• Journal of Microbiology and Biotechnology
• /
• v.9 no.5
• /
• pp.576-581
• /
• 1999

Effects of signal sequences, protein sizes and dissolved oxygen on the secretion of human lysozyme from a recombinant yeast were experimentally characterized. The systems consisted of Saccharomyces cerevisiae host SEY2102 that was transformed with two different plasmids. These plasmids were identical with an exception to the plasmid pMC614, which contained the native yeast MFα1 sequence and the plasmid pMC632 with the non-native rat α-amylase signal sequence. The expression of human lysozyme was controlled by the ADHI promoter. The native yeast MFαl signal sequence was more efficient than the non-native rat α-amylase signal sequence in directing the secretion of human lysozyme. Lysozyme secreted with the α-amylase signal was retained inside the cells and released to the medium very slowly, thereby causing a lower cell growth rate and a decreased product secretion rate. Lysozyme was secreted more efficiently than invertase, which is an order of magnitude bigger in molecular size compared to lysozyme, which was under the direction of the MFαl signal sequence, suggesting that protein sizes may affect the secretion efficiency. When expressed in anaerobic conditions in the medium where the ADHI promoter was derepressed, the amount of lysozyme secreted was about twice higher than that of the aerobic culture. However, the secretion rates were identical. This result showed that the dissolved oxygen level may affect the efficiency of protein secretion only, and not the secretion rate of the product protein.

• Journal of Korean Society of Environmental Engineers
• /
• v.36 no.11
• /
• pp.733-738
• /
• 2014

Within the scope that does not interfere with the performance of basic duty, the military has been actively supporting the national policy for the conservation of the natural environment in accordance with the "military environmental protection guidelines". Since there is a lack of information regarding inhabitation for wild fauna and flora, field investigations of the natural environment at Jinhae local unit were performed three times in 2003. As a result of the investigation, lycoris sanguinea as flora, marten, otter, and leopard cat as mammalia, and accipiter soloensis, accipiter gentilis schvedow, accipiter gularis, buteo hemilasius, falco tinnunculus and terpsiphone atrocaudata as bird were confirmed to inhabit. For the conservation of the natural ecosystem, the expansion of research for natural ecosystems within the military unit is required. Additionally, environmental pollution can be prevented through the proper maintenance of basic environmental facilities including sewage treatment facilities, soil pollution control facilities, and waste storage facilities. In cooperation with local governments and private organizations, the natural cleansing action and sustainable wildlife conservation should be expanded.

• Journal of Environmental Impact Assessment
• /
• v.21 no.4
• /
• pp.593-607
• /
• 2012

The Ministry of Environment have started the 'National Ecosystem Survey' since 1986. It has been carried out nationwide every ten years as the largest survey project in Korea. The second one and the third one produced the GIS-based inventory of species. Three survey methods were different from each other. There were few studies for species distribution using national survey data in Korea. The purposes of this study are to test species distribution models for finding the most suitable modeling methods for the National Ecosystem Survey data and to investigate the modeling results according to survey methods and taxonominal group. Occurrence data of nine species were extracted from the National Ecosystem Survey by taxonomical group (plant, mammal, and bird). Plants are Korean winter hazel (Corylopsis coreana), Iris odaesanensis (Iris odaesanensis), and Berchemia (Berchemia berchemiaefolia). Mammals are Korean Goral (Nemorhaedus goral), Marten (Martes flavigula koreana), and Leopard cat (Felis bengalensis). Birds are Black Woodpecker (Dryocopus martius), Eagle Owl (Bubo Bubo), and Common Buzzard (Buteo buteo). Environmental variables consisted of climate, topography, soil and vegetation structure. Two modeling methods (GAM, Maxent) were tested across nine species, and predictive species maps of target species were produced. The results of this study were as follows. Firstly, Maxent showed similar 5 cross-validated AUC with GAM. Maxent is more useful model to develop than GAM because National Ecosystem Survey data has presence-only data. Therefore, Maxent is more useful species distribution model for National Ecosystem Survey data. Secondly, the modeling results between the second and third survey methods showed sometimes different because of each different surveying methods. Therefore, we need to combine two data for producing a reasonable result. Lastly, modeling result showed different predicted distribution pattern by taxonominal group. These results should be considered if we want to develop a species distribution model using the National Ecosystem Survey and apply it to a nationwide biodiversity research.

• Animal Systematics, Evolution and Diversity
• /
• v.29 no.2
• /
• pp.99-114
• /
• 2013

Phylogeographic and feeding ecological studies of seven terrestrial mustelid species (Carnivora, Mustelidae), the Japanese marten Martes melampus, the sable Martes zibellina, the Japanese badger Meles anakuma, the ermine or the stoat Mustela erminea, the Japanese weasel Mustela itatsi, the least weasel Mustela nivalis, and the Siberian weasel Mustela sibirica, representing four biogeographic patterns in the Japanese archipelagos (Hokkaido, Honshu-Shikoku-Kyushu, Tsushima, and Hokkaido-Honshu), were reviewed in order to clarify causes for the faunal assemblage processes of those mustelid species in Japan. Here, three main constraints were extracted as important factors on the mustelid assemblage. First, fundamental evolutionary differences maintained by niche conservatism in each ecologically diversified lineage ("evolutionary constraint") would enable the species to co-occur without any major problem (coexistence among Martes, Meles, and Mustela species). Second, "ecological constraints" would force two closely related species to be allopatric by competitive exclusion (Mu. itatsi and Mu. sibirica) or to be sympatric by resource partitions (Mu. erminea and Mu. nivalis). Third and most importantly, "geological constraints" would allow specific species to be embraced by a particular geographic region, primarily deciding which species co-occurs. The allopatric distribution of two Martes species in Japan would have been established by the strong effect of the geological separation in Tsugaru Strait. Elucidating both phylogeny and ecology of co-existing species in a community assemblage is important to know which species possess distinct lineage and which ecological traits are adapted to local environments, fulfilling the requirement of the field of conservation biology that endemism and adaptation should both be considered. The Japanese archipelagos would, therefore, provide valuable insight into the conservation for small carnivoran species.

• Journal of Energy Engineering
• /
• v.28 no.4
• /
• pp.42-47
• /
• 2019

In this study, combustion characteristics were measured by selecting di-n-buthylamine, which is widely used as an emulsifier, insecticide, additive, rubber vulcanization accelerator, corrosion inhibitor, and raw material for dye production. The flash point of the di-n-buthylamine was measured by Setaflash, Pensky-Martens, Tag, and Cleveland testers. And the AIT of the di-n-buthylamine was measured by ASTM 659E. The explosion limits of the di-n-buthylamine was calculated using the measured flash points by Setaflash tester. The flash point of the di-n-buthylamine by using Setaflash and Pensky-Martens closed-cup testers were experimented at 38 ℃ and 43 ℃, respectively. The flash points of the di-n-buthylamine by Tag and Cleveland open cup testers were experimented at 48 ℃. The AIT of the di-n-buthylamine was experimented at 247 ℃. The LEL and UEL calculated by using lower and upper flash points of Setaflash tester were calculated at 0.69 vol% and 7.7 vol%, respectively. The measurement of the flash point measurement and the calculation method of the explosion limit prediction presented in this study can be used to study the fire and explosion characteristics of the other combustible liquids.

• Journal of the Korean Institute of Gas
• /
• v.27 no.4
• /
• pp.50-55
• /
• 2023

Lithium-ion secondary batteries are currently in high demand and supply. The purpose of this study is to secure the safety of the process by studying the combustion characteristics of EC(Ethylene Carbonate), Which is mainly used as an electrolyte organic solvent for lithium ion batteries. The flash points of the EC by using Setaflash and Pensky-Martens closed-cup testers were experimented at 141 ℃ and 143 ℃, respectively. The flash points of the EC by Tag and Cleveland open cup testers were experimented at 152 ℃ and 156 ℃, respectively. The AIT(Auto Ignition Temperature) of the EC was experimented at 420 ℃. The LEL(Lower Explosive Limit) calculated by using lower flash point of Setaflash was calculated at 3.6 Vol.%.

• Journal of the Korean Society of Costume
• /
• v.55 no.4 s.94
• /
• pp.38-48
• /
• 2005

This study is consider·ed about the relation or costume among Parhae, ancient Japan and Tang and the culture of this time commonly was influenced by Tang;s culture. The recorded items, which were exchanged from Parhae to Tang and ancient Japan, are the pelt of marten, seal, bear and tiger skin or Ammowhaha(암모화) Gu(구) and so on. Other items from Tang and ancient Japan to Parhae are various clothes and material like silk and so on. This Parhae's clothes made with leather obtained through hunting were the main product of Parhae and had been greatly famous in Tang and ancient Japan. At that time, Tang and ancient Japan had been only dependent on the agricultural life but had not been accustomed to the hunting life, and thereupon, the people in Tang and Nara Era had difficulties to get leather clothes. Accordingly, the leather products stood for the rich, and the people who purchased the leather products from Parhae were recognized as the rich. On the other hand, The prince and the royal family in Parhae were dispatched to Tang as Sooktwui(숙위). They paid a tribute to Tang and In return for it, Tang gave to Sookwui as well as to Parhae's envoys various items and clothes such as Po(포), belt(대), gold and silver Eodae(어대) and textile like a type of silk. These clothes were considered as the formal uniform like Kwanbok(관복) of Parhae. Specially, the time when Sookwui was sent to Tang rot-responded with the time when Tang gave formal uniform to Parhae's envoys. Therefore, we can confirm that the sending of Sookwui influenced to form uniform system of Parhae. In this exchange relation, there were several duplicated items showed, for example Po(포)-belt(대), Eui set(의일습), Eoeui(어의), Jobok(조복), Go(고). These items in Parhae were appeared in process of exchanging from Tang to Japan and from Tang to Parhae. At that time, the exchanges between Tang and Parhae were frequently active much more than those between Tang and Japan, and the acceptance of the culture from Tang was easier in Parhae than Japan in consideration of the geographical location. Therefore, those clothes could be understood as the items already used in Parhae and affected from Tang. These items in Parhae were appeared in process of exchanging from Tang to Japan and from Japan to Parhae. As a result, it is expected that the costumes, which Parhae took from Japan, are the same as those of Tang.

• Journal of the Korean Society of Environmental Restoration Technology
• /
• v.9 no.3
• /
• pp.51-58
• /
• 2006

The purpose of this research presents a method to position and makes the structure for eco-corridors reasonably with collectable analysing results of various effects shown in mammals' road-kill at 429 points. Target animals of this research are Leopard cat, Siberian weasel, Raccoon dog, Korean hare, Eurasian red squirrel, Siberian chipmunk and Water deer. The results derived from the empirical analysis on the contents above are followed. First, according to the results as for Leopard cat road kill analysis, which is designated as Endangered Species Class II, the eco-corridor might be located at near village having stead food in order to decrease the frequencies of road-kill, because its road kill points were mainly collected at 4 lane hilly road with mountain-road-farm area geological type of. Second, because Siberian weasel's road kill was detected at 2 lane hilly road with mountain-road-stream geological type, the eco-corridor might be located at near a mill to decrease road-kill frequencies. Third, the road-kill frequency of Eurasian red squirrel can be reduced when the eco-corridor is located at the area across coniferous tree near 4 lane west sea freeway with mountain-road-mountain. Fourth, the road-kill of Raccoon dog can be reduced when the eco-corridor is located at 4 lane mountain road or hilly road with the geological type having farm land-road-mountain(stream). Fifth, Korean hare's road-kill can be reduced when the eco-corridor is located at grass land across ridge line of mountain, because wild rabbit road kill was happened at 4 lane mountain road or 2 lane mountain road(mountain-road-mountain). Sixth, As for Siberian chipmunk, the eco-corridor might be located at the side slope of mountain road at 2 lane mountain road under the speed of 60km/h with mountain-road-mountain. Seventh, For Water deer, the eco-corridor might be located at 4 lane hilly road with mountain-road-farm land. As for Common otter, Amur hedgehog, Yellow-throated marten, Weasel, it is difficult to specify the proper site of eco-corridor due to the lack of data. Eco-corridors for carnivores might be well located at 4 lane hilly road or 2 lane hilly road with mountain-road-farm land, and the track for herbivores might be well located as a overhead bridge on mountain-road-mountain type across mountains. In order to position eco-corridors for wildlife properly, we have to research animal's behavior with ecological background, and to consider the local uniqueness and regularly collect the empirical road-kill data in long term 3 to 5 year, which can be the foundation for the more suitable place of wild life eco-corridors.

• Korean Journal of Environmental Biology
• /
• v.31 no.1
• /
• pp.53-59
• /
• 2013

This study investigated mammal fauna in the sabbatical area of Mt. Unmun, Kyungsangbuk-do from December, 2007 to September, 2009. A total of 24 species belonging to 11 Families, 6 Orders, were identified in the survey area. Dominant species were as follows: Chinese water deer (Hydropotes inermis), Siberian chipmunk (Tamias sibiricus), Korean mole (Mogera wogura), and East Asian field mouse (Apodemus peninsulae). Especially, four Legal Protection Species specified as National Monument (NM) and Endangered Species (ES) found in this survey were as follows: Flying squirrel (Petromys volans; NM #328 and ES level II), Eurasian otter (Lutra lutra; NM #330 and ES level I), Leopard cat (Prionailurus bengalensis; ES level II) and Marten (Martes flavigula; ES level II). These results on the geographical distribution of various mammal species and Legal Protection Species indicate that this area is a valuable ecosystem for native mammal species. On the other hand, many feral cats were also found and specified as a management species. The results of this study show that further long-term investigation and management plan are needed to protect against harmful species and maintain the native ecosystem of Mt. Unmun.

• Korean Journal of Environment and Ecology
• /
• v.37 no.6
• /
• pp.418-428
• /
• 2023

This study was conducted to clarify the daily activity patterns overlap between hikers and mammals from 2015 to 2019 in the Baekdudaegan protected area. To investigate relationship behaviors between hikers and mammals, we set the camera traps on the ridge of the Baekdudaegan protected area. Daily activity patterns of yellow-throated marten (Martes flavigula) and Siberian chipmunk (Eutamias sibiricus) were highly overlapped with hiker total study periods. Moreover, daily activity patterns of Siberian roe deer (Caperohus pygargus) and water deer (Hydropotes inermis) were highly overlapped with hikers only in spring. In winter, daily activity patterns of wild boar (Sus scrofa) were overlapped with hikers. However, leopard cat (Prionailurus bengalensis), raccoon dog (Nyctereutes procyonoides), and Eurasian badger (Meles leucurus) did not significantly overlap with hikers during the study periods. The daily activity patterns of 8 mammals differed by species-specific behavior and temporal characteristics. Overlap of daily activity patterns between mammals and hikers were differed in each season. Differences in daily activity pattern overlap between mammals and humans may lead to differences in human impact on mammal populations. Information on the interaction between hikers and mammals on species-specific and temporal-specific behavior could be basic ecological data for management and conservation of mammal populations and their habitats.