• Journal of Ginseng Research
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• v.41 no.4
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• pp.444-449
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• 2017

The development of molecular markers is one of the most useful methods for molecular breeding and marker-based molecular associated selections. Even though there is less information on the reference genome, molecular markers are indispensable tools for determination of genetic variation and identification of species with high levels of accuracy and reproducibility. The demand for molecular approaches for marker-based breeding and genetic discriminations in Panax species has greatly increased in recent times and has been successfully applied for various purposes. However, owing to the existence of diverse molecular techniques and differences in their principles and applications, there should be careful consideration while selecting appropriate marker types. In this review, we outline the recent status of different molecular marker applications in ginseng research and industrial fields. In addition, we discuss the basic principles, requirements, and advantages and disadvantages of the most widely used molecular markers, including restriction fragment length polymorphism, random amplified polymorphic DNA, sequence tag sites, simple sequence repeats, and single nucleotide polymorphisms.

• 한국HCI학회:학술대회논문집
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• 2009.02a
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• pp.493-496
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• 2009

Many AR (Augmented Reality) applications have been interested in a marker-less tracking since the tracking methods give camera poses without attaching explicit markers. In this paper, we propose a new marker-less page recognition and tracking algorithm for an AR book application such as DigilogBook. The proposed method only requires orthogonal images of pages, which need not to be trained for a long time, and the algorithm works in real-time. The page recognition is done in two steps by using SIFT (Scale Invariant Feature Transform) descriptors and the comparison evaluation function. And also, the method provides real-time tracking with 25fps ~ 30fps by separating the page recognition and the frame-to-frame matching into two multi-cores. The proposed algorithm will be extended to various AR applications that require multiple objects tracking.

• The Journal of Korean Medicine
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• v.31 no.6
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• pp.8-15
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• 2010

Objectives: We investigated to develop and validate HPLC-PDA methods for simultaneous determination of eight constituents in Galgeun-tang (GGT). Methods: Reverse-phase chromatography using a Gemini C18 column operating at $40^{\circ}C$, and photodiode array (PDA) detection at 230 nm, 254 nm, and 280 nm, were used for quantification of the eight marker components of GGT. The mobile phase using a gradient flow consisted of two solvent systems. Solvent A was 1.0% (v/v) aqueous acetic acid and solvent B was acetonitrile with 1.0% (v/v) acetic acid. Results: Calibration curves were acquired with $r^2$ > 0.9999, and the relative standard deviation (RSD) values (%) for intra- and inter-day precision were less than 3.0%. The recovery rate of each component was in the range of 87.33-101.38%, with an RSD less than 7.0%. The contents of the eight components in GGT were 1.98-12.17 mg/g. Conclusions: The established method will be applied for the quantification of marker components in GGT.

• Proceedings of the Korean Society of Computer Information Conference
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• 2013.07a
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• pp.61-64
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• 2013

본 논문에서는 모바일 기반 OpenCV 라이브러리를 이용한 마커리스 객체 인석 성능 향상을 위한 소프트웨어적인 관점의 방법을 제안한다. 기존의 마커리스 기반 알고리즘을 이용하여 테스트를 수행한 후 성능에 저하를 발생시키는 요인들을 분석하고 그에 따른 상황별 적절한 해결책을 제시한다. 이에 따라 크게 프로그램 코드 개선, 마커리스 기반 알고리즘 코드 개선, 센서를 활용한 성능 향상을 도모한다. 프로그램 코드 개선은 테스트 결과를 분석 한 후 수행시간이 가장 많이 소요되는 함수를 최적화하고 또한 최적의 특징점의 수를 제한한다. 마커리스 기반 알고리즘 코드 개선은 병렬 처리가 제공되는 모바일에 한하여 병렬처리기법으로 코드를 수정한다. 마지막 센서를 활용한 성능향상은 실시간 작업 처리 단위를 묶음으로 처리하였을 때 발생하는 품질의 저하를 보정하는 역할을 수행한다. 본 논문에서는 이러한 마커리스 객체 인식 성능 향상 방법을 소프트웨어적인 관점에서 제안하고 이에 대한 결과 모바일 기반 실시간 증강현실 서비스를 위한 성능 향상 면에서 효과적이다.

• Journal of Korean language education
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• v.23 no.1
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• pp.1-23
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• 2012

The development of corpus in recent years has attracted increased research on spoken Korean. Nevertheless, these research outcomes are yet to be meaningfully and adequately reflected in Korean language textbooks. The reported speech marker '-dae' is one of these areas that need more attention. This study investigates whether or not in textbooks '-dae' is clearly explained to English-speaking learners to prevent confusion and misuse. Based on a contrastive analysis of Korean and English, this study argues three points: Firstly, '-dae' should be introduced to Korean learners as an independent sentence ender rather than a contracted form of '-dago hae'. Secondly, it is necessary to teach English-speaking learners that '-dae' is not equivalent to the English report speech form. It functions more or less as a third person marker in Korean. Learners should be informed that '-dae' is used for statements in English, if those statements were hearsay but the source of information does not need to be specified. This is a very distinctive difference between Korean and English and should be emphasized in class when 'dae' is taught. Thirdly, '-dae' should be introduced before indirect speech constructions, because it is mainly used in simple statements and the frequency of '-dae' is very high in spoken Korean.

• International Journal of Industrial Entomology and Biomaterials
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• v.15 no.2
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• pp.145-155
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• 2007

Mulberry (Morus spp.) is an economically important tree crop being cultivated in India, China and other sericulturally important countries for its foliage to feed the silk producing insect Bombyx mori L. Genetic improvements of mulberry lag behind to the same in many other economically less important crops due to the complexity of its genetics, the breeding behavior, and the lack of basic information on factors governing important agronomic traits. In this review, the general usage and advantages of different molecular markers including isoenzymes, RFLPs, RAPDs, ISSRs, SSRs, AFLPs and SNPs are described to enlighten their applicability in mulberry genetic improvement programs. Application of DNA markers in germplasm characterization, construction of genetic linkage maps, QTL identification and in marker-assisted selection was also described along with its present status and future prospects.

• Proceedings of the Korean Institute of Building Construction Conference
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• 2023.05a
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• pp.205-206
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• 2023

This paper presents a new method for indoor localization track workers in construction sites. While GPS and NTRIP are effective for outdoor positioning, they are less accurate when used indoors. To address this issue, the proposed method utilizes Aruco markers to measure the distance between workers and the markers. By collecting data values, the location of each worker can be determined in real-time with high accuracy. This approach has the potential to enhance work efficiency and safety at construction sites, as it provides more precise indoor positioning compared to conventional methods, leading to improved work efficiency.

• Journal of Ginseng Research
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• v.36 no.3
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• pp.298-307
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• 2012

Panax ginseng has been cultivated for centuries, and nine commercial cultivars have been registered in Korea. However, these nine elite cultivars are grown in less than 10% of ginseng fields, and there is no clear authentication system for each cultivar even though their values are higher than those of local landraces. Here, we have developed 19 microsatellite markers using expressed gene sequences and established an authentication system for all nine cultivars. Five cultivars, 'Chunpoong', 'Sunpoong', 'Gumpoong', 'Sunun', and 'Sunone', can each be identified by one cultivar-unique allele, gm47n-a, gm47n-c, gm104-a, gm184-a (or gm129-a), and gm175-c, respectively. 'Yunpoong' can be identified by the co-appearance of gm47n-b and gm129-c. 'Sunhyang' can be distinguished from the other eight cultivars by the co-appearance of gm47n-b, gm129-b, and gm175-a. The two other cultivars, 'Gopoong' and 'Cheongsun', can be identified by their specific combinations of five marker alleles. This marker set was successfully utilized to identify the cultivars among 70 ginseng individuals and to select true F1 hybrid plants between two cultivars. We further analyzed the homogeneity of each cultivar and phylogenetic relationships among cultivars using these markers. This marker system will be useful to the seed industry and for breeding of ginseng.

• The Korean Journal of Nuclear Medicine Technology
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• v.13 no.3
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• pp.185-188
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• 2009

Purpose: Standard of retests were discrepant and inconsistent due to inaccuracy and lack of standardization within normal range limit of tumor marker test. To enhance the standardization of retests set standard value below normal range and the Order Communication System Quality Control (OCS QC) program was put in place. This program enables managing the results within lower limit of normal range which were used for tumor marker test in Health Center. Materials and Methods: At present the tumor marker study for AFP, CEA, CA19-9, CA125, and PSA included outpatients in Asan Medical Center from February to March, 2009. The standard value was obtained by using the percentage of CV of Inter Assay according to the normal range of each tumor test. The results were confirmed by using the OCS QC program via formatted assessment of screening test such as test items, standard value and medical department. The number of out-of-range results within plus and minus 30 percents regarding the five primary items of tumor marker test was assessed. The next step was to obtain the number of AFP, CEA, and CA125 according to the ratio of comparison between prior and post test result, 60%, 50%, and 40% within normal range, respectively. In addition, set standard value below normal range. Results: The first screening test with percentage of sample number was resulted between 30%-40% and the second one was AFP 26.1%, CEA 18.9%, CA19-9 17.3%, CA125 18.7%, and PSA 21.0% obtained screening percentage of average 20 percents. The limited value of retest was AFP less than 5.0 and more than 10.0, CEA less than 1.0 and more than 3.0, CA19-9 less than 10.0 and more than 30.0, and PSA less than 1.0 and more than 2.0 to set and the number of retest was obtained by applying to the limited value of retest to screening percentage of average 20 percents For two months, the number of retest was AFP 0, CEA 15, CA19-9 3, CA125 2, and PSA 5. Conclusions: Through using the OCS QC program in establishing the standard of retest systemically, there appeared to be reduced discrepancy among the examiners and to be expected improvement in relation to the error of results.

• Radiation Oncology Journal
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• v.29 no.2
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• pp.91-98
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• 2011

Purpose: To assess the usefulness of implanted fiducial markers in the setup of hypofractionated radiotherapy for prostate cancer patients by comparing a fiducial marker matched setup with a pelvic bone match. Materials and Methods: Four prostate cancer patients treated with definitive hypofractionated radiotherapy between September 2009 and August 2010 were enrolled in this study. Three gold fiducial markers were implanted into the prostate and through the rectum under ultrasound guidance around a week before radiotherapy. Glycerin enemas were given prior to each radiotherapy planning CT and every radiotherapy session. Hypofractionated radiotherapy was planned for a total dose of 59.5 Gy in daily 3.5 Gy with using the Novalis system. Orthogonal kV X-rays were taken before radiotherapy. Treatment positions were adjusted according to the results from the fusion of the fiducial markers on digitally reconstructed radiographs of a radiotherapy plan with those on orthogonal kV X-rays. When the difference in the coordinates from the fiducial marker fusion was less than 1 mm, the patient position was approved for radiotherapy. A virtual bone matching was carried out at the fiducial marker matched position, and then a setup difference between the fiducial marker matching and bone matching was evaluated. Results: Three patients received a planned 17-fractionated radiotherapy and the rest underwent 16 fractionations. The setup error of the fiducial marker matching was $0.94{\pm}0.62$ mm (range, 0.09 to 3.01 mm; median, 0.81 mm), and the means of the lateral, craniocaudal, and anteroposterior errors were $0.39{\pm}0.34$ mm, $0.46{\pm}0.34$ mm, and $0.57{\pm}0.59$ mm, respectively. The setup error of the pelvic bony matching was $3.15{\pm}2.03$ mm (range, 0.25 to 8.23 mm; median, 2.95 mm), and the error of craniocaudal direction ($2.29{\pm}1.95$ mm) was significantly larger than those of anteroposterior ($1.73{\pm}1.31$ mm) and lateral directions ($0.45{\pm}0.37$ mm), respectively (p<0.05). Incidences of over 3 mm and 5 mm in setup difference among the fractionations were 1.5% and 0% in the fiducial marker matching, respectively, and 49.3% and 17.9% in the pelvic bone matching, respectively. Conclusion: The more precise setup of hypofractionated radiotherapy for prostate cancer patients is feasible with the implanted fiducial marker matching compared with the pelvic bony matching. Therefore, a less marginal expansion of planning target volume produces less radiation exposure to adjacent normal tissues, which could ultimately make hypofractionated radiotherapy safer.