• Title/Summary/Keyword: Marine Fish

Search Result 2,699, Processing Time 0.034 seconds

Development of Eggs, Larvae and Juveniles of the Squalidus gracilis majimae from Wicheon Stream, Nakdong-River (낙동강 수계 위천에 서식하는 긴몰개 Squalidus gracilis majimae의 난발생 및 자치어 형태발달)

  • Jae-Min Park;Kyeong-Ho Han
    • Korean Journal of Ichthyology
    • /
    • v.34 no.4
    • /
    • pp.244-252
    • /
    • 2022
  • The aim of this study was to investigate the early life history of Squalidus gracilis majimae by observing the development of the eggs and morphology of juvenile fish. Squalidus gracilis majimae used in this study were captured in 2021 June from a tributary flowing into Wicheon, located in Uiseong-gun, Gyeongsangbuk-do. For artificial insemination, ovaprim was injected at 0.5 mL/kg. Egg development occurred 12 h after injection. The size of mature eggs of S. gracilis majimae was 0.80~0.85 mm (0.83±0.01 mm, n=30). Incubation time at 22±1℃ ranged from 62.5 to 69 h. The length of larvae immediately after hatching was 2.39~2.53 mm (2.44±0.04 mm, n=30). On the 18th day after hatching, the larvae had a total length of 8.51~9.84 mm (8.95±0.42 mm, n=30), and the tip of the spine was completely curved. On the 22nd day after hatching, the juvenile fish had a total length of 12.6~13.6 mm (13.0±0.36 mm, n=30), with dorsal fins at iii7 and anal fins at i7. Egg size and hatched larvae size were similar for S. gracilis majimae and S. multimaculatus, and cupulae showed a singularity as it disappeared last among related species.

The A Literary Investigation on Mandu (Dumpling);Types and Cooking Methods of Mandu (Dumpling) During the Joseon Era (1400's${\sim}$1900's) (만두의 조리방법에 대한 문헌적 고찰;조선시대 만두의 종류와 조리방법에 대한 문헌적 고찰(1400년대${\sim}$1900년대까지))

  • Bok, Hye-Ja
    • Journal of the Korean Society of Food Culture
    • /
    • v.23 no.2
    • /
    • pp.273-292
    • /
    • 2008
  • Among all the ingredients usedin mandu, the following types were used:, 13 types of grains were used (12.38%), 30 types of vegetables, fruits, bulbs,and nuts were used (28.57%), 32 types of marine products, birds, meats, fishes, and shellfishes were used (30.48%), 10 types of functional ingredients were used (9.52%) and. For spices, 20 types of spices were used (19.05%). 2. Cooking Methods offor Mandu. The mMandu eaten at in the early Joseon era had was primarily made ofusedbuckwheat that contained boiled tofu or egg uiijuk in the kneaded dough for the most part and while kneading with buckwheat, the tofu or egg uiijuk has been boiled down to knead the dough, and and starch powder, bean powder, or rice powder, etc were mixed to make the mandu coating. Buckwheat powder was mixed toadded to the flourwer or was used by itself, while meat, vegetables, tofu, and shiitake mushroom, etc were also addedincluded. From the 18th century, the host plant, or cabbage kimchi, were prepared and combined had been sliced to be used as filling together while red pepper powder was mixed combined withto spices or vinegar soy sauce to be used together. Also, Radishes had beenwere also used as filling, but shown as not being used fromafter the start of the 1900's. For the shape of mMandu, it was madeinto different shapes such as as triangle, rectangle, date plum, gwebul, half moon, or pomegranate shapes, and then shapes to be boiled in simmering water, baked, or cooked as soup in clear broth for soup., In the 17th to 18th century, boilingthen in a steamer gradually became a cooking style, assumed the style of boiling in a steamer in $17th{\sim}18th$ century while in the 16th century,the an essay ofn fermenting flour in ‘Food Dimibang’ in 16th century had indicated it was cooked as the style ofby steaming in a rice steamer. Also, Mandu may have also contained the following: the thin-cut and boiled fish was cut out thin to put into the filling and boiled down, made by putting in added pine nuts after making bbeef jerky or boiled- down meat, fish, or shellfish itself to extractsand mold mandu only the ingredients combined withto put on starch powder, and then boiled down and put on pine nut powder finally, after it or cooled it wasdown to be eaten by dipping in vinegar soy sauce. In conclusion, many different types of mandu were made during the Joseon era using a variety ofwhile the ones using such various ingredients. are also one type of mandu.

Sequence Analysis of CO1 Genes of Fishery Resources from the Yellow Sea (eDNA 분석을 위한 황해 주요 수산자원의 CO1 염기서열 분석)

  • Hyun Sagong;Joo Myun Park;Yeonjung Lee;Wonseok Yang;Soo Jeong Lee;Maeng Jin Kim;Dong Han Choi
    • Ocean and Polar Research
    • /
    • v.46 no.3
    • /
    • pp.131-142
    • /
    • 2024
  • Ocean change due to anthropogenic activities and climate change are causing a decline in coldwater fish species and emergence of subtropical fish species in Korean waters. Therefore, environmental change-dependent time-space distribution of fishery resources in Korea, which has a big fisheries industry, needs to be investigated. Environmental DNA (eDNA) metabarcoding is an environmentally noninvasive method for understanding the spatiotemporal distribution of marine organisms at high spatial resolution. The highly variable cytochrome oxidase-1 (CO1) gene is used in eDNA studies for species identification across diverse taxa. However, it exhibits genetic differences depending on geographical distribution. For improving the accuracy of eDNA research, the CO1 database should be expanded by incorporating sequence information for individuals inhabiting the Korean seas. Here, 106 biological samples from the Yellow Sea were identified morphologically and their nucleotide sequences were compared with those in the GenBank. Most sequences were 100% identical with those in the GenBank. In most samples, the morphological and molecular identification results were consistent, indicating the utility of CO1. However, some nucleotide sequences differed from those in the database. Amino acid sequences translated from nucleotide sequences with less than 97% similarity showed high similarity to the amino acid database, indicating intraspecies variation due to "silent mutations". These results highlight the need for a sequence database of fishery resources in Korean coastal waters to improve the reliability of eDNA studies using CO1. However, because of the same CO1 sequences in several species, genetic markers need to be developed and the database should be supplemented with more sequences for reliable high-resolution eDNA studies.

Effects of the Various Dietary Additives on Growth and Tolerance of Abalone Haliotis discus hannai against Stresses (다양한 사료첨가제 공급에 따른 전복의 성장과 스트레스에 대한 내성 효과)

  • Cho, Sung-Hwoan;Kim, Chung-Il;Cho, Young-Jin;Lee, Bom-Sok;Park, Jung-Eun;Yoo, Jin-Hyung;Lee, Sang-Min
    • Journal of Aquaculture
    • /
    • v.21 no.4
    • /
    • pp.309-316
    • /
    • 2008
  • Effects of the various dietary additives on growth and tolerance of abalone Haliotis discus hannai to the stresses were determined in the 16-week feeding trial. Seventy juvenile (an initial body weight of 4.2 g) abalone per container were randomly distributed into 21, 50 L plastic rectangular containers each. The six kinds of experimental diets were prepared: control (CON) with no additive, by-product of green tea (BPG), extract of figs (EF), extract of green tea (EG), commercially available product of Hearok (PH), and Haematococcus (HC). In addition, dry sea tangle (ST) was prepared to compare the efficiency of the experimental diets. Fishmeal, soybean meal and shrimp head meal were used as the protein source, and dextrin, sea tangle powder and wheat flour, and soybean oil and fish oil were used as the carbohydrate and lipid sources, respectively in the experimental diets. The experimental diets were fed to abalone once a day at a satiation level with a little leftover. The feeding trial lasted for 16 weeks. At the end of the 16-week feeding trial, abalone was exposed to the different types of stresses (air exposure, and sudden changes of rearing temperature and salinity). Survival of abalone fed the sea tangle was highest. However, weight gain of abalone fed the EF, EG and PH diets was significantly (P<0.05) higher than that of abalone fed the BPG diet or dry sea tangle. Shell length of abalone fed the all experimental diets was significantly (P<0.05) higher than that of abalone fed the dry sea tangle. Accumulated mortality of abalone fed the sea tangle was low when exposed to the different types of stresses. Also, relatively low mortality was achieved in abalone fed the HC and EF diets. In considering these results, it can be concluded that the various sources of additives is effective to improve production of abalone, and Haematococcus and extract of figs can be considered as dietary additives to improve resistance of abalone against the different types of stresses.

Production of γ-amino Butyric Acid by Lactic Acid Bacteria in Skim Milk (탈지방우유에서 가바생성 유산균 배양을 통한 가바생성 연구)

  • Cha, Jin Young;Kim, Young Rok;Beck, Bo Ram;Park, Ji Hun;Hwang, Cher Won;Do, Hyung Ki
    • Journal of Life Science
    • /
    • v.28 no.2
    • /
    • pp.223-228
    • /
    • 2018
  • Lactic acid bacteria were isolated from a variety of fermented seafoods and sea creatures from the East Sea Rim, Korea and were screened for ${\gamma}-amino$ butyric acid-producing (GABA) activity. Through a 16S rRNA sequence analysis, the bacteria of interest, which were GABA-positive on the thin-layer chromatography analysis, were recognized as three isolates of Lactobacillus (Lb.) brevis and one isolate of Lactococcus (Lc.) lactis. Lb. brevis FSFL0004 and FSFL0005 were isolated from fermented anglerfish and Lb. brevis FSFL0036 was derived from salted cutlass fish. The Lc. lactis strain FGL0007 was isolated from the gut of a brown sole flounder. According to HPLC analysis, the GABA contents produced by FSFL0004, FSFL0005, FSFL0036 and FGL0007 were equivalent to $10,754.37{\mu}g/ml$, $13,082.79{\mu}g/ml$, $12,290.19{\mu}g/ml$, and $45.07{\mu}g/ml$ respectively in 1% monosodium glutamate-supplemented methionyl-tRNA synthetase (MRS) broth. The four strains were inoculated in skim milk with 1% monosodium glutamate to commercialize the strains as starter cultures for GABA-enriched dairy products, and TLC results displayed the production of ${\gamma}-amino$ butyric acid by all four strains in the adaptation media. Lc. lactis FGL0007 demonstrated the greatest GABA production ($431.42{\mu}g/ml$) by HPLC analysis. The GABA production by lactic acid bacteria strains in the skim milk demonstrated in the present study may be helpful for the production of GABA-enriched dairy products.

The Early Life History of the Rockfish, Sebastes inermis -1. Egg Development and Morphology of Larvae by Artificial Treatment in Aquarium- (볼락, Sebastes inermis의 초기생활사에 관한 연구 -1. 인위적 방법에 의한 수조내에서의 난발생과정과 부화자어의 형태-)

  • KIM Yong-Uk;HAN Kyeong-Ho
    • Korean Journal of Fisheries and Aquatic Sciences
    • /
    • v.26 no.5
    • /
    • pp.458-464
    • /
    • 1993
  • From November 1992 to January 1993, the rockfish, Sebastes inermis was reared in the laboratory, and observed the developmental stage of the eggs and larvae squeezed from the parent fish. On December 4 1992, developing eggs at morula stage were obtained from female parent fishes($17.4{\sim}20.3mm$ in total lenght(TL)). The diameter of eggs ranged from 1.20 to 1.35mm(n=50), and oil globules in white color were found in the eggs. Hatching began about 55 hours after morula stage at the water temperature of $9.2{\sim}12.3^{\circ}C$. The newly hatched larvae measured from 2.90 to 3.35mm in TL with $8+12{\sim}13=20{\sim}21$ myomeres. Total lengths of the larvae will be from 5.75 to 6.15mm in 27 and 30 days after the hatching, respectively. Myomere number was $8+17{\sim}18=26$.

  • PDF

Effects of Myostatin Prodomains on the Reproduction of Rotifer Brachionus rotundiformis (Myostatin prodomains이 rotifer 생활사에 미치는 영향)

  • Jo, Mi-Jin;Jin, Hyung-Joo
    • Journal of Life Science
    • /
    • v.22 no.4
    • /
    • pp.486-491
    • /
    • 2012
  • Myostatin (MSTN), a member of the transforming growth factor (TGF)-beta family, is a potent negative regulator of skeletal muscle growth and maintenance. The MSTN prodomain inhibits MSTN biological activity. The rotifer Brachionus rotundiformis is an excellent primary live feed for fish larvae in aquaculture; however, it is not known whether the rotifer expresses MSTN and the MSTN prodomain along with its activity. The objective of this study was to examine the effects of recombinant MSTN prodomains. Individual cultures of the rotifer B. rotundiformis were carried out to determine the effect of recombinant MSTN prodomains (pMALc2x-poMSTNpro and pMALc2x-sMSTNpro) on the pre-reproductive phase, reproductive phase, post-reproductive phase, offspring, lifespan, fecundity, and male ratio. In addition, a population culture of the rotifer was performed to confirm the effects of pMALc2x-poMSTNpro and pMALc2x-sMSTNpro on population growth. The results showed that the rotifer treated with pMALc2x-pMSTNpro had a reduced pre-reproductive phase at higher concentrations (1, 2, and 4 ${\mu}g/ml$) compared to the non-treated control group. Moreover, the pMALc2xsMSTNpro treated rotifer effectively decreased the pre-reproductive phase at a lower concentration (0.25 ${\mu}g/ml$) compared to the pMALc2x-pMSTNpro treated and control group. Interestingly, pMALc2x-poMSTNpro and pMALc2x-sMSTNpro significantly increased the population of $B.$ $rotundiformis$.

Studies on the Electrophoretic Analysis of Protein, Variations of Protein and RNA Contents of the Liver and Muscle Tissues of Bolephthalmus pectinirostris(Linnaeus) (짱뚱어, Boleophthalmus pectinirostris(Linnaeus)의 간근육조직내(肝筋肉組織內) 단백질(蛋白質) 전기영동(電氣泳動) 분석(分析) 및 단백질(蛋白質), RNA 함량(含量) 변화(變化))

  • Chung, Ee-Yung;Lee, Min-Woong;Lee, Keun-Kwang;Choi, Soo-Kyeong;Oh, Young-Nam
    • Korean Journal of Ichthyology
    • /
    • v.4 no.1
    • /
    • pp.96-104
    • /
    • 1992
  • The electrophoretic analysis, variations of protein and nucleic acid contents with sexual maturation, of the liver tissue of male, and of the muscle tissue of male and female B. pectinirostris(Linnaeus) were investigated. As the result of protein analysis of the liver and muscle tissue of male by the SDS-PAGE electrophoretic analysis, the protein of the liver tissue of male was presented 15.8-101.5kd in molecular weight, and appeared 19 bands in all. The protein bands of the muscle tissues of male and female were almost same, their bands were presented 16.9-116.5kd in molecular weight, and appeared 23 bands in all. And in the liver tissue, the protein contents of the liver tissue of male appeared the maximum in June(the mature stage) and afterwards decreased gradually in July(the spawning stage) and August(the degenerative stage). In the muscle tissues of male and female protein contents were shown the maximum in June, and afterwards decreased gradually, the minimum in July, increased a little in August. The total RNA contents of the liver tissue of male, and their contents of the muscle tissue of male and female appeared the maximum in June(the mature stage), and afterwards decreased gradually in July(the spawning stage) and August(the degenerative stage).

  • PDF

Change of Growth and Blood Characteristics on Starvation of Red Seabream Pagrus major Cultured in Marine Net Cage in Summer (하절기 가두리양식 참돔의 절식에 따른 성장과 혈액성상의 변화)

  • Kim, Won Jin;Won, Kyoung Mi;Shin, Yun Kyung;Lee, Jeong Yong
    • Korean Journal of Ichthyology
    • /
    • v.29 no.3
    • /
    • pp.165-175
    • /
    • 2017
  • To minimize the damage caused by summer season such as high temperature and red tide, this study was conducted to investigate the effect of feeding and starvation on the survival rate, growth, growth restoration and physiological response of red seabream Pagrus major which were reared in marine net cage ($6m{\times}6m{\times}6m$). The feeding group was fed throughout the experiment for 10 weeks, whereas starvation group was not fed for 6 weeks and subsequent refeeding for 4 weeks. Survival rates of feeding and starvation groups were 94.4% and 98.1% respectively. The growth rate of starvation group was significantly lower than feeding group during starvation period, but recovered rapidly after feeding. The nutritional status such as albumin, total protein, total cholesterol, triglyceride also showed similar tendency to the growth data. Hematocrit and hemoglobin levels were higher in the feeding group than the starvation group during starvation period. Aspartate aminotransferase, glucose and total cholesterol were rapidly increased at the same time (in 5 week) in feeding group, whereas starvation group did not significant difference during starvation period. It was shown that the feeding group is more sensitive to stress than the starvation group. Thus, starvation can be applied as a rearing management plan of net cage fish farm in summer season.

Morphological Study of the Genus Chromis from Korea II . Comparison of Skeletal Characters of Chromis notata, Chromis analis and Chromis fumea (한국산 자리돔속 어류의 형태학적 연구 II. 한국산 자리돔속 어류 3종, 자리돔 (Chromis notata), 노랑자리돔 (Chromis analis) 및 연무자리돔 (Chromis fumea)의 골격 비교)

  • KIM Yong Uk;KIM Jin Koo
    • Korean Journal of Fisheries and Aquatic Sciences
    • /
    • v.30 no.4
    • /
    • pp.562-573
    • /
    • 1997
  • Comparative osteology of Chromis notata, C. analis and C. fumea was studied based on the samples collected from the Korean waters. The three species are distinguished by the shape of cranium, visceral skeleton, vertebra and caudal skeleton. Chromis notata has a glossohyal which is slightly curved in shape while two other species are deeply curved. C. analis has three spiniform procurrent caudal rays while others have two. C. fumes could be distinguished easily by the shape of preopercle which is serrate in posterior margin. This investigator reveals following bones as new taxonomic criteria for the genus Chromis in Korea. In cranium, height of supraoccipital, height of process of parasphenoid and anterior margin of basisphenoid, in visceral skeleton, presence of fine papilla-like process at the surface of preopercular sensory canal, in orbital bone, width of preorbital, in hyoid arch, shape of glossohyal, union state between lower hypohyal and ceratohyal and union state between ceratohyal and epihyal, in shoulder girdle bone, dorsal margin of posttemporal, width of upper postclavicle, posterior end of lower postclavicle and posterior margin of first actinost bone, in vertebrae, beginning point of parapophysis, in caudal skeleton, upper shape of second neural spine of pleural centrum.

  • PDF