물질을 실시간으로 분석할 수 있는 기술이다. 최근 화학센서로 색소를 이용하여 음이온을 진단/감응하는 기술이 각광 받고 있으며, 더불어 음이온을 선택적으로 인지함에 있어 검출하고자 하는 특정 음이온에 대한 민감도를 높이기 위한 노력이 계속되고 있다. 감응물질로 이용되는 색소는 주로 분자 내 전하 이동형 색소(intramolecular charge transfer dye)로 주위 환경 변화에 민감하게 반응하며, 자극에 따른 변화를 흡수와 발광, 굴절률의 변화 등으로 나타낸다. 또한 다양한 음이온 중 분석물질로써 연구 가치가 큰 음이온에는 플루오린화물(fluoride)이 있다. 이는 플루오린화물이 치아 보호와 골다공증에 중요한 역할을 하는 순기능을 가지는 반면 고농도 상태에서는 불소증(fluorosis)을 비롯한 악영향을 잠재적으로 가지기 때문에 그 양을 인지하는 것이 중요하게 여겨지기 때문이다. 따라서 본 연구에서는 2-(3,5,5-trimethylcyclohex-2-enylidene)-malononitirle과 indole-3-carboxaldehyde를 통하여 분자 내 전하 이동형 색소를 합성하고, $^1H$ NMR, GC-mas, EA로 합성된 색소의 물성을 분석하였다. 우선 반응물인 2-(3,5,5-trimethylcyclohex-2-enylidene)-malononitirle을 합성하기 위해 dimethylfor mamide(DMF) 용매 하에서 isophorone과 malononitrile을 12시간 반응시키고, 얻어진 결과물을 정제한다. 이후 indole-3-carboxaldehyde와 10시간 환류시켜 색소를 얻는다. 합성된 색소는 F 이온 검출에 이용되며, UV-vis 분광법을 이용하여 분석물질에 따른 흡수 정도와 강도 변화를 살펴본다. 연구의 최종적인 목적은 비단 진단/감응 색소의 합성이 아니라 나노 섬유 소재와 색소의 접합을 통해 진단/감응형 나노 섬유를 개발하는 것으로 이를 위해 전기방사법이 이용된다.

• Proceedings of the Korean Radioactive Waste Society Conference
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• 2004.02a
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• pp.245-256
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• 2004

The extraction of three kinds of amido podands, N,N,N'N'-tetrabutyl-3-oxa-pentanedi- amide (TBDGA), N,N,N'N'-tetra-isobutyl-3-oxa-pentanediamide(TiBDGA) and N,N,N'N'-tetra- butyl-3,6-dioxa-oct-anediam- ide(TBDOODA) on U(VI),Pu(IV), Am(III), Eu(III) and other metal ions is studied in nitric acid solutions. 40%octanol-kerosene is chosen as diluents to eliminate third phase and emulsion. TBDGA and TiBDGA show extraction selectivity to An(III) and Ln(III) much higher than to U(VI) and Pu(IV). Fe, Ru and Mo is poorly extracted by the three kinds of amid podands in 2~3mol/L $HNO_3$ solutions. Aiming to eliminate interface crude when using simulated HLLW solution in the system of 0.2mol/L TBDGA/Octanol＋kerosene, acetohydroxyamic acid was adapted. Distribution ratio of zirconium was decreased when adding acetohydroxyamic acid in aqueous solution, and interface crude disappeared as mixing extractant with HLLW. The counter-current extraction test is carried out in a set of miniature mixer-settler, with 0.2mol/L TBDGA/ 40% octanol-kerosene as extractant to separate U(VI), Pu(IV), Am(III) and Eu(III) from simulated high level liquid waste(HLLW) solution. In battery A, lanthanides and actinides are coextracted into organic phase with the recovery of 99.98% for U(Ⅵ), >99.99% for Pu(IV), and >99.99% for Am(III) and Eu(III) respectively. In battery R1, 99.99% U, 86.2% Pu and a part of Am or Eu are stripped into aqueous phase by 0.2mol/L acetohydroxyamic acid (AHA) in 0.01mol/L $HNO_3$ solution. In battery $R_2$, Am, Eu and remained Pu are completely back-extracted by 0.2mol/L AHA. This separation process contains no salt reagent, and it is not necessary to dilute HLLW feed.